DAFTAR PUSTAKA 1. Houck MM, Siegel JA. Fundamentals of Forensic Science [Internet]. Elsevier Science; 2015 [cited 2016 Jan 9]. 736 p. Available from: https://books.google.com/books?id=8dicBAAAQBAJ&pgis=1 2. Houck M. Forensic Classification of Evidence. In: Forensic Biology [Internet]. 2015. p. 298. Available from: http://books.google.com.br/books?id=JJgpAQAAMAAJ 3. Syukriani Y. DNA Forensik. Jakarta: Sagung Seto; 2012. 4. Butler JM. Fundamentals of Forensic DNA Typing. Fundam Forensic DNA Typing [Internet]. 2010;363–96. Available from: http://www.sciencedirect.com/science/article/pii/B9780123749994000163 5. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. From DNA to RNA [Internet]. Garland Science; 2002 [cited 2016 Jan 15]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK26887/ 6. National Institute of Health US. Cells and DNA. 2015; 7. Deedrick DW. FBI — Hairs, Fibers, Crime, and Evidence, Part 1 [Internet]. Forensic science communication. 2000 [cited 2016 Jan 16]. Available from: https://www.fbi.gov/about-us/lab/forensic-science- communications/fsc/july2000/deedric1.htm/ 8. Nilsson M, Budowle B, Andre H, Lundberg H, Allen M. Nuclear and mitochondrial DNA quantification of various forensic materials. 2006;164:56–64. 9. Schneider MR, Schmidt-ullrich R. The Hair Follicle as a Dynamic Miniorgan. Curr Biol [Internet]. Elsevier Ltd; 2009;19(3):R132–42. Available from: http://dx.doi.org/10.1016/j.cub.2008.12.005 10. Friis C, Olsen ME, Ørsted L, Bertelsen MF, Willerslev E, Tobin DJ, et al. DNA from keratinous tissue . Part I : Hair and nail. Ann Anat [Internet]. Elsevier GmbH.; 2012;194(1):17–25. Available from: http://dx.doi.org/10.1016/j.aanat.2011.03.013 11. (SWGMAT) SWG on MA. Standards and Guidelines - Forensic Human Hair Examination Guidleines [Internet]. Forensic science communication. 2005 [cited 2016 Jan 16]. Available from: https://www.fbi.gov/about- us/lab/forensic-science- communications/fsc/april2005/index.htm/standards/2005_04_standards02.h tm 12. Ghatak S, Muthukumaran RB, Nachimuthu SK. A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis. J Biomol Tech. 2013;224–31. 13. Pray L. Discovery of DNA Double Helix: Watson and Crick [Internet]. Nature Education. 2008 [cited 2016 Jan 21]. Available from: http://www.nature.com/scitable/topicpage/discovery-of-dna-structure-and- function-watson-397 14. Calladine C. Understanding DNA. Igarss 2014. 2014. 1-5 p.
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61
DAFTAR PUSTAKA
1. Houck MM, Siegel JA. Fundamentals of Forensic Science [Internet].
Elsevier Science; 2015 [cited 2016 Jan 9]. 736 p. Available from: https://books.google.com/books?id=8dicBAAAQBAJ&pgis=1
2. Houck M. Forensic Classification of Evidence. In: Forensic Biology [Internet]. 2015. p. 298. Available from: http://books.google.com.br/books?id=JJgpAQAAMAAJ
3. Syukriani Y. DNA Forensik. Jakarta: Sagung Seto; 2012. 4. Butler JM. Fundamentals of Forensic DNA Typing. Fundam Forensic DNA
Typing [Internet]. 2010;363–96. Available from: http://www.sciencedirect.com/science/article/pii/B9780123749994000163
5. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. From DNA to RNA [Internet]. Garland Science; 2002 [cited 2016 Jan 15]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK26887/
6. National Institute of Health US. Cells and DNA. 2015; 7. Deedrick DW. FBI — Hairs, Fibers, Crime, and Evidence, Part 1 [Internet].
Forensic science communication. 2000 [cited 2016 Jan 16]. Available from: https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2000/deedric1.htm/
8. Nilsson M, Budowle B, Andre H, Lundberg H, Allen M. Nuclear and mitochondrial DNA quantification of various forensic materials. 2006;164:56–64.
9. Schneider MR, Schmidt-ullrich R. The Hair Follicle as a Dynamic Miniorgan. Curr Biol [Internet]. Elsevier Ltd; 2009;19(3):R132–42. Available from: http://dx.doi.org/10.1016/j.cub.2008.12.005
10. Friis C, Olsen ME, Ørsted L, Bertelsen MF, Willerslev E, Tobin DJ, et al. DNA from keratinous tissue . Part I : Hair and nail. Ann Anat [Internet]. Elsevier GmbH.; 2012;194(1):17–25. Available from: http://dx.doi.org/10.1016/j.aanat.2011.03.013
11. (SWGMAT) SWG on MA. Standards and Guidelines - Forensic Human Hair Examination Guidleines [Internet]. Forensic science communication. 2005 [cited 2016 Jan 16]. Available from: https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/april2005/index.htm/standards/2005_04_standards02.htm
12. Ghatak S, Muthukumaran RB, Nachimuthu SK. A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis. J Biomol Tech. 2013;224–31.
13. Pray L. Discovery of DNA Double Helix: Watson and Crick [Internet]. Nature Education. 2008 [cited 2016 Jan 21]. Available from: http://www.nature.com/scitable/topicpage/discovery-of-dna-structure-and-function-watson-397
14. Calladine C. Understanding DNA. Igarss 2014. 2014. 1-5 p.
62
15. Stubbs M, Suleyman N. Crash Course: Cell Biology and Genetics [Internet]. Elsevier Health Sciences; 2013 [cited 2016 Jan 21]. 240 p. Available from: https://books.google.com/books?id=UbTRAQAAQBAJ&pgis=1
16. The Nucleus [Internet]. 2010 [cited 2016 Jan 22]. Available from: http://www.apsubiology.org/anatomy/2010/2010_Exam_Reviews/Exam_1_Review/Ch03_The_Nucleus.htm
17. Bertino A. Forensic Science: Fundamentals and Investigations 2012 Update [Internet]. Cengage Learning; 2011 [cited 2016 Jan 9]. 608 p. Available from: https://books.google.com/books?id=qUQJAAAAQBAJ&pgis=1
18. NFSTC Science Serving Justice. DNA Analyst Training [Internet]. 2007 [cited 2016 Jan 22]. Available from: http://projects.nfstc.org/pdi/Subject02/pdi_s02_m02_01_a.htm
19. Mcnevin D, Wilson-wilde L, Robertson J, Kyd J, Lennard C. Short tandem repeat ( STR ) genotyping of keratinised hair Part 1 . Review of current status and knowledge gaps. 2005;153:237–46.
20. Initiative D. DNA Extraction & Quantitation for Forensic Analysts [Internet]. 2008. Available from: www.DNA.gov
21. Li R. Forensic Biology, Second Edition [Internet]. CRC Press; 2015 [cited 2016 Jan 23]. 567 p. Available from: https://books.google.com/books?id=ZHcZBwAAQBAJ&pgis=1
22. Shimizu H. Shimizu Dermatology [Internet]. Wiley; 2016 [cited 2016 Jan 16]. 464 p. Available from: https://books.google.com/books?id=vXFJjgEACAAJ&pgis=1
23. Saferstein R, Meloan C, James R. Lab Manual for Criminalistics: An Introduction to Forensic Science [Internet]. Pearson College Division; 2014 [cited 2016 Jan 16]. 320 p. Available from: https://books.google.com/books?id=19WQngEACAAJ&pgis=1
24. Buffoli B, Rinaldi F, Labanca M, Sorbellini E, Trink A, Guanziroli2 E, et al. The human hair: from anatomy to physiology. Int J Dermatol [Internet]. 2014 [cited 2016 Jan 9];331–41. Available from: http://moscow.sci-hub.bz/da4e6f76349ede9dcc6ba53561db52cb/10.1111%40ijd.12362.pdf
25. FBI. Basic Structure of Hair. Univ South Carolina [Internet]. 2005;11. Available from: http://www.chem.sc.edu/analytical/chem107/lab4_032205.pdf\nhttp://www.fbi.gov
26. Harkey MR. Anatomy and physiology of hair. Forensic Sci Int. 1993;63(1-3):9–18.
27. NFSTC Science Serving Justice. Hair [Internet]. 2007 [cited 2016 Jan 23]. Available from: http://projects.nfstc.org/pdi/Subject02/pdi_s02_m02_10.htm
28. Serrano-Falcón C, Fernández-Pugnaire M a, Serrano-Ortega S. Hair and scalp evaluation: the trichogram. Actas Dermosifiliogr [Internet]. AEDV; 2013;104(10):867–76. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23871642
29. Sperling LC. Hair anatomy for the clinician. J Am Acad Dermatol [Internet]. American Academy of Dermatology, Inc; 1991;25(1 Pt 1):1–17. Available
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from: http://dx.doi.org/10.1016/0190-9622(91)70167-Z 30. Andréasson H, Nilsson M, Budowle B, Lundberg H, Allen M. Nuclear and
mitochondrial DNA quantification of various forensic materials. Forensic Sci Int [Internet]. 2006;164(1):56–64. Available from: http://www.sciencedirect.com/science/article/pii/S0379073805006250
31. Harland DP, Vernon JA, Walls RJ, Woods JL. Transmission electron microscopy staining methods for the cortex of human hair: A modified osmium method and comparison with other stains. J Microsc. 2011;243(2):184–96.
32. Morioka K. A guide to hair follicle analysis by transmission electron microscopy: Technique and practice. Exp Dermatol. 2009;18(7):577–82.
33. Hong EP, Park JW. Sample size and statistical power calculation in genetic association studies. Genomics Inform [Internet]. 2012;10(2):117–22. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3480678&tool=pmcentrez&rendertype=abstract
34. Identification C. Assessment of Nucleic Acid Purity. Thermo Fish Sci [Internet]. 2012; Available from: http://www.nanodrop.com/Library/T042-NanoDrop-Spectrophotometers-Nucleic-Acid-Purity-Ratios.pdf
35. Thermo Scientific. 260/280 and 260/230 Ratios. 2008;1–2. Available from: http://www.nanodrop.com/Library/T009-NanoDrop 1000-&-NanoDrop 8000-Nucleic-Acid-Purity-Ratios.pdf
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Lampiran 1. Ethical Clearance
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Lampiran 2. Surat Izin Penelitianx
66
Lampiran 3. Informed Consent
JUDUL PENELITIAN : Perbedaan Kuantitas DNA yang Diekstrak dari Akar Rambut
Berbagai Fase Pertumbuhan
INSTANSI PELAKSANA : SMF Forensik Fakultas Kedokteran Universitas Diponegoro, Unit
Pelayanan Terpadu (UPT) Laboratorium Bakteriologi Universitas Diponegoro
PERSETUJUAN SETELAH PENJELASAN
(INFORMED CONSENT)
Yth:……
Perkenalkan nama saya Valensa Yosephi. Saya mahasiswa Program Studi Pendidikan
Dokter Fakultas Kedokteran UNDIP. Untuk mendapatkan gelar Sarjana Kedokteran, salah satu
syarat yang ditetapkan kepada saya adalah menyusun sebuah penelitian. Penelitian yang saya
lakukan berjudul “Perbedaan Kuantitas DNA yang Diekstrak dari Akar Rambut Berbagai Fase
Pertumbuhan.”
Tujuan dari penelitian ini adalah menganalisis perbedaan kuantitas DNA yang
diekstrak dari akar rambut berbagai fase pertumbuhan. Dalam penelitian ini saya akan
memerlukan sampel rambut kepala manusia. Untuk itu saya minta kesediaan Saudara untuk
menjadi relawan pada penelitian ini. Pengambilan sampel rambut dilakukan dengan penyisiran
rambut untuk mendapatkan helaian rambut rontok dan pencabutan rambut untuk mendapatkan
total 70 helai rambut yang akan diperiksa di bawah mikroskop. Saudara yang bersedia diminta
untuk mencuci rambut dengan air mengalir tanpa sabun pembersih rambut atau sampo sebelum
pengambilan sampel rambut. Pencabutan rambut dilakukan dengan teknik steril menggunakan
pinset, sarung tangan, masker steril. Pencabutan dilakukan dengan hati-hati dan dengan teknik
yang tepat untuk meminimalkan risiko tidak nyaman saat pencabutan.
Manfaat dari penelitian ini adalah dapat memberikan informasi mengenai perbedaan
kuantitas DNA yang diekstrak dari akar rambut berbagai fase pertumbuhan dan memberikan
data mengenai kisaran kuantitas DNA yang dapat diekstrak dari akar rambut dalam fase anagen,
katagen, dan telogen. Bagi laboratorium forensik, hasil penelitian dapat digunakan sebagai
pertimbangan apabila akan dilakukan pemeriksaan identifikasi DNA dari sampel rambut dan
sebagai landasan bagi penelitian selanjutnya untuk lebih mendalami dan menyempurnakan
pemahaman mengenai identifikasi DNA dari sampel rambut. Saya mohon kesediaan Anda
untuk ikut serta dalam penelitian ini secara sukarela.