Do you need an extended temperature range to analyze high molecular weight analytes? Keep up the good work. Keep up the good work. DB-HeavyWAX DB-WAX Ultra Inert DB-HeavyWAX for high molecular weight analytes – Higher max temp limit: 280 °C isothermal and 290 °C programmed – Shorter run times www.agilent.com/chem/db-heavywax DB-WAX Ultra Inert for polar compounds – Withstands repeated temperature cycling to upper limits without sacrificing peak shape performance – Identical selectivity to DB-WAX www.agilent.com/chem/dbwaxui DB-FATWAX Ultra Inert for FAMEs and free fatty acids – Single column analysis – Solvent rinseable and tolerates aqueous injections www.agilent.com/chem/fatwax-ui 2 x10 - 0.1 - 0.05 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55 0.6 0.65 0.7 0.75 0.8 0.85 0.9 0.95 1 1.05 2 x10 - 0.1 - 0.05 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55 0.6 0.65 0.7 0.75 0.8 0.85 0.9 0.95 1 1.05 Counts (%) vs. Acquisition Time (min) 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 Final temp 280 °C Final temp 250 °C 1 2, 3 4 5 6 7 8 9 2 3 4 5 6 1 Competitor WAX column 30m x 0.25 mm, 0.25 μm 2 3 4 5 6 1 1 2 3 4 5 6 7 8 9 Agilent DB-FATWAX Ultra Inert 30m x 0.25 mm, 0.25 μm Analysis of Cold Pressed Pink Grapefruit Essential Oil 1. Acetic acid 2. Propionic acid 3. Isobutyric acid 4. Butyric acid 5. Isovaleric acid 6. Valeric acid 7. 4-Methylvaleric acid 8. Hexanoic acid 9. Heptanoic acid Acquisition Time (min) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 C9 C10 C18 C16 C14 C12 C20 C22 C24 C26 C28 C30 C32 C34 C36 C9 C10 C18 C16 C14 C12 C20 C22 C24 C26 C28 C30 C32 C34 C36 DB-HeavyWAX Run time: 13 min Final temp: 280 °C Ramp: 30 °C/min Flow: 5 mL/min DB-Wax Run time: 19 min Final temp: 250 °C Ramp: 30 °C/min Flow: 5 mL/min Superior inertness, unique selectivity, and thermal robustness for separation of FAMEs and underivatized free fatty acids. High temperature capability of Agilent J&W DB-HeavyWAX enables shorter run times and sharper peaks for high molecular weight, high boiling compounds without the risk of carry-over. This information is subject to change without notice. © Agilent Technologies, Inc. 2018 Published in the USA, June 29, 2018 5991-9415EN Is this a new or existing method? Existing New Yes Yes Yes No FAMEs and/or free fatty acids No No DB-FATWAX Ultra Inert VF-WAX MS DB-WAX Ultra Inert DB-HeavyWAX All other analytes* All other detectors Mass spectrometry What is your maximum operating temperature? > 250 °C < 250 °C Even after 50 hours of exposure at the upper temperature range, the DB-WAX UI GC column demonstrated superior inertness and stability with minimal peak shifting. 2.98 pA XOf f set : 0 YOffset : 22 28 27 26 25 24 23 22 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 50 48 46 44 42 40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 DB-WAX UI after 50h at 250 ºC DB-WAX UI after 1h at 250 ºC 1 2 3.93 3 4.28 4 5.74 5 7.36 6 9.29 7 11.16 8 13.25 9 14.55 10 16.54 11 20.69 12 25.35 1.46 1 2 3.96 3 4.33 4 5.85 5 7.39 6 9.48 7 11.27 8 13.52 9 14.73 10 16.59 11 20.99 12 25.88 1.46 RT [min] Inertness of Agilent DB-WAX UI 27 26 25 24 23 22 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 52 50 48 46 44 42 40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 1.34 2.59 RT [min] pA 2 3.41 3 4.33 4 4.89 5 6.30 6 8.09 7 9.44 8 11.79 9 12.08 10 14.07 11 19.37 12 22.73 1 XOffset : 0 YOffset : 23 Competitor Standard WAX after 50h at 250 ºC Competitor Standard WAX after 1h at 250 ºC 1 2 3.42 3 4.04 4 4.94 5 6.29 7 9.45 8 11.91 9 12.12 10 14.02 11 18.55 12 22.14 6 8.17 Inertness of competitor WAX column Injector: 250 °C, Split 1:75 Injection volume: 1 µL Flow rate: 1.1 mL/min, H2 gas Oven program: 130 °C Isothermal Detector: FID @ 260 °C 1. 2-Nonanone 2. Decanal 3. Propionic acid 4. Ethylene glycol 5. Heptadecane 6. Aniline 7. Methyl dodecanoate 8. 2-Chlorophenol 9. 1-Undecanol 10. Nonadecane 11. 2-Ethylhexanoic acid 12. Ethyl maltol Are you satisfied with the sensitivity and reproducibility of your analysis and frequency of column changes? Are you using DB-WAX Ultra Inert? What compounds are you analyzing? What detector are you using? 42 42.5 43 43.5 44 44.5 45 45.5 46 46.5 47 47.5 48 48.5 49 49.5 50 50.5 51 51.5 52 52.5 53 53.5 54 54.5 55 55.5 56 56.5 57 57.5 58 58.5 59 59.5 60 60.5 61 61.5 62 62.5 63 63.5 64 64.5 65 Initial Injection Repeat Injection Meranzin Isomeranzin Osthole *Specialty columns Strong acids DB-FFAP Basic analytes and amines CAM Alcohols and glycols in wine/spirits CP-WAX 57CB Selecting the Right WAX Column Agilent J&W WAX GC columns deliver excellent inertness with greater sensitivity than competitive WAX columns. With the increasing number of available Agilent J&W WAX columns, it can be challenging deciding which one is best suited for your needs. This poster will help you select the right WAX column for optimal performance, based on factors such as application analyte mix and the type of detector being used.