By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses.

The Treatment and Prevention of Dental

CariesBy: Scott Karas

Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses

Overview

What are they?◦ Cavities

How are they formed?◦ Streptococci mutans

How to prevent them?◦ Brush your teeth◦ Vaccine?

Dental Caries

GTF- Glucosiltransferase GbpB- Glucan binding protein B CAT- Catalytic Domain

◦ GTF epitope GLU- Glucan binding domain SYI- peptide complex

◦ GbpB epitope WIS-Water insoluble glucan

Key Enzymes/Antibodies

Purpose of this experiment Bacteria used

◦ Streptococci mutans◦ Streptococci sobrinus

Synthetic peptides Three experiments

◦ 24-37 day old rats Measured the levels of antibodies Measured dental caries

Experiment Overview

Peptides◦ Three monoepitopic◦ Two diepitopic

Proteins◦ GbpB from S. mutans◦ GTP from S. sobrinus

ELISA◦ Measured IgA and IgG antibody levels

Methods

Experiment 1◦ 40 female rats◦ 6 groups of 7◦ Injected subconsciously

Experiment 2- S. mutans◦ 48 female rats◦ 4 groups of 12 in a cage◦ Injected subconsciously◦ Asphyxiated

Cleaned with beetles and ethanol

Methods

Experiment 3- S. sobrinus◦ Identical to Experiment 2◦ Streptomycin-resistant S. sobrinus

Bacterial Recovery◦ ELISA◦ Cotton swab

Carie assessment

Methods

Human Trials◦ Put in cows milk or eggs

Treatment for children or infants◦ “Window of Infectivity”

Treat young mothers◦ Breast feeding

Potential Uses

Dental Caries Methods Results Potential Uses

Summary

Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses

Summary

Smith, Daniel J., William F. King, Joy Rivero, and Martin A. Taubman. "Immunological and Protective Effects of Diepitopic Subunit Dental Caries Vaccines." Infection and Immunity 73.5 (2005): 2797-804. Print.

Russell, Michael W., Noel K. Childers, Suzanne M. Michalek, Daniel J. Smith, and Martin A. Taubman. "A Caries Vaccine?" ProQuest Nursing & Allied Health Source 38.3 (2004): 230-35. Print.

References