Chrom Journal 3 EN:ChromMagazine EUR.qxdes.vwr-cmd.com/ex/downloads/magazine/chromjournal... · VWR ChromJournal Issue 3 September 2007 3 Flash chromatography is the fast and inexpensive

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Chromolith® demonstratesspeed and accuracy for the Swedish University of Agricultural Sciences

pages 4 - 5

EZChrom EliteTM supports a single workstation up to a fully equipped and company-wide client/serversystem

pages 12 - 13

ISSUE 3 - SEPTEMBER 2007Innovative products for chromatography

ChromJournal

vwr.com

VWR Prolabo solvents areguaranteed to performeverytime

page 19

A reusable closed systemfor HPLC solvents

page 19

NEW AcroSep™

Chromatography Columnsfor Ion Exchange from PallLife Sciences

pages 22 - 23

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EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International Europe bvba

Layout and typesettingMarketing Services VWR

PrintingPRT, Paris, FranceNo part of this publication may be reproduced or copied withoutprior permission by writing of VWR International Europe.

Run45.000 copiesPublication date: September 2007

In this issue

Welcome to the Autumn edition of the VWR ChromJournal.

After what we hope has been an excellent summer for everyone, as the

days get shorter and the weather a bit colder and wetter the ChromJournal

aims to brighten your day by bringing you news and application stories

from all types of chromatography. If you’d like to contribute to the journal

or have any comments or suggestions about topics you would like included

in future issues then please email us at [email protected]

SPOT FLASH 03

Rapid separation of berry anthocyanins with Chromolith® column 04

Chromolith® HPLC columns convert standard HPLC systems into racehorses 06

Purospher STAR® HPLC columns - for successful separations 08

Hamilton PRP-h1 HPLC columns with extra sensitivity 10

Validation Manager - quick and reliable 11

EZChrom EliteTM software overview plus training dates for 2008 12

Navigator System for optimal HPLC 14

VWR, L-2130i inert pump - NEW 16

VWR, conductivity detector CD-5 - NEW 17

Chromeleon Drivers for LaChrom Elite® 18

Solvents - Purity, packaging and price the choices 19

Schott Duran® HPLC Laboratory bottles 20

SGE, Forte columns for abusive GC! - NEW 21

Pall AcroSep™ Chromatography Columns for Ion Exchange - NEW 22

VWR Collection storage vials - NEW 24

VWR C h r o m J o u r n a l Issue 3 S e p t e m b e r 2 0 0 72

ChromJournal editorial

VWR C h r o m J o u r n a l Issue 3 S e p t e m b e r 2 0 0 7 3

Flash chromatography is the fast

and inexpensive separation

technique for the purification

of organic syntheses products,

biological extracts and also for

food and environmental samples.

The new SPOT FLASH System

generation creates new scales

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and offers a great potential to

the user.

>> Pump• Very robust, high performance serial double

piston pump with a flow rate range up to300 ml/min with binary or quaternary lowpressure gradient and a pressure range up to25 bar

• Automatic flow rate adjustment related tothe set maximum back pressure

>> Sample Injection• Direct liquid injection on top of the column

without dead volume or with a pre-columnor optionally using an injection valve withloop

• Application of bulk sorbent loaded withsample on top of the column or via aseparate column

>> Column• All columns with appropriate fittings can be

connected: Columns packed with normalphase or reversed phase sorbents from 2,5 gup to 600 g or even more

• VWR supplies a broad range of cost-efficient,ready-to-use columns. Cartridges made ofhigh-purity polypropylene and compatiblewith all normal phase and reverse phasesolvent – e.g. filled with sorbents from Merckor other recognised suppliers

>> DetectorA choice of three different detectors isavailable • Filter photometer• Spectral-photometer with one wavelength• Spectral-photometer with dual wavelength

>> Fraction-Collector• Fraction collection by volume, time, signal

threshold and local minimum mode forautomatic fractionation of non-separatedpeaks

• Large number of fractions (5,6 l totalcapacity by using standard racks)

>> Software• The system is equipped with a computer and

user-friendly software for access to allparameters while a purification is in progress

• Further features are: Real time controlmethod including click & drag features forrun time, gradient and flow rate

• Automatic download of basicchromatography conditions related to theselected column by using a fully open columndatabase

SPOT FLASH

For more information on these products contact your local VWR sales office,send an e-mail to [email protected] or visit our website www.vwr.com

Flash chromatography

>

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Rapid separation of berry anthocyanins with Chromolith® columnsJohanna Witzell & Marie Lundström

The phenolic anthocyanin pigments are widely distributed in the plantkingdom. The red, deep purple and black berries, such as bilberries(Vaccinium sp.) are particularly rich in anthocyanins and provide animportant source of anthocyanins in the human diet. Recent researchhas underlined the potential antioxidant function of anthocyanins,which increases the nutritional importance of berries. From theindustrial point of view, the anthocyanins of berries are of currentinterest because of their importance as natural food colourants. In orderto reliably and quickly analyse the anthocyanin content of rawmaterials, the analysis methods need to be continuously evaluated andoptimised. Here, we tested if a reversed-phase HPLC method, previouslydescribed for analysis of anthocyanins in berries and wine (Nyman andKumpulainen 2001), could be made faster with the help of a monolithiccolumn.

Material and methodsGlycosides were extracted from the bilberries that were first frozen, freeze dried and milled to afine powder, using 2M HCl in methanol (shaking at +4 °C for 20 minutes). Aglycones werereleased from the same extracts by hydrolysis (90 °C in a water bath for 25 min). All samples werefiltered using disposable filters (0,22 μm pore size) before injection to HPLC.

A Merck Hitachi LaChrom HPLC system consisting of an L-7100 pump, an L-7200 autosampler, anL-7360 column oven and an L-7455 diode array detector were used. The oven temperature was setat 30 °C. The compounds were separated on a monolithic column (Chromolith®, Merck KGaA,Darmstadt, Germany) using a method modified from Nyman and Kumpulainen (2001). In ouranalyses, gradient elution was performed using 100% methanol (solution A) and 10% formic acid(solution B) as follows: 5% A in B (0-14 min), 40% A in B (15-19 min), followed by short flushingwith methanol and equilibration to initial conditions. Flow rate was 2 ml/min. The injection volumewas 25 μl for all samples and standard solutions. Peak areas from the analysed compounds weremeasured at 525 nm and identified by comparing their retention times and spectral characters tothose of reference compounds. Cyanidin, delphinidin, malvidin, peonidin and petunidin (chloridesalts) and cyanidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-rutinoside and delphinidin-3-glucoside standards were purchased from Extrasynthese (Geney, France) and used as externalstandards.

>

Analytical HPLC

Anthocyanins are glycosylated

phenolic compounds, which

after hydrolysis release their

aglycon (anthocyanidin).

Because of their use as natural

colourants and health

promoting agents,

anthocyanidins and

anthocyanins are of

considerable current interest for

pharmaceutical and food

industries. We studied

anthocyanidin and anthocyanin

profiles in berries of the

Swedish bilberry (Vaccinium

myrtillus). By using a monolithic

Chromolith® column we

obtained an excellent

separation of 17 anthocyanin or

anthocyanidin peaks. The five

characteristic anthocyanidins of

bilberry eluted within 13

minutes, with retention times of

delphinidin and malvidin being

7,0 and 12,6 minutes,

respectively. Thus, the

monolithic column allowed

rapid and accurate analysis

of bilberry anthocyanins and

anthocyanidins.

Figure 1.HPLC chromatogram of hydrolyzedsamples of Vaccinium myrtillusberries. 1 = delphinidin; 2 = cyanidin; 3 = petunidin; 4 = peonidin; 5 = malvidin.


ResultsIn the hydrolysed V. myrtillus berries, five characteristic aglycones, delphinidin, cyanidin, peonidin,petunidin and malvidin were found (Figure 1). The HPLC-chromatogram of the non-hydrolysedsamples resulted in a total of 17 peaks (Figure 2). Among these peaks, delphinidin-3-glucoside,cyanidin-3-galactoside, cyanidin-3-glucoside and aglycones petunidin, peonidin and malvidin wereidentified.

ConclusionThe use of the monolithic column allowed good separation of 17 anthocyanins or anthocyanidins.The five bilberry anthocyanidins eluted within 13 minutes with retention times of delphinidin andmalvidin being 7,0 and 12,6 minutes, respectively, instead of about 10 and 20 min as reportedearlier (Nyman and Kumpulainen 2001). Thus, the HPLC conditions used here allowed a fastanalysis of bilberry chemicals, making a higher sample throughput possible. Moreover, it is likelythat by adjusting the gradient and flow rate, the speed of analysis over the monolith column couldbe further improved without negative effect on compound separation.


Columns

Johanna WitzellSouthern Swedish Forest ResearchCentre, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden

Figure 2.HPLC chromatogram of non-hydrolysed samples of berries. 2 = delphinidin-3-glucoside; 3 = cyanidin-3-galactoside; 5 = cyanidin-3-glucoside; 15 = petunidin; 16 = peonidin; 17 = malvidin. Other peaks areunidentified compounds.

Reference:Nyman N.A. and Kumpulainen J.T., 2001: Determinationof Anthocyanidins in Berries and Red Wine by High-Performance Liquid Chromatography. J. Agric. FoodChem. 49: 4183-4187.


Analytical HPLCsection line 2section line 1

The efficiency of analytical methods is moreand more important in times of high costpressure. Fast chromatographic

separations and high sample throughput arefrequently the main goal of methoddevelopment.

One approach to achieve faster separations isto reduce the particle size of the silica used.However very small particles in HPLC columnsgenerate very high column backpressure and inmany cases (eg. with 1,7 μm particles) it isnecessary to use special HPLC systemsenabling pressures up to 1000 bar (15.000psi).

A better approach is to use Chromolith® HPLCcolumns, which enable ultra fastchromatography with standard HPLC systems.The recent publication by Bolivar and co-workers1) describes a time saving of 85% inone application using Chromolith® columns.Depending on the mode of calculation, thisconverts to a cost saving of 80.000 Euro peryear.

In contrast to conventional HPLC columns filledwith silica particles, monolithic HPLC columnsare made of one single piece of continuouslyporous silica with high mechanically stability.The combination of high permeability and highsurface area makes monolithic silica ideal forfast HPLC separations.2)

Figure 1 shows the structure of monolithicsilica under the electron microscope. It looksvery similar to the typical structure of coral. Thesilica forms a continuous skeletal structure andthe space around the silica is completely openand permeable. Liquids are able to flowthrough these open “macropores” with verylow resistance. The unique characteristic of HPLC columnsmade of monolithic silica, e.g. Chromolith®

HPLC columns, is that they provide optimalchromatographic performance withsignificantly lower backpressure thanconventional particulate HPLC columns.

Reduced requirement for sample preparation!A second important characteristic ofChromolith® HPLC columns is that they can beused with simpler sample preparationtechniques. Many samples contain tinyparticles in suspension, which cause blockingor plugging of conventional particulate HPLCcolumns. Such particles tend not to block themonolithic silica structure with its large 2 μmmacropores. Because of this, the columnlifetime is much longer. This opens the way tovery simple “dilute and shoot” samplepreparation techniques1,3,4). The sample issimply filtered through a 0,45 μm syringe filter,

Chromolith® HPLC columns convert standard HPLCsystems into robust racehorses

Figure 1.

Figure 2.

Monolithic silica

columns (Chromolith®)

enable very fast HPLC

analysis with high

detection sensitivity.

These columns are

ideal for use with

standard HPLC

systems.

The requirements for

sample preparation

are reduced, thereby

further reducing

analysis time and cost.



Columns

in some cases after dilution and centrifugation,and then injected directly onto the column withno further sample preparation. Even in theanalysis of foodstuffs or phytopharmaceutics,solid phase extraction steps can frequently beeliminated.With Chromolith® HPLC columns, time savingsresult not only from faster separations. Owingto the reduced requirement for samplepreparation, total analysis time and costs aredramatically reduced. This is demonstrated bythe following examples:

1) For the determination of caffeine in coffee3)

the time of analysis was reduced from 18minutes using particulate columns to only 0,68minutes using a Chromolith® SpeedRodcolumn. The sample throughput increased to 60samples per hour using a completely standardHPLC system. No solid phase extraction stepwas required. The samples were simply filteredbefore injection.

2) For the determination of iso-α-acids in beer1)

a cost saving of 60% and time savings of 86%has been reported when using a Chromolith®

Performance column . The RSD was improvedfrom 1,6% to 0,9%. The samples of light beerwere injected directly onto the column afterfiltration. A solid phase extraction step waseliminated (this step was required when usingparticulate columns).

Ultra fast separationswith Chromolith® 3mm

Chromolith® HPLC columns with 3 mm internaldiameter instead of 4,6 mm enable very fastseparations at relatively low flow rates usingisocratic (Figure 2.) or gradient conditions (Figure 3.). Figure 3. shows the separation of 8 sulphonamides in only 1,4 minutes at 2 mlmin-1 6). The same separation using 3 μmparticulate columns typically requires about 6 minutes.

References:1. A.Bolivar, M.Gasparri, C.Zufall,

J.Am.Soc.Brew.Chem. 2006,64(1),39-46 2. K.Cabrera, J.Sep.Sci, 2004, 27, 843-8523. Paraskevas D. Tzanavaras, Demetrius G. Themelis -

Analytica Chimica Acta (2006),doi:10.1016/j.aca.2006.07.081

4. V.Borges et al, J.Chromatography B, 2004, 804,277-287

5. Hydroxybiphenyls - a class of compounds used asfungicides and relevant for food andenvironmental analysis

6. LaChrom Elite Application Note 2006 -Sulphonamides

Figure 3.


Purospher® STAR HPLC columns – the best choice for successful HPLC separations

Purospher® STAR RP-18 endcappedHPLC columns make it very simple tochoose the best column for mostapplications, as proven by the manyusers who appreciate their excellentproperties. The absence of metals in the silicamatrix, in combination with a completecoverage of the silica surface, enablesthis stationary phase to producetailing-free chromatography of acidic,basic and chelating compounds.Purospher® STAR provides the bestall-round retention characteristics.The outstanding pH stability up to pH10,5 allows the separation of strongbasic compounds with alkaline eluents.In addition, Purospher® STAR RP-18endcapped is suitable for use with100% aqueous mobile phases.The combination of thesecharacteristics makes Purospher® STARRP-18 endcapped an all-round topperformance column, almost universalin its range of applications. Experiencethe performance of Purospher® STARRP-18 endcapped HPLC columns – onecolumn for different demands.Purospher® STAR RP-18 endcapped isideally suited to the separation of, forexample, Aflatoxins in foodstuffs withvery high sensitivity.

Aflatoxins in foodstuffsAflatoxins B1, B2, G1 and G2 are the main toxins produced by Aspergillus flavus, A. parasiticus andA. nomius. These toxins are of relatively high molecular weight and contain one or moreoxygenated alicyclic rings. They can contaminate food products when storage conditions arefavourable to fungal growth. The main aflatoxin contaminants have been reported in maize,peanuts, brazil or pistachio nuts, copra and cotton seeds. Aflatoxins are carcinogenic, mutagenic,teratogenic and immunosuppressive to most animal species. The Internal Agency for Research onCancer (IARC) has classified all four aflatoxins as group one carcinogens. Confirmation of the presence of aflatoxins in a sample by HPLC requires derivatisation of theaflatoxins B1 and G1 in order to enhance their natural fluorescence under UV light and make themmore easily detected. Previously, the only options available for derivatising aflatoxins involved theuse of either trifluoroacetic acid (TFA) or iodine. Both of these methods are reliable, however theydo have some significant limitations, which can be overcome by use of a third method with theCoring Cell.

The European standard HPLC method EN 12955 for the determination of aflatoxin B1 and the sumof aflatoxin B1, B2, G1 and G2 in cereals, shell-fruits and derived products features a high-sensitivefluorescence detection by post column derivatisation with iodine and immunoaffinity column cleanup. Iodine derivatisation as a post column technique does have other disadvantages such as longerreaction time at higher temperatures (peak broadening), additional HPLC pump necessary anddaily preparation of the corrosive iodine solution. This European standard specifies a method forthe determination of aflatoxin content of greater than 8 μg/kg.

The official German method for measuring compounds in food products (§35 LMBG methods)features the clean-up of the aflatoxins B1, B2, G1 and G2 by means of an immunoaffinity columnand the following HPLC detection with an electrochemical cell (Coring Cell). The Coring Cell is anelectrochemical cell which generates the derivatising agent, bromine, from potassium bromidepresent in the mobile phase. The derivatisation of aflatoxins occurs rapidly (reaction time isapproximately 4 seconds) at ambient temperature. A daily preparation of derivatising reagent(iodine) is not necessary and the additional pump for addition of derivatising reagent is notneeded.

For both methods Purospher® STAR RP-18 endcapped shows excellent separation of the 4aflatoxins with outstanding peak symmetry as the comparison of different HPLC columnsdemonstrates (Figure 1- Figure 3).

Analytical HPLC

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

0

20

60

40

80

100

Retention Time (min)

G2

G1

B2

B1

Chromatographic conditions:Column: EcoCART® 125-3 LiChrospher RP-18, 5 μmMobile phase: Water/Methanol/Acetonitrile (65:15:20)+ 100 μl HNO3 65%/l + 119 mg KBr/l

Flow rate: 0,6 ml min-1

Injection volume: 100 μl

Derivatisation: Coring cell

Figure 1.Separation of Aflatoxins on LiChrospher® RP-18

59

0 2 4 6 8 10 12 14 16

58,5

58

57,5

57

56,5

56

55,5

55

54,5

B1

B2

G1

G2

Chromatographic conditions:Column: 250-4.6 RP-18 column, 5 μmMobile phase: Water/Methanol/Acetonitrile (6:3:2)+ 350 μl HNO3=4 mol/l + 120 mg KBr/lFlow rate: 1 ml min-1

Injection volume: 200 μl


Figure 2.Separation of Aflatoxins on RP-18 column ;Chromatogram from the § 35 LMBG method

0 2 4 6 8 10 12 1614 18 20-5

0

5

10

15

20

25

30

35

-5

0

5

10

15

20

25

30

35

Minutes

FLU

Time: 19,9796 Minutes - Amplitude: -0,40675 FLU

G2

G1

B2

B1

Chromatographic conditions:Column: Purospher® STAR RP-18 endcapped, 150 x 4,6 mm, 5 μm Pre column: Purospher® STAR RP-18 endcapped, 4 x 4 mm, 5 μm Mobile phase: Water + 183,1 mg KBr/l + 154 μlHNO3 65%/l / Methanol / Acetonitrile65 % A / 17.5 % B / 17.5 % C(V/V/V), IsocraticFlow Rate: 1 ml min-1

Temperature: 40 ºCInjection volume: 100 μl


Figure 3.Separation of Aflatoxins on Purospher® STARRP-18 endcapped



Columns

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

-0,25

0,00

0,25

0,50

0,75

1,00

1,25

1,50

-0,25

0,00

0,25

0,50

0,75

1,00

1,25

1,50

Minutes

FLU


G2

G1

B2B1

Method 1: Aflatoxins with iodine derivatisation (EN 12955)

G2 - 30 pg/ml, G1 - 100 pg/ml, B2 - 30 pg/ml, B1 - 100 pg/ml

Chromatographic conditions:Column: Purospher® STAR RP-18 endcapped, 150 x 4,6 mm,

5 μm Pre-column: Purospher® STAR RP-18 endcapped, 4 x 4 mm, 5 μm Mobile phase A: Water/Acetonitrile/Methanol 65/17,5/17,5 (V/V/V)Mobile phase B: Rinse the system after the sequence for 30 minutes with

90/10 water/Methanol (V/V)Flow Rate: 1 ml/minDetection: Fluorescence EX 365 / EM 435Temperature Oven: 60 ºCInjection volume: 50 μl

Post column derivatisation:Derivatisation reagent: saturated iodine solutionReaction pump: The pump is stopped by the timer

of the L-2130 pump when the rinsing program is started. Never let this pump run when the L-2130 pump is stopped. The iodine will damage your column.

Derivatisation coil: PTFE knitted coil, 5 m x 0,5 mm I.D. or home made PEEK coil 3 m x 0,5 mm I.D.

Flow Reaction pump: 0,2 ml/min

0 2 4 6 8 10 12 1614 18 20

-0,5

0,0

0,5

1,0

1,5

2,0

2,5

-0,5

0,0

0,5

1,0

1,5

2,0

2,5

Minutes

FLU


G2

G1

B2

B1

Method 2: Aflatoxins with coring cell (§ 35 LMBG)

B1 and G1: 10 pg/mL; B2 and G2: 2.5 pg/mL

Chromatographic conditions:Column Purospher® STAR RP-18 endcapped, 150 x 4,6 mm,

5 μm Pre-column Purospher ® STAR RP-18 endcapped, 4 x 4 mm, 5 μm Mobile phase: Water + 183,1 mg KBr/l + 154 μl HNO3 65%/l /

Methanol / Acetonitrile65 % A / 17,5 % B / 17,5 % C(V/V/V), Isocratic

Flow Rate: 1 ml min-1

Detection: Fluorescence EX 365 / EM 435Temperature: 40 ºCInjection volume: 100 μl

Post column derivatisation:Derivatisation coil: PEEK coil, 1,38 m x 0,25 mm I.D.

Full applications are available from your localVWR sales office

>

>Technical DataMaterial: Cross-linked polystyrene-co-divinylbenzene polymer

Particle size: 5 μm

Pore size: 100 Å

Ordering Information

0.0

2.0x10 6

4.0x10 6

6.0x10 6

8.0x10 6

1.0x10 7

1.2x10 7

1.4x10 7

20%

40%

60%

80%

100%

20 30 40 50 60 70 80 90

Data courtesy of S. Cohen and C. RitlandNevada Department of Agriculture

Column Bleed Characteristics PRP-h1 vs. Traditional Polymeric Column

Time [min]

MS

Sig

nal

[m

/z =

50-

2000

]

% A

ce

ton

itri

le

0.0

2.0x10 6

4.0x10 6

6.0x10 6

8.0x10 6

1.0x10 7

1.2x10 7

1.4x10 7

20%

40%

60%

80%

100%

20 30 40 50 60 70 80 90


0,0

2,0x10 6

4,0x10 6

6,0x10 6

8,0x10 6

1,0x10 7

1,2x10 7

1,4x10 7

20%

40%

60%

80%

100%

20 30 40 50 60 70 80 90


Column Bleed Characteristics PRP-h1 vs. Traditional Polymeric Column

Time [min]

MS

Sig

nal

[m

/z =

50-

2000

]

% A

ce

ton

itri

le

50 mm 100 mm 150 mm 250 mm

2,1 mm ID 554-1378 554-13814,6 mm ID 554-1377 554-1379 554-1382 554-138410 mm ID 554-1380 554-1383100 mm ID 554-1385

PRP-h1 Guard ColumnsAnalytical Guard Column Starter Kit (1 holder, 2 cartridges) 554-1386Analytical Replacement Cartridges (5/pk) 554-1387Semiprep/Prep Guard Column Starter Kit (1 holder, 2 cartridges) 554-1388Semiprep/Prep Replacement Cartridges (2/pk) 554-1389

PRP-h1 Bulk Resin12-20 μm Bulk Resin (1 g) 554-1390


HAMILTON’s new PRP-h1 HPLC-columns:higher sensitivity, half backpressure and no column bleed

HAMILTON´s newly developedHPLC column phase based on PS-DVB retains the advantagesover silica usually associated withpolymeric columns and at thesame time minimises columnbleed.

In fact the PRP-h1 columns havevirtually no bleed, even at 100%acetonitrile and run at half thebackpressure of traditionalpolymeric columns.

The outstanding pH stability(pH 0-14) completes theexceptional features of these newcolumns.

Analytical HPLC Columns

Determination of Naproxen and IbuprofenColumn: PRP-h1, 5 μm, 100 Å, 4,1 x 50 mm

Mobile Phase:A: 10 mmol/l Sodiumdihydrogenphosphate, pH=2B: Acetonitrile, Isocratic 50% A / 50% B

Flow Rate: 0,6 ml/min

Temperature: 60 °C

Detection: UV@ 230 nm

Sample: (1) Naproxen (110 μg/ml)(2) Ibuprofen (830 μg/ml)

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10

1

2

mA

U

Time [min]

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10

1

2

mA

U

0

20

40

60

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100

120

140

160

0 1 2 3 4 5 6 7 8 9 10

1

2

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10

1

2

mA

U

Time [min]

>


When operating according to

quality management systems (e.g.

according to GMP, GLP or ISO

guidelines), validation of the

procedures used is of the utmost

importance. However, this process

including the compilation of the

validation report is often tedious

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Validation Manager is a valuable,

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and automatically produces the

validation report you need.

• Universally acceptedValidation Manager is based on theinternational guidelines for validation ofanalytical methods as established by EP, USP,FDA, ICH and ISO.

• Versatile configuration for allanalytical techniquesThe validation procedure can be configuredwith regard to formats, used terms andlanguage as well as with regard to calculationmethods and statistical tests to be applied.Several pre-configured templates can beselected. New templates can be created andstored. This high degree of versatility enablesthe Validation Manager to be used for thevalidation of practically all analyticalprocedures.

• Easy operationValidation Manager is extremely simple to use.Only a few questions must be answered in awizard dialogue, then the validation projectand document is created at a push of a button.

• Easy creation of samplepreparation worksheets andinjection tablesAnother programming assistant helps to createsample preparation worksheets for thedifferent method characteristics to be assessed.In addition even injection tables for the

EZChrom EliteTM chromatography data systemcan be created automatically.

• Easy data entryAfter data acquisition chromatography datacan be imported from the chromatographydata systems EZChrom EliteTM , D-7000 HPLCSystem Manager and Agilent ChemStation®.Moreover, any data can be copied fromEXCEL™ tables or inputed manually.

• Automatic calculationAfter data entry the required methodcharacteristics are automatically calculated andstatistically checked according to theconfigured procedures.

• Automatic Report CompilationAfter entering your comments and decisions,the entire method validation report is created -with only a mouse click.

• Validated and fully FDA 21CFRpart 11 compliantValidation Manager software is validated andthe validation certificate is included in thesoftware package. It contains all functions tomake it fully FDA compliant.

• Substantially time-savingAs a comprehensive tool for computer-assistedmethod validation Validation Manager supportsthe user in the different steps of the validationprocedure. Substantial time savings can berealised especially by the automaticcompilation of the validation report.

Validation Manager - the time and costsaving tool for validation of analyticalmethods can save days or even weeks of work.

If you have the need to validateanalytical methods please contactour specialists and learn moreabout Validation Managersoftware.

Validation ManagerThe quick and reliable way to validateanalytical methods


Software


• Ease-of-use

• Instrument control

• Data processing

• Regulatorycompliance

• Networkscaleability

>

Analytical HPLC

EZChrom EliteTM SoftwareA uniform and scaleable Software Solution

Example of awizard for fast

access to themost common

functions

Control of the LaChrom Elite® system: pump setup screen

Display of instrument status


EZChrom EliteTM is one of the most popular chromatography data systems in the world. It supportstoday’s demands in the analytical field - from a single workstation up to a fully equipped andcompany-wide client/server system. EZChrom EliteTM is a user-friendly, intuitive software, developedfor the current requirements of the pharmaceutical and chemical industry. It includes special toolsfor compliance with regulatory demands, e.g. FDA 21CFR11 and instrument andsystem validation.EZChrom EliteTM offers one uniform software platform both for digital control anddata processing and management of a large number of different chromatographysystems (HPLC and GC), including VWR-Hitachi LaChrom® and LaChrom Elite®

HPLC systems as well as those of most established suppliers in the HPLC- andGC market (for a complete list of supported instruments seewww.vwr.com). The uniform operation of the softwarefor different chromatography systems increasesefficiency and helps to reduce user training costs.EZChrom EliteTM offers a wide variety of functionsin chromatography system control, setup ofmethods and sequences, dataprocessing and visualisation as wellas compilation of custom reports.


Software

The next training on this great systemLocation: Darmstadt - GermanyHeld in English

Go to www.vwr.com/chromjournalto download a registration form

Basic Operator Training (BOT) 5-6 February 2008Training contents:This training addresses newcomers tothe EZChrom EliteTM Software, andcovers the following items:• Developing methods for data

acquisition and data processing• Setting-up and running sequences

for data acquisition and data (Re-)processing

• Working with chromatograms• Calibration and quantitation• Report generation with methods

and sequences• Advanced method options and

specialties

Add-on Training (DAD)7 February 2008Training contents:This training is an extension of the BOTfor users who are running a DAD (DiodeArray Detector). The contents of the BOT is required andassumed. The DAD-training day covers:• Specialities of the method setup

for DAD data acquisition and dataprocessing

• Working with 3-D and 2-D- data.Similarities and differences to 2-Ddetectors

• Creations and usage of spectralibraries

• Setting-up and using peak purityfeatures

• Specialities of DAD data basedreport generations

A charge will be made for training; pleasecontact your local VWR sales office for moredetails.

Rapid separationoptimisation with the NavigatorSystem


Your Navigator for optimal HPLC methodsFaster, sharper peak resolution achieved rapidly,intelligently and reliably.

The Navigator System from VWR Internationalis the first intelligent HPLC System that guidesyou safely through the difficulties of HPLCmethod development. It is so simple - justplace your sample in the autosampler, select acolumn, an eluent and then press the startbutton!

What can the Navigator Systemdo?First of all, the system carries out a gradientrun in order to obtain information about thepeaks and the corresponding analyte retentiontimes. Based on these data and using itsunique and sophisticated optimisationfunctions, the Navigator System then calculatesthe optimal linear and step gradients. With thehelp of a computer, it takes into account moremethod development possibilities than couldever be achieved on a manual basis. It candevelop a suitable separation method in lessthan 3 hours. In standard applications, such anoptimised method can normally subsequentlybe taken on as a routine analytical method.Even in extremely complex separations, theNavigator System can produce a basic methodrequiring only fine tuning.

With which HPLC applicationscan the Navigator System beused?The Navigator System was developed foruniversal use. It can be applied to theoptimisation of chemical and biochemical

analyses at universities, to methoddevelopment in the chemical andpharmaceutical industries and to foodstuffmonitoring.

What is the Navigator Systemmade up of?The Navigator System comprises a pre-configured combination of the high-performance HPLC system LaChrom Elite®

equipped with a special version of theacknowledged best automatic methoddevelopment software ChromSword® Auto. Allof this is available at a very attractive price.

What else can the NavigatorSystem offer?With easy-to-fit upgrades, the NavigatorSystem provides the following furtherpossibilities for professional HPLC methoddevelopment: The system can be adapted forautomatic column and solvent screening,whereby the optimised HPLC separationmethod is presented, even for highly complexapplications.

Apart from its special function for rapid HPLCmethod development, the Navigator System iscompletely equipped for professional routineanalytical work, e.g. for the QC of chemical,pharmaceutical and food products as well asfor environmental analysis. With its high-performance specifications, its highsample capacity, its highly sensitive diode array

Analytical HPLC

Automatically optimised step gradient in the separation of 7 sulphonamides.Column: LiChrospher® 60 RP-select B, 125-4 mmEluent: Water / acetonitrileFlow rate: 1,5 ml/min

Rapid separation optimisation of a pharmaceutical sampleTop: Optimised linear gradientBottom: Optimised step gradientColumn: Chromolith® SpeedROD RP18e

50-4,6 mmEluent: Water / acetonitrile Flow rate: 1,5 ml/min

The Navigator System comprises the following modules:• L-2130 pump with low-pressure gradient accessory and solvent degasser

• L-2200 Autosampler

• L-2300 Column oven

• L-2455 Diode array detector with USB facility

• Organiser

• EZChrom EliteTM Chromatography Data System

• Navigator method development software

• PC with monitor and keyboard



HPLC system

Rapid separation analysis of a pharmaceutical sample: Optimised step gradientColumn: XTerra® RP18 3,5 μm 4,6-100 mmEluent: Water / acetonitrileFlow rate: 1 ml/min

N

S

EW

NW NE

SESW

Rapid separation optimisation of the ingredients of apple juiceTop: Optimised linear gradientBottom: Optimised step gradientColumn: Chromolith® Performance RP18e

100-4,6 mmEluent: Water / acetonitrileFlow rate: 1,5 ml/min

detection system, its manifold automation andqualification functions and its absolutereliability, the LaChrom Elite® System is alsoideal for routine analytical work.

Discover the benefits of the Navigator System;it will definitely increase the productivity ofyour HPLC lab.

It will automatically find the most suitableseparation method with the shortest analysistime.

Why not let one of ourspecialists demonstratethe benefits of theNavigator-System in yourown lab? Contact your local VWRsales office

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The new inert L-2130i pump for biochromatography and cation chromatography

The new biocompatible, multi-purpose, chemically inert L-2130i pump for bio and ionchromatography meets the highest demands for detection sensitivity, base-line stability andreliability.

When the L-2130i pump was developed, the manufacturers consciously decided against the use ofmetallic material. Titanium was also avoided as this contains impurities from many other metalsand dissolves in various solvents, e.g. in mixtures of methanol and water. Instead, inert syntheticmaterials—pressure stable PEEK (poly-ether-ether-ketone) in particular—and ceramic materialswere used to meet the demand for biocompatibility, inertness and non-metallic content.

The L-2130i is a serial dual plunger pump with the extra advantage of superior flow stability, lowpulsation, and exceptional reliability and operating safety.

The pump's electronic control system compensates for the solvent's compressibility and eliminatesany outstanding pulsation. This compensation and the exceptionally high flow stability leads toextremely stable analyte retention times.

The innovative PASS function (Pump Autosampler Synchronisation) increases the precision ofretention times considerably when working with gradients.

A built-in plunger rinsing mechanism that can be applied automatically in conjunction with anautosampler, effectively reduces the wear on the plunger and the pump seal through bufferdeposition.

The L-2130i pump can be upgraded to a quaternary gradient pump that can then be used acrossthe entire flow range up to 10 ml/min.

The pressure range from up to 275 bar enables the pump to be used for all applications includingbiochromatographic and ionchromatographic polymeric-phase separations as well as for the

analytical HPLC RP-phases for peptide analysis, for example.

The inert L-2130i pump has a wide range of system controlfunctions, which are especially beneficial for biochromatographicapplications where gradient elution is required, or where valvesmust be activated, an additional pump controlled or otherequipment connected. There are four time-controlled switchcontacts, as well as other contact outputs for Start, Stop and Busy.In this way, the pump can be used as a master controller for thechromatography system when operated without control software.

In most applications, however, the L-2130i pump is integrated into theLaChrom Elite® system by E-line communication® and completely

controlled by the EZChrom Elite® software.

In conjunction with EZChrom Elite® data acquisition and control software, there is a completerange of GXP functions available, such as recording pressure characteristics during analysis,displaying serial numbers, a detailed log book and audit trails, and documenting pump settingsand solvent consumption since the seal was last changed.

The inert L-2130i pump meets the high Hitachi quality standards and is supplied with a test reportand quality certificate.

Analytical HPLC Pump

• Non-metallic,chemically inert (PEEK, ceramic)

• Exceptionally high flow stability

• Exact gradientformation

• High pressure stability

• Front access for allreplacement parts

• Small footprint


The new, highly sensitive CD 5 conductivitydetector for ion chromatography meets thehighest demands for detection sensitivity,base-line stability and reliability.

It is equipped with a multiple-electrodemeasuring system, which ensures a stable baseline. The measuring cell, which isthermostatable at many levels, can be placedoutside the detector or in a column thermostat.Greatly improved stability of the analysissystem can be achieved by active cellthermostatisation and/or columnthermostatisation, allowing highly sensitivemeasurements in the range of 0 to 1000 S/cm-1.

The measuring system remains unaffected bythe ambient temperature and stabilisation ofthe base line can therefore take place within arelatively short amount of time. An autozerofunction allows the suppression of the baseconductivity of the eluents. An additional offsetfunction, different sensitivity settings and thepolarity reversing of the detector signal offerthe user greater flexibility when analysinganions and cations, particularly in the sensitiveborder area.

All data is inputted via a membrane keypad.The large LCD ensures that conductivity isclearly displayed. Once the device has beenswitched on, the base line becomes stablewithin approx. half an hour and the detector isthen ready to carry out the measurements.A series of different input and output contactsmakes it possible to communicate with otherHPLC devices, such as an autozero and markerinput and outputs for recorders andintegrators.

The CD 5 conductivity detector can beintegrated seamlessly into the LaChrom Elite®

system thanks to its space-savingconstruction® and design® and, together withthe VWR Hitachi HPLC modules, offers ahigh-performance ion chromatographysystem for research and routine.

A new conductivity detector with excellent properties


Conductivitydetector

• Exceptional sensitivity

• High base-line stability

• Low base-line noise

• Thermostablemeasuring cell

• Small footprint

Chromatogram A:determination of anions

with a sensitivity of 50 ppb per anion

Chromatogram B:determination of anions

in LiChrosolv water

Chromatogram C:determination of anions

in Milli-Q water

Analytical Conditions• Samples:

50 ppb standard,LiChrosolv® and Milli-Qwater

• Separating columns:Shodex IC NI-424

• Mobile Phase:1,7 mM phthalic acid 300 mM boric acid

• Detector:CD 5, 10 μS/cm range


LaChrom Elite® HPLC system now also controlled by the Chromeleon® Chromatography Data System The well-proven LaChrom Elite®

HPLC system from VWR/Hitachi

now becomes even more versatile:

now it can be controlled by the

Chromeleon® software from

Dionex as well.

Analytical HPLC Software

Laboratories throughout the world use the LaChrom Elite® systems from VWR/Hitachi and manyusers prefer VWR/Hitachi LaChrom Elite® LC instruments for their robust and versatile operation.This HPLC instrument line features excellent precision and sensitivity as well as minimum samplecarry-over.

The new Instrument Control Software for the LaChrom Elite® system is an add-on softwarepackage for the Dionex Chromeleon® Chromatography Data System 6.8. This add-on packageallows you to fully control the LaChrom Elite® system within the Chromeleon® Data System. TheHitachi LaChrom Elite® system runs along with instruments from other manufacturers in onesoftware platform. This standardises your operations with a single software interface to manageyour data sets, instrument parameters, sequences, methods and reports.

You benefit from one single cost-effective solution to support your analytical work, thereforeminimising software-training requirements and simplifying the exchange of technical staff.

The Hitachi Control for Chromeleon® was developed by Hitachi engineers in cooperation withDionex developers using Dionex software development tools.

Flexible Control: Whether your Chromeleon® implementation is a single workstation or afully equipped and company-wide enterprise solution (Client/Servernetwork) the new LaChrom Elite® control can be utilised for bothdeployment types. The Instrument Control Software for Hitachi LaChromElite® provides full control for pump, autosampler, oven and detectors. Pump control: All gradient information, flow, auxiliary events and a timetable tospecify flow and gradient composition as a function of time.Oven control: Settings include temperature and tolerance setup.Detector control: UV, UV/VIS and Fluorescence detectors can be setup in control softwareto specify wavelength, autozero controls, and lamp status as well as atimetable to specify desired wavelengths and other parameters as afunction of time.

Users can see the Instrument Status screen which shows all informationin a customisable panel.

Regulatory Compliance: All LaChrom Elite® modules contain a maintenance log, which records the use of replacementparts. They also have in-built diagnostics, which allow the operational conditions of the system tobe monitored.

When used in conjunction with the Chromeleon® software, the complete range of GXP-relatedfunctions and 21 CFR Part 11 compliance is achieved e.g. detector lamp usage and replacements,audit trails, automatic documentation of the instrument parameters, maintenance parameters, etc.

Figure 1: LaChrom Elite Controlfor the Dionex Chromeleon®

Software. The panel makes it easyto follow up the adjustments andchromatograms

Short overview of modulefunctions and status with allparameters


To maintain the high quality of

their solvents until you use them,

Merck have developed a practical

packaging concept for chemicals.

For LiChrosolv®, Perpsolv® (HPLC), Uvasol® (UV/VIS), SeccoSolv®, SupraSolv® and Unisolv® (GC) we offer a unique variety of differentpackaging sizes and types:• Glass bottles• Aluminium bottles• Stainless steel barrels• Other barrels and containers• Withdrawal systems

Ideal packaging and withdrawal systems for HPLC solvents


Solvents

BDH Prolabo chemicals The new Chemical Brand from VWR International

New HiPerSolvChromanormrange

• High purity solvents for HPLC applications

• Guaranteedspecification conformsto HiPerSolv solvents

• High performance andreliability

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Combining the best qualityand price competitivecharacteristics

For a free copy of the new BDHProlabo Chemicals

brochure or furtherinformation, please

contact your local VWRInternationalorganisation or checkout vwr.com

Your benefits:

• Easy, safe and contamination freesolvent handling

• Central storage and supply possible• Individual user installation or other

customised solutions possible• Direct connection to laboratory

equipment such as an HPLC isachievable

Merck’s expertise is recognised in theirauthorisation as an official inspection authorityby the Federal Institute for Material Researchand Testing of Germany (RAM).

Examples of use:

• Feeding solvents (eluents) to HPLC machines

• Disposal of waste media

• Use in all self-contained systems entailing sterile transfer of culture mediaunder pressure (e.g. fermenter)


Schott Duran® HPLC laboratory bottles

These bottles facilitate the safe transfer of liquid media

within an enclosed, sterile system.

Ideal for chemical and biotechnology laboratories.

This is how it works

The SCHOTT DURAN® HPLC bottle is a complete system. It is made up of the special SCHOTTDURAN® pressure plus laboratory bottle and a screw cap with four ports. The four ports make itpossible to serve three stations at the same time from one container. At the same time, the fourthport can be used for sterile pressure equalisation. The system is suitable for tube diameters of 1,6mm (1/16 inch) and 3,2 mm (1/8 inch).

Ports that are not in use can be closed with a silicone seal.

The SCHOTT DURAN® pressure plus bottle is pressure resistant thanks to its special geometry. Thepressure resistance is TÜV-GS certified (TÜV ID: 0000020716 ) and safety tested to EN 1596. Thebottle has a retrace code for downloading a certificate of conformity from the Internet.This contains not only the batch and manufacturing data but also additional information aboutstandards and USP/FDA conformity.

Your advantages

• Hoses may be fixed tightly to prevent them from moving and the HPLC column from running dry• The bottle has a special geometry according to ISO 4796, and is pressure/vacuum resistant in a

range from –1 to +1,5 bar • The cap is made of PTFE and PP and can be completely autoclaved together with the bottle

without taking it apart• Can be repeatedly reused• The optional silicone seals can also be used as septum for subsequent addition of additives• Working in the enclosed system retains toxic gases in the bottle• The system is suitable for universal use because the screw cap is based on the standardised ISO

GL 45 thread • Practical complete system: the SCHOTT DURAN® HPLC laboratory bottle including 4-port screw

cap is ready for immediate use

Solvents Bottles


Until recently, subjecting a GCcolumn to crude plant extractswould be a sure way to shortenthe longevity of the column as theextracts are notorious fordamaging column phase.However, to test the robust phasechemistries of the new Forterange of GC columns we haveactively subjected the columns tothe “Green Tea Test”.

In the green tea experiment, a standard testmix was analysed to produce a controlchromatogram. Then the column was subjectedto 100 injections of green tea followed by arepeat analysis of the test mixture. Theresulting before and after chromatogramsshow an 'as new' performance, demonstratingthe lack of activity and inert nature of thehighly robust column phase (Figure 1)

Pushing the concept further, the green tea testwas carried out on more polar phases such asthe BPX50. Traditionally, polar phases are lessrobust than non-polar phases. Despite this, theBPX50 handled the green tea test and evenafter 200 injections of green tea there waslittle difference between before and afterchromatograms. The analysis of organochlorinepesticides shows that the highly robust phaseremained inert, as evident by the lack of on-column decomposition of any of thepesticides (Figure 2)

Similar robustness can be seen with the BPX35,which provided excellent separation of PAHs after100 injections of the dreaded green tea (Figure 3)

Extreme column abuse like the green tea testmight seem drastic but, in a busy laboratory,time restraints and cost concerns can oftenlead to compromises in sample clean-up.Conventional wisdom would have it thatinadequate sample preparation is a trade offwith reduced column life, ultimately leading tomore frequent column replacement andinstrument downtime. However, even if yoursamples are not as dirty as green tea, the newForte range of columns will provide exceptionalcolumn durability and performance.

Taking the science a little further, weinvestigated absolute bleed measurements forthe Forte columns. With clean samples, columnlife is determined by gradual loss of phase(resulting in deteriorating resolution) that takesplace as a result of column bleed. Columnbleed is simply phase leaving the column and,by quantifying bleed against a standard, therate of phase loss can be measured inng/second. Since the initial amount of phase inthe column is known, the time taken for 1% ofthe phase to be lost can be calculated. Theabsolute bleed results can be seen in (Figure 4)

Not satisfied with subjecting our columns togreen tea, we decided to test the widely heldbelief that exposing a column to oxygen orrunning out of carrier gas, particularly atelevated temperatures (especially with polarphases), will quickly damage the column.Contrary to this belief, even the more polarphases of the Forte range coped with this typeof abuse. Figure 5 shows that after a briefre-conditioning the performance of the BPX50was restored after no carrier gas for 90 minutesat 250 °C. Even using 1% air mixture as acarrier gas caused little damage as shown byanalysing organochlorine 8081 mix (Figure 6)

Although not wanting to encourage poorsample preparation and extended columnabuse, our experiments have demonstrated thedurability of the Forte range. More columnabuse to follow!

SGE’s new Forte column gets tough with green tea

by Dr. Hugh Malkin, Market Development Specialist


Gas Chromatography


NEW AcroSep™ Chromatography Columnsfor Ion Exchange from Pall Life Sciences

BioChromatography

Pall has recently extended their range ofchromatography products to include the newAcroSep™ Chromatography Columns for IonExchange. The columns are ideal foroptimisation studies of protein purificationschemes using small sample volumes prior toscale-up and offer an efficient and reliablemethod for sample preparation of proteins forstructural, functional and yield analysis.

The ion exchange columns contain Pall’sHyperD chromatography sorbents, which arecreated using patented “gel-in-a-shell”technology.

Gel-in-a-Shell Design

The gel-in-a-shell bead is constructed of ahigh-capacity hydrogel polymerised within thegigapores of a rigid ceramic bead. The capturechemistry is attached to the hydrogel withinthe bead pores. The advantages of thisstructure are many, including higher than usualcapacity, tolerance to fast chromatographyruns without a concomitant increase inbackpressure, and greater salt tolerance for ionexchangers.

Whether your application

challenges are in research,

scale-up or polishing, Pall offers

an extensive portfolio of bulk

resin chromatography media

for affinity, ion exchange,

size exclusion, hydrophobic

interaction and hydroxyapitite

chromatography.

For a complete listing ofPall’s Protein Purificationproduct range or toorder a new ProteinSample Preparation andAnalysis ApplicationManual please contactyour local VWR salesoffice.

Bulk chromatography sorbents (bottled in 1 to1000 ml quantities) are also available fromPall. The chromatography sorbents can be usedin traditional chromatography columns or, forquick separations, can be used in centrifugalspin columns (e.g., Pall Nanosep® devices). Forhigh throughput separations or scoutingassays, the sorbents can be combined with theAcroPrep™ filter plates with up to 96 mini-columns that can be simultaneously processed.

Pall also offers chromatography-based kits forabundant protein removal. These ready-to-usekits include all components necessary fordepletion or selection of albumin and/or IgGfrom multiple species.

To assist with your Proteomics research Pall has developed a detailed application handbookcontaining a range of protocols for samplepreparation and analysis. The handbookcontains protocols for handling thechromatography sorbents and the use of Pall'sother proteomic products.


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Columns

Features:-

Gel-in-a-Shell Technology –Patented HyperD ion exchangechromatography resin is a highcapacity hydrogel polymerisedwithin the large pores of a rigidceramic bead

Rapid and Efficient – Rigidresin structure allows faster flowrates and higher pressures whilemaintaining high dynamic bindingcapacities.

High Resolution – Distinctseparation of proteins forcomplete isolation andpurification

High Recovery – Maximumrecovery yielding highly pureproteins

Versatile – Luer lock inlet andoutlet for convenient use withsyringe, pump or automatedchromatography system

Convenience – Hexagonalcollar, prevents column fromrolling when set down

User-friendly – Colour-codedand labelled by chemistry type

Description Qty/Pk Cat. No.

HyperD F CM 1ml Column 5 569-1000HyperD F S 1ml Column 5 569-1001HyperD F Q 1ml Column 5 569-1002HyperD F DEAE 1ml Column 5 569-1003

Ion Exchange ResinParticle Stability Cleaning Ion Exchange

Media Function Size (μm average) Range pH pH Capacity (1)

CM Ceramic Weak Cation 50 2-12 1-14 > 60 mg/ml (2)HyperD® F Exchanger

DEAE Ceramic Weak Anion 50 2-12 1-14 > 85 mg/ml (3)HyperD F Exchanger

Q Ceramic Strong Anion 50 2-12 1-14 > 85 mg/ml (3)HyperD F Exchanger

S Ceramic Strong Cation 50 2-12 1-14 > 75 mg/ml (3)HyperD F Exchanger

(1) Dynamic binding capacity determined at 10% breakthrough, 200 cm/h with7 ml resin packed in a column of 1,1cm ID and 7 cm height using:

(2) 5 mg/ml human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4,7(3) 5 mg/ml BSA in 50 mM Tris-HCl buffer, pH 8,6(4) 5 mg/ml lysozyme in 50 mM sodium acetate buffer, pH 4,5

AustriaVWR International GmbHGraumanngasse 71150 WienTel.: 01 97 002 0Fax: 01 97 002 600E-mail: [email protected]

BelgiumVWR International bvba/sprlHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenTel.: 016 385 011Fax: 016 385 385E-mail: [email protected]

DenmarkVWR International ApSValhøjs Alle 174-1762610 RødovreTel.: 43 86 87 88Fax: 43 86 87 90E-mail: [email protected]

FinlandVWR International OyPihatörmä 1 C 102240 EspooTel.: 09 80 45 51Fax: 09 80 45 52 00E-mail: [email protected]

FranceVWR International S.A.S.Le Périgares – Bâtiment B201, rue Carnot94126 Fontenay-sous-Bois cedexTel.: 0 825 02 30 30 (0,15 EUR TTC/min)

Fax: 0 825 02 30 35 (0,15 EUR TTC/min)

E-mail: [email protected]

GermanyVWR International GmbHHilpertstrasse 20aD - 64295 DarmstadtTel.: 0180 570 20 00*Fax: 0180 570 22 22*E-mail: [email protected]*14 Cent/Minute aus d. dt. Festnetz

IrelandAGB Scientific LimitedA VWR International CompanyOrion Business CampusNorthwest Business ParkBallycoolin, Dublin 15.Tel.: 01 8822222Fax: 01 8822333E-mail: [email protected]

ItalyVWR International s.r.l.Via Stephenson 9420157 Milano (MI)Tel.: 02 332 03 11Fax: 800 152 999E-mail: [email protected]

The NetherlandsVWR International B.V.Postbus 81981005 AD AmsterdamTel.: 020 4808 400Fax: 020 4808 480E-mail: [email protected]

Northern IrelandAGB Scientific LimitedA VWR International CompanyA10 Harbour Court, 7 Heron Rd.Sydenham Business ParkBelfast, BT3 9HBTel.: 028 9058 5800Fax: 028 9080 7812E-mail: [email protected]

NorwayVWR International ASKakkelovnskroken 1P.B. 45, Kalbakken 0901 OsloTel.: 0 2290Fax: 22 90 00 40E-mail: [email protected]

PortugalVWR International - Material deLaboratório, LdaEdifício NeoparkRua Tomás Ribeiro, 43- 3 D2790-221 CarnaxideTel: 21 3600 770Fax: 21 3600 798/9E-mail: [email protected]

SpainVWR International Eurolab S.L.Apartado 4808100 Mollet del Vallés - BarcelonaTel.: 902 222 897Fax: 902 430 657E-mail: [email protected]

SwedenVWR International ABFagerstagatan 18a163 94 StockholmTel.: 08 621 34 00Fax: 08 621 34 66E-mail: [email protected]

SwitzerlandVWR International AGLerzenstrasse 16/18 8953 DietikonTel.: 044 745 13 13Fax: 044 745 13 10E-mail: [email protected]

UKVWR International LtdCustomer Service CentreHunter BoulevardMagna ParkLutterworthLeicestershireLE17 4XNTel.: 0800 22 33 44Fax: 01455 55 85 86E-mail: [email protected]

Your European Distribution Partner

A new range of screw neck vials withvolumes ranging from 4 – 60 ml are nowavailable to complement the VWRCollection wide range of chromatographyvials and accessories.

All vials are made of 1st class hydrolyticglass in clear or amber. As the screw neckvials require screw seals with differenttypes of thread, and as a broad selectionof various septa is available in the caps,closures should be asked for individually.The vials are in packs of 100.

For better identification of stored samplesbarcode labelling of the vials is alsopossible, however, a minimum orderquantity may be required.

Volume (ml) Type Thread Size (mm) Glass Cat. No.

4 Screw Neck 13-425 45 X 14,7 Clear 548-00514 Screw Neck 13-425 45 X 14,7 Amber 548-00528 Screw Neck 15-425 61 X 16,6 Clear 548-08218 Screw Neck 15-425 61 X 16,6 Amber 548-088912 Screw Neck 15-425 66 X 18,5 Clear 548-082012 Screw Neck 15-425 66 X 18,5 Amber 548-090320 Screw Neck 20-400 86 X 22,7 Clear 548-089020 EPA Screw Neck 57 X 27,5 Clear 548-015420 EPA Screw Neck 57 X 27,5 Amber 548-063830 EPA Screw Neck 72.5 X 27,5 Clear 548-015530 EPA Screw Neck 72.5 X 27,5 Amber 548-063740 EPA Screw Neck 95 X 27,5 Clear 548-015640 EPA Screw Neck 95 X 27,5 Amber 548-063960 EPA Screw Neck 140 X 27,5 Clear 548-064060 EPA Screw Neck 140 X 27,5 Amber 548-0641

New VWR Collection screw neck vials

For more information on the closures, septa or any other VWR Collection chromatography vialsplease contact your local VWR sales office.

Great value

Chrom Journal 3 EN:ChromMagazine EUR.qxdes.vwr-cmd.com/ex/downloads/magazine/chromjournal... · VWR ChromJournal Issue 3 September 2007 3 Flash chromatography is the fast and inexpensive

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