Selecting the Right WAX Column - agilent.com Column... · Agilent J&W WAX GC columns deliver excellent inertness with greater sensitivity than competitive WAX columns. With the increasing
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Do you need an extended temperature range to analyze high
molecular weight analytes?
Keep up the good work.
Keep up the good work.DB-HeavyWAX DB-WAX
Ultra Inert
DB-HeavyWAX for high molecular weight analytes
– Higher max temp limit: 280 °C isothermal and 290 °C programmed
– Shorter run times
www.agilent.com/chem/db-heavywax
DB-WAX Ultra Inert for polar compounds
– Withstands repeated temperature cycling to upper limits without sacrificing peak shape performance
– Identical selectivity to DB-WAX
www.agilent.com/chem/dbwaxui
DB-FATWAX Ultra Inert for FAMEs and free fatty acids
– Single column analysis
– Solvent rinseable and tolerates aqueous injections
www.agilent.com/chem/fatwax-ui
2x10
- 0.1- 0.05
00.05
0.10.15
0.20.25
0.30.35
0.40.45
0.50.55
0.60.65
0.70.75
0.80.85
0.90.95
11.05
2x10
- 0.1- 0.05
00.05
0.10.15
0.20.25
0.30.35
0.40.45
0.50.55
0.60.65
0.70.75
0.80.85
0.90.95
11.05
Counts (%) vs. Acquisition Time (min)
3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69
Acquisition Time (min)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
C9
C10C18C16
C14C12
C20 C22 C24C26
C28
C30C32
C34 C36
C9
C10
C18C16C14
C12C20 C22 C24C26 C28
C30C32
C34 C36
Final temp 280 °C
Final temp 250 °C1
2, 3
4
5
6 78
9
2 3 4 5 61
2 3 4 5 61
1
2
34
56 7
8
9
Competitor WAX column30m x 0.25 mm, 0.25 μm
1
2, 3
4
5
6 78
9
2 3 4 5 61
2 3 4 5 61
1
2
34
56 7
8
9
Agilent DB-FATWAX Ultra Inert 30m x 0.25 mm, 0.25 μm
Analysis of Cold Pressed Pink Grapefruit Essential Oil
1. Acetic acid2. Propionic acid3. Isobutyric acid4. Butyric acid5. Isovaleric acid6. Valeric acid7. 4-Methylvaleric acid8. Hexanoic acid9. Heptanoic acid
2x10
- 0.1- 0.05
00.05
0.10.15
0.20.25
0.30.35
0.40.45
0.50.55
0.60.65
0.70.75
0.80.85
0.90.95
11.05
2x10
- 0.1- 0.05
00.05
0.10.15
0.20.25
0.30.35
0.40.45
0.50.55
0.60.65
0.70.75
0.80.85
0.90.95
11.05
Counts (%) vs. Acquisition Time (min)
3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69
Acquisition Time (min)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
C9
C10C18C16
C14C12
C20 C22 C24C26
C28
C30C32
C34 C36
C9
C10
C18C16C14
C12C20 C22 C24C26 C28
C30C32
C34 C36
DB-HeavyWAXRun time: 13 minFinal temp: 280 °C Ramp: 30 °C/min Flow: 5 mL/min
DB-WaxRun time: 19 minFinal temp: 250 °C Ramp: 30 °C/min Flow: 5 mL/min
Superior inertness, unique selectivity, and thermal robustness for separation of FAMEs and underivatized free fatty acids.
High temperature capability of Agilent J&W DB-HeavyWAX enables shorter run times and sharper peaks for high molecular weight, high boiling compounds without the risk of carry-over.
This information is subject to change without notice.
© Agilent Technologies, Inc. 2018 Published in the USA, June 29, 2018 5991-9415EN
Is this a new or existing method?
Existing New
Yes
Yes Yes
No FAMEs and/or free fatty acids
NoNo
DB-FATWAX Ultra Inert
VF-WAX MS
DB-WAX Ultra InertDB-HeavyWAX
All other analytes*
All other detectors
Mass spectrometry
What is your maximum operating temperature?
> 250 °C < 250 °C
Even after 50 hours of exposure at the upper temperature range, the DB-WAX UI GC column demonstrated superior inertness and stability with minimal peak shifting.
2.98
pAXOf fset : 0 YOf fset : 22
28272625242322212019181716151413121110987654321
5048464442403836343230282624222018161412108642
DB-WAX UI after 50h at 250 ºCDB-WAX UI after 1h at 250 ºC
1
23.93
34.28
45.74
57.36
69.29
711.16
813.25
914.55
1016.54
1120.69
1225.35
1.46
1
23.96
34.33
45.85
57.39
69.48
711.27
813.52
914.73
1016.59
1120.99
1225.88
1.46
RT [min]
Inertness of Agilent DB-WAX UI
272625242322212019181716151413121110987654321
525048464442403836343230282624222018161412108642
1.34
2.59
RT [min]
pA
23.41
34.33
44.89
56.30
68.09
79.44
811.79
912.08
1014.07
1119.37 12
22.73
1
XOffset : 0 YOffset : 23
Competitor Standard WAX after 50h at 250 ºCCompetitor Standard WAX after 1h at 250 ºC
1
23.42
34.04
44.94
56.29
79.45
811.91
912.12
1014.02
1118.55 12
22.14
68.17
Inertness of competitor WAX column
Injector: 250 °C, Split 1:75Injection volume: 1 µLFlow rate: 1.1 mL/min, H2 gas Oven program: 130 °C Isothermal Detector: FID @ 260 °C
1. 2-Nonanone2. Decanal3. Propionic acid4. Ethylene glycol5. Heptadecane6. Aniline7. Methyl dodecanoate8. 2-Chlorophenol9. 1-Undecanol10. Nonadecane11. 2-Ethylhexanoic acid12. Ethyl maltol
Are you satisfied with the sensitivity and reproducibility of your analysis and frequency of column changes?
Are you using DB-WAX Ultra Inert? What compounds are you analyzing?
What detector are you using?
42 42.5 43 43.5 44 44.5 45 45.5 46 46.5 47 47.5 48 48.5 49 49.5 50 50.5 51 51.5 52 52.5 53 53.5 54 54.5 55 55.5 56 56.5 57 57.5 58 58.5 59 59.5 60 60.5 61 61.5 62 62.5 63 63.5 64 64.5 65
Initial Injection
Repeat Injection
Meranzin
Isomeranzin
Osthole
*Specialty columnsStrong acids DB-FFAPBasic analytes and amines CAMAlcohols and glycols in wine/spirits CP-WAX 57CB
Selecting the Right WAX ColumnAgilent J&W WAX GC columns deliver excellent inertness with greater sensitivity than competitive WAX columns. With the increasing number of available Agilent J&W WAX columns, it can be challenging deciding which one is best suited for your needs.
This poster will help you select the right WAX column for optimal performance, based on factors such as application analyte mix and the type of detector being used.
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