Course Contents 1. DNA and RNA isolation 2. Quantification of DNA and RNA 3. Primer designing 4. PCR 5. Electrophoresis 6. Sequencing 7. Karyotyping 8. Restriction Mapping 9. Flow cytometry 10.Hybridization a. Western blotting b. Southern blotting c. Northern blotting d. FISH
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Course Contents DNA and RNA isolation Quantification of DNA and RNA Primer designing PCR
Course Contents DNA and RNA isolation Quantification of DNA and RNA Primer designing PCR Electrophoresis Sequencing Karyotyping Restriction Mapping. Flow cytometry Hybridization Western blotting Southern blotting Northern blotting FISH. Tissue culturing - PowerPoint PPT Presentation
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Course Contents1. DNA and RNA isolation2. Quantification of DNA and RNA3. Primer designing4. PCR5. Electrophoresis6. Sequencing7. Karyotyping8. Restriction Mapping
9. Flow cytometry10.Hybridization
a. Western blottingb. Southern blottingc. Northern blotting d. FISH
11.Transfection
12.Transduction
13.Transformation
14.Cloning
15.Microarrays
16.Chromatography
17.Immunochemistry
18.ELISA
19.Bioinformatics and techniques
20.Ethical issues
21.Tissue culturing
22.Slide Preparation and Cell Stains
23.Agar plate preparation and streaking for
the purpose of individual colony isolation
24.Bacterial Growth on selective agar
25.Quantification: Colony Forming Units
(CFU)
26.Dilution Plating
27.Identification and characteristics of
colonies
Assignment
Presentation
Quiz
Transfection :
is the process of deliberately introducing nucleic acids into cells.
Transformation:
non-viral DNA transfer in bacteria, non-animal eukaryotic cells and plant cells.
Transduction: is often used to describe virus-mediated DNA transfer.
What to transferGenetic material (such as supercoiled plasmid DNA or siRNA constructs), or even proteins such as antibodies, may be transfected.
Target:Transfection of animal cells typically involves opening transient pores or "holes" in the cell membrane, to allow the uptake of material.
TRANSFECTION Transient TransfectionRapid, scalable, high-yield protein production from transiently transfected suspension cultures.the DNA introduced in the transfection process is usually not integrated into the nuclear genome, the foreign DNA will be diluted through mitosis or degraded.
Stable TransfectionStable transfection introduces DNA into cells long-term and pass the introduced DNA to their progeny.
marker gene is co-transfected, which gives the cell some selectable advantage, such as resistance towards a certain toxin
Gene name Gene product Assay
LacZ β-galactosidase enzyme assay or Histochemical