Application of 3D printing in analytical chemistry (by Fariborz Amoozgar)

by :Fariborz Amoozgar Email:[email protected]

APPLICATION OF 3D PRINTING IN ANALYTICAL CHEMISTRY AND SEPRATION

Supervisor:

Presented by:

Fariborz Amoozgar


Application

How 3D printer works?

History

Contents

Introduction

Conclusion

Methods & Technologies


3D printing or additive manufacturing is a process of making three dimensional solid

objects from a digital file. The creation of a 3D printed object is achieved using additive

processes.

INTROUCTION :


Has been around since the early 80’s modern 3D Printing by:

Charles W .Hull , stereolithography technique.


by :Fariborz Amoozgar Email:fariborz


Methods and technologies of 3D Printing:


SLA (Stereolithography):

the laser beam traces a cross-section of the part pattern on the surface of the

liquid resin. Exposure to the ultraviolet laser light cures and solidifies the pattern

traced on the resin and joins it to the layer below.


SLS(Selective laser sintering):

The laser selectively fuses the powdered material by scanning the cross-sections

(or layers) generated by the 3D modeling program on the surface of a powder bed.


FDM (Fused deposition modeling):

The FDM technology works using a plastic filament or metal wire which is unwound

from a coil and supplies material to an extrusion nozzle .The nozzle is heated to melt

the material by a numerically controlled mechanism. The object is produced by

extruding melted material to form layers as the material hardens immediately after

extrusion from the nozzle.


LOM(Laminated object manufacturing):

after the first layer of a sheet material is loaded onto a stage, a laser or razor traces the

designed cross-section to define the pattern on the layer. After the excess material of the

sheet is removed, a second layer covers the previous layer and the laser or knife tracing will

define the next pattern based on information in the .STL file. Adjacent layers are combined

by use of adhesives or welding for paper or metal, respectively. These steps are repeated to

generate a layered 3D model.


Production companies:

1)Objet Geometrise

2) Z-Corporation

3) Stratasys


RepRap Version 1.0 RepRap Version 2.0

Household 3D printers :


http://en.wikipedia.org/wiki/File:Reprap_Darwin.jpg

http://en.wikipedia.org/wiki/File:Reprap_Darwin.jpg

http://en.wikipedia.org/wiki/File:RepRap_'Mendel'.jpg

http://en.wikipedia.org/wiki/File:RepRap_'Mendel'.jpg

The cost of 3D Printers:


3D Printing

Food

Industries

APPLICATIONS Medical

ARCHITECTURE

Weaponry &

Archaeology and

paleontology

Household uses

Mathematics

&Robotics


Physicians can use 3D printing to

make hearing aids, artificial teeth, and bone

grafts.

• 3D printed Jaw

• 3D printed Ear

• 3D Printed bone

MEDICAL INDUSTRY


Artists can create models of their projects.

ARCHITECTURE


Designers use 3D printers to quickly create product models

and prototypes.

INDUSTRIAL DESIGN


Archaeology and paleontology

Weaponry


Robotics

Mathematics


Household uses

‘Home Printed’ Food


FIRST EVER 3D PRINTED CAR


Building

24

This printer is built by Pro . Behrokh Khoshnevis.It can build a 200 square meter house in only 24 hours.


25

Chemistry

Printing Lab On Chip (LOC) devices and interconnect

Printing Biosensors and electrodes for electrochemical reaction

Printing chromatography columns

Printing flow – cells

Printing mili fluidic devices and micro fluidic devices


The interconnect, a flexible polymer gasket co-printed with, rigid clamps, elim-inatesadhesives and additional assembly by direct multi-material 3D, printing from a computer-aideddesign model.


The maximum pressure that the system can not tolerate:

Ffluid >Ffriction Leakage


Schematic of the fluidic circuit for the

durability tests and modified dead-end

channel experiments .

Experimental setup used to conduct long-term reliability testing.



Interconnects delivering three different fluids, via adhesive-free connections, into separate

channels have also been demonstrated.

3D printed porous media columns with fine control of column packing morphology:

The three bed geometric designs: 1- simple cubic beads, 2- straight channels, 3- herringbone channels


Illustration of the flow distributor templates:

(a) radial flow distributor, (b) fractal flow distributor

where Ѳ is the dimensionless time defined in terms of the theoret-ical residence time, estimated from the designed geometry of the lattice structure.


The SC coloumn is better than the other due to have a Ѳ close to one.


Comparison of 2 ml columns with radial and fractal flow distributors for (a) PC columns

(b) SC columns.


3D-printedand CNC milled flow-cells for chemiluminescence detection:

CNC milling:A model of each flow-cell was drawn using the Auto

Desk Inventor And the models were converted Intomachine code using Edge CAM software.


(i) flow-cell A (ii) flow-cell A with a mirror against the back face (iii) flow-cell A in the purpose-built holder(iv) flow cell F in the same holder by :Fariborz Amoozgar Email:[email protected]

A: coiled tubing

B: 3D-printed

transparent polycarbonate milled

F: white Acetal milled

reaction of morphine with the permanganate reagent:


a) Dual detection zone flow-cell

b) conventional T-piece splitting to two separate flow-cells

O: octopamine S: synephrineT: tyramineH: hordenine


Low cost lab-on-a- chip prototyping with a consumer grade 3D printer:

In this work, affordable 3D printed LOC devices have been demonstrated. Complex geometries,

directly created in 3D printed structures, enable transference of demanding fabrication tasks to the

printer, thus maximizing reliability and removing the influence of user fabrication skills from the

prototypes.


For H2O2 detection with ULOC, connectors were assembled with silicon tubing:C) control solution S) 0.1μM (sample)H)1μM (high level)


Preparation:

1- Pumping MPs and anchored by external magnet2- Washing Extra3- modified with biotinyled Glycan4- Washing Extra5- Binding of HA-CdS on to biotinyled Glycan 6-Washing Extra7-immerseing the chip in an ultra sonic bath to fractionaing to MPs-Glycan-HA-Cds complex

3D printed chip for electrochemical detection of influenza virus labeled with CdS

quantum dots:


a) Injection (influx) was used for dispensing the

samples , buffer and electrolyte

a) Reaction cell,where whole process of isolation and

magnetic pad,(d)was placed.

c) Three electrod setup,with working glassy carbon

microelectrode,andefflux

e) For removing of reactants from the reaction


Effect of isolation and detection procedure on the Real sample detection.Real sample is inactivated influenzavirusH5N1

S1-S4 are the same samples and k1 and k2 are the negative controls


Configurable 3D-Printed millifluidic and microfluidic ‘lab on a chip’ reactionware devices:

An overview of the time and cost associated with the

fabrication of each of the three reactors, along with

their overall dimensions

R1; A two inlet device, (top right), R2; a three-inlet

device, (below) R3; a one-inlet device with two

‘‘silos’’: one filled with sodium molybdate


R2: The actual set-up of the devices, with three inlets each

connected to a pump, and the in-line ATR-IR and/or UV-Vis flow-cells connected to the outlet.


R1 : Flow synthesis of the imine derived from benzaldehyde and

benzylamine, as characterised by in-line ATR-IR spectroscopy.


A 3D Printed Fluidic Device that Enables Integrated Features:


Rapid prototyping

Clean process

Complex shape

Easy to use

Reduce design complexity

Cheap in massive production

Remote location fabrication

ADVANTAGES


Process is slow

Components do not have enough strength.

Cost of raw materials

3-D printers are still expensive.

Misuse of technology

Although 3-D printers have the potential of creating many jobs and opportunities, they might also put certain jobs at risk .

DISADVANTAGES


3D printing is rapidly maturing

Still a lot to discover

Can save lives (literally)

May disrupt property and manufacturing processes

Ethical and law questions need to be solved

Potentially very dangerous

Conclusion


Bethany C. Gross, Jayda L. Erkal, Sarah Y. Lockwood, Chengpeng Chen, and Dana M

Spence .Anal. Chem., Just Accepted Manuscript

ringsO.H. Paydara,∗, C.N. Paredesb, Y. Hwangb, J. Pazb,c, N.B. Shahb, R.N. Candlerb,

3D printedchipforelectrochemicaldetectionofinfluenza viruslabeled with CdSquantumdots

Chen Zhao, CaiyunWang, Robert Gorkin III, Stephen Beirne, Kewei Shu, Gordon

G.Wallace Kara B.Spilstead a, JessicaJ.Learey a, EganH.Doeven a,nn, GregoryJ.Barbante a, StephanMohr b,

NeilW.Barnett a, JessicaM.Terry a, RobynneM.Hall c, PaulS.Francis Kari B. Anderson,† Sarah Y. Lockwood,† R. Scott Martin,§ and Dana M. Spence R. S.; Takayama, S.; Otsuni, E.; Ingber, D. E.; Whitesides, G. M. Biomaterials 1999, 20, 2363- 2376.Waldbaur, A.; Rapp, H.; Lange, K.; Rapp, B. E. Analytical Methods 2011, 3, 2681-2716. Germán Comina,a Anke Suskaa and Daniel Filippini Philip J. Kitson, Mali H. Rosnes, Victor Sans, Vincenza Dragone and Leroy Cronin*

REFERENCES



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