Dna Extraction

DNA Intro

Warm-ups!O What is the chemical equation for

photosynthesis?O What is the chemical equation for cellular

respiration?O What are the steps of cellular respiration?

O Which step(s) do not require oxygen?O Where does each step occur in the cell?O *feel free to draw it out.

O What do you know about DNA and what do you want to know?

A VIDEOO http://www.youtube.com/watch?v=aQR_aaUqMPcO http://www.youtube.com/watch?v=aIyaxgBkToU&feature=related

DNA Extraction Lab

Lab

Title: DNA Extraction Lab

Purpose: To extract DNA and make general observations of DNA

MaterialsO One wooden stickO 1 pipetteO 1 50 ml conical tubeO 1 15ml conical tubeO Tube holder optionalO SoapO 20mL waterO Waste beakerO EthanolO Wheat Germ, Strawberry, Banana?

Make a Flow-gram

Mix the detergent once every min. for 5 min

Intro to DNA

Zooming into DNA

http://www.thetech.org/genetics/zoomIn/

http://www.pbs.org/wgbh/nova/genome/dna_flash.html

DNA stands for Deoxyribonucleic AcidThe structure of DNA is a double stranded helix

DNA is important because it codes for all of our traits.

http://www.biotechnologyonline.gov.au/popups/int_dnazoom.html

DNA is a type of nucleic acid made up of monomers called nucleotides

There are 3 parts to make a nucleotide

Warm up!!O What are the 3 parts of a nucleotide?O Which part makes each nucleotide differentO Work on your study guide until 8:50aTO DO:O Take out Photosynthesis and Cell Resp.

AnnotationsO Take out Field Trip form with 5$O Email Mackenzie at

[email protected] if you want study help after school

Field TripO On Tuesday of Next Week!! Yay!!!O You can bring a snack and waterO Wear comfortable clothing and shoes

we will be doing a lot of walking.

O The nitrogenous base is what makes each type of nucleotide different

O The phosphate and the ribose sugar make up the back bone of the DNA

There are 4 types of nucleotides that make up DNAAdenine GuanineCytosineThymine

http://www.youtube.com/watch?v=HvJlnujmYcg&feature=BFa&list=PL9CDDABF9F37094D9&lf=results_main

Chargaff’s Ratio shows that the number of Adenine is always equal to the number of Thymine and the number of Guanine is always equal to the number of Cysteine.

http://www.youtube.com/watch?v=HvJlnujmYcg&feature=BFa&list=PL9CDDABF9F37094D9&lf=results_mainhttp://www.youtube.com/watch?v=PDeaLxoL75M&feature=BFa&list=PL9CDDABF9F37094D9&lf=results_main

The nucleic acids of DNA pair up O A pairs with T using 2 hydrogen bondsO G pairs with C using 3 hydrogen bonds

O The strands of DNA are antiparallel.