The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in Conductive Stereolithographic Scaffold Structures Suvi Haimi, PhD Adult Stem Cell Group, BioMediTech, University of Tampere, Finland & Department of Biomaterials Science and Technology, University of Twente, The Netherlands
17
Embed
The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in Conductive Stereolithographic Scaffold Structures
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in
Conductive Stereolithographic Scaffold Structures
Suvi Haimi, PhDAdult Stem Cell Group, BioMediTech,
University of Tampere, Finland &
Department of Biomaterials Science and Technology, University of Twente, The Netherlands
BioMediTech
• Tissue engineering and stem cell
technology
• Biomaterials
• Sensor and actuator technologies
• Measurement and imaging technologies
• Biotechnology
• Immunology
• Cancer and mitochondrial research
• Systems biology
• Bioinformatics
• Computational methods in biomedicine
BioMediTech is a joint institute of
University of Tampere & Tampere University of Technology
Over 250 scientists in world-class basic and translational research
Tampere, Finland
Adult Stem Cell Group: world leader in stem cell based reconstructions of cranial bones
Example of Research Excellence
Combining stem cells with
biomaterial
Isolation of stem cells
GMP culture for 2-6 weeksTransplantation
back into same patient
Combination of biomaterials and stem cells differentiated from subcutaneous fat
Harvesting of adipose
tissue
Department of Biomaterials Science and Technology
University of Twente
Enschede, The Netherlands
Stereolithography
• Polymer synthesis
• Polymer engineering
• State-of-the-art scaffold processing techniques
Mechanical stress
CompressionStretch
Fluidflow
+-
Electrical stimulation (ES) in skeletal tissue engineering
Wound healing
Embryonic development
Action potential
• Used in numerous biomedical applications
• Bioactive – can be easily incorporated with negatively charged biomolecules
Electrically conductive polypyrrole (PPy)
J Biomed Mater Res A, 2009
Langmuir, 2013
Tissue Eng Part A, 2013
Ann Biomed Eng, 2014
Biocompatibility of PPy in vitro
Our team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Non-Charged PPy (0)
100 µm
100 µm
Biocompatibility of PPy in vitro
My team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Uncharged PPy (0)
100 µm
100 µm
• Charging increased cell surface area of ASCs at 3h
PPy promotes smooth muscle differentiation
Björninen et al. submitted
Calponin/DAPI MHC/DAPI SMA/DAPI
Aim
Differentiate hASCs towards skeletal tissues applying ES via designed poly (trimethylene