Engineered 3D Vascular Networks: Enabling technology for Vital Organ Engineering Complex liver microvasculat ure Construct synthetic vascular networks with thermally reversible polymers and microfluidics Aim 1 Aim 2 Cross- section Hepatocytes Engineer ed vascular networks Top Assemble alternating vascular and hepatocyte layers towards 3D synthetic vascularized liver construct Vascular-mimetic microfluidic channel Mold thermally reversible polymer containing endothelial cells culture Cross - secti on End goal: formed endothelial tube 200μm (Mary Beth Wilson, Dowd Fellowship 2010)