CoolProducts Organized. Protected. Standardized.
Aug 05, 2015
CoolProductsOrganized. Protected. Standardized.
CoolProducts
CoolBoxTM CoolCellTMCoolRackTM
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Modular tools for cooling, (snap)freezing and
thawing biomedical samples
CoolBoxTM
Ice-free cooling and
freezing
CoolCellTM
Alcohol-free cell
freezing container
CoolRackTM
Modular thermo-
conductive tube and
plate holders
Does this look familiar?
Most researchers still entrust their precious samples to this type of cooling
method
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Here’s the same bucket again….
But in this one, the samples are safely placed in one of our CoolRack modules
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Why are CoolProducts necessary?
� Worldwide effort to develop new standards for
collecting, handling, and processing pre-analytical
samples (blood, tissue, tumor, other biological
specimens or sample materials)� EU: http://www.devicelink.com/ivdt/archive/09/04/003.html
� NCI/NIH: � NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp
� “The lack of standardized, high-quality biospecimens has been widely
recognized as one of the most significant roadblocks to the progress of cancer
research.”
� NIH Office of Biorepositories and Biospecimen Research 2010
Symposium: http://www.brnsymposium.com/meeting/brnsymposium/2010/
� Duke: http://web.genome.duke.edu/cores/biorepository/protocols/
� Molecular Epidemiology Workshop, Shanghai, 2008
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It’s all about the curves….
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….the temperature curves
Each sample goes through the 0oC transition the same way.
Cool, freeze and thaw - identical each and every time
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Consistent curves mean temperature control and experimental
repeatability…..INSTANT STANDARDIZATION
CoolProducts
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CoolBoxTM
Ice-free cooling and
freezing
CoolCellTM
Alcohol-free cell
freezing container
CoolRackTM
Modular thermo-
conductive tube and
plate holders
Versatility- rapidly adapts to any temperature
- can be used for a wide temperature range - from -150oC through +200oC
Consistency & Standardization- uniform temperature profiles for all samples
- inexpensive, comprehensive modular solution-CoolRacks, CoolSinks, CoolBox, CoolTrays
- reduced user error
CoolRack
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Better sample organization
& temperature consistency
- reduced user error
- standardize - assay to assay, user to user, lab to lab, site to site
Organization- indexed & organized sample management
- labels stay dry & legible
- minimize risk of contamination
Patented novel alloy construction
- very rapid thermo-conductivity/adaptivity
Tube holders- some with profile-fit
Plate holders- multi-well & PCR
CoolRack
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- multi-well & PCR
Platforms- rectangular & round
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Modular system is customizable, scalable and versatile
Dry IceLiquid Nitrogen Water Bath
-150oC -78oC 0o to 4oC 37oC +
• Eliminate direct immersion
into LN2
• All tubes are upright
• Same freezing profile for all
• Replaces direct contact with
dry ice
• Eliminates ethanol bath on
the bench – cost savings,
• Gain organization & stability
in ice
• Even temperature distribution
to all samples even as ice melt
• Keeps tubes out of water –
reduced contamination, labels
stay dry
• Consistent thawing profile for
Ice
CoolRack
• Same freezing profile for all
samples
• ThermalTray provides
elevation of samples &
increased distance between
hands and LN2
• Rapidly adapts from ambient
(25oC) to LN2 temp (-150oC) in
12-14 minutes
the bench – cost savings,
reduced hazard & waste
• CoolRack PF provides equal
or faster snap-freezing than
ethanol slurry
• Standardized freezing -
minimized standard deviation in
freeze profiles
• Improved work flow
• Adapts to -78oC in 5-7
minutes
to all samples even as ice melt
• Labels stay dry and legible for
better sample identification
• Reduced risk of sample
contamination
• Rapidly goes from ambient to
<4oC in 90 seconds
•Using ThermalTray, 10-15
hours of <4oC with one pan of
ice
• Consistent thawing profile for
all samples
• All wells at identical
temperature - eliminates “edge
effect” on multi-well plates
• Stable support of tubes &
plates
Temperature control without directly immersing samples
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Cooling - Ice
� Ambient to < 4oC in 60-90 seconds
� 4-6 hours of use when placing CoolRack directly on
ice
� Up to 14 hours cooling with a single pan of ice with
ThermalTray
Cooling rate on ice
CoolRack
Snap-Freezing – Dry Ice
� Equal or better freeze rate than dry
ice/ethanol bath
� No alcohol required – cost savings, reduced
hazard
� Consistent freeze profile for all samples
Freeze rate on dry ice Standard deviation
Cooling rate on ice
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CoolProducts
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CoolBoxTM
Ice-free cooling and
freezing
CoolCellTM
Alcohol-free cell
freezing container
CoolRackTM
Modular thermo-
conductive tube and
plate holders
Ice-free cooling and freezing- 4 hrs w/o lid, 10 hrs with lid (+2oC)
- 3 hrs w/o lid, 6 hrs with lid (-12oC)
Versatile- five temperature options in one box using
cartridges, dry ice or LN2
- portable
- accommodates a variety of tubes & plates
CoolBox
+2oC
Consistent temperature- all wells within 0.1oC regardless of position even
as cartridges thaw
Highly recommended- for highly-sensitive applications such as PCR
and RNA, use in the hood, cleanroom,
vivarium, short transporting, bio-hazardous
containmentPortable ice-free
cooling or freezing
Portable ice-free
cooling or freezing
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CoolBox
-25oC with thermal
regulator pad placed
between CoolRack and
dry ice
Snap-freezing at -78oC
with CoolRack directly on
200cc dry ice
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CoolBox
CoolBox Gel-Filled Container
Temperature range: -150oC through +150oC
Temperature range: Fixed at -20oC or 0oC
Cooling Media:LN2, Dry ice, Wet ice, Cartridge
Cooling Media:Gel
Well-to-well Temperature Variance:0.1oC
Well-to-well Temperature Variance:Up to 4oC
Tube & Plate Formats: Tube & Plate Formats:
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Tube & Plate Formats:0.5ml conical, 1.5ml conical, 1.5-2.0ml cylindrical, 12.5mm dia cryos, mass spec tubes, PCR 96- and 384-well plates, 6-, 12-, 24-, 48-, 96-well plates
Tube & Plate Formats:0.5ml conical, 1.5ml conical, PCR tubes
Cooling Duration:10h (2ºC), 6h (-12ºC), 5h (-25ºC), 6h (-78ºC)
Cooling Duration:8h (0ºC), 3h (-21ºC)
Down Time:None – switch cartridge with another frozen one from the freezer or replenish dry ice, LN2
Down-Time:4-6 hours to re-freeze gel-filled tube rack
CoolBox is more versatile, more consistent
CoolProducts
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CoolBoxTM
Ice-free cooling and
freezing
CoolCellTM
Alcohol-free cell
freezing container
CoolRackTM
Modular thermo-
conductive tube and
plate holders
CoolCell
� Alcohol-free cell freezing� No hassle
� No on-going expense
� No variability
� High reproducibility
� Better cell viability� Better cell viability
� Faster work flow
� CryoPrep System� everything you need for cell prep in the
hood – CoolRack CFT, CoolBox, CoolCell
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It’s all about the curves…..
Fusion time and cooling profiles are highly reproducible from run to run
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Better cell viability
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In primary human cells, CoolCell
significantly outperforms
CoolCell Alcohol-Filled Container
No alcohol •No hazardous fluids•Solid core and insulation design•No pre-cooling required
Requires 250ml alcohol•Replace alcohol every 5 uses (250ml)•Keep track of # of uses•Alcohol degrades slightly each time it’s used•Pre-cool alcohol in refrigerator
Very consistent -1oC/min rate•All vials have uniform freeze rate every time
•Radially symmetric – design enables consistency
Inconsistent freeze rate•Each run is slightly different due to alcohol degradation•Two circles of wells – different freeze rates for each circle
Cost savings ~$338/year maintenance per unit
CoolCell
Cost savings•No maintenance cost•Pays for itself quickly
~$338/year maintenance per unit•Change alcohol weekly (~$6/wk)•Dispose haz waste (~$0.75/wk)•10 units = $3,380 to use EVERY YEAR
Lid opens easily •Not cold to the touch when removing from the -80C freezer
Screw cap very difficult to remove when frozen•Protective glove required when handling frozen unit
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to room temp for re-use
Low impact on freezer•1/3 the heat impact on freezer compared to alcohol-filled units
Large thermal mass impacts local freezer area•Large heat capacity removed from alcohol impacts nearby samples
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Customer quotes
� Stanford University � “We run a registry, in which large amounts of PBMCs are processed for long-term
cyropreservation. After testing the CoolCell out, we found slightly better cell viability (>90%)
than our current cell freezing containers, and there is no Isopropanol waste generated. Overall,
the CoolCell has proven to us to set a new bar in cryopreservation.”
� Merck Pharmaceuticals � “I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a
96well flat bottom CoolBox system. All these will be used for our assays. I need one more 96well flat bottom CoolBox system. All these will be used for our assays. I need one more
product to complete my collection of ice-free cooling devices. I already passed on my evaluation
of the CoolCell and the other groups are pretty excited and are going to use them.“
� UC Berkeley� “Our lab at UC Berkeley recently tried out demo models for the CoolRackM90, CoolSink, and
Thermal Tray and we loved them and thought they were very useful. We were just amazed by
how quickly the Cool Rack chills our tubes within minutes while preventing the possibility of
water getting in and contaminating our samples. ……We decided to get all of them after trying
them out due to their effectiveness. Our lab manager has been looking for a company that was
offering such products and fortunately, we've the right place!”
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Genentech Amgen Pfizer Roche Harvard MITCalTech USCF Glaxo Bristol Myers Squibb Novartis
Translational Genomics Gilead Sciences MerckMedImmune Genpharm Elan Pharmaceuticals
Centocor Boehringer Ingelheim Oncomed NIH/NCITakeda Pharmaceuticals CDC Covance XOMA
Quest Diagnostics Otsuka America PharmaceuticalsSalk Institute Burnham Institute Scripps Institute
Stanford University Yale Genzyme EMD SeronoShire HGT Alnylam Pharmaceuticals Cell-System
Thank you.
Shire HGT Alnylam Pharmaceuticals Cell-SystemJackson Labs Beth Israel Deaconess Hospital HHMI
Whitehead Institute Harvard Systems Biology Exelixis Charles River Labs Sanofi Pasteur Penn StateLawrence Berkeley Lab Batelle UC Berkeley
Cedars Sinai Hospital Cleveland Clinic IDEXX BiogenPerkin Elmer Genetics Blood Systems Research Inst.
BC Cancer Research Center USC Pacific BioscienceUCLA Precision Therapeutics Northwestern
Michelle Nemits
VP Sales & Business Development
Mobile: 415-336-4415
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