(980 nm) UC(550 nm) Trypan Blue 6 h A;49 %) YPO 4sogalabo.jp/ptrcar/H19PTRC_2.pdf · 2010-06-12 · Deregulated expression of a novel component of TFTC/STAGA histone acetyltransferase

(980 nm ) U C (550 nm ) Trypan B lue 6 h

100 % 24 h 70 %72 h 3 Y 38 %

45 % ( C ;42 % , G ;44 % , A ;49 % ) Y PO 4

75 %

H 5 Y 2O 3: Er U C6 100

24 70 72 40 50 Y PO 4

75

1) Fang Lim et al, "In V ivo and Scanning Electron M icroscopy Im aging of U pconverting N anophosphors in C aenorhabditis elegans," Nano Letters, 6 [2] (2006)169-174. 2) D avid Schubert et al, "C erium and yttrium oxide nanoparticles are neuroprotective," Biochemical and Biophysical Research Communications, 342 (2006) 86-91.

100

80

60

40

20

0

Sam plesY C G A P

Y 2O 3: Er

Y 2O 3: Er(a) (c) (980 nm ,550 nm)

(FB I)

FB I

FB I

in vivo FB I

nm 500nm

980 nm 1550 nm

Y PO 4: Er in vivo

Y PO 4: Er

m ol 10

K 3PO 4 1100 1

X (FT-IR ) pH 3 H C l 2

pH

5 m ol/l

X

FT-IR (SEM ) 980 nm

Y PO 4: Er O P-50

Y PO 4: Er

U C 980

nm 565 nm Y 2O 3:Er

FT-IR Y PO 4

Y PO 4

K 3Y (PO 4)22 Y PO 4 Y PO 4

K 3Y (PO 4)2 Y PO 4

K 3Y (PO 4)2 K 3(Y PO 4)2Y PO 4: Er FT-IR X

Y PO 4 Y PO 4

Y PO 4 10 Y PO 4

480

Y PO 4 Y PO 4

SEM 500 nm Y PO 4:Er 980 nm

1550 nm 125m W

1s 3(a),(b)

Y 2O 3: Er 900m W 1s 980 nm

U C ( 4(c),(d)

K 3PO 4 500

nm Y PO 4: Er Y PO 4: Er

Y 2O 3: Er

400 500 600 700 800 9000

0.5

1

w avenum ber(cm-1)

Y PO 4Y PO 4

10 20 30 40 50 600

1000

2000

2 (degree)

Y PO 4Y PO 4K 3Y (PO 4)2

3

(a) (b) 4 U C

(a) (b)

T

A ILIM /IC O S T

elongation (1) elongation

T elongation

A ILIM /IC O S T elongation

A ILIM /IC O S T A ILIM /IC O S

elongation C EM T PB T

T elongation

T m R N A

K IF3A K IFC 3 K IF5B K IF9 K LC (2)

dom inant negative A ILIM /IC O S C EM

A ILIM /IC O S K IF3A elongation

K IF3A dom inant negative PB T (Fig.

1)

K IF3A PA R 3 aPK C

(3) G FP-aPK C

C EM elongation

G FP-aPK C T elongation PA R 3 aPK C

dom inant negative PA R 3 aPK C

elongation (Fig. 2)

T elongation K IF3A PA R 3 aPK C

T K IF3A PA R 3 aPK C T

H U V EC

(1) O kam oto N ., N ukada Y., Tezuka K ., et al., Int Immunol 16, 1515-22 (2) R iley L. J, M ao M ., K obayashi S., et al., Proc. Natl Acad. Sci. USA 99, 11790-11795 (3) N ishim ura T., K ato K ., Yam aguchi T., et al., et al, Nat Cell Biol 6, 328-34

Fig.1 K IF3A dom inant negative T elongation

C EM PB T K IF3A w ild type (w t) dom inant negative (D N ) m ock

elo n g atio n

a

b.

c. d.

Fig.2 PA R , aPK C dom inant negative T elongation

c.a. b. d.s.

C EM PB T PA R 3 aPK C w ild type (w t) dom inant negative (D N )

m ock elongation

A B C

K 2

K 2

side population (SP) A B C

H oechst

SP 1 106 K 2 A B C

50 M verapam il 37 30

20 g/m l H oechst 37 60 1 g/m l propidium iodide (PI) 37

10 PI

H oechst SP

K 2 activin A FG F2 H G F

K 2

poly-H em dish 15 FC S-D M EM 2

dish 100 ng/m l activin A FG F2 1 % FC S-D M EM /F12

3 10 ng/m l H G F 1% D M SO 10 %

FC S-D M EM /F12 5 10-7 M

2 K 2 R N A

4K 2 SP K 2

61% SP (Fig.1)

H oechst A B C verapam il SP

0.3% SP

Fig. 1 Existence of SP cells in rat hepatom a K 2 cells.

K 2 K 2 2

activin A FG F2 3

H G F D M SO 5

7 20% , 3%

2 TATsox17 pdx1

1 K 2

K 2

K 2

ribosom e inactivating protein (R IP)

(Aralia elata) aralin

N- A B(1) A ralin R IP

ricin aralin R IP

aralin

aralin

aralin

A ralin C y3 0.1 M N aH C O 3 4 30

G 50 C y3-aralin B SA Lys C y3

C y3-B SA C y3-Lys A ralin

W I-38 VA -13

H eLa H oechst33342 C y3-aralin C y3-B SA 4

15 aralin

H eLa C y3-aralin 10 aralin

aralin Far W estern blot H eLa

SD S-PA G E

aralin aralin H R P

X aralin

aralin H eLa

aralin Far W estern blot 4

A ralin C y3-aralin H eLa VA -13

W I-38 C y3-aralin aralin

(Fig1) C y3-aralin

aralin aralin C y3-aralin

C y3-aralin aralin

H eLa Far W estern blot 100

kD a A ralin

100 kD a aralin

Cy3 Hoechst Phase Merge

Fig1 C y3-aralin

A ralin 100 kD a

A ralin

LF(Low Frequency)

1 iC C D 5ns

LF H e

2

LF

O 2 H

(H O O )

(Electron Spin R esonance)

1 LF 2

2008 3 13

2. Y 2O 3:Er SEM ((a) PEG ,

(b)PEG (PEG 0.019 chains/nm 2))

3 . PEG Y 2O 3:Er (PEG

0.019 chains/nm 2) U C

(980 nm )

1) 2nd W orkshop on P hotolum inescence in R are E arths: P hotonic M aterials and D evices

(P R E’07) (Tokyo U niv. of Sci.)Yu Saito, K . Shim izu, M . Shim izu, T. K onishi and K . Soga

“A pplication of rare-earth-doped phoshpors for N IR bioim aging” (2007/5/31-6/1, Trento, Italy)

2) M aterials Science and Technology 2007 C onference and E xhibition (M S& T ’07) (Tokyo U niv.

of Sci.) K ohei Soga, Yu Saito, K azuaki Shim izu, Yoshihito O kum ura and Tom oya K onishi,

“A pplication of R are-E arth D oped C eram ic N anophoshpors for B ioim aging under N IR

E xcitation” (2007/9/16-20, D etroit, M ichigan, U SA ).

3) Second International Sym posium on A tom ic Technology (Tokyo U niversity of Science)

K ohei Soga, "D evelopm ent of N IR B ioim aging System s by “Polyscale” T hechnologies Starting

at A tom ic Technologies," (2007/10/1-2, Aw aji, H yogo).

4) Second International Symposium on Atomic Technology (Tokyo Univ. of Sci., Univ. of Tsukuba) N. Venkatachalam1, Yu Saito1, K. Soga1 and Y. Nagasaki2, “Y2O3 Nanophoshpors Prepared by Enzymatic Decomposition for Upconversion Bioimaging" (2007/10/1-2, Awaji, Hyogo).

tional Symposium on Atomic Technology (Tokyo Univ. of Sci., Anan Col. Tech.*) 5) Second Interna

Yoshihito OKUMURA, Yu SAITO, Kazuaki SHIMIZU, Tomoya KONISHI* and Kohei SOGA "Novel

Fabrication Route for Yittrium Oxide Nanoparticles by Using Phosphate Salt" (2007/10/1-2, Awaji,

Hyogo).

6) B ioceram ics 20 (Tokyo. U niv. of Sci.) K . Soga, Y. Saito, K . Shim izu, R . K unibonu and J.

C hiba “R are-E arth-D oped C eram ic N anophosphors for B ioim aging” (2007/10/24-26, N antes,

F rance).

Deregulated expression of a novel component of TFTC/STAGA histone acetyltransferase

complexes, rat SGF29, in hepatocellular carcinoma: possible implication for the oncogenic

potential of c-Myc Nobuya Kurabe, Yuko Komiya, Seiichi Mochizuki, Kumiko Katagiri, Akinori Sugiyama, Yasushi Kawasaki, Fumio

Tashiro.

Department of Biological Science and Technology, Tokyo University of Science

Department of Biological Science and Technology, Tokyo University of Science