Platzhalter Titelbild Let’s make it work! Efficient biotechnology for efficient animal nutrition Dr. Ralf Kelle December 4, 2013
Platzhalter Titelbild
Let’s make it work!Efficient biotechnology for efficient animal nutrition
Dr. Ralf KelleDecember 4, 2013
Population growthA growing population meansa growing demand for meat, eggs, milk, and fish
Sustainable nutrition Healthy nutrition, with adequate animal protein, that is affordable and available over the long term
Climate
Population
Water
Biodiversity
Resource-efficient animal feeding Limited natural resources must be used as efficiently as possible
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 2
Amino acids are “essential”! Stable trends drive demand
Nutrition
Start-up 2014
Lysine production in Castro
Start-up 2012
Expansion of Midwest Lysine Start-up 2015
Lysine JV in Volgodonsk
Start-up 2013
Agroferm expansion2013-2015
Fermas Technology & Launch Platform
2011-2014
Expansion of biotech R&D
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 3
Biotech investment projects inthe Health & Nutrition Business Unit
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 4
From production organism to production process
T, pH, shear stress, P/V
pO2 airflow
Concentration of S, P, Mg, Na, K, Fe, Zn, Co, etc.
Quality of water and other raw materials
Byproduct concentration
Product concentration
Osmotic pressure
Feed of C-source, N-source and base/acid
Solubility and oxidation of salts and vitamins
InputProduct formation
Byproduct formation
Growth of organism
Product form
Process efficiency
Crystallization
Viscosity
Foaming
Output
Evaporation
Spray granulation
Biolys®
Fermentation
Mother liquor• residual lysine-HCl• decomposition products • impurities from
raw material source
Cell separation
Ion exchange
Evaporation
Fermentation
Dry L-lysine-HCl
Crystallization
Drying
Wastewater
Ammoniumsulphate
Drybiomass
Drying
Liquid L-Lysine base Wastewater
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 5
Biolys® production compared with conventional production
Bacteria + C-source + Ammonia + Sulphuric Acid + Nutrients for Bacteria + Water!
Fructose
Glucose-6-P Fructose-6-P Fructose-1,6-BisP
Fructose-1-PDihydroxyaceton-P
Glycerinaldehyd-3-P
Glycerat-1,3-BisP3-P-Glycerat2-P-GlyceratP-Enolpyruvat
-P
-P-P-P
-P
-P
-P
= O
-P
= O
-P
-P= O
-P= O
Pentosephosphatweg
Phosphotransferase-system (PTS)
glk
ptsIptsHptsM
pgipfkApfk
fdatpi
gap
pgkgpmeno
ppsA
zwf2 devB gnd rpe tal
tkt rpi prsA rbsK pgmA
Pyruvat
= OHOO-
-OOH
AcetylCoA
CitratOxalacetat
AspartatAspartatsemialdehyd
meso-Diaminopimelat
Lysin
Lysin
Homoserin
Threonin
-CoA-CoA
NH3-
-NH3
-OOH
aceElpdlpdA
gltA
acn
icdsucAB
sucCD
fum
mdhmqo
asd lysChom
thrB
dapDdapCdapEdapF
dapAdapB
ddh
lysA
lysIlysG lysE
aceAaceBmez
ppcpck
pyc
Glucose
pykß-Alanin
2-Ketobutyrat
Acetohydroxy-butyrat
Aceto-lactat
Dihydroxymethyl-valerat
Dihydroxy-isovalerat
Isoleucin Valin
Ketomethyl-valerat
Ketoiso-valerat
Pantothenat
panD
coaApanBC
Acetat
poxB
Nicotinat-Biosynthese
Zellwand-Synthese
murE
nadACE
Methionin- S -
metFmetHmetE
metX
ilvBN
ilvC
ilvA
ilvD
ilvE
metBaecD metY
fruK
= O
= O
HOO--OOH-OOH-OOHHOO-
HOO-
-OOH
-OOH
-OOH
= O
-OOH
N
-OOH
= O
-OOH
-OOH
-OOH -OOH
-NH3
NH3 NH3
-NH3
-NH3 sdhABC
ptsIptsHptsF
Lysine
Isoleucine
Isoleucine
Methionine
Valine
Panthotenate
Glutamic Acid
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 6
• Amplify biosynthetic pathways
• Slow down side-reactions
• Avoid feed back inhibition
• Balance precursor supply and side reactions
• Enhance net export into the medium
The art of targeted optimization of metabolism
E. coli & C. glutamicum
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 7
How can speed and efficiency be optimized?
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 8
Good bacterial growth negatively impacts efficiency
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 9
Minimized growth is at the expense of process speed
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 10
Expanded biotech expertise drives progress in productivity
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
1980 1990 2000 2010 2020
Progress, Midwest Lysine2009-2013
Establishment of biotech R&D
Biolys®
production, Fermas1992-2001
Biolys®
production, MWL2000-2008
Mutation and selectionMetabolic engineering and targeted mutation
Functional genomicsSystem biology
Technological development of industrial fermentation= f (yield, product concentration, productivity)
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 11
• Continuous improvement in production costs and process efficiency
• Continuous improvement of lysine content at the same level of value using the other components of Biolys®
• Continuous improvement of product properties for use in feed production
Expanded biotech expertise drives progress in productivity
Packaging
Wastewater treatmentTransportThermal energy
Electricity
Raw materials
Process emissions
Carbon source
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 12
Increase in efficiency of the Biolys®
production process, 2002-2020
Climate warming potential [kg CO2e/kg]
-0.55 -0.51 -0.44-0.01 -0.01 -0.01
0.41 0.36 0.360.01 0.01 0.01
0.470.35 0.34
1.13
0.93 0.84
0.38
0.35 0.34
0.78
0.730.62
-1,0
-0,5
0,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
2002 2008 2020
December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition Page 14
Evonik is working on solutions that go beyond amino acids
Diagnostics for preventing disease and reducing intervention (such as use of antibiotics) in animal nutrition
Probiotics with scientifically proven effect and measurable benefit for use in animal nutrition
Por
tfolio
dev
elop
men
t
Complexity of the technology
Secondary metabolites
Essential fatty acids for aquaculture
L-Valine
L-ThreonineL-Lysine
L-Tryptophan
L-Isoleucine