Supplemental file 1

A B C

Cx40 expression (red) in the aorta of Cx40fl/fl (A), TIE2-Cre Cx40fl/fl (B), and Renin-Cre Cx40fl/fl (C) mice. Nuclei are stained using Sydow green showing endothelial as well as smooth muscle nuclei perpendicular to each other. Endothelial cell membranes are decorated by Cx40 which was completely abolished in TIE2-Cre Cx40 fl/fl mice demonstrating successful deletion of Cx40 in endothelial cells. Bars indicate 20 µm. Images are representative for 3 experiments in each genotype.

Supplemental file 1