วารสารสัตวแพทยศาสตร์ มข. ปีที่ 22 ฉบับที่ 2 กรกฎาคม - ธันวาคม 2555 255 Identification of Jaagsiekte Sheep Retrovirus Infection in Sheep in Maedi- visna Virus Seropositive Farms in Ratchaburi Province, Thailand Trakarnsak Paethaisong 1 , Rattiya Naksuwan 1 , Philaiphon Chetiyawan 1 , Chongmas Antarasena 1* Abstract Case history—Between December 2010 and February 2012, necropsies were performed on five dead sheep submitted from two farms in Ratchaburi province, western Thailand. The animals were approxi- mately one to three years old. They showed signs of weight loss, chronic respiratory disease e.g. dys- pnea and respiratory distress, and finally fatal. Histopathology examination and virological tests were performed. In addition, serum samples of sheep from the same farms were tested for antibodies against Maedi-visna virus (MVV) by indirect ELISA. Clinical findings—Respiratory tracts of the affected sheep were filled with frothy fluid. Lungs were enlarged, heavy, edematous, and failed to collapse. Grayish-white multiple nodular lesions of 0.5-3 cm. in diameter were observed scatter through lung parenchyma. Microscopic picture revealed the formation of neoplastic foci composed of one layer of columnar epithelial cells arranged in acinar and papillary pat- tern. The neoplasms were surrounded by a large number of alveolar macrophages. Moreover, bronchiolar lumen was overflowing by proliferation of bronchiolar epithelium was also observed. Jaagsiekte sheep retrovirus (JSRV), the etiological agent of ovine pulmonary adenocarcinoma (OPA), was amplified from neoplastic lung tissues of five sheep by using real-time PCR. The seroprevalence of MVV infection in sheep aged over one year in the two farms were 75.38% (49/65) and 5.26% (1/19). These results provide evidence and confirm the presence of natural OPA coexisted with MVV in sheep in western Thailand. Conclusion—This is the first report describing the occurrence of OPA coexisting with MVV in sheep in western Thailand. Farmers should be advised to remove affected-OPA and MVV- infected sheep, includ- ing their offspring’s, to prevent the spread of these diseases. KKU Vet J. 2012;22(2):255-271. http://vmj.kku.ac.th/ Keywords: Ovine pulmonary adenocarcinoma; Maedi-visna virus; Jaagsiekte sheep retrovirus; Sheep; Ratchaburi province 1 Veterinary Research and Development Center (Western Region), Po. Box 18, Chom bueng District, Ratchaburi Province, 70150 * Corresponding author: Tel. 0-3222-8419, Fax. 0-3222-8379 ext. 114, E-mail: [email protected]CASE REPORT
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Case history—Between December 2010 and February 2012, necropsies were performed on five dead sheep submitted from two farms in Ratchaburi province, western Thailand. The animals were approxi-mately one to three years old. They showed signs of weight loss, chronic respiratory disease e.g. dys-pnea and respiratory distress, and finally fatal. Histopathology examination and virological tests were performed. In addition, serum samples of sheep from the same farms were tested for antibodies against Maedi-visna virus (MVV) by indirect ELISA.
Clinical findings—Respiratory tracts of the affected sheep were filled with frothy fluid. Lungs were enlarged, heavy, edematous, and failed to collapse. Grayish-white multiple nodular lesions of 0.5-3 cm. in diameter were observed scatter through lung parenchyma. Microscopic picture revealed the formation of neoplastic foci composed of one layer of columnar epithelial cells arranged in acinar and papillary pat-tern. The neoplasms were surrounded by a large number of alveolar macrophages. Moreover, bronchiolar lumen was overflowing by proliferation of bronchiolar epithelium was also observed. Jaagsiekte sheep retrovirus (JSRV), the etiological agent of ovine pulmonary adenocarcinoma (OPA), was amplified from neoplastic lung tissues of five sheep by using real-time PCR. The seroprevalence of MVV infection in sheep aged over one year in the two farms were 75.38% (49/65) and 5.26% (1/19). These results provide evidence and confirm the presence of natural OPA coexisted with MVV in sheep in western Thailand.
Conclusion—This is the first report describing the occurrence of OPA coexisting with MVV in sheep in western Thailand. Farmers should be advised to remove affected-OPA and MVV- infected sheep, includ-ing their offspring’s, to prevent the spread of these diseases.
KKU Vet J. 2012;22(2):255-271. http://vmj.kku.ac.th/
A typical gross lesion of a naturally JSRV-infected sheep was lung enlargement and failed to collapse, heavy and edematous. Grayish-white numerous tumors were found with various sizes from small nodules to solid mass, 0.5-3.0 cm in diameter (arrow) (panel A). Panel B revealed cut surface of affected lung showing dense and glistening appearance (arrow head).
Figure 2. Microscopic Findings of Lung Obtained from a JSRV Naturally JSRV-infected Sheep
Figure 2A, Neoplastic epithelial cells arranged in acinar (arrow head) and papillary pattern (arrow) (original magnification × 200). Figure 2B, The neoplastic cells which is columnar in shaped, forms papillary structures along the surface of thin fibro vascular cores (original magnification × 400). Figure 2C, Adenocarcinoma, acinar subtype. The large numbers of alveolar macrophages infiltrated around the neoplasms (arrow head) (original magnification × 400). Figure 2D, Proliferation of neoplastic bronchiolar secretary epithelial cells protrudes into the bronchiolar lumen (original magnification × 200). (H&E staining)
1. De las Heras M, Ortín A, Salvatori D, de Villareal MP, Cousens C, Ferrer LM, Cebrían LM, García de Jalón JA, González L, Sharp JM. A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions. Res Vet Sci. 2005;79: 259-264.
2. OIE (Office International des Epizooties). chapter 2.7.10. Ovine pulmonary adenocarcinoma (adenomatosis). Manual of Standards for diagnostic Tests and Vaccines for terrestrial animals (mammal, birds and bees). 6th edition, OIE, Paris, France; 2008. p. 1031-1035.
3. OIE (Office International des Epizooties). chapter 2.7.3/4. Caprine arthritis-encephalitis and maedi-visna. Manual of Standards for diagnostic Tests and Vaccines for terrestrial animals (mammal, birds and bees). 6th edition, OIE, Paris, France; 2008. p. 983-991.
4. CFSPH (Center for Food Security and Public Health). Maedi-Visna. 2007 [cited 2007 5 July 2007]; Available from: http://www.ivis.org/advances/Disease_Factsheets/maedi_visna. pdf.
5. CFSPH (Center for Food Security and Public Health). Ovine Pulmonary Adenomatosis. 2009 [cited 2009 18 August 2009]; Available from: http://www.cfsph.iastate.edu/ Factsheets/pdfs/ovine_pulmonary_adenomatosis.pdf.
6. Leroux C, Girard N, Cottin V, Greenland T, Mornex JF, Archer F. Jaagsiekte sheep retrovirus (JSRV): from virus to lung cancer in sheep. Vet Res. 2007; 38: 211-228.
7. Palmarini M, Cousens C, Dalziel RG, Bai J, Stedman K, DeMartini JC, Sharp, JM. The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep. J of Virol. 1996; 70 (3): 1618-1623.
8. Griffiths DJ, Martineau HM, Cousens C. Pathology and pathogenesis of ovine pulmonary adenocarcinoma. J Comp Pathol. 2010; 142 (4): 260-283.
9. Cousens C, Thonur L, Imlach S, Crawford J, Sales J, Griffiths DJ. Jaagsiekte sheep retrovirus is present at high concentration in lung fluid produced by ovine pulmonary adenocarcinoma-affected sheep and can survive for several weeks at ambient temperatures. Res Vet Sci. 2009; 87: 154-156.
10. Straub OC. Maedi-visna virus infection in sheep. History and present knowledge. Comp Immunol Microbiol Infect Dis. 2004; 27: 1–5.
11. Campbell JR, Menzies PI, Waltner-Toews D, Walton JS, Buckrell BC, Thorsen J. The seroprevalence of Maedi-visna in Ontario sheep flocks and its relationship to flock demographics and management practices. Can Vet J. 1994; 35:39–44.
12. Thormar H. Maedi-visna virus and its relationship to human immunodeficiency virus. AIDS Rev. 2005; 7(4): 233-245.
13. Herrmann LM, Hotzel I, Cheevers WP, On Top KP, Lewis GS, Knowles, DP. Seven new ovine progressive pneumonia virus (OPPV) field isolates from Dubois Idaho sheep comprise part of OPPV clade II based on surface envelope glycoprotein (SU) sequences. Virus Res. 2004; 102: 215–220.
14. De Andres D, Klein D, Watt NJ, Berriatua E, Torsteinsdottir S, Blacklaws BA, Harkiss GD. Diagnostic tests for small ruminant lentiviruses. Vet Microbiol. 2005 ; 107: 49-62.
15. Brinkhof J, Van Maanenn K. Detection and control of lentiviral infections in sheep and goats. Veterinary
Sciences Tomorrow. 2010 [cited 26 January 2010]. Available from : http://www.vetscite.org/publish/articles/ 000090/print.html
16. Pépin M, Vitu C, Russo P, Mornex JF, Peterhans, E. Maedi-visna virus infection in sheep: A review. Vet Res. 1998; 29: 341–367.
17. Rosadio RH, Sharp M, Lairmore D, Dahlberg JE, DeMartini JC. Lesions and retroviruses associated with naturally occurring ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Pathol. 1988; 25: 58-66.
18. Dawson M, Done SH, Venables C, Jenkins CE. Maedi-visna and sheep pulmonary adenomatosis : a study of concurrent infection. Br Vet J. 1990; 146(6): 531-538.
19. González L, Juste RA, Cuervo LA, Idigoras I, Saez de Ocariz C. Pathological and epidemiological aspects of the coexistence of maedi-visna and sheep pulmonary adenomatosis. Res Vet Sci. 1993; 54(2): 140-146.
20. Paethaisong T, Naksuwan, R, Chetiyawan P, Sakdinun P, Antarasena C. A preliminary report on the occurrence of Maedi-visna virus in sheep in Ratchaburi province. J of Animal Health Conference. 2011; 1: 37-48.
21. Palmarini M, Holland MJ, Cousens C, Dalziel RG, Sharp JM. Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis. J of Gen Virol. 1996; 77: 2991-2998.
22. Herrmann-Hoesing LM, Broughton-Neiswanger LE, Gouine KC, White SN, Mousel MR, Lewis GS, Marshall KL, Knowles DP. Evaluation of a caprine arthritis-encephalitis virus/Maedi-Visna virus indirect enzyme-linked immunosorbent assay in the serological diagnosis of ovine progressive pneumonia virus in U.S. sheep. Clin Vac Immunol. 2010; 17(2): 307-310.
23. IDEXX. IDEXX CAEV/MVV Total Ab Screening Test. Product information sheet. 2010 [cited 27 August 2012]; Available from : http://www.idexx.com/pubwebresources/pdf/en_us/livestock-poultry/caev-mvv-screening-test-sheet.pdf
24. Palmarini M, Fan H. Retrovirus-induced ovine pulmonary adenocarcinoma, an animal model for lung cancer. J Natl Cancer Inst. 2001; 93(21): 1603-1614.
25. Khodakaram-Tafti A, Razavi Z. Morphopathological study of naturally occurring ovine pulmonary adenocarcinoma in sheep in Fars province, Iran. Iran J Vet Res. 2010; 11(2): 134-138.
26. Oda SS, Youssef SA. Immunohistochemical and histopathological findings of ovine pulmonary adenocarcinoma (Jaagsiekte) in Egyptian sheep. Trop Anim Health Prod. 2011; 43: 1611-1615.
27. Kycko A, Reichert M. PCR-based methods for detection of JSRV in experimentally and naturally infected sheep. Bull Vet Inst Pulawy. 2010; 54: 445-450.
28. Ortín A, Minguijón E, Dewar P, García M, Palmarini M, González L, Sharp JM, De las Heras M. Lack of specific immunoresponse against recombinant capsid antigen of the sheep pulmonary adenomatosis retrovirus in sheep and goats naturally affected by enzootic intranasal tumour or jaagsiekte. Vet Immunol Immunopathol. 1998; 61: 229-237.
29. Fournier D, Campbell JR, Middleton DM. Prevalence of maedi-visna infection in culled ewes in Alberta. Can Vet J. 2006; 47: 460-466.
30. Zanoni RG, Nauta I M, Kuhnert P, Pauli U, Pohl B, Peterhans E. Genomic heterogeneity of small ruminant
lentiviruses detected by PCR. Vet Microbiol. 1992; 33: 341–351.
31. Information Technology Center, Department of Livestock Development.Statistics and number of sheep and sheep farmer in 2011.[cited 27 August 2012]; Available from: http://www.dld.go.th/ict/th/images/stories/stat_web/yearly/2554/sheep54/report_ sheep_54.pdf.
32. Sharp JM, DeMartini JC. Natural history of JSRV in sheep. Curr Top Microbiol Immunol. 2003; 275:55-79.