Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables.

Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes

João F. Mano

3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer 3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, Campus de Gualtar, 4710-057 Braga, PortugalEngineering, Campus de Gualtar, 4710-057 Braga, Portugal

IBB- Institute for Biotechnology and Bioengineering, Braga, PortugalIBB- Institute for Biotechnology and Bioengineering, Braga, Portugal

PhD Students/ Other Full time ResearchersAdriano PedroAlbino MartinsAna MendesAna PinheiroAna OliveiraAna SousaAna PintoAnabela PintoBarbara BertiniBrecht ClaesBruno FerreiraCatarina AlvesCornelia BrunnerDaniela CoutinhoElisabete PinhoElizabeth BalmayorEmanuel FernandesHelena LimaIsabel LeonorIvo ArosoJessica GrechJoana MagalhãesJoão OliveiraJoaquim FerreiraJoaquim OliveiraJosé Rodriguez

3B´s RESEARCH GROUP

Staff - Rui L. Reis (Director)- João F. Mano- Nuno M. Neves- Natália Alves

José PereiraJosé JúniorKadriye TuzlakogluLeandro GardelMárcia RodriguesMarina SantosMarta SilvaPatrícia BaptistaPaula SolPaula PérezPaulo BessaRicardo SilvaRogério SousaRosa BessadaSandra OsórioSangwong ChungSatyabrata GoshSílvia PereiraSimone SilvaSusana SantosTeresa SilvaTírcia SantosTommaso RadaVitor Espírito SantoVítor SilvaWojciech Szymczyk

Technical StaffAriana SantosBárbara BarrosoBerta AlmeidaFilipe RibeiroLiliana GomesRicardo GomesTânia Delalande AlvesVirgínia Araújo

Other PhD HoldersAlexandra MarquesAna GrenhaAna FriasAntónio SalgadoCarlos ViegasErkan BaranGabriela SilvaHelena AzevedoIsabel DiasIva PashkulevaJohan BeneschLuciano BoeselMaria Manuela GomesMazen TamerRicardo PiresRita SerraRui Amandi de SousaSandra SampaioSusana MartinsTiago SilvaZhongkui Hong

“Tissue Engineering (TE) is an interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function”

Langer and Vacanti., Science 260, 920 (1993)

TE: AN INTERDISCIPLINARY SCIENCE

Medical science

BiologyBiochemistry

Materials Science& engineeringChemistry TE

cells

+ Growth factors

CONSTRUCTION OF HUMAN TISSUE SUBSTITUTES

What material??•Biodegradable

polymers

Which structure ??

•Porous/interconected

Which processing method??

SCAFFOLD

Materials used at the 3B’s Group

Starch + EVOH or CA or PCL or PLA High potential for a all range of biomedical applications: - tissue engineering scaffolding - drug delivery carrier systems - bone replacement / bone defects; bone plates / screws; partially degradable bone cements

O

CH2OH

H

H

H

OH

NHCOCH3

HH

O

O

CH2OH

H

H

H

OH

NHCOCH3

HH

O

n

GlcNAc

O

CH2OH

H

H

H

OH

NH2

HH

O

O

CH2OH

H

H

H

OH

NH2

HH

O

n

CHITIN

Deacetylation

CHITOSAN

GlcNAc

+ CH3COONa

NaOH, temperature

Chitosan based materials

other polysaccharides, proteins and blends with biodegradable polyesters…

3B´s SCAFFOLD PROCESSING ROUTES3B´s SCAFFOLD PROCESSING ROUTES

Injection moulding with blowing agents Injection moulding with blowing agents

Extrusion with blowing agentsExtrusion with blowing agents

Microwave BakingMicrowave Baking

Fiber Bonding (Wet or Melt-spinning)Fiber Bonding (Wet or Melt-spinning)

Compression moulding and salt leachingCompression moulding and salt leaching

Solvent casting-particle leachingSolvent casting-particle leaching

In-situ polymerizationIn-situ polymerization

(In-situ) Enzymatic Degradation(In-situ) Enzymatic Degradation

Freeze dryingFreeze drying

Rapid prototypingRapid prototyping

………………

ceramic+chitosan bilayered scaffolds

polyurethane sponge

HA slurry

burningchitosan solution

freeze-drying

pore size: 50 – 350 µm

STEM /PROGENITOR CELLS• EMBRYONIC

• BONE MARROW

• ADIPOSE TISSUE

• UMBILICAL CORD

• AMNIOTIC MEMBRANE

Cell response to bilayered scaffoldsCell response to bilayered scaffolds

100 µm

20 m

cartilage region

bone region

condrogenic medium

osteogenic medium

goat marrow cellsgoat marrow cells

28 days

14 days

Isabel Dias, Carlos Viegas

Human Osteoblast-like cells (SaOs-2) and human fibroblasts

(MRC-5)

Cell encapsulation and co-cultures

Osteoblasts

Fibroblasts

CaCl2+NaCl

sodium alginate + Iota carrageenan

+ cells

+

---

-

-

---

-

-

++

+ ++

+

+

++

+

+

+

+

+

Polyanion

Ca2+ ions

Cells

SaOs-2 with CMC/PAMAM-FITC INTERNALIZATION OF INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLESCMC/PAMAM-FITC NANOPARTICLES

after 14 days

PAMAMPAMAMdendrimersdendrimers CarboxymethylCarboxymethyl--chitosanchitosan

++

CMCCMC//PAMAMPAMAM

DendronizedDendronized polymerpolymer

PAMAMPAMAMdendrimersdendrimers CarboxymethylCarboxymethyl--chitosanchitosan

++

CMCCMC//PAMAMPAMAM

DendronizedDendronized polymerpolymer

novelnovel carboxymethyl-chitosan/poly carboxymethyl-chitosan/poly (amidoamine)(amidoamine) (CMC/PAMAM) (CMC/PAMAM) dendronized polymersdendronized polymers (natural-based (natural-based polymers + dendrimers).polymers + dendrimers).

EU FUNDED PROJECTSEU FUNDED PROJECTS

STREP – HIPPOCRATES

Marie Curie EST - ALEA JACTA EST

NoE – EXPERTISSUES

Marie Curie SCF - InVENTS

• STREP – WaCheUp• IP-GENOSTEM• NANOPHOTOTECH• Marie Curie EST JoinTEd• Marie Curie: POLYSTEM

PROTEUSINTERREG IIIA

NEW JOURNAL - TERM

Editor-in-ChiefProf. Rui L. Reis

BiomaterialsBiomaterialsBiodegradablesBiodegradables

BiomimeticsBiomimetics

www.3bs.uminho.pt

[email protected]