Discovery: Stem Cell Biology NIH Actions Continue infrastructure award program Characterize cell lines Stimulate more research on basic biology Train.

Discovery: Stem Cell BiologyDiscovery: Stem Cell BiologyNIH ActionsNIH Actions

Continue infrastructure award programContinue infrastructure award program

Characterize cell linesCharacterize cell lines

Stimulate more research on basic Stimulate more research on basic biologybiology

Train investigators to culture and use Train investigators to culture and use stem cellsstem cells

NIHNIH AWARDSAWARDS

• 60 investigators at 40 institutions60 investigators at 40 institutions

• 14 investigator-initiated grants14 investigator-initiated grants

• 44 supplements44 supplements

hESC LINEShESC LINES

Number available Number available

for shipping increased for shipping increased

from 5 to 11 sincefrom 5 to 11 since

September 2002.September 2002.

NIH Supported NIH Supported ResearchResearch

University of WisconsinUniversity of Wisconsin scientists scientists replaced a specific stretch of DNA in replaced a specific stretch of DNA in hESC -- homologous recombinationhESC -- homologous recombination

Advance:Advance: Scientists can now study the Scientists can now study the function of specific genes within these function of specific genes within these cells and could modify hESC-derived cells and could modify hESC-derived tissues for potential treatment in patients.tissues for potential treatment in patients.

NIH Supported ResearchNIH Supported Research

NIH ScientistsNIH Scientists observe: differentiated observe: differentiated mESCs repaired damage when mESCs repaired damage when transplanted into the mouse brain or spinal transplanted into the mouse brain or spinal cord.cord.

Advance:Advance: May lead to development of May lead to development of replacement therapy for cells destroyed replacement therapy for cells destroyed by injury or disease, such as stroke, by injury or disease, such as stroke, Parkinson’s or Alzheimer’s disease.Parkinson’s or Alzheimer’s disease.


Advance: Advance: In vitro studies produced cells In vitro studies produced cells from hESC that might be used for blood from hESC that might be used for blood cell transplantation therapies for patients cell transplantation therapies for patients with blood malignancies such as leukemia with blood malignancies such as leukemia or myeloma.or myeloma.


Scientists are currently working to identify those Scientists are currently working to identify those genes that are involved in the differentiation of genes that are involved in the differentiation of hESCs and genes that permit embryonic stem hESCs and genes that permit embryonic stem cells to self-renew.cells to self-renew.

AdvanceAdvance:: Once the genes are identified, gene Once the genes are identified, gene transfer techniques may permit scientists to coax transfer techniques may permit scientists to coax hESCs into becoming insulin-producing beta hESCs into becoming insulin-producing beta cells to treat insulin-dependent diabetes.cells to treat insulin-dependent diabetes.


Scientists tested the ability of human Scientists tested the ability of human feeder cells derived from fetal or adult feeder cells derived from fetal or adult tissues to support the growth of hESC.tissues to support the growth of hESC.

AdvanceAdvance: This is an ideal system for : This is an ideal system for identifying factors secreted by human identifying factors secreted by human feeder cells that maintain hESCs’ self-feeder cells that maintain hESCs’ self-renewing and multipotent state.renewing and multipotent state.


U. of MinnU. of Minn. Scientist isolated multipotent . Scientist isolated multipotent adult progenitor cells from human bone adult progenitor cells from human bone marrowmarrow

Advance:Advance: Adult stem cells demonstrated Adult stem cells demonstrated the potential to differentiate beyond bone the potential to differentiate beyond bone marrow stem cells and into other cell marrow stem cells and into other cell types, including liver cells, neurons and types, including liver cells, neurons and blood vessel-forming cells.blood vessel-forming cells.


Stem cells found in dental pulp of “baby Stem cells found in dental pulp of “baby teeth” have the potential to become cells teeth” have the potential to become cells expressing molecular markers expressing molecular markers characteristic of dentin, bone, fat, and characteristic of dentin, bone, fat, and nerve cells.nerve cells.

AdvanceAdvance: These cells could possibly be : These cells could possibly be used to repair damaged teeth, regenerate used to repair damaged teeth, regenerate bone, treat nerve injury or disease.bone, treat nerve injury or disease.

NIH-supported researchNIH-supported research

Umbilical cord stem cells are able to Umbilical cord stem cells are able to repopulate the bone marrow of a small repopulate the bone marrow of a small child, but only a small number of cells are child, but only a small number of cells are obtained from each umbilical cord.obtained from each umbilical cord.

We are now seeking methods to expand We are now seeking methods to expand cells in culture to generate larger numbers cells in culture to generate larger numbers for use in clinical applications.for use in clinical applications.

Investigator-Initiated AwardsInvestigator-Initiated Awards

Modeling Development hematopoiesis with Modeling Development hematopoiesis with embryonic stem cells — embryonic stem cells — Whitehead InstituteWhitehead Institute

Strategies for Primate TransgenesisStrategies for Primate Transgenesis– U. of Wisconsin at MadisonU. of Wisconsin at Madison

Neurons from Human & MouseNeurons from Human & Mouse– Washington UniversityWashington University

In vivo potential of human ES-cell derived In vivo potential of human ES-cell derived blood cells – blood cells – University of MinnesotaUniversity of Minnesota

Gene expression in beta-cells by lentiviral Gene expression in beta-cells by lentiviral vectors – vectors – Harvard UniversityHarvard University

Growth factor-based culture system for ES Growth factor-based culture system for ES cells – cells – R and D System, Inc.R and D System, Inc.



Towards renal Towards renal regenerationregeneration - - U. of U. of Queensland, AUQueensland, AU

Cranial bone repair with adipose tissue-Cranial bone repair with adipose tissue-derived stem cells – derived stem cells – U.VaU.Va..

Derivation of smooth muscle lineages from Derivation of smooth muscle lineages from stem cells – stem cells – U.Va.U.Va.

Regulation of embryonic stem cells– Regulation of embryonic stem cells– Monash University, AUMonash University, AU


Radiation damage repair in the brain via Radiation damage repair in the brain via human ES cells – human ES cells – Sloan Kettering Sloan Kettering InstituteInstitute

Improved lentiviral vectors for primate ES Improved lentiviral vectors for primate ES cells– cells– U. of Wisconsin – MadisonU. of Wisconsin – Madison

Neural specification of embryonic stem Neural specification of embryonic stem cells cells – U. of Wisconsin– U. of Wisconsin

Discovery: Stem Cell Biology NIH Actions Continue infrastructure award program Characterize cell lines Stimulate more research on basic biology Train.

Documents

research stem cells

beta cells

nerve cells

liver cells

adult stem cells

bone marrow stem cells

primate es cells

es cells r