NORMANDEAU ASSOCIATES, INC. EPA Region 5 Records Ctr ...
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NORMANDEAU ASSOCIATES, INC. EPA Region 5 Records Ctr. ^ ' *3450 Schuylki/l RoadSpring City, PA 19475-1124(610)948-4700 237584(610)948-4 752 (Fax)www.normandeau.com
23 July 2004
J U L 2 6 ^ ' >Mr. Russ CepkoCBS CorporationBloomington ProjectGateway Center11 Stanwix Street, Room 628Pittsburgh, Pennsylvania 15222-1384
SUBJECT: Fish and Crayfish Tissue Sampling for Aroclor and Lipid Analysis at StoutsCreek near Bennett's DumpNormandeau Project No. 20103.000
Dear Russ:
Normandeau Associates has completed fish and crayfish sampling from three locations in Stout'sCreek (Monroe County) near Bloomington. Indiana. Sampling was conducted to obtain fish tissuesamples from three locations near Bennett's Dump, and crayfish samples from one location. Thesamples were shipped via Federal Express to En Chem Labs in Green Bay, Wisconson and AXYSAnalytical Services in Sidney, British Columbia for PCB (Aroclor or Congener as applicable) andlipids analysis. Copies of all data sheets and the Chain-of-Custody forms are enclosed.
Fish were collected using a pram electrofishing unit consisting of hand-held anodes and a pram-mounted cathode (McLaren/Hart 1996). Direct current was supplied from a gas-po\veredgenerator set at 230 volts with an output of approximately 5 amperes, depending upon theconductivity of the water at each sample location. Sample locations were as follows:
Station Location
BD-i Stout's Creek, Monroe Co., near West Hunter Road, downstream from Bennett'sDump (upstream section)
BD-2 Stout's Creek, Monroe Co., near Acuff Road (middle section)
BD-3 Stout's Creek, Monroe Co., near its confluence with Beanblossom Creek (MapieGrove Road)
Targeted fish species were creek chub (Semotilus atromaculctus), an omnivore: Green sunfish(Lepomis cyanellus) or longear sunfish (Lepomis megaloiis), pelagic water column feeders; andwhite sucker (Catostomus commersoni), a bottom feeder The field effort was designed to collectfish and crayfish as follows (Viacom, 2004):
Bedford, NH, Corporate
Norfolk. CT Hanover. MA Haverstraw. NY Aiken. SCLewes. DE Hampton. NH Drumore. PA Stevenson, WAYarmouth. ME Westmoreland. NH Spring City, PA
An Employee-owned Company r1^
NORMANDEAU ASSOCIATES, INC.
Station BD-1 6 green sunfish between 20 and 40 grams for 6 whole body Aroclor analyses3 green sunfish between 20 and 40 grams for 1 whole body Congener analysis3 green sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs greater than 20 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers greater than 30 grams for 6 whole body Aroclor analyses3 white suckers greater than 30 grams for 1 whole body Congener analysis
enough crayfish greater than 5 grams each to produce 3 composite samples forwhole body Aroclor analysis
Station BD-2 6 green sunfish greater than 20 grams for 6 whole body Aroclor analyses3 green sunfish greater then 20 grams for 1 whole body Congener analysis3 green sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs between 25 and 55 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers greater than 30 grams for 6 whole body Aroclor analyses3 white suckers greater than 30 grams for 1 whole body Congener analysis
Station BD-3 6 longear sunfish between 20 and 50 grams for 6 whole body Aroclor analyses3 longear sunfish between 20 and 50 grams for 1 whole body Congener analysis3 longear sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs greater than 20 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers between 30 and 50 grams for 6 whole body Aroclor analyses3 white suckers between 30 and 50 grams for 1 whole body Congener analysis
Stream sections of several hundred meters were shocked at each station until sufficient fish werecollected to meet the study specifications.
Captured fish and crayfish were placed in aerated buckets for 24 hours, prior to sampleproduction, to purge their gut contents; or their gastrointestinal tracts were removed. Wirescreening was attached to the inside of the buckets to allow fecal material to pass through andprevent reingestion.
The fish samples analyzed for PCB Aroclors consisted of single individuals. Fish samplesanalyzed for Congeners were composites of 3 individuals each. All fish and crayfish samplescontained at least 20 grams of tissue. All replicate analyses were conducted at the respectivelaboratories. Split samples were submitted in the field as requested to Ms. Regina Bergner(Tetra Tech), who provided oversight on behalf of US EPA, and analyzed by US EPA'scontract laboratory.
Each sample and an equipment blank, were given a two-digit project identification code (BD forBennett's Dump) and a four-digit sequence number. Sample numbers were not repeated.
NORMANDEAU ASSOCIATES, INC.
The length/weight regressions are augmented with data derived from scale samples taken fromtarget species. Scales were removed from two fish per species at each station: the longest one anda second of intermediate length. The age of each fish was determined by counting the number ofannuli, and the length of each fish following each year's growth (Murphy and Willis 1996),calculated from measurements of scale radius according to the formula:
Ln = (Lc - a / Sr * Sn) + a where:
Ln = the length at annulus (the end of each year's growth)Lc = the length at captureSr = the scale radiusSn = the radius to annulus na = the y-intercept of a regression of body length scale radii for 18 fish of the species
captured from Stouts Creek
Lastly, the stomach contents of 10 to 15 fish for each target species, at each station, were purgedand the invertebrates contained within identified and counted. Samples were produced either byforcing water through the stomach of each fish with a syringe to collect back-washed food itemsin a pan or by removal of the stomach contents by dissection. Food items were then compositedby species and sample station, preserved in alcohol, and transported to our lab for analysis.Invertebrates were identified to either genus of the lowest taxonomic level possible given their ageand condition.
Results
A total of 25 species (Table 1) was collected from Stouts Creek (all stations combined), indicatinga moderately rich fish community. Two additional species (tadpole madtom and shortheadredhorse), collected in 1998 but not in 2004, increases the total to 27. The dominant species atone or more stations were minnows: creek chub, central stoneroller, or bluntnose minnow.
At Station BD-1, 733 fish but only 11 species were collected. A community density of 31,912fish per hectare (ha) and 228.8 kilograms per hectare (Table 2), was consistent with what wouldbe expected from a stream flowing through karst topography, where productivity is typically high.Most common were creek chubs (19,156/ha, 156.3 kg/ha) that represented 60.0 percent of thenumerical total. Green sunfish 7,906/ha, 52.4 kg/ha) represented an additional 21.8 percent ofthe total. All other species comprised less than 10 percent.
At BD-1. length-frequency histograms were assembled for green sunfish and creek chubs (Figure1). An insufficient number of white suckers (the third target species) was collected to produce ameaningful histogram. Results showed that the majority of fish within these two species were toosmall to be considered harvestable by most anglers. Only 12 of 104 (11.5 pet.) green sunfishmeasured exceeded 100 millimeters (approx. 4 inches) and all were less than 150 millimeters(approx. 6 inches). Only 4 of 100 (4.0 pet.) creek chubs exceeded 150 millimeters. Using theselengths as a basis, the density of green sunfish and creek chubs most likely to be harvested at BD-1 was low. Application of the percentage of fish longer than 100 or 150 millimeters to therespective population estimates produced values of 22 harvestable green sunfish and 19harvestable creek chubs per 100-meters of stream channel.
NOHMANDEAU ASSOCIATES, INC.
At Station BD-2 channel width and surface area increased; hence, the fish community was not asconfined as was it was upstream. Increased stream gradient produced swifter current velocities;and the substrate composition changed. The streambed consisted mostly of cobble/gravel, wheresand/silt was predominant at BD-1. A species-poor community was also observed at BD-2 where10 species, and 902 fish, were collected. Community density decreased to 18,976 fish and 91.7kilograms per hectare. The dominance hierarchy changed relative to that observed upstream ascentral stoneroller became the most abundant species. Stonerollers comprised 37.5 percent( 7 , I l l / h a , 24.0 kg/ha) of the cumulative total. Co-dominant were creek chubs, a larger-sizedspecies, that represented 35.3 percent (6,768/ha, 48.7 kg/ha) of the total. Third in abundancewas blacknose dace, 13.2 percent (2,537/ha, 8.2 kg/ha).
At BD-2, length-frequency histograms were produced for green sunfish and creek chubs (Figure2). No green sunfish measured exceeded 100 millimeters. Only 2 of 100 (2.0 pet.) creek chubsexceeded 150 millimeters. Thus, the density of green sunfish and creek chubs most likely to beharvested at BD-2 was again low, 0 and 7 fish per 100 meters.
At Station BD-3 current velocity decreased and the streambed changed back to one consistingmostly of sand/silt. Community richness, near the confluence of Stouts Creek with BeanblossomCreek, was nearly twice that observed upstream. Twenty-three species were collected.However, community density further decreased to 12,089 fish and 57.1 kilograms per hectare.The dominance hierarchy changed again, as bluntnose minnow became the most abundantspecies. Bluntnose minnows represented 48.5 percent (5,872/ha, 11.7 kg/ha) of the total,followed by creek chub at 18.7 percent (2,300/ha, 8.4 kg/ha), and central stoneroller at 9.5percent (1,152/ha, 3.7 kg/ha).
Length-frequency histograms were assembled for all four target species (Figure 3). Eleven of 25(44.0 pet) green sunfish and 9 of 38 (23.7 pet.) longear sunfish measured exceeded 100millimeters. Because the population estimates for these panfish were relatively low, calculationsof the number of each species longer than 100 millimeters remained low also. The number oflarger green sunfish per 100 meters of stream channel was 11 and the number of larger longearsunfish was 9. Only 1 of 104 (1.0 pet.) creek chubs and no white suckers exceeded 150millimeters. Estimates for the number of larger fish for these species were 1 and 0 per 100meters, respectively.
Note that larger green sunfish (BD-2) and white suckers (BD-1 and 2) were collected for tissueanalysis but very few or none were found in the 100-meter stream sections established for thepopulation estimates.
Length/Weight relationships from Stouts Creek (all stations for 1998 and 2004 combined) areshown for each target species as Figures 4 through 7. The regression equations shown on thefigures can be used to calculate the weight (y variable) for any fish within the range of lengths (xvariable) encompassed by the fish submitted for analysis. For each species applicable ranges are:
Green Sunfish 90 - 155 mm Creek Chub 110-240 mmLongear sunfish 95 - 155 mm White Sucker 130-290 mm
NORMANDEAU ASSOCIATES, INC.
A total of 72 fish and 3 crayfish samples were collected from the three stations and submitted foranalysis. A Chain-of-Custody form was included noting: the identity of the fish used for eachsample, the sample type (whole body or fillet), whether the sample consisted of a single individualor was a composite, and the sample weight.
Samples were submitted in accordance with the QAPjP Plan pertaining to this project with oneexception. Three white suckers used to produce the composite sample for Congener analysis atStation BD-3 exceeded the 30 to 50 gram size range. They were between 79 and 92 grams.
Ecological Study
To obtain information regarding species composition, population density, size distribution oftarget species, length/weight relationship of target species, and the food items selected by targetspecies, quantitative samples were collected at each station.
Methods
The electrofisher was used to produce species composition and population estimates fromrepresentative 100-meter stream sections that encompassed at least one run-riffle-pool habitatsequence at each station. A tape was used to delineate this length, and a series of 10 widthmeasurements were averaged to calculate the respective surface areas sampled (in hectares: 1hectare = 2.47 acres). Depletion data from three consecutive capture efforts at each station wereregressed to produce population estimates for each species and for all species combined (VanDeventer and Plaits 1983). Source data included the total length and weight of each fish and thecumulative weight of all fish collected for each species. Results were calculated as the number offish per 100-meters of stream channel, and the number of fish and biomass per hectare. Anyadditional species collected from the greater shocking effort employed to obtain target fish fortissue analysis, but not present in the 100-meter stream sections used for the depletion estimates,were noted.
Length-frequency distribution, in 5-millimeter increments (by station) for the four target speciesare shown as a series of histograms. Each histogram displays the data for the first 100 fish(approx.) measured from each species. If fewer than 100 individuals were captured fromparticular species, all were included. Note that lengths were taken for non-target species andlength-frequency distributions can be derived for those as well.
The length/weight relationship for each target species (all stations combined) is shown as a seriesof regression plots. Data used to produce the plots were taken from fish submitted for tissueanalysis. To provide enough data to produce meaningful plots, lengths and weights recordedfrom a nearly identical collection effort at the same three stations in 1998 were pooled with thesedata from 2004 Plots are given for both natural log (In: base e) transformed and untransformeddata.
NORMANDEAU ASSOCIATES, INC.
As an example: the formula: y = 3.375x - 12.418, from Figure 5a for the two longear sunfishlengths shown on Table 3 produces the following weights:
Length In length In weight Calc.wt. Meas. wt.
105mm 4.65396 3.89115 27 grams 28 grams155mm 5.04343 4.60356 99 grams 87 grams
The calculated weight of 27 grams, for the 105-millimeter longear, compares well with theweight measured in the field of 28 grams (see Table 3). For the 155-millimeter fish, thecalculated weight of 99 grams differs from the field measurement by 12 grams, showing thatweights of individual fish of equal length can vary. Regression equations average this intra-specific variance. The most accurate results will be obtained by using log (natural log: base e)transformed length data. Correlation coefficients (R2) for the regressions shown on figures 4a,5a, 6a, and 7a range between 0.84 and 0.96 so the formulas are accurate.
Table 3 provides length calculations for target species following each year of growth, referred toas mean length at annulus. That is, the analysis correlates age and length. These results aresummarized as follows:
Mean Length at Annulus (mm)Species Year 1 Year 2 Year 3 Year 4 Year 5
Creek Chub 54 98 129 163 172White Sucker 111 148 209 268Green Sunfish 74 89 104 122 133Longear sunfish 60 79 106 121 152
The results of the stomach contents analysis are given in Table 4. The analysis was interesting inthat it showed a considerable degree of resource partitioning between the different species. Creekchubs, the omnivore, were feeding mostly on aquatic earthworms (Lumbriculidae) and crane flieswithin the genus Tipula; relatively large-sized invertebrates found in depositional habitats.Stomach contents from the bottom feeding white suckers contained smaller sized items, mostlymidges (several genera in the family Chironomidae) and a crustacean in the genus Lirceus, bothfound in depositional areas. Resource partitioning was also indicated from the contents of the twopelagic feeding sunfish species. The diet of green sunfish consisted mostly of Lirceus andLumbriculidae, while chironomids were the predominant food item found in the longear sunfishstomachs.
The diet of all target species was comprised mostly of forms adapted to depositional environments(Merritt and Cummins 1996) where fine sediments and paniculate organic matter accumulate, andthe potential for sorption of PCB molecules is relatively high.
Sincerely, L
George M. Christian(Project Biologist)
NORMANDEAU ASSOCIATES, INC.
References:
Merritt R. W. and K. W. Cummins, editors. 1996. An introduction to the aquatic insects ofNorth America, 3rd edition. Kendall/Hunt Publishing Company, Dubuque, Iowa.
McLaren/Hart, 1996. Field procedure no. 23: Protocol for evaluating fish populations andsampling fish tissue. ChemRisk (a Division of McLaren/Hart), Portland, Maine, 4October 1996.
Murphy, B. R., and D. W. Willis, editors. 1996. Fisheries techniques, 2nd edition. AmericanFisheries Society, Bethesda, Maryland.
Normandeau Associates, Inc. 1998. Fish and crayfish tissue sampling for aroclor and lipidAnalysis near Neal's Landfill and Bennett"t Dump (letter report). Normandeau (ProjectNumber 17750.000), Spring City, Pennsylvania, 6 May 1998.
Van Deventer, J. S., and W. S. Platts. 1983. Sampling and estimating fish populations fromstreams. Transactions of the North American Wildlife and Natural Resources Conference48: 349-354.
Viacom, Inc. 2004. QAPjP, Volume XXXIX: Bennett's Dump, Stout's Creek fish, water, andsediments sampling and analysis plan. Viacom. Pittsburgh, Pennsylvania, Spring 2004.
Table 1. Fish Collected from Stouts Creek, Monroe County, Indiana near Bennetts Dump.
Sample Date:Gear:
Scientific Name
AmiidaeAmia calva
CyprinidaeCampostoma anomalumCyprinus carpioLuxilus chtysocephalusLythrurus umbratilisNotropis buccatusNotropis hudsoniusPimephales notatusRhinichthys atratulusSemotilus atromaculatus
CatostomidaeCatostomus commersoniErimyzon oblongusHypentelium nigricans
IctaluridaeAmeiurus natalis
PoeciliidaeGambusia holbrooki
CentrarchidaeAmbloplites rupestrisLepomis cyanellusLepomis macrochirusLepomis megalotisLepomis hybridMicropterus salmoides
PercidaeEtheostoma caeruleumEtheostoma flabellareEtheostoma nigrumPercina carbonaria
25-27 May 2004Electrofisher (barge unit: three pass depletion estimate)
BD-1Common Name Number Percent
bowfin
central stoneroller 40 5.5%common carpstriped shiner Predfin shiner
silverjaw minnowspottail shiner P
bluntnose minnow 7 1.0%blacknose dace 55 7.5%
creek chub 440 60.0%
white sucker 3 0.4%creekchub sucker
Northern hog sucker
yellow bullhead
Eastern mosquito fish
rock bassgreen sunfish 160 21.8%
bluegill sunfish Plongear sunfishhybrid sunfish
largemouth bass
rainbow darter 10 1.4%fantail darter 18 2.5%johnny darter
logperch
Total Fish for Three Passes Combined 733 1 00.0%Duration for Pass Number 1 (minutes) 55 min.Section Length (meters)Area Sampled (hectares)
Total Species/Hybrids (stationCatch Rate for Pass Number 1
100m0.0244 h
total*) 11(fish/hr.) 518
BD-2Number Percent
338 37.5%
18 2.0%1 19 13.2%318 35.3%
3 0.3%
P
35 3.9%
P
14 1.6%57 6.3%
902 100.0%44 min.100m
0.0495 h
10850
BD-3Number Percent
P
54 9.5%PP16 2.8%3 0.5%6 1.1%
275 48.5%1 0.2%
106 18.7%
14 2.5%5 0.9%
P
4 0.7%
1 0.2%25 4.4%1 0.2%
38 6.7%4 0.7%
1 0.2%2 0.4%8 1.4%3 0.5%
567 100.0%38 min.100m
0.0495 h
23536
Water Quality:
Water Temp. (C)Dissolved Oxygen (mg/1)Conductivity (micro-siemans/cm @ KT)Specific Conductance (micro-siemans/cm @ K25)
21.08.4485525
20.56.4560613
Note:
1) The total number of species captured at each station was derived by adding any additional species from the shockingeffort expended to collect fish for tissue analysis to those from the population estimate. These are denotedwith a P (present) but are not quantified. Two additional species (tadpole madtom and shorthead redhorse) werecollected from Stouts Creek in 1998 but not in 2004.
Table 2. Population estimates calculated from fish data collected from Stouts Creek during 25 - 27 May 2004.Estimates are presented for each species and for all species combined.
Area (Hectares):
Est.
779
Species:
creek chub 467central stoneroller 42blacknose dace 84bluntnose minnow 7fantail darter 79rainbow darter 13white sucker 3green sunfish 193longear sunfishmosquitofishhybrid sunfishjohnny dartercreek chubsuckersilverjaw minnowlogperchspottail shinerredfin shinerbluegillrock bass
Sum (QA/QC)
0.0244
BD1no./ha
Pooled
31912
By Species
19156174134262853233518132
7906
36398
kg/ha
228.8
156.39.79.23.22.70.75.652.4
239.8
0.0495
BD2Est no./ha
Pooled
939 18976
By Species
335 6768352 7111126 253720 40182 165818 3594 7439 783
19690
kg/ha
91.7
48.724.08.20.62.20.54.65.2
94.1
Est.
598
114571
291211526404485331121101
0.0495
BD3no./ha
Pooled
12089
By Species
2300115220
587247203035258088184162926154
21842220220
12443
kg/ha
57.1
8.43.70.011.70.11.00.511.114.60.10.40.20.30.20.54.71.21.60.1
60.5
Figure 1.Length Frequency Distribution of Green Sunfish at North
Hunter Road (Station BD #1)I Green Sunfish
Length (mm)
Length Frequency Distribution of Creek Chubs at NorthHunter Road (Station BD #1)
w -|2 -Ta•n 10 -
"O Q
£o 6i_•Q A. -Ez 2
0 _
10
• Creek Chub
12
6 6
.1ill
! S 5! ° g> r— oo
Total N = 100
6
4 4 •
n n n 1,11Illlllll UNI | 0 0 | 0 | 0 0 0 0 0 0 0 0 0 | |
3 S S S S S 5 S
Length (mm)
Figure 2.Length Frequency Distribution of Green Sunfish at Acuff
Road (Station BD #2)
D Green Sunfish
Length (mm)
Length Frequency Distribution of Creek Chubs at Acuff Road(Station BD #2)
on
• Cree
181ft
(0"a 16•o 14 _
« 17 -
- 100 8
1 2-i-1 2T
o-t
tChub
Total N = 100
13
8 8
Iu 6 *\ • 4 4 ^t riIII. III!
^ ^ C S ^ " ® ^ ' " ® ^ " * ' * 5 * " ® ^ 'm < D ( y h - r * - c o « o a j a > o O T -
' S S S ^ K S ^ ^ ^ g S S
4
I 21 • 1 1 1 10 I . 0 , 0 0 0 0 0
• • • • • • •? s a s s ? ? s s s s s s si / > o i n ^ > i 6 d i i O ( 6 u S ( 6 i f > o i / k ( ^T - c M c v j c o o 5 ^ - ^ r i n i n < 5 i D ^ r * . o B
Length (mm)
Figure 3.Length Frequency Distribution of Green Sunfish at Maple
Grove Road (Station BD #3)
D Green Sunfish
Total N = 25
Length (mm)
Length Frequency Distribution of Creek Chubs at MapleGrove Road (Station BD #3)
-inMra OK3 £.3
'"O
>t- 15 -o 13
X 10 -4
E3 cz ° H
0 _
• Cree
2°
17
3 3
.11
16 17
kChub
TV-it oi M — -i ne1 Otai N — 1 Uo
1 10
il0 1 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
• • • • •t ' ^ ' f f t ' ^ ' O t ^ o t ' ^ ' t n ^ f O t ' ^ ' C f t ^ ' O> T - ^ - < N j c M O ( O ^ - ^ - t f > u > « ) 2 > h * h - .
! S ! 8 e R § S i ^ | ^ 6 . p g ^ ^ . A 6 . A 6 . A ^ . A 6 ^
Length (mm)
Figure 3 (cont).Length Frequency Distribution of White Sucker at Maple
Grove Road (Station BD #3)
7
1 632 5
1 4-
•5 3i_S o
Z 1
D White Sucker
Total N = 146
5
2
I 1
0 0 0 0
s s $ $ s $
0 0 0
S 8 S& £ 8Length (mm)
0 0 0 0I I i i
£ £ £ s i S
8stm
•o
= 4
I 2z
0
Length Frequency Distribution of Longear Sunfish at MapleGrove Road (Station BD #3)
D Longear Sunfish
6 6
I
4
2
7Total N = 38
2 2 2
1 1 1 1 1 1
£Length (mm)
4.5
2.5
Figure 4a.Green Sunfish Length/Weight Relationship (Log base e transformed)
from Stouts Creek
|y = 3.2039x-11.84R2 = 0.8403
4.5 4.6 4.7 4.8 4.9
In (length in millimeters)
Green Sunfish
• Linear (Green Sunfish)
5.1
100
90
80
70
w 60
I01= 50
I)
^ 40
30
20
10
Figure 4b.Green Sunfish length/Weight Relationship (Untransformed)
from Stouts Creek.
y = 1.0326x - 87.754
R2 = 0.7422Green Sunfish
•Linear (Green Sunfish)
90 100 110 120 130
length in millimeters
140 150
Figure 5a.Longear Sunfish Length/Weight Relationship (Log base e transformed)
from Stouts Creek.J -
4 C
4 -go>.££O)
3
2 C
Longear Sunfish
Linear (Longear Sunfish)
4.5 4.6 4.7 4.8 4.9 5 5.1
In (length in millimeters)
90
80
70
60
E 50O)
40
30
20
10
85
Figure 5b.Longear Sunfish Length/Weight Relationship (Untransformed)
from Stouts Creek.
95 105 115 125
length in millimeters
135 145 155
Longear Sunfish
Linear (Longear Sunfish)
4.9
4.4
O)
,E£
3.9
3.4
2.9
4.7
Figure 6a.Creek Chub Length/Weight Relationship (Log base e transformed)
from Stouts Creek.
y = 2.8322x-10.456
R2 = 0.8931
4.8 4.9 5 5.1 5.2
In (length in millimeters)
5.3 5.4 5.5
Creek Chub
•Linear (Creek Chub)
200
180
160
140
M 120
5o>.S 100
s>95 80
60
40
20
100
Figure 6b.Creek Chub Length/Weight Relationship (Untransformed)
from Stouts Creek.
y=1.0678x-113.81
R2 = 0.8535Creek Chub
•Linear (Creek Chub)
120 140 160 180
length in millimeters
200 220 240
6
5.5
in
5en
4.5
3.5
Figure 7a.White Sucker Length/Weight Relationship (Log base e transformed)
from Stouts Creek.
y = 2.9835x-11.322R2 = 0.9555 White Sucker
Linear (White Sucker)
4.8 4.9 5 5.1 5.2 5.3 5.4 5.5
In (length in millimeters)
5.6 5.7
260
10
Figure 7b.White Sucker Length/Weight Relationship (Untransformed)
from Stouts Creek.
130 150 190 210 230
length in millimeters
White Sucker
Linear (White Sucker)
250 270 290
Table 3. Age and Growth Relationships Calculated from Scale Samples Collected fromStouts Creek, Monroe County, Indiana on 25 - 27 May 2004.
Station
BD-1BD-1BD-2BD-2BD-3BD-3
BD-1BD-1BD-2BD-2BD-3BD-3
BD-1BD-1BD-2BD-2
BD-3BD-3
underlined
Species
creek chubcreek chubcreek chubcreek chubcreek chubcreek chub
white suckerwhite suckerwhite suckerwhite suckerwhite suckerwhite sucker
green sunfishgreen sunfishgreen sunfishgreen sunfish
longear sunfishlongear sunfish
Length
158124130235135175
145196163282142267
101138113153
105155
Weight Age
5723251713175
mean length
339646o32203
mean length
21482180
mean length
2887
mean length
rvranIVrvV
Yearclass
200020012002200020001999
at annulus
IIIInIVi
IV
200320012002200020032000
at annulus
rvVnirv
2000199920012000
at annulus
rvV
20001999
at annulus
Length (mm) atI
424190763936
54
133921268812899
111
68677587
74
5863
60
II
1091011301096874
98
122163153
153
148
798890101
89
7287
79
III
132124
166109116
129
166
234
225
209
91104108114
104
93120
106
annulusIV
158
212135146
163
268
267
268
98121
146
122
103138
121
V
172
172
133
133
152
152
= annulus judged to be at scale margin
Table 4. Stomach Contents of Fish Collected from Stouts Creek, Monroe County, Indiana near Bennetts Dump.
Sample Date:Gear:
Taxon:
Creek Chub:
Terrestrials
ChironominiChironomusDugesiaGammaridaeLirceusLumbriculidaeOrconectesTabanusTanytarsiniTipula
White Sucker:
CeratopogonidaeChironominiChironomusCopepodaEphydridaeLirceusMicrotendipesOptioservusOstracodaPhaenopsectraProcladiusSphaeriidaeSphaeromiasTanypodinae
Tanytarsini
Sunfish:
Terrestrials
Fish Tissue
CheumatopsycheChironominiChironomusDubiraphiaErpobdellidaeGammaridaeLirceusLumbriculidaeMicrotendipesOrconectesPolvpedilumProcladiusTanypodinae
TanytarsiniTipula
25-27 May 2004Electrofisher
CommonName
1 7-yr cicadamillipede
midgemidge
flatwormsideswimmer
sowbugearthworm
crayfishhorse fly
midgecrane fly
biting midgemidgemidge
fish liceshore flysowbugmidge
riffle beetleseed shrimp
midgemidge
fingernail clambiting midge
midge
midge
1 7-yr cicadacaterpillar
minnow
caddisflymidgemidge
riffle beetleleech
sideswimmersowbug
earthwormmidge
crayfishmidgemidgemidge
midgecrane fly
Habitat
.-
lentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional1 enti c/loti c-depositionallentic/lotic-depositionallentic/lotic-depositional
lentic-erosional/depositionallotic-erosional/depositional
lotic/lentic-erosional/depositional
lentic/lotic-depositionallentic/lotic-depositional
lentic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional
lotic-eorsionallentic-depositionallentic-depositional
lentic/lotic-depositionallentic/lotic-depositional
lentic-depositionallotic/lentic-
erosional/depositionallotic-erosional/depositional
--
-
lotic-eorsionallentic/lotic-depositionallentic/lotic-depositional
lentic/lotic-ersionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional
lentic-depositionallentic/lotic-depositional
lotic/lentic-erosional/depositional
lotic-erosional/depositionallotic/lentic-
erosional/depositional
BDNumber
31
60
1
39
12
11811
1
1
3
1
1
1
2383
3
13
-1Percent
2.9%1.0%
57.7%
1.0%
37.5%
5.9%1 1 .8%
5.9%5.9%
47.1%5.9%5.9%
5.9%
5.9%
BD-ZNumber Percent
2 2.6%1 1.3%3 3.8%1 1.3%1 1.3%
65 83.3%3 3.8%
1 1.3%1 1 .3%
147 68.4%25 11 .6%5 2.3%
8 3.7%10 4.7%
3 1.4%10 4.7%3 1 .4%
3 1.4%
1 0.5%
Green
2.5%
0.8%
0.8%
0.8%
19.3%69.7%
2.5%
0.8%2.5%
7 16.7%33 78.6%
1 2.4%1 2.4%
BD-3Number Percent
1 14.3%
6 85.7%
2 22.2%4 44.4%
1 11.1%
2 22.2%
Longear
5 8.1%1 1.6%
1 1 .6%
3 4.8%12 19.4%17 27.4%1 1.6%
1 1 .6%1 1.6%2 3.2%7 1 1 .3%
6 9.7%1 1.6%4 6.5%
NORMANDEAUASSOCIATES
GENERAL FISHERIES INFORMATION SHEETf*^1i#»nf" / /• /? ' ' & ' *•
Job NumbeLocation (CLocation (SSample DatGear Type:Purpose of
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NORMANDEAU ASSOCIATES
GENERAL FISHERIES INFORMATION SHEET
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NORMANDEAU ASSOCIA TES
GENERAL FISHERIES INFORMATION^ ^ t f A M ^ V 1 / t ft? / / ?^^IICQU \tf<'fff^-l ' f-3 (WHfit fail
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NORMANDEA U ASSOCIA FES
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GENERAL FISHERIES INFORMATION SHEETClient >Job Number:Location (General):,Location (Specifier. &) - ?Sample Date: AS. M /tftyGear Type: '£,0 A A^Purpose of Sample /f# A*
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QA byPage / of 2on
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NORMANDEAU ASSOCIA TES
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NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: _
Address: //3450Schuylkill RoadSprincLCJl uEA 19475-1124
JiO) 948-^
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(6"10) 9 -ATOO) Fax: (610)948-4752Phone:
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Samples Were:1 . ,8rTfpp*bd or hand delivered 2^CTiifi5tijOr ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels andJCO£ record?
NoteS^ ~— Note's- Yes No Yes No Yes No s^' . '\'--^
COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample / / /7 / \Yes No Yes No Yes No Yes No ( ^ I
NORMANDEAU ASSOCIATES, INC.
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COC Tape Was: 1. Present on outer packageYes No
3. Received broken / leakingYes No
2. Unbroken on outer packageYes No
Whito r~r~, DMr~
4. Received within holding timesYes No
5. Discrepancies between sample labels and COC record?Yes No
3. Present on sampleYes No
4. Unbroken on sampleYes No
NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY
3450 Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752
Client:
Address:
Phone:
Samplers:Project No.:
f/ > W/ M f'C '</ Client Contact:
Sample Return To Client [ ]
Disposal Disposal by Lab [ ]
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SAMPLE TEMP.IN AT
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Acute
£"»£ +<Tr*/
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COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample S r~\ ff ~*~ *\Yes No Yes No Yes No Yes No ( ff ' 1
NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: £ A.-.V Client Contact:
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Ad3450 Schuylkill RoadSpring City, PA 19475-1124610)948-4700 • Fax: (610)948-4752
SanProje
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3, i /flat Q*J/'
fr
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Non- Hazard [ ] Flammable [ ] Unknown [ ]
Relinquished
Special
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Acute
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Samples Were:
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COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample / -*y / —7 AYes No Yes No Yes No Yes No 1 /) t )
NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: i t f f rv>^
3450 Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752
Address:
Phone:
Samplers:
Project No.:
A Client Contact:
SampleDisposal
Return To ClientDisposal by Lab
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Date:
/2c^>
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'Matrix Codes:S - soil GW - ground waterSE - sediment ST - stormwaterSL - sludge PW - processed waterO - oil DW - dilution waterWW - waste water RW - receiving water \SW - surface water X - other^" //JETA*
Samples Were:1. ^higJB^d or hand delivered 2. phFjlgja or ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels and COC record?
Notes: Notes: Yes No Yes No Yes No .—-"" >^ __
COC Tape Was: 1 . Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample /^ s/ J7 <~y \Yes No Yes No Yes No Yes No ( ( 9i ' J
NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY
Ad3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752
SanProje
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'Matrix Codes:- soil GW - ground water- sediment ST - stormwater- sludge PW - processed water- oil DW - dilution water- waste water RW - receiving water \- surface water X - other^ ft^svj^ v
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NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: J)S i
3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752
Address:
Phone:
Samplers:
Project No.:
Client Contact:
Sample Return To Client [ ]
Disposal Disposal by Lab [ ]
•
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*Matrix Codes:S - soil GW - ground waterSE - sediment ST - stormwaterSL - sludge PW - processed waterO - oil DW - dilution waterWW - waste water RW - receiving waterSW - surface water X - other
Samples Were:1. , Shipped or hand delivered 2. (CRiDedor ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels and COC record?
'Notes^ N6Te<r' Yes No Yes No Yes No ^._ — ^COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample ./ / ff \
Yes No Yes No Yes No Yes No f £,£ "7 )
NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client:
3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752
Address:
Phone:
Samplers:
Project No.:
Client Contact:
Sample
Disposal
Return To Client [ ]
Disposal by Lab [ ]
•
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SampleTime
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*Matrix Codes: £fi?l'ifc^S - soil GW - ground>taterSE - sediment ST - stormwaterSL - sludge PW - processed waterO - oil DW - -d^^44w)n waterWW - waste water RW - receiving waterSW - surface water X - other
Samples Were:t ~ShJ£gj|!'d or hand delivered 2.>-eri'frteekor ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels and_COC record7
"Notes: StoWS: Yes No Yes No Yes No s^S~~~~'~^\COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample / r?x/>~7 \
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FISH SAMPLING -KEELD DATA SHEET (FRONT)page / of 2
STREAM NAME <?r LOCATION A///**'STATION * / RB'rRMILx STREAM CLASS
LA LONG RIVER BASIN
STORET # AGENCY
2EIESURVEV
GEAR INVESTIGATORS—iKiASONFOlJ SURVEYFORM COMPETED BY DATE
TIME
SAMPLECOLLECTION
HABITAT TYPES
GENERALCOMMENTS
How were tht fish captured? Dbackpaci: Unfits barge
Block nets used? D YEE QHSfT
Sampling Ituration Start timf o<1ff End time /o^>
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- 7-7
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FISE SAMPLING -KEELD DATA SHEET (ERONTjpage of
STREAM NAME
STATION f 6 b * / RJArERMILr
LAT LONG
STORE! #
GEAR
FORM COMPLETED BY
LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE !&bM REASON FOE. SURVEYTIME AW PM
SAMPLE .COLLECTION
tfo.0* /
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GENERALCOMMENTS
How were the fish capmred? D back pad; D tois barge
Block nets used? DYES DNO
Sampling Duration Start iim: End time
D other
Duration
Stream width fin meters) Ma:: Mean
Indicate the percentage of each habitat type presentD Riffle: % D Poolr % D Rum % D STISESD SubmerpeiJ Macrophvtcs % D Other ( )
• %%
OPTIONAL: LENGTH (mm)AVElGHT (gj(25 SPECIMEN MAX SUBSAMPLEj
kavid Bioassessmeni Protocols For use in Stream; ana Wadeabic River: Pcnpnyior, benihic
FISH SAMPLING -FIELD DATA SHEET (FRONT)page / of
STREAM NAME
STATION* B t > * | RTVSRMILE
LAT LONG
STORET *
GEAR
FORM COMPLETED BY
| LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE f-TS-od REASON FOR SURVEYTIME AM PM / U/7> s^Y
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How were the fish capmred? D backpack Q tote barge Q other
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Stream width (in meters^ Ma:: Mean
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OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX STJBSAMPLE)
For Use in Sireams and Wadeabic Rivjr.1:. Pennhvior. Benth
FISH SAMPLING-FIELD DATA SHEET (FRONT)page / of /
STREAM NAME j LOCATION
STATION * tfD * 1 PJVSRMILE
LAT LONG
STREAM CLASS
RIVER BASIN
STORET* | AGENCY
GEAR
FORM COMPLETED BY
INVESTIGATORS
DATE -77 W REASON FOR SURVEY
TIME AW PM
SAMPLECOLLECTION
fc*>#3
HABITAT TYPES
GENERALCOMMENTS
How were the fish captured? D back pad; D IOK barge
Block nets used? D YES D NO
Sampling Duration Start tame / J 3 & End time d to
.Stream width (in meters) Ma:: Mean
Indicate the percentage of each habitat Type presentQ Riffles % D Pools % D Rims % D SnagsD Submerged Macrorjbvtes % D Other ( \
Q other
I)uratioD 4 O
%%
OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX SUBSAMPLEj
6lick.»<>* Ebc*. | iq. J- 62 I 71
HI
5leM?/tlU/ 3 ?* Jtl
FISH SAMPLING-FIELD DATA SHEET (FRONT)of
STREAM NAME LOCATION
STATION*/?/? 2 RJVERMILE STREAM CLASS
LA LONG RJVERBASIJO
STORET *
GEAR
FORM COMPLETED BY
AGENCY
INA'ESTJGATORS
DATETIME
7MREASON FOE.
jLSAMPLECOLLECTION
HABITAT TYPES
GENERALCOMMENTS
How were ths fish capm red? D back pad:
Block nets used? D YES BffO
Sampling Duration Start twit End
Stream width (in meters] Ma:: 3e3 -
Ekfe faarpt D other
nmt Duration * ' Mit} _ ,. j.- , A /v 6',^'
. , ^?/ c/ £ /) ,-.Mian •fg>s» T < 7 « f r r /J\f(i^^dS>
Indicate the percentage of each habitat type presentD Riffles % D Pools % D Jtuns % D Snaes - %D Submersed Macropiivtss % D Othir
Cl") f2 ^i^ if' $TJ
( } % . / f
v9' ^/^/f^ ~ /«?,>,
OPTIONAL: LENGTH (mm)AVEIGHT(25 SPECIMEN MAX SUBSAMPLE)
Rapid Bioassexsmeni Protocols For Use m Sireams and Wadeabic River:. Pcnphywr,, henimc
FISH SAMPLING-MELD DATA SHEET (FRONT)pa?e £ of
STREAM NAME SVtu/r £>,
STATION * &D ' 1 RTVERMILE_
LAT LONG
STORET #
GEAR
FORM COMPLETED BY
LOCATION
STREAM CLASS
RIVER BASIN
AGENCY £/r F>>/
INVESTIGATORS
DATE S'-lA'0/ REASON FOR SURVEYTIME AM PM /) <? r/-
/'ci'7 £1 T' /
SAMPLECOLLECTION
« - > » '
HABITAT TYPES
GENERALCOMMENTS
How were tht fish capture
Block nets used? D YE
Sampling Duration Start
Stream width fin meters)
d? Dbaclcpack D tore bares Q other
£ DNO
dm: End rime Duration </</*'/<-'
Ma;' Mean
Indicate the percentage of each habitat type presentD Riffle: % D Pool.': % D ruins % D Snaps %D Submerged Macropnvtss % D Other 1 ) %
SPECIES TOTAL(COUNT)
OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX SUBSAMPLE)
37
131
Ji.
<i>0
5V
Rapid Biocssessmem prowcois Fo:- Use in Sireams and Wadeabh River:. Pciiphyior,, beminc
FISH SAMPLING -PTELD DATA SHEET (FRONT)
STREAM NAME S-Leub Cfrefc.
STATION* 8D-3. RJVERMELE
LAT LONG
STORET*
GEAR
FORM COMPLETED BY
| LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE £~-S&c^ SEASON ran SURVEYTIME <&' AM PM /) J?_// c$ cy
SAMPLECOLLECTION
/? uM * ^
HABITAT TYPES
GENERALCOMMENTS
How were the fish captured?
Block nee used? D YES
Sampling Duration Start cm:
D back pack Diotebarpt D other
Q NO
/??! Endnmt /fir6 Duranon £'§*, ^
Srream width (in meters) Ma:: Meaii
Indicate the percentage of eachD Rifiiec % D Pool?D Submerges Macrophytss
habitat type present% D Rims % D Snaes • %
% D Other ( ) %
*
OPTIONAL: LENGTH (mm)AVEIGET (g)5 SPECIMEN MAX SUBSAMPLEj
FISH SAMPLING-FIELD DATA SHEET (FRONT)I of /
STREAM NAME S^ouJrS Q fQL\i
STATIONS' BD-3- RTVERMILE
LAT LONG
STORET*
GEAR
FORM COMPLETED BY
! LOCATION
STREAM CLASS
RIVER. BASIN
AGENCY
INVESTIGATORS
DATE f •£?-<••<•? REASON FOR SURVEY-
TIME ^1t>' AM PM
SAMPLECOLLECTION
(?^*3
HABITAT TYPES
GENERALCOMMENTS
How were the fish captured?
Block nets used? D YES
Sampling Duration Start nm:
D back pack D tott bargt D other
DNO
(46<l End time A? ? 7 Duration "21? ^
Stream width (in meters') MM: Mean
Indicate the percentage of eachD Riffles % D PoolsD Submerged Macrophvtes
habitat type present% QJttms % DSTiatK • %
% D Other ( 1 %
OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX SUBSAMPLEj
i' I'rnmm/c rnr Tivr in Sirp.nm': nn/! IrVnripnnii ' Rivr- Pfnn'mi
FISH SAMPLING -FIELD DATA SHEET (FRONT)of
STREAM NAME <T£t>/r C^JC
STATION * &D - 3 RJVERMILE
LAT LONG
STORET #
GEAR
FORM COMPLETED BY ^c:
LOCATION J^uo^ A*u.>
STREAM CLASS $c.j/, £r*u?
RIVER BASIN /&,,, yf^ajt*.
AGENCT ^/^ £?rf
INVESTIGATORS S&C . ^). //IT. £**DATE ^-^o/ REASON Fonsufe-TIME - (^ PM /? &>J_ /T,1 X "fpJ. <L.S'- / / I-3S~C*J ^oi</)'' • i
SAMPLECOLLECTION
/,„*/
HABITAT TYPES
GENERALCOMMENTS
How were the fish capture
Block nets used? D YI
Sampling Duration Start
Stream width (in meters)
d? D bade pad; BfoiebarEt D other
, BrfT ^^
nm: ^O."Jo End tims //-'» ^ -Durntipn- -? « /*/»*
Ma:: ^ Mear, ^ f?^ Kf ^^^'^(/Fe
Indicate thepercentage of each habitat typt presentCrfdfflK -i % Bl^olr ^ % B-RUTIE £ % D Snaus • %D Submerged MacrDpbvteE % D Other ( 1 %
»,<$<* .' m ,3 r* n fg tf to) n A/ - foou.ft1) /c ^) /y #) ^ , » ^y//.^ /& to /a ^^> f7 >/*» « y^ /ifeOPTIONAL: LENGTH (mmWEJGHT (g)
(25 SPECIMEN MA3: SUBS AMPLE)
FISH SAMPLING-HELD DATA SHEET (FRONT)of ?
STREAM NAME 5-yWfs C'tefc
STATION * 5O-.3 RJVERMD-I
] .AT LONG
STORET*
GEAR
FORM COMPLETED BY
LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE f-l+O"! REASON FOE. SURVEY
TIME AM PM
SAMPLECOLLECTION
fa*l
HABITAT TYPES
GENERALCOMMENTS
How were the fish captured? D back pack D tois barge D other
Block nets used? D YES D NO
Sampling Duration Sfflrttjrns End nrnt DuratiDT)
Stream width On meters') Mas: Meari
Indicate the percentage of each habitat type presentD Riffles % D Pools % D Rims % D Snaes %D Submerged Macroribvtss % D Other ( ) %
G»-kt»r 4/v{ A'fy f>oM fi&0 jb*f »t-Mf ff i iflKliss l*s*e
OPTIONAL: LENGTH (mm)nVEIGHT (g)SPEOMEN MAX SUBSAMPLEl
Rauid Bioassessmenl Protocols' For Use in Sireams and Wadeabic River: Pennhvior..
FISH SAMPLING-FIELD DATA SHEET (FRONT)pa^e ^ of J>
STREAM NAME S46«.h C'ctk-
STATI OK! £ B 5 ' 3 RA/ERMILE
LAT LONG
STORE! *
GEAR
FORM COMPLETED BY
LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE f-36~~o*l SEASON FOX SURVEY
TIME AM PM
SAMPLECOLLECTION
fa* 1
HABITAT TYPES
GENERALCOMMENTS
Bow were the fish captured?
Block nets used? D YES
Sampling Duration Start cm:
D back pack D tote bargt
DNO
End tirat
D other
Duration
Stream width On meters) Ma:: Mean
Indicate the percentage of eachD RiSlEE % D PoolsD Submersed MacTOtibvtes
1
habitat type present% D Rims % D Snaps
% D Other ! )• %
%
S te»-=£--£*a*vfe r <7i 7 f- l^tafy/'5p&&rt ,
Scv&k chr&f
OPTIONAL: LENGTH (mm)AVElGHT ig)(25 SPECIMEN MAX SUBSAMPLE)
Ravul Bioassessmeni Protocols For Use in Sirecnns and Wadeabic River: Periphywr,, benlhic
FISH SAMPLING FIELD DATA SHEET (FRONT)of
STREAM NAME S),* & Orfe k LOCATION $ev*>ft-{j b~r^f>
STATION* 5 D-J3 RJVERMILE | STREAM CLASS
LAT LONG
STORET #
GEAR
FORM COMPLETED BY ( m C
RIVER BASIN tf^o 0lou*<r>
AGENCY (J15 FP&
INVESTIGATORS bmC
DATE S'g'jrtTIME "*£ AM u0
y HP I^Cy ^,,w».REASON 7DP>. SURVF1'
/ftf3 Esl 1 TiSSut *<* ~>fi^j
SAMPLECOLLECTION
$^* <?
HABITAT TYPES
GENERALCOMMENTS
How were tnt fish captured? D back pad; &tbtt bargt D other
Block nets used? D YEE IBI^O x/J
Sampling Duration Siarttmt IZ<^ End tims /3.'3^ Duration T^A'/t^
Stream width (in meters) Ma:: ' / ' Mean O
Indicate the percentage of each habitat typt presentGMOffles JT % Ea^oolr ^a % »BTlims 5 % D Snags • %D Submerged MacrcmbvteE % D Other i 1 %
OPTIONAL: LENGTH (mm)AVElGHT (gj(25 SPECIMEN MAX SUBSAMPLEl
-ir>r,vve«nriPni Llrr>in'~nh rnr I)ar. in Sireams and Waafiahip. Kiwr.r f-rrrnirvjm-
FISH SAMPLING -FIELD DATA SHEET (FRONT)of
STREAM NAME 54om}s (Ve ^
STATION* fiD-3 RJVERMILE
LAT LONG
STORET #
GEAR
FORM COMPLETED BY IT\
F"
LOCATION
STREAM CLASS
RIVER BASIN
AGENCY
INVESTIGATORS
DATE S-'tt-rf BEASON FDR SURVEY
TIME ~JT Q™ DJ-). £ty/7> c ^
SAMPLECOLLECTION
l f U / * 3
HABITAT TYPES
GENERALCOMMENTS
How were the fish captured?
Block nets used? D YES
Sampling Duration Start time
D bade pack Q-TBie barge D other
QH^T
t < \ ' - 6 t " Endmnt /'I 2i Duration (~> <^>\^
Stream width On meters] Max Mean
Indicate the percentage of eachD Riffles % D PoolsD Submerged Macropiivtes
habitat typt present% D Huns % D Snags • %
% D Other f 1 %
OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX SUBSAMPLEj
w fl 1/Jt'nfian n (/> f-.'T\if
JFISH SAMPLING -FIELD DATA SHEET (FRONT)of
STREAM NAME jvf^ /r /\cd-
STATION £ k b -3 RJVERMELE
LAT LONG
STORST#
GEAR
FORM COMPLETED BY
| LOCATION
STREAM CLASS
RTVER BASIN
AGENCY
INVESTIGATORS
DATE S^£-cSTIME ^If AM PM
REASON POU SURVFi1
A ?ySAMPLECOLLECTION
IU ^3
HABITAT TYPES
GENERALCOMMENTS
How were tht fish captured?
Block nets used? DYES
Sampling Imration Start nm:
D back pad;
D NO
Stream width On meters) Maj:
Indicate the percentage of eachD Riffle: % D PoolsD Submerps'd Macrophvtes
D TDK bargt D othei
End time DuratQL /?*•">•
Mean
habitat type present% DRuns % O Snaps • %
% D Other f ) %
OPTIONAL: LENGTH (mm).'WEIGET (gj(25 SPECIMEN MAX SDBSAMPLE)
Rjnnr?r.-?-mfni rnr Use in Sireams and Wadeabic Rivcrr PwinhvTm-
PRELIMINARY SCAUE READIMG SHEET y -
COLUCTIOW DATE ^RIVER wets*SPECIES
I- READER _£
2^ READER, _
/ OF
DATE
DKTE
RSH
IB LEK6TH RftOtUS
mm
1C m 1ST 30LSCftLERftOlU*.
AGE1 I FwL
U>IW*»J'
REMARKS
/SB XL 7 7" 20OO
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3637
c
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5/7 I
PRELIMINARY SCALE READIMQ SHEET
COLLECTION DATER l V E R METER
SPECIES
I- READER2^ READER
PAGE / OF /
DATE
DATE
RSH
ID
.1urr
SCflLE
RMXOS
ANU. Kt^UREMEMTS SCW.e , mm, )
IE mMEANSCftLE
AGE2 |FmAL
Kn ^A.u-
4-1
9.1 21
ni 75 /- s' Z if ->
17
PRELIMINARY SCALE REAWMQ SHEET
COLLECTION DATE
RIVER &;#;• ME^ 5fan£0'3SPECIES
1S-T
2^ READER
PAGE / OP /
DATE
DATE
RSH
UH6TH WT
SCftLC
fUttos
AMU. (e.«a«> , mm,
nt is- :i 3ZLSCALERADIUS
AGE1 Z FmAi
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( OC
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{,2-
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INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: [J,iafV^ R/CA*,,^ h-» Job #/Task/Labor Code: 2^/^3,^ /
Location:" 5^U/S ^ - /Q***//* jQ»».p .Sample JD: /g/p * / .- ^Coll. Date: y-gp-^ ; ID Time Budget:
Prelim. Sorter: /flffC Split Sorter: Presort ID Time: /^ Date:Subsample: Q-ffone D Abundant Taxa: Split: Split ID Time: —• Date: - Py:
-DRBPIII: D100 D 200 D 300 (Spec, subsamp.) Chiro. ID Time: Date: — By:" . ' . . .,(__ of grids) : :QG.Info: D Sort QC,and/orDJD.QC
: Q Other: :ID QC Time: _____ Date:' ' By:
QC. Taxonomic , Total Presort SplitCheck Order Taxon "Number = Number 4 Number Notes <m.=.aottQCH«ibir)
16
20
21 .22_
23 .24 .25_
26 .27 _
28 _
29
30
31
32 _
33
34
35
•3
/)/t*r.^~-*r • /Q ' ^ _^ . /^L,,r.
* /ASaS
Comments.
Client:,Location:_
Coll. Date:'
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET
v£LJ,*~P
•77
Prelim. Sorter: Split Sorter:Subsample: GMfone D Abundant Taxa: Split:
D RBP III: D 100 D 200 D 300 (Spec. Subsamp.)( of grids)
: D Other:
Job #/Task/Labor Code:' :SampleID: ^
ID Time Budget: •£.£>Presort ID Time: /,g Date^
Split ID Time: -Chiro. ID Time:
en
Date; -Date:
:QG Info: D Sort QC,and/or D ID QC• ID QC Time: Date:' By:
QC TaxonomicCheck Order Taxon
Total Presort Split'Number = Number + Number Notes Qnc. Sort QCHuntw)
-7— / f) A1 / r/<r/«^r r~/e/9fC/V/ 1 1 J F I'A'ar*i /it •
Z 3<&" <<r uJ^Lrr^
E
6_
7_
B_
S_
10_
11
/ yivi>/ rorr/>? /
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if
14
20 _
21
22
23
24
26 _
27 _
2B_
29 _
30 _
31 .32 .33 .
34
35
Comments:/) I
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient:
Location:Col!. Date:
\l [
Prelim. Sorter: Split Sorter:Split:Subsample: £h None D Abundant Taxa:
Q RBP 111: D 100 D 2QD D 300 (Spec. Subsamp.)
: • • • • : : • • •;. \( :-of grids) ;toother:
Job #/Task/Labor Code:Sample ID: fc£) -^
ID Time Budget:Presort ID Time: / . p Date^
Split ID Time:Chiro. ID Time:
DateDate
:'QG Info: -D Sort QC, and/or DID QCQCTime: Date:'
By:By:'By:,
By:
QC .• TaxonomicCheck Order Taxon
Total Presort Split-Number = Number + Number Notes {Inc. Son QC Numbtri
3
4
5•
6_
7
8
9
10
11
14
15•
16
18
19•
20_
21 _
22.-23_
24 _
25_
26
27 _
28 .29 .30 _
31 _
32
33
34
35
Comments:
Page:. of /
Client:location:
Coll. Date:
Prelim. Sorter:
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET_ Job #/Task/Labor Code:_ Sample JD:
ID Time Budget: ^2. oPresort ID Time: /,$-""
_ Split ID Time:Chiro. ID Time:
Split Sorter: _Subsample: Q-ffone D Abundant Taxa: Split:
QRBPIII: D100 D200 D 300 (Spec.Subsamp.)- (__ of grids)
D Other:
Date:QC.Info: D SortQC.and/or D ID QC
ID QC Time: Date:' By:
QC.: TaxonomicCheck Order Taxon
Total Presort Split'Number = Number 4 Number Notes <\ne. Sort QCNumbvl
2
3
A
B
B
7
B
B
10
Llr
t .'
13
14
15
16
17
t gu
19
20_
21 _
22_
23_
24 _
25 _
26 _
27 .28 _
29 _
30
31
32 _
33
34
35
Comments:I !
Client:Location:
Coll. Date:WL/T
<"- 27 -o <rCr
/• £**,£ ^L**s>f
Preiim. Sorter:
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETJob #/Task/Labor Code:
Sample ID:
ID Time Budget: £a?Presort ID Time:
Split ID Time:Chiro. ID Time:
Split Sorter: ?.oSplit:Subsample: Erflbne Q Abundant Taxa:
DRBPIII: 0100 D 200 0 300 (Spec. Subsamp.). ' . ' . ' . -.(__ of grids)
O Other
Date^Date:_Date"
-QC .Info: D Sort QC.and/or D .ID QCID QCTime: Date:" 'By:
QC TaxonomicCheck Order Taxon
Total Presort Split•Number = Number + Number Notes
i _
2_
3
4
5
6
7
8
e10
1V_
12_
13
14_
15_
16
oTf 3
AK
/
( Lr)
ko^ so
20 _
21 _
22_
23 _
24 _
25_
26 _
27 _
28 .
29 _
30
31
32 _
33
34
35
Comments: g£*2 ' M;l ^.L &fc^ S2j2*t~~~Page: f of /
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: \j ,',
Location: ^4rColl. Date:
. f n rv\ - Job #/Task/Labor
C \L
Prelim. Sorter: _ Split Sorter:Subsample: KjNone D Abundant Taxa:
•DRBP
Q Other
Split:01 00 D 200 D 300 (Spec. Subsamp.)
- . (__ _ of grids)
ID Time Budget:Presort ID Time: /. p
Split ID Time:Chiro. ID Time:
'QC.Info: D Sort QC.and/or D ID QCl D Q C T i m e : D a t e f B y :
QC :
CheckTaxonomic
Order
TotalTaxon Number =
PresortNumber j
Split
i- Number Notes One. Bait QC Number)
<^fAC 'lYfTfcL/ZJL ^U.v^oo^u^ 4 - ""
rr
E
7
B_
9
10
11
13 _
14
20_
21-22_
23 _
24 .25 _
26 _
27 _
2B
29 _
30
31 _
32 _
33
34
35
Comments: *a<?LS ( 01* rr!>£i.
Page: / of
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET
Client: \/ta(V •' &!en»!*b~ /k?*~r _ Job ft/Task/Labor Code: lotoi,cnj I <*>'Location: V , & ' (' w Sample ID:
Coll. Date: - r^pW ~ " ID Time Budget: <2.0Prelim. Sorter: Jffjfe Split Sorter: — Presort ID Time: Date: By:_
Subsample: Bfxlone D Abundant Taxa: Split: Split ID Time: Date; By:'DRBPIII: Q100 D20D Q30D (Spec.subsamp.) Chiro. ID Time: Date: By:'
'. '.. ' . ' -..( of grids) QG Info: D Sort QC. and/or DID QCD Other:__ ID QC Time: Date:' By:
QC .•• • • TaxonomicCheck Order Taxon
Total Presort SplitNumber = Number + Number Notes flnc.BoitacNumb.r)
1 T^Mfi /s n ,,r (?,fc*A 3 ^ 3— - . . /
n . .
3 f)(^4. df(rr,t^/« • ?!? frZ /)d /t,~.dr/ /&£s
4 " Vc
7
B
B
17
18
19
20 _
21 -22_
23 _
24
25_
26 _
27 _
2B_
2B_
3D_
31 _
32 _
33
34
35
L..LLM
Comments:
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: ]/i*s<n~ -&/*>»fa fr* Job #/Task/Labor Code: -20f0\, &> / &
location: Z"A«/z <^/, -' /ynut/rr /L^S> Sample JD:Coll. Date: f
Prelim. Sorter: Split Sorter:Split:Subsampie: D None Q Abundant Taxa:
DRBPIII: Q100 D20D D300 (Spec.Subsamp.). - . • • - • •-.( -o f grids)
D Other:
ID Time Budget:Presort ID Time:
Split ID Time:Chiro. ID Time:
Date:_Date:Date7"
By:.By:..By:.
:QG.Info: D Sort QC.and/orO JD QCID QC Time: Date:' ' By:
QC TaxonomicCheck Order Taxon
Total Presort SplitNumber = Number 4 Number Notes (Inc. Sort QCNumbv)
2
3
4
5
6
7
11
16
21
23 _
24 _
25_
26 _
27-2B_
2B_
3D
31 _
32 .
33.
34
35
7 •' 7
Comments:
INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET
-Locaflon:
Coll. Date:Oi o t V?
Prelim. Sorter: Split Sorter: _Subsamplef EGNone D Abundant Taxa: Split:
D RBP III: D 100 D 200 D 300 (Spec. Subsamp.)
-.(__ -of grids)X9 Other . blOrAgLcK /"jowtje w i-Q
Job #/Task/LaborSample ID:
ID Time Budget:Presort ID Time: /, Q
Split ID Time:Chiro. ID Time:
*Date:DaterDate:
QC. TaxonomicCheck Order
By:..By:.
QC.Info: D Sort QC,and/or D JD QCID QC Time: _ __ Date:' 'By:
TaxonTotal Presort Split
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1 1 - • • • • • • . • • • • • • • . '
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/ - ) '
1 5 . . . . . .
16 /
1B.19_
21 _
22_
23_
24
25_
26
27 .28.29.30
31.
32 _
33
34
35
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Ol J
Comments: n>
Page: / of /._
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