efinitive identification of all specie of the genus Anguilla using the complete mitochondrial genome Yuki Minegishi 1 , Jun Aoyama 1 , Jun G. Inoue 1 , Masaki Miya 2 , Mutsumi Nishida 1 , and Katsumi Tsukamoto 1 1 Ocean Research Institute, The University of Tokyo 2 Department of Zoology, Natural History Museum and Institute 1
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Definitive identification of all species of the genus Anguilla using the complete
Definitive identification of all species of the genus Anguilla using the complete mitochondrial genome. Yuki Minegishi 1 , Jun Aoyama 1 , Jun G. Inoue 1 , Masaki Miya 2 , Mutsumi Nishida 1 , and Katsumi Tsukamoto 1. 1 Ocean Research Institute, The University of Tokyo - PowerPoint PPT Presentation
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Definitive identification of all speciesof the genus Anguilla using the complete
mitochondrial genome
Yuki Minegishi1, Jun Aoyama1,Jun G. Inoue1, Masaki Miya2,
Mutsumi Nishida1, and Katsumi Tsukamoto1
1 Ocean Research Institute, The University of Tokyo2 Department of Zoology, Natural History Museum and Institute
1
Genetic methods for species identification
Takagi and Taniguchi (1995)Tagliavini et al. (1995)Wakao et al. (1999)Zhang et al. (1999)Aoyama et al. (2000)Lehmann et al. (2000) Lin et al. (2002)Watanabe et al. (submitted)
RAPD
PCR-RFLP
PCR-RFLP
PCR-RFLP
PCR-RFLP
RAPD
PCR-RFLP
Real time-PCR
Reference Method
Introduction-1
2
A. reinhardtii / A. mossambica(Bastrop et al., 2000)
A. marmorata / A. nebulosa labiata(Aoyama et al., 2001)
- A. bicolor bicolor - A. malgumora (=A. borneensis)
Same sequences
Different sequences
Introduction-2
Aoyama et al., 2001 / Lin et al., 2001
3
- To obtain the exact complete mitochondrial DNA sequencesof all species of the genus Anguilla
- To collect the basic data forpopulation genetics, molecularphylogeny, DNA taxonomy, etc.
Objective
4
Materials
1
3,82,16
4,145,6,7,10
9,11,1512
13
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1. A. anguilla2. A. australis australis3. A. australis schmidtii4. A. bicolor bicolor5. A. bicolor pacifica6. A. borneensis7. A. celebesensis8. A. dieffenbachii9. A. interioris10. A. marmorata11. A. megastoma12. A. mossambica13. A. nebulosa labiata14. A. nebulosa nebulosa15. A. obscura16. A. reinhardtii17. A. rostrata18. A. japonica (Inoue et al., 2001)Watanabe S. (2003)
Taxonomy ofthe freshwater eels, genus Anguilla, Schrank 1798