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Additional files
Figure S1 - Characteristic analysis of the homology search for
transcriptome unigenes
against the NR database
(A)E-value distribution of BLAST hits for each unique sequence
with a cut-off E-value of
1.0E-5; (B) Similarity in distribution of the top BLAST hits for
each sequence; (C) Species
distribution is shown as a percentage of the total homologous
sequences with an E-value of at
least 1.0E-5. We used the first hit of each sequence for
analysis.
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Figure S2 - Histogram presentation of the (A) gene ontology (GO)
and (B) clusters of
orthologous group (COG) classification
(A) The results are listed in three categories: biological
process, cellular component and
molecular function. (B) Out of 28,779 BLAST hits, 10,784
sequences are classified into 25
COG categories.
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Figure S3 – Different components of the raw tags and
distribution of distinct tags in
each sample.
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Figure S4 –Distribution of total tags and distinct tags in each
sample.
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Figure S5 – The gene expression level in all comparisons.
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Table S1 – Sample collection and RNA extraction strategy.
(XLSX)
Table S2 – The specific primers used in qRT-PCR to validate
differentially expressed
genes.
(XLSX)
Table S3 – Use of Sanger sequencing to verify the quality of
RNA-seq results.
(XLSX)
Table S4 – Top hits obtained by BLASTX against NR database for
the total unigenes.
(XLSX)
Table S5 – The GO annotation of unigenes.
(XLSX)
Table S6 – KO annotation of unigenes.
(XLSX)
Table S7 – Tag analysis statistics.
(XLSX)
Table S8 –Differentially expressed tags were filtered with the
absolute value of
log2Ratio ≥ 1 based on the FDR < 0.001 from the tandem
comparison of samples i.e.,
LD vs DT, PD vs DT, ED vs LD, ED vs PD, ED vs DT, LD vs PD.
(XLSX)
Table S9 – GO enrichment analysis in different comparisons.
(DOC)
Table S10 – KO enrichment analysis in different comparisons.
(XLSX)
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Table S11– Differentially expressed genes filtered
accordingtothe absolute value of
log2Ratio ≥ 1 based on the FDR < 0.001 among ED, LD and PD
were annotated
indifferent databases.
(XLSX)
Table S12– Putatively identified Heat shock protein were
filtered with the absolute
value of log2Ratio ≥ 1 based on the FDR < 0.001 among ED, LD
and PD.
(XLSX)