The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in Conductive Stereolithographic Scaffold Structures

The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in

Conductive Stereolithographic Scaffold Structures

Suvi Haimi, PhDAdult Stem Cell Group, BioMediTech,

University of Tampere, Finland &

Department of Biomaterials Science and Technology, University of Twente, The Netherlands

BioMediTech

• Tissue engineering and stem cell

technology

• Biomaterials

• Sensor and actuator technologies

• Measurement and imaging technologies

• Biotechnology

• Immunology

• Cancer and mitochondrial research

• Systems biology

• Bioinformatics

• Computational methods in biomedicine

BioMediTech is a joint institute of

University of Tampere & Tampere University of Technology

Over 250 scientists in world-class basic and translational research

Tampere, Finland

Adult Stem Cell Group: world leader in stem cell based reconstructions of cranial bones

Example of Research Excellence

Combining stem cells with

biomaterial

Isolation of stem cells

GMP culture for 2-6 weeksTransplantation

back into same patient

Combination of biomaterials and stem cells differentiated from subcutaneous fat

Harvesting of adipose

tissue

Department of Biomaterials Science and Technology

University of Twente

Enschede, The Netherlands

Stereolithography

• Polymer synthesis

• Polymer engineering

• State-of-the-art scaffold processing techniques

Mechanical stress

CompressionStretch

Fluidflow

+-

Electrical stimulation (ES) in skeletal tissue engineering

Wound healing

Embryonic development

Action potential

• Used in numerous biomedical applications

• Bioactive – can be easily incorporated with negatively charged biomolecules

Electrically conductive polypyrrole (PPy)

J Biomed Mater Res A, 2009

Langmuir, 2013

Tissue Eng Part A, 2013

Ann Biomed Eng, 2014

Biocompatibility of PPy in vitro

Our team was the first to show

• Biocompatibility with ASCs

• Attachment and spreading can be enhanced by charging

J Biomed Mater Res A, 2009

Langmuir, 2013

Charged PPy (+)

Non-Charged PPy (0)

100 µm

100 µm

Biocompatibility of PPy in vitro

My team was the first to show

• Biocompatibility with ASCs

• Attachment and spreading can be enhanced by charging

J Biomed Mater Res A, 2009

Langmuir, 2013

Charged PPy (+)

Uncharged PPy (0)

100 µm

100 µm

• Charging increased cell surface area of ASCs at 3h

PPy promotes smooth muscle differentiation

Björninen et al. submitted

Calponin/DAPI MHC/DAPI SMA/DAPI

Aim

Differentiate hASCs towards skeletal tissues applying ES via designed poly (trimethylene

carbonate) (PTMC) scaffolds coated with PPy

Study design

Electroactive polypyrrole coating

3D electrical stimulation

Designed 3D scaffolds

Adipose stem cells

Control ES 7d - rest 7d

ES 4h/d ES 8h/d0

1

2

3

4

5

6

7

α-SMA

Calponin

MHC

Re

lati

ve

ex

pre

ss

ion

at

14

d

/Electrical stimulation in 2D- Effects on smooth muscle differentiation

*

*

control ES 4h/d ES 8h/d0.00

0.50

1.00

1.50

2.00

2.50

α-SMASM22αMHC

Re

lati

ve

ex

pre

ss

ion

at

14

d

Electrical stimulation in 3D-Effects on smooth muscle differentiation

Electrical stimulation in 3D enhanced ASC osteogenic differentiation

*

*

14

d

ControlES

14 d

Summary

• PPy-coated scaffolds are potential for skeletal tissue engineering

• ES is potential method to engineer muscle and bone tissue

• Screening of more effective ES parameters in 3D environment needed

11.12.2008

Acknowledgements

TAMPERE UNIVERSITY OF TECHNOLOGY

Funding• Academy of Finland

• TEKES, Finnish Funding Agency for Technology and Innovation

• Pirkanmaa Hospital District Competitive research funding, EVO

• The City of Tampere

Adult Stem Cell GroupDepartment of Biomaterials Science and Technology

Key collaborators•

• Tampere University of Technology, Finland• VTT Technical Research Center,

Finland

Kiitos / Thank You!