NORMANDEAU ASSOCIATES, INC. EPA Region 5 Records Ctr ...

NORMANDEAU ASSOCIATES, INC. EPA Region 5 Records Ctr. ^ ' *3450 Schuylki/l RoadSpring City, PA 19475-1124(610)948-4700 237584(610)948-4 752 (Fax)www.normandeau.com

23 July 2004

J U L 2 6 ^ ' >Mr. Russ CepkoCBS CorporationBloomington ProjectGateway Center11 Stanwix Street, Room 628Pittsburgh, Pennsylvania 15222-1384

SUBJECT: Fish and Crayfish Tissue Sampling for Aroclor and Lipid Analysis at StoutsCreek near Bennett's DumpNormandeau Project No. 20103.000

Dear Russ:

Normandeau Associates has completed fish and crayfish sampling from three locations in Stout'sCreek (Monroe County) near Bloomington. Indiana. Sampling was conducted to obtain fish tissuesamples from three locations near Bennett's Dump, and crayfish samples from one location. Thesamples were shipped via Federal Express to En Chem Labs in Green Bay, Wisconson and AXYSAnalytical Services in Sidney, British Columbia for PCB (Aroclor or Congener as applicable) andlipids analysis. Copies of all data sheets and the Chain-of-Custody forms are enclosed.

Fish were collected using a pram electrofishing unit consisting of hand-held anodes and a pram-mounted cathode (McLaren/Hart 1996). Direct current was supplied from a gas-po\veredgenerator set at 230 volts with an output of approximately 5 amperes, depending upon theconductivity of the water at each sample location. Sample locations were as follows:

Station Location

BD-i Stout's Creek, Monroe Co., near West Hunter Road, downstream from Bennett'sDump (upstream section)

BD-2 Stout's Creek, Monroe Co., near Acuff Road (middle section)

BD-3 Stout's Creek, Monroe Co., near its confluence with Beanblossom Creek (MapieGrove Road)

Targeted fish species were creek chub (Semotilus atromaculctus), an omnivore: Green sunfish(Lepomis cyanellus) or longear sunfish (Lepomis megaloiis), pelagic water column feeders; andwhite sucker (Catostomus commersoni), a bottom feeder The field effort was designed to collectfish and crayfish as follows (Viacom, 2004):

Bedford, NH, Corporate

Norfolk. CT Hanover. MA Haverstraw. NY Aiken. SCLewes. DE Hampton. NH Drumore. PA Stevenson, WAYarmouth. ME Westmoreland. NH Spring City, PA

An Employee-owned Company r1^

NORMANDEAU ASSOCIATES, INC.

Station BD-1 6 green sunfish between 20 and 40 grams for 6 whole body Aroclor analyses3 green sunfish between 20 and 40 grams for 1 whole body Congener analysis3 green sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs greater than 20 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers greater than 30 grams for 6 whole body Aroclor analyses3 white suckers greater than 30 grams for 1 whole body Congener analysis

enough crayfish greater than 5 grams each to produce 3 composite samples forwhole body Aroclor analysis

Station BD-2 6 green sunfish greater than 20 grams for 6 whole body Aroclor analyses3 green sunfish greater then 20 grams for 1 whole body Congener analysis3 green sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs between 25 and 55 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers greater than 30 grams for 6 whole body Aroclor analyses3 white suckers greater than 30 grams for 1 whole body Congener analysis

Station BD-3 6 longear sunfish between 20 and 50 grams for 6 whole body Aroclor analyses3 longear sunfish between 20 and 50 grams for 1 whole body Congener analysis3 longear sunfish (largest available) fillets for 3 Aroclor analyses6 creek chubs greater than 20 grams for 6 whole body Aroclor analyses3 creek chubs greater than 20 grams for 1 whole body Congener analysis6 white suckers between 30 and 50 grams for 6 whole body Aroclor analyses3 white suckers between 30 and 50 grams for 1 whole body Congener analysis

Stream sections of several hundred meters were shocked at each station until sufficient fish werecollected to meet the study specifications.

Captured fish and crayfish were placed in aerated buckets for 24 hours, prior to sampleproduction, to purge their gut contents; or their gastrointestinal tracts were removed. Wirescreening was attached to the inside of the buckets to allow fecal material to pass through andprevent reingestion.

The fish samples analyzed for PCB Aroclors consisted of single individuals. Fish samplesanalyzed for Congeners were composites of 3 individuals each. All fish and crayfish samplescontained at least 20 grams of tissue. All replicate analyses were conducted at the respectivelaboratories. Split samples were submitted in the field as requested to Ms. Regina Bergner(Tetra Tech), who provided oversight on behalf of US EPA, and analyzed by US EPA'scontract laboratory.

Each sample and an equipment blank, were given a two-digit project identification code (BD forBennett's Dump) and a four-digit sequence number. Sample numbers were not repeated.


The length/weight regressions are augmented with data derived from scale samples taken fromtarget species. Scales were removed from two fish per species at each station: the longest one anda second of intermediate length. The age of each fish was determined by counting the number ofannuli, and the length of each fish following each year's growth (Murphy and Willis 1996),calculated from measurements of scale radius according to the formula:

Ln = (Lc - a / Sr * Sn) + a where:

Ln = the length at annulus (the end of each year's growth)Lc = the length at captureSr = the scale radiusSn = the radius to annulus na = the y-intercept of a regression of body length scale radii for 18 fish of the species

captured from Stouts Creek

Lastly, the stomach contents of 10 to 15 fish for each target species, at each station, were purgedand the invertebrates contained within identified and counted. Samples were produced either byforcing water through the stomach of each fish with a syringe to collect back-washed food itemsin a pan or by removal of the stomach contents by dissection. Food items were then compositedby species and sample station, preserved in alcohol, and transported to our lab for analysis.Invertebrates were identified to either genus of the lowest taxonomic level possible given their ageand condition.

Results

A total of 25 species (Table 1) was collected from Stouts Creek (all stations combined), indicatinga moderately rich fish community. Two additional species (tadpole madtom and shortheadredhorse), collected in 1998 but not in 2004, increases the total to 27. The dominant species atone or more stations were minnows: creek chub, central stoneroller, or bluntnose minnow.

At Station BD-1, 733 fish but only 11 species were collected. A community density of 31,912fish per hectare (ha) and 228.8 kilograms per hectare (Table 2), was consistent with what wouldbe expected from a stream flowing through karst topography, where productivity is typically high.Most common were creek chubs (19,156/ha, 156.3 kg/ha) that represented 60.0 percent of thenumerical total. Green sunfish 7,906/ha, 52.4 kg/ha) represented an additional 21.8 percent ofthe total. All other species comprised less than 10 percent.

At BD-1. length-frequency histograms were assembled for green sunfish and creek chubs (Figure1). An insufficient number of white suckers (the third target species) was collected to produce ameaningful histogram. Results showed that the majority of fish within these two species were toosmall to be considered harvestable by most anglers. Only 12 of 104 (11.5 pet.) green sunfishmeasured exceeded 100 millimeters (approx. 4 inches) and all were less than 150 millimeters(approx. 6 inches). Only 4 of 100 (4.0 pet.) creek chubs exceeded 150 millimeters. Using theselengths as a basis, the density of green sunfish and creek chubs most likely to be harvested at BD-1 was low. Application of the percentage of fish longer than 100 or 150 millimeters to therespective population estimates produced values of 22 harvestable green sunfish and 19harvestable creek chubs per 100-meters of stream channel.

NOHMANDEAU ASSOCIATES, INC.

At Station BD-2 channel width and surface area increased; hence, the fish community was not asconfined as was it was upstream. Increased stream gradient produced swifter current velocities;and the substrate composition changed. The streambed consisted mostly of cobble/gravel, wheresand/silt was predominant at BD-1. A species-poor community was also observed at BD-2 where10 species, and 902 fish, were collected. Community density decreased to 18,976 fish and 91.7kilograms per hectare. The dominance hierarchy changed relative to that observed upstream ascentral stoneroller became the most abundant species. Stonerollers comprised 37.5 percent( 7 , I l l / h a , 24.0 kg/ha) of the cumulative total. Co-dominant were creek chubs, a larger-sizedspecies, that represented 35.3 percent (6,768/ha, 48.7 kg/ha) of the total. Third in abundancewas blacknose dace, 13.2 percent (2,537/ha, 8.2 kg/ha).

At BD-2, length-frequency histograms were produced for green sunfish and creek chubs (Figure2). No green sunfish measured exceeded 100 millimeters. Only 2 of 100 (2.0 pet.) creek chubsexceeded 150 millimeters. Thus, the density of green sunfish and creek chubs most likely to beharvested at BD-2 was again low, 0 and 7 fish per 100 meters.

At Station BD-3 current velocity decreased and the streambed changed back to one consistingmostly of sand/silt. Community richness, near the confluence of Stouts Creek with BeanblossomCreek, was nearly twice that observed upstream. Twenty-three species were collected.However, community density further decreased to 12,089 fish and 57.1 kilograms per hectare.The dominance hierarchy changed again, as bluntnose minnow became the most abundantspecies. Bluntnose minnows represented 48.5 percent (5,872/ha, 11.7 kg/ha) of the total,followed by creek chub at 18.7 percent (2,300/ha, 8.4 kg/ha), and central stoneroller at 9.5percent (1,152/ha, 3.7 kg/ha).

Length-frequency histograms were assembled for all four target species (Figure 3). Eleven of 25(44.0 pet) green sunfish and 9 of 38 (23.7 pet.) longear sunfish measured exceeded 100millimeters. Because the population estimates for these panfish were relatively low, calculationsof the number of each species longer than 100 millimeters remained low also. The number oflarger green sunfish per 100 meters of stream channel was 11 and the number of larger longearsunfish was 9. Only 1 of 104 (1.0 pet.) creek chubs and no white suckers exceeded 150millimeters. Estimates for the number of larger fish for these species were 1 and 0 per 100meters, respectively.

Note that larger green sunfish (BD-2) and white suckers (BD-1 and 2) were collected for tissueanalysis but very few or none were found in the 100-meter stream sections established for thepopulation estimates.

Length/Weight relationships from Stouts Creek (all stations for 1998 and 2004 combined) areshown for each target species as Figures 4 through 7. The regression equations shown on thefigures can be used to calculate the weight (y variable) for any fish within the range of lengths (xvariable) encompassed by the fish submitted for analysis. For each species applicable ranges are:

Green Sunfish 90 - 155 mm Creek Chub 110-240 mmLongear sunfish 95 - 155 mm White Sucker 130-290 mm


A total of 72 fish and 3 crayfish samples were collected from the three stations and submitted foranalysis. A Chain-of-Custody form was included noting: the identity of the fish used for eachsample, the sample type (whole body or fillet), whether the sample consisted of a single individualor was a composite, and the sample weight.

Samples were submitted in accordance with the QAPjP Plan pertaining to this project with oneexception. Three white suckers used to produce the composite sample for Congener analysis atStation BD-3 exceeded the 30 to 50 gram size range. They were between 79 and 92 grams.

Ecological Study

To obtain information regarding species composition, population density, size distribution oftarget species, length/weight relationship of target species, and the food items selected by targetspecies, quantitative samples were collected at each station.

Methods

The electrofisher was used to produce species composition and population estimates fromrepresentative 100-meter stream sections that encompassed at least one run-riffle-pool habitatsequence at each station. A tape was used to delineate this length, and a series of 10 widthmeasurements were averaged to calculate the respective surface areas sampled (in hectares: 1hectare = 2.47 acres). Depletion data from three consecutive capture efforts at each station wereregressed to produce population estimates for each species and for all species combined (VanDeventer and Plaits 1983). Source data included the total length and weight of each fish and thecumulative weight of all fish collected for each species. Results were calculated as the number offish per 100-meters of stream channel, and the number of fish and biomass per hectare. Anyadditional species collected from the greater shocking effort employed to obtain target fish fortissue analysis, but not present in the 100-meter stream sections used for the depletion estimates,were noted.

Length-frequency distribution, in 5-millimeter increments (by station) for the four target speciesare shown as a series of histograms. Each histogram displays the data for the first 100 fish(approx.) measured from each species. If fewer than 100 individuals were captured fromparticular species, all were included. Note that lengths were taken for non-target species andlength-frequency distributions can be derived for those as well.

The length/weight relationship for each target species (all stations combined) is shown as a seriesof regression plots. Data used to produce the plots were taken from fish submitted for tissueanalysis. To provide enough data to produce meaningful plots, lengths and weights recordedfrom a nearly identical collection effort at the same three stations in 1998 were pooled with thesedata from 2004 Plots are given for both natural log (In: base e) transformed and untransformeddata.


As an example: the formula: y = 3.375x - 12.418, from Figure 5a for the two longear sunfishlengths shown on Table 3 produces the following weights:

Length In length In weight Calc.wt. Meas. wt.

105mm 4.65396 3.89115 27 grams 28 grams155mm 5.04343 4.60356 99 grams 87 grams

The calculated weight of 27 grams, for the 105-millimeter longear, compares well with theweight measured in the field of 28 grams (see Table 3). For the 155-millimeter fish, thecalculated weight of 99 grams differs from the field measurement by 12 grams, showing thatweights of individual fish of equal length can vary. Regression equations average this intra-specific variance. The most accurate results will be obtained by using log (natural log: base e)transformed length data. Correlation coefficients (R2) for the regressions shown on figures 4a,5a, 6a, and 7a range between 0.84 and 0.96 so the formulas are accurate.

Table 3 provides length calculations for target species following each year of growth, referred toas mean length at annulus. That is, the analysis correlates age and length. These results aresummarized as follows:

Mean Length at Annulus (mm)Species Year 1 Year 2 Year 3 Year 4 Year 5

Creek Chub 54 98 129 163 172White Sucker 111 148 209 268Green Sunfish 74 89 104 122 133Longear sunfish 60 79 106 121 152

The results of the stomach contents analysis are given in Table 4. The analysis was interesting inthat it showed a considerable degree of resource partitioning between the different species. Creekchubs, the omnivore, were feeding mostly on aquatic earthworms (Lumbriculidae) and crane flieswithin the genus Tipula; relatively large-sized invertebrates found in depositional habitats.Stomach contents from the bottom feeding white suckers contained smaller sized items, mostlymidges (several genera in the family Chironomidae) and a crustacean in the genus Lirceus, bothfound in depositional areas. Resource partitioning was also indicated from the contents of the twopelagic feeding sunfish species. The diet of green sunfish consisted mostly of Lirceus andLumbriculidae, while chironomids were the predominant food item found in the longear sunfishstomachs.

The diet of all target species was comprised mostly of forms adapted to depositional environments(Merritt and Cummins 1996) where fine sediments and paniculate organic matter accumulate, andthe potential for sorption of PCB molecules is relatively high.

Sincerely, L

George M. Christian(Project Biologist)


References:

Merritt R. W. and K. W. Cummins, editors. 1996. An introduction to the aquatic insects ofNorth America, 3rd edition. Kendall/Hunt Publishing Company, Dubuque, Iowa.

McLaren/Hart, 1996. Field procedure no. 23: Protocol for evaluating fish populations andsampling fish tissue. ChemRisk (a Division of McLaren/Hart), Portland, Maine, 4October 1996.

Murphy, B. R., and D. W. Willis, editors. 1996. Fisheries techniques, 2nd edition. AmericanFisheries Society, Bethesda, Maryland.

Normandeau Associates, Inc. 1998. Fish and crayfish tissue sampling for aroclor and lipidAnalysis near Neal's Landfill and Bennett"t Dump (letter report). Normandeau (ProjectNumber 17750.000), Spring City, Pennsylvania, 6 May 1998.

Van Deventer, J. S., and W. S. Platts. 1983. Sampling and estimating fish populations fromstreams. Transactions of the North American Wildlife and Natural Resources Conference48: 349-354.

Viacom, Inc. 2004. QAPjP, Volume XXXIX: Bennett's Dump, Stout's Creek fish, water, andsediments sampling and analysis plan. Viacom. Pittsburgh, Pennsylvania, Spring 2004.

Table 1. Fish Collected from Stouts Creek, Monroe County, Indiana near Bennetts Dump.

Sample Date:Gear:

Scientific Name

AmiidaeAmia calva

CyprinidaeCampostoma anomalumCyprinus carpioLuxilus chtysocephalusLythrurus umbratilisNotropis buccatusNotropis hudsoniusPimephales notatusRhinichthys atratulusSemotilus atromaculatus

CatostomidaeCatostomus commersoniErimyzon oblongusHypentelium nigricans

IctaluridaeAmeiurus natalis

PoeciliidaeGambusia holbrooki

CentrarchidaeAmbloplites rupestrisLepomis cyanellusLepomis macrochirusLepomis megalotisLepomis hybridMicropterus salmoides

PercidaeEtheostoma caeruleumEtheostoma flabellareEtheostoma nigrumPercina carbonaria

25-27 May 2004Electrofisher (barge unit: three pass depletion estimate)

BD-1Common Name Number Percent

bowfin

central stoneroller 40 5.5%common carpstriped shiner Predfin shiner

silverjaw minnowspottail shiner P

bluntnose minnow 7 1.0%blacknose dace 55 7.5%

creek chub 440 60.0%

white sucker 3 0.4%creekchub sucker

Northern hog sucker

yellow bullhead

Eastern mosquito fish

rock bassgreen sunfish 160 21.8%

bluegill sunfish Plongear sunfishhybrid sunfish

largemouth bass

rainbow darter 10 1.4%fantail darter 18 2.5%johnny darter

logperch

Total Fish for Three Passes Combined 733 1 00.0%Duration for Pass Number 1 (minutes) 55 min.Section Length (meters)Area Sampled (hectares)

Total Species/Hybrids (stationCatch Rate for Pass Number 1

100m0.0244 h

total*) 11(fish/hr.) 518

BD-2Number Percent

338 37.5%

18 2.0%1 19 13.2%318 35.3%

3 0.3%

P

35 3.9%

P

14 1.6%57 6.3%

902 100.0%44 min.100m

0.0495 h

10850

BD-3Number Percent

P

54 9.5%PP16 2.8%3 0.5%6 1.1%

275 48.5%1 0.2%

106 18.7%

14 2.5%5 0.9%

P

4 0.7%

1 0.2%25 4.4%1 0.2%

38 6.7%4 0.7%

1 0.2%2 0.4%8 1.4%3 0.5%

567 100.0%38 min.100m

0.0495 h

23536

Water Quality:

Water Temp. (C)Dissolved Oxygen (mg/1)Conductivity (micro-siemans/cm @ KT)Specific Conductance (micro-siemans/cm @ K25)

21.08.4485525

20.56.4560613

Note:

1) The total number of species captured at each station was derived by adding any additional species from the shockingeffort expended to collect fish for tissue analysis to those from the population estimate. These are denotedwith a P (present) but are not quantified. Two additional species (tadpole madtom and shorthead redhorse) werecollected from Stouts Creek in 1998 but not in 2004.

Table 2. Population estimates calculated from fish data collected from Stouts Creek during 25 - 27 May 2004.Estimates are presented for each species and for all species combined.

Area (Hectares):

Est.

779

Species:

creek chub 467central stoneroller 42blacknose dace 84bluntnose minnow 7fantail darter 79rainbow darter 13white sucker 3green sunfish 193longear sunfishmosquitofishhybrid sunfishjohnny dartercreek chubsuckersilverjaw minnowlogperchspottail shinerredfin shinerbluegillrock bass

Sum (QA/QC)

0.0244

BD1no./ha

Pooled

31912

By Species

19156174134262853233518132

7906

36398

kg/ha

228.8

156.39.79.23.22.70.75.652.4

239.8

0.0495

BD2Est no./ha

Pooled

939 18976

By Species

335 6768352 7111126 253720 40182 165818 3594 7439 783

19690

kg/ha

91.7

48.724.08.20.62.20.54.65.2

94.1

Est.

598

114571

291211526404485331121101

0.0495

BD3no./ha

Pooled

12089

By Species

2300115220

587247203035258088184162926154

21842220220

12443

kg/ha

57.1

8.43.70.011.70.11.00.511.114.60.10.40.20.30.20.54.71.21.60.1

60.5

Figure 1.Length Frequency Distribution of Green Sunfish at North

Hunter Road (Station BD #1)I Green Sunfish

Length (mm)

Length Frequency Distribution of Creek Chubs at NorthHunter Road (Station BD #1)

w -|2 -Ta•n 10 -

"O Q

£o 6i_•Q A. -Ez 2

0 _

10

• Creek Chub

12

6 6

.1ill

! S 5! ° g> r— oo

Total N = 100

6

4 4 •

n n n 1,11Illlllll UNI | 0 0 | 0 | 0 0 0 0 0 0 0 0 0 | |

3 S S S S S 5 S

Length (mm)

Figure 2.Length Frequency Distribution of Green Sunfish at Acuff

Road (Station BD #2)

D Green Sunfish

Length (mm)

Length Frequency Distribution of Creek Chubs at Acuff Road(Station BD #2)

on

• Cree

181ft

(0"a 16•o 14 _

« 17 -

- 100 8

1 2-i-1 2T

o-t

tChub

Total N = 100

13

8 8

Iu 6 *\ • 4 4 ^t riIII. III!

^ ^ C S ^ " ® ^ ' " ® ^ " * ' * 5 * " ® ^ 'm < D ( y h - r * - c o « o a j a > o O T -

' S S S ^ K S ^ ^ ^ g S S

4

I 21 • 1 1 1 10 I . 0 , 0 0 0 0 0

• • • • • • •? s a s s ? ? s s s s s s si / > o i n ^ > i 6 d i i O ( 6 u S ( 6 i f > o i / k ( ^T - c M c v j c o o 5 ^ - ^ r i n i n < 5 i D ^ r * . o B

Length (mm)

Figure 3.Length Frequency Distribution of Green Sunfish at Maple

Grove Road (Station BD #3)

D Green Sunfish

Total N = 25

Length (mm)

Length Frequency Distribution of Creek Chubs at MapleGrove Road (Station BD #3)

-inMra OK3 £.3

'"O

>t- 15 -o 13

X 10 -4

E3 cz ° H

0 _

• Cree

2°

17

3 3

.11

16 17

kChub

TV-it oi M — -i ne1 Otai N — 1 Uo

1 10

il0 1 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1

• • • • •t ' ^ ' f f t ' ^ ' O t ^ o t ' ^ ' t n ^ f O t ' ^ ' C f t ^ ' O> T - ^ - < N j c M O ( O ^ - ^ - t f > u > « ) 2 > h * h - .

! S ! 8 e R § S i ^ | ^ 6 . p g ^ ^ . A 6 . A 6 . A ^ . A 6 ^

Length (mm)

Figure 3 (cont).Length Frequency Distribution of White Sucker at Maple

Grove Road (Station BD #3)

7

1 632 5

1 4-

•5 3i_S o

Z 1

D White Sucker

Total N = 146

5

2

I 1

0 0 0 0

s s $ $ s $

0 0 0

S 8 S& £ 8Length (mm)

0 0 0 0I I i i

£ £ £ s i S

8stm

•o

= 4

I 2z

0

Length Frequency Distribution of Longear Sunfish at MapleGrove Road (Station BD #3)

D Longear Sunfish

6 6

I

4

2

7Total N = 38

2 2 2

1 1 1 1 1 1

£Length (mm)

4.5

2.5

Figure 4a.Green Sunfish Length/Weight Relationship (Log base e transformed)

from Stouts Creek

|y = 3.2039x-11.84R2 = 0.8403

4.5 4.6 4.7 4.8 4.9

In (length in millimeters)

Green Sunfish

• Linear (Green Sunfish)

5.1

100

90

80

70

w 60

I01= 50

I)

^ 40

30

20

10

Figure 4b.Green Sunfish length/Weight Relationship (Untransformed)

from Stouts Creek.

y = 1.0326x - 87.754

R2 = 0.7422Green Sunfish

•Linear (Green Sunfish)

90 100 110 120 130

length in millimeters

140 150

Figure 5a.Longear Sunfish Length/Weight Relationship (Log base e transformed)

from Stouts Creek.J -

4 C

4 -go>.££O)

3

2 C

Longear Sunfish

Linear (Longear Sunfish)

4.5 4.6 4.7 4.8 4.9 5 5.1


90

80

70

60

E 50O)

40

30

20

10

85

Figure 5b.Longear Sunfish Length/Weight Relationship (Untransformed)

from Stouts Creek.

95 105 115 125


135 145 155

Longear Sunfish

Linear (Longear Sunfish)

4.9

4.4

O)

,E£

3.9

3.4

2.9

4.7

Figure 6a.Creek Chub Length/Weight Relationship (Log base e transformed)

from Stouts Creek.

y = 2.8322x-10.456

R2 = 0.8931

4.8 4.9 5 5.1 5.2


5.3 5.4 5.5

Creek Chub

•Linear (Creek Chub)

200

180

160

140

M 120

5o>.S 100

s>95 80

60

40

20

100

Figure 6b.Creek Chub Length/Weight Relationship (Untransformed)

from Stouts Creek.

y=1.0678x-113.81

R2 = 0.8535Creek Chub

•Linear (Creek Chub)

120 140 160 180


200 220 240

6

5.5

in

5en

4.5

3.5

Figure 7a.White Sucker Length/Weight Relationship (Log base e transformed)

from Stouts Creek.

y = 2.9835x-11.322R2 = 0.9555 White Sucker

Linear (White Sucker)

4.8 4.9 5 5.1 5.2 5.3 5.4 5.5


5.6 5.7

260

10

Figure 7b.White Sucker Length/Weight Relationship (Untransformed)

from Stouts Creek.

130 150 190 210 230


White Sucker

Linear (White Sucker)

250 270 290

Table 3. Age and Growth Relationships Calculated from Scale Samples Collected fromStouts Creek, Monroe County, Indiana on 25 - 27 May 2004.

Station

BD-1BD-1BD-2BD-2BD-3BD-3

BD-1BD-1BD-2BD-2BD-3BD-3

BD-1BD-1BD-2BD-2

BD-3BD-3

underlined

Species

creek chubcreek chubcreek chubcreek chubcreek chubcreek chub

white suckerwhite suckerwhite suckerwhite suckerwhite suckerwhite sucker

green sunfishgreen sunfishgreen sunfishgreen sunfish

longear sunfishlongear sunfish

Length

158124130235135175

145196163282142267

101138113153

105155

Weight Age

5723251713175

mean length

339646o32203

mean length

21482180

mean length

2887

mean length

rvranIVrvV

Yearclass

200020012002200020001999

at annulus

IIIInIVi

IV

200320012002200020032000

at annulus

rvVnirv

2000199920012000

at annulus

rvV

20001999

at annulus

Length (mm) atI

424190763936

54

133921268812899

111

68677587

74

5863

60

II

1091011301096874

98

122163153

153

148

798890101

89

7287

79

III

132124

166109116

129

166

234

225

209

91104108114

104

93120

106

annulusIV

158

212135146

163

268

267

268

98121

146

122

103138

121

V

172

172

133

133

152

152

= annulus judged to be at scale margin

Table 4. Stomach Contents of Fish Collected from Stouts Creek, Monroe County, Indiana near Bennetts Dump.

Sample Date:Gear:

Taxon:

Creek Chub:

Terrestrials

ChironominiChironomusDugesiaGammaridaeLirceusLumbriculidaeOrconectesTabanusTanytarsiniTipula

White Sucker:

CeratopogonidaeChironominiChironomusCopepodaEphydridaeLirceusMicrotendipesOptioservusOstracodaPhaenopsectraProcladiusSphaeriidaeSphaeromiasTanypodinae

Tanytarsini

Sunfish:

Terrestrials

Fish Tissue

CheumatopsycheChironominiChironomusDubiraphiaErpobdellidaeGammaridaeLirceusLumbriculidaeMicrotendipesOrconectesPolvpedilumProcladiusTanypodinae

TanytarsiniTipula

25-27 May 2004Electrofisher

CommonName

1 7-yr cicadamillipede

midgemidge

flatwormsideswimmer

sowbugearthworm

crayfishhorse fly

midgecrane fly

biting midgemidgemidge

fish liceshore flysowbugmidge

riffle beetleseed shrimp

midgemidge

fingernail clambiting midge

midge

midge

1 7-yr cicadacaterpillar

minnow

caddisflymidgemidge

riffle beetleleech

sideswimmersowbug

earthwormmidge

crayfishmidgemidgemidge

midgecrane fly

Habitat

.-

lentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional1 enti c/loti c-depositionallentic/lotic-depositionallentic/lotic-depositional

lentic-erosional/depositionallotic-erosional/depositional

lotic/lentic-erosional/depositional

lentic/lotic-depositionallentic/lotic-depositional

lentic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional

lotic-eorsionallentic-depositionallentic-depositional

lentic/lotic-depositionallentic/lotic-depositional

lentic-depositionallotic/lentic-

erosional/depositionallotic-erosional/depositional

--

-

lotic-eorsionallentic/lotic-depositionallentic/lotic-depositional

lentic/lotic-ersionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositionallentic/lotic-depositional

lentic-depositionallentic/lotic-depositional

lotic/lentic-erosional/depositional

lotic-erosional/depositionallotic/lentic-

erosional/depositional

BDNumber

31

60

1

39

12

11811

1

1

3

1

1

1

2383

3

13

-1Percent

2.9%1.0%

57.7%

1.0%

37.5%

5.9%1 1 .8%

5.9%5.9%

47.1%5.9%5.9%

5.9%

5.9%

BD-ZNumber Percent

2 2.6%1 1.3%3 3.8%1 1.3%1 1.3%

65 83.3%3 3.8%

1 1.3%1 1 .3%

147 68.4%25 11 .6%5 2.3%

8 3.7%10 4.7%

3 1.4%10 4.7%3 1 .4%

3 1.4%

1 0.5%

Green

2.5%

0.8%

0.8%

0.8%

19.3%69.7%

2.5%

0.8%2.5%

7 16.7%33 78.6%

1 2.4%1 2.4%

BD-3Number Percent

1 14.3%

6 85.7%

2 22.2%4 44.4%

1 11.1%

2 22.2%

Longear

5 8.1%1 1.6%

1 1 .6%

3 4.8%12 19.4%17 27.4%1 1.6%

1 1 .6%1 1.6%2 3.2%7 1 1 .3%

6 9.7%1 1.6%4 6.5%

NORMANDEAUASSOCIATES

GENERAL FISHERIES INFORMATION SHEETf*^1i#»nf" / /• /? ' ' & ' *•

Job NumbeLocation (CLocation (SSample DatGear Type:Purpose of

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GENERAL FISHERIES INFORMATION SHEETClient: \f \Ater^ '. ^ /«« 'n /»J j^ j j PffjeCr

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NORMANDEAU ASSOCIATES

GENERAL FISHERIES INFORMATION SHEET

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NORMANDEAU ASSOCIA TES

GENERAL FISHERIES INFORMATION^ ^ t f A M ^ V 1 / t ft? / / ?^ÎICQU \tf<'fff^-l ' f-3 (WHfit fail

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NORMANDEA U ASSOCIA FES

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GENERAL FISHERIES INFORMATION SHEETClient >Job Number:Location (General):,Location (Specifier. &) - ?Sample Date: AS. M /tftyGear Type: '£,0 A A^Purpose of Sample /f# A*

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NORMANDEAU ASSOCIA TES

GENERAL FISHERIES INFORMATION SHEETClient: \/i«c«m: Ulwi/n/o^^ P'«u«fJob Number: 3oi°i- revLocation (GeneraTc 5|cu.f5 (V«t (6"JA/<*b DJ^P)Location (Specificc 8 & - ?> ( m* cu t, /< « "> Start Time:Sample Date: <S5,3|/ /*>»/ 2o°y End Time:Gear Type: 5m* n P'*^Purpose of Sample PC 6 Auat/sij

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NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: _

Address: //3450Schuylkill RoadSprincLCJl uEA 19475-1124

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*Matrix Codes:S - soil GW - ground waterSE - sediment ST - stormwaterSL - sludge PW - processed waterO - oil DW - dilution waterWW - waste water RW - receiving water XSW - surface water X - other (_ /fyy^,

Samples Were:1 . ,8rTfpp*bd or hand delivered 2^CTiifi5tijOr ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels andJCO£ record?

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COC Tape Was: 1. Present on outer packageYes No

3. Received broken / leakingYes No

2. Unbroken on outer packageYes No

Whito r~r~, DMr~

4. Received within holding timesYes No

5. Discrepancies between sample labels and COC record?Yes No

3. Present on sampleYes No

4. Unbroken on sampleYes No

NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY

3450 Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752

Client:

Address:

Phone:

Samplers:Project No.:

f/ > W/ M f'C '</ Client Contact:

Sample Return To Client [ ]

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COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample S r~\ ff ~*~ *\Yes No Yes No Yes No Yes No ( ff ' 1

NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: £ A.-.V Client Contact:

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COC Tape Was: 1. Present on outer package 2. Unbroken on outer package 3. Present on sample 4. Unbroken on sample / -*y / —7 AYes No Yes No Yes No Yes No 1 /) t )

NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: i t f f rv>^

3450 Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752

Address:

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Project No.:

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Samples Were:1. ^higJB^d or hand delivered 2. phFjlgja or ambient 3. Received broken / leaking 4. Received within holding times 5. Discrepancies between sample labels and COC record?

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NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY

Ad3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752

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NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client: J)S i

3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752

Address:

Phone:

Samplers:

Project No.:

Client Contact:

Sample Return To Client [ ]

Disposal Disposal by Lab [ ]

•

1)•

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*Matrix Codes:S - soil GW - ground waterSE - sediment ST - stormwaterSL - sludge PW - processed waterO - oil DW - dilution waterWW - waste water RW - receiving waterSW - surface water X - other

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Yes No Yes No Yes No Yes No f £,£ "7 )

NORMANDEAU ASSOCIATES, INC.RMC ENVIRONMENTAL SERVICES DIVISIONAQUATIC TOXICOLOGY LABORATORY Client:

3450Schuylkill RoadSpring City, PA 19475-1124(610)948-4700 • Fax: (610)948-4752

Address:

Phone:

Samplers:

Project No.:

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Sample

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FISH SAMPLING -KEELD DATA SHEET (FRONT)page / of 2

STREAM NAME <?r LOCATION A///**'STATION * / RB'rRMILx STREAM CLASS

LA LONG RIVER BASIN

STORET # AGENCY

2EIESURVEV

GEAR INVESTIGATORS—iKiASONFOlJ SURVEYFORM COMPETED BY DATE

TIME

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GENERALCOMMENTS

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Block nets used? D YEE QHSfT

Sampling Ituration Start timf o<1ff End time /o^>

Stream width (in meters) Ma:: Mean "2.i¥/*t

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FISE SAMPLING -KEELD DATA SHEET (ERONTjpage of

STREAM NAME

STATION f 6 b * / RJArERMILr

LAT LONG

STORE! #

GEAR

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RIVER BASIN

AGENCY

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DATE !&bM REASON FOE. SURVEYTIME AW PM

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Block nets used? DYES DNO

Sampling Duration Start iim: End time

D other

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OPTIONAL: LENGTH (mm)AVElGHT (gj(25 SPECIMEN MAX SUBSAMPLEj

kavid Bioassessmeni Protocols For use in Stream; ana Wadeabic River: Pcnpnyior, benihic

FISH SAMPLING -FIELD DATA SHEET (FRONT)page / of

STREAM NAME

STATION* B t > * | RTVSRMILE

LAT LONG

STORET *

GEAR

FORM COMPLETED BY

| LOCATION

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AGENCY

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OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX STJBSAMPLE)

For Use in Sireams and Wadeabic Rivjr.1:. Pennhvior. Benth

FISH SAMPLING-FIELD DATA SHEET (FRONT)page / of /

STREAM NAME j LOCATION

STATION * tfD * 1 PJVSRMILE

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Cl") f2 î^ if' $TJ

( } % . / f

v9' ^/^/f^ ~ /«?,>,

OPTIONAL: LENGTH (mm)AVEIGHT(25 SPECIMEN MAX SUBSAMPLE)

Rapid Bioassexsmeni Protocols For Use m Sireams and Wadeabic River:. Pcnphywr,, henimc

FISH SAMPLING-MELD DATA SHEET (FRONT)pa?e £ of

STREAM NAME SVtu/r £>,

STATION * &D ' 1 RTVERMILE_

LAT LONG

STORET #

GEAR

FORM COMPLETED BY

LOCATION

STREAM CLASS

RIVER BASIN

AGENCY £/r F>>/

INVESTIGATORS

DATE S'-lA'0/ REASON FOR SURVEYTIME AM PM /) <? r/-

/'ci'7 £1 T' /

SAMPLECOLLECTION

« - > » '

HABITAT TYPES

GENERALCOMMENTS

How were tht fish capture

Block nets used? D YE

Sampling Duration Start

Stream width fin meters)

d? Dbaclcpack D tore bares Q other

£ DNO

dm: End rime Duration </</*'/<-'

Ma;' Mean

Indicate the percentage of each habitat type presentD Riffle: % D Pool.': % D ruins % D Snaps %D Submerged Macropnvtss % D Other 1 ) %

SPECIES TOTAL(COUNT)

OPTIONAL: LENGTH (mm)AVElGHT (g)(25 SPECIMEN MAX SUBSAMPLE)

37

131

Ji.

0

5V

Rapid Biocssessmem prowcois Fo:- Use in Sireams and Wadeabh River:. Pciiphyior,, beminc

FISH SAMPLING -PTELD DATA SHEET (FRONT)

STREAM NAME S-Leub Cfrefc.

STATION* 8D-3. RJVERMELE

LAT LONG

STORET*

GEAR

FORM COMPLETED BY

| LOCATION

STREAM CLASS

RIVER BASIN

AGENCY

INVESTIGATORS

DATE £~-S&c^ SEASON ran SURVEYTIME <&' AM PM /) J?_// c$ cy

SAMPLECOLLECTION

/? uM * ^

HABITAT TYPES

GENERALCOMMENTS

How were the fish captured?

Block nee used? D YES

Sampling Duration Start cm:

D back pack Diotebarpt D other

Q NO

/??! Endnmt /fir6 Duranon £'§*, ^

Srream width (in meters) Ma:: Meaii

Indicate the percentage of eachD Rifiiec % D Pool?D Submerges Macrophytss

habitat type present% D Rims % D Snaes • %

% D Other ( ) %

*

OPTIONAL: LENGTH (mm)AVEIGET (g)5 SPECIMEN MAX SUBSAMPLEj

FISH SAMPLING-FIELD DATA SHEET (FRONT)I of /

STREAM NAME SôuJrS Q fQL\i

STATIONS' BD-3- RTVERMILE

LAT LONG

STORET*

GEAR

FORM COMPLETED BY

! LOCATION

STREAM CLASS

RIVER. BASIN

AGENCY

INVESTIGATORS

DATE f •£?-<••<•? REASON FOR SURVEY-

TIME ^1t>' AM PM

SAMPLECOLLECTION

(?^*3

HABITAT TYPES

GENERALCOMMENTS


Block nets used? D YES

Sampling Duration Start nm:

D back pack D tott bargt D other

DNO

(46<l End time A? ? 7 Duration "21? ^

Stream width (in meters') MM: Mean

Indicate the percentage of eachD Riffles % D PoolsD Submerged Macrophvtes

habitat type present% QJttms % DSTiatK • %

% D Other ( 1 %


i' I'rnmm/c rnr Tivr in Sirp.nm': nn/! IrVnripnnii ' Rivr- Pfnn'mi

FISH SAMPLING -FIELD DATA SHEET (FRONT)of

STREAM NAME <T£t>/r C^JC

STATION * &D - 3 RJVERMILE

LAT LONG

STORET #

GEAR

FORM COMPLETED BY ^c:

LOCATION Jûo^ A*u.>

STREAM CLASS $c.j/, £r*u?

RIVER BASIN /&,,, yfâjt*.

AGENCT ^/^ £?rf

INVESTIGATORS S&C . ^). //IT. £**DATE ^-ô/ REASON Fonsufe-TIME - (^ PM /? &>J_ /T,1 X "fpJ. <L.S'- / / I-3S~C*J ôi</)'' • i

SAMPLECOLLECTION

/,„*/

HABITAT TYPES

GENERALCOMMENTS

How were the fish capture

Block nets used? D YI

Sampling Duration Start

Stream width (in meters)

d? D bade pad; BfoiebarEt D other

, BrfT ^^

nm: Ô."Jo End tims //-'» ^ -Durntipn- -? « /*/»*

Ma:: ^ Mear, ^ f?^ Kf ^^^'^(/Fe

Indicate thepercentage of each habitat typt presentCrfdfflK -i % Blôlr ^ % B-RUTIE £ % D Snaus • %D Submerged MacrDpbvteE % D Other ( 1 %

»,<$<* .' m ,3 r* n fg tf to) n A/ - foou.ft1) /c ^) /y #) ^ , » ^y//.^ /& to /a ^^> f7 >/*» « y^ /ifeOPTIONAL: LENGTH (mmWEJGHT (g)

(25 SPECIMEN MA3: SUBS AMPLE)

FISH SAMPLING-HELD DATA SHEET (FRONT)of ?

STREAM NAME 5-yWfs C'tefc

STATION * 5O-.3 RJVERMD-I

] .AT LONG

STORET*

GEAR

FORM COMPLETED BY

LOCATION

STREAM CLASS

RIVER BASIN

AGENCY

INVESTIGATORS

DATE f-l+O"! REASON FOE. SURVEY

TIME AM PM

SAMPLECOLLECTION

fa*l

HABITAT TYPES

GENERALCOMMENTS

How were the fish captured? D back pack D tois barge D other


Sampling Duration Sfflrttjrns End nrnt DuratiDT)

Stream width On meters') Mas: Meari

Indicate the percentage of each habitat type presentD Riffles % D Pools % D Rims % D Snaes %D Submerged Macroribvtss % D Other ( ) %

G»-kt»r 4/v{ A'fy f>oM fi&0 jb*f »t-Mf ff i iflKliss l*s*e

OPTIONAL: LENGTH (mm)nVEIGHT (g)SPEOMEN MAX SUBSAMPLEl

Rauid Bioassessmenl Protocols' For Use in Sireams and Wadeabic River: Pennhvior..

FISH SAMPLING-FIELD DATA SHEET (FRONT)paê ^ of J>

STREAM NAME S46«.h C'ctk-

STATI OK! £ B 5 ' 3 RA/ERMILE

LAT LONG

STORE! *

GEAR

FORM COMPLETED BY

LOCATION

STREAM CLASS

RIVER BASIN

AGENCY

INVESTIGATORS

DATE f-36~~o*l SEASON FOX SURVEY

TIME AM PM

SAMPLECOLLECTION

fa* 1

HABITAT TYPES

GENERALCOMMENTS

Bow were the fish captured?


Sampling Duration Start cm:

D back pack D tote bargt

DNO

End tirat

D other

Duration

Stream width On meters) Ma:: Mean

Indicate the percentage of eachD RiSlEE % D PoolsD Submersed MacTOtibvtes

1

habitat type present% D Rims % D Snaps

% D Other ! )• %

%

S te»-=£--£*a*vfe r <7i 7 f- l^tafy/'5p&&rt ,

Scv&k chr&f

OPTIONAL: LENGTH (mm)AVElGHT ig)(25 SPECIMEN MAX SUBSAMPLE)

Ravul Bioassessmeni Protocols For Use in Sirecnns and Wadeabic River: Periphywr,, benlhic

FISH SAMPLING FIELD DATA SHEET (FRONT)of

STREAM NAME S),* & Orfe k LOCATION $ev*>ft-{j b~r^f>

STATION* 5 D-J3 RJVERMILE | STREAM CLASS

LAT LONG

STORET #

GEAR

FORM COMPLETED BY ( m C

RIVER BASIN tfô 0lou*<r>

AGENCY (J15 FP&

INVESTIGATORS bmC

DATE S'g'jrtTIME "*£ AM u0

y HP I^Cy ^,,w».REASON 7DP>. SURVF1'

/ftf3 Esl 1 TiSSut *<* ~>fi^j

SAMPLECOLLECTION

$^* <?

HABITAT TYPES

GENERALCOMMENTS

How were tnt fish captured? D back pad; &tbtt bargt D other

Block nets used? D YEE IBIÔ x/J

Sampling Duration Siarttmt IZ<^ End tims /3.'3^ Duration TÂ'/t^

Stream width (in meters) Ma:: ' / ' Mean O

Indicate the percentage of each habitat typt presentGMOffles JT % Eaôolr â % »BTlims 5 % D Snags • %D Submerged MacrcmbvteE % D Other i 1 %

OPTIONAL: LENGTH (mm)AVElGHT (gj(25 SPECIMEN MAX SUBSAMPLEl

-ir>r,vve«nriPni Llrr>in'~nh rnr I)ar. in Sireams and Waafiahip. Kiwr.r f-rrrnirvjm-

FISH SAMPLING -FIELD DATA SHEET (FRONT)of

STREAM NAME 54om}s (Ve ^

STATION* fiD-3 RJVERMILE

LAT LONG

STORET #

GEAR

FORM COMPLETED BY IT\

F"

LOCATION

STREAM CLASS

RIVER BASIN

AGENCY

INVESTIGATORS

DATE S-'tt-rf BEASON FDR SURVEY

TIME ~JT Q™ DJ-). £ty/7> c ^

SAMPLECOLLECTION

l f U / * 3

HABITAT TYPES

GENERALCOMMENTS



Sampling Duration Start time

D bade pack Q-TBie barge D other

QH^T

t < \ ' - 6 t " Endmnt /'I 2i Duration (~> <^>\^

Stream width On meters] Max Mean

Indicate the percentage of eachD Riffles % D PoolsD Submerged Macropiivtes

habitat typt present% D Huns % D Snags • %

% D Other f 1 %


w fl 1/Jt'nfian n (/> f-.'T\if

JFISH SAMPLING -FIELD DATA SHEET (FRONT)of

STREAM NAME jvf^ /r /\cd-

STATION £ k b -3 RJVERMELE

LAT LONG

STORST#

GEAR

FORM COMPLETED BY

| LOCATION

STREAM CLASS

RTVER BASIN

AGENCY

INVESTIGATORS

DATE S^£-cSTIME Îf AM PM

REASON POU SURVFi1

A ?ySAMPLECOLLECTION

IU ^3

HABITAT TYPES

GENERALCOMMENTS

How were tht fish captured?

Block nets used? DYES

Sampling Imration Start nm:

D back pad;

D NO

Stream width On meters) Maj:

Indicate the percentage of eachD Riffle: % D PoolsD Submerps'd Macrophvtes

D TDK bargt D othei

End time DuratQL /?*•">•

Mean

habitat type present% DRuns % O Snaps • %

% D Other f ) %

OPTIONAL: LENGTH (mm).'WEIGET (gj(25 SPECIMEN MAX SDBSAMPLE)

Rjnnr?r.-?-mfni rnr Use in Sireams and Wadeabic Rivcrr PwinhvTm-

PRELIMINARY SCAUE READIMG SHEET y -

COLUCTIOW DATE ^RIVER wets*SPECIES

I- READER _£

2^ READER, _

/ OF

DATE

DKTE

RSH

IB LEK6TH RftOtUS

mm

1C m 1ST 30LSCftLERftOlU*.

AGE1 I FwL

U>IW*»J'

REMARKS

/SB XL 7 7" 20OO

to/ l -7)

3637

c

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5/7 I

PRELIMINARY SCALE READIMQ SHEET

COLLECTION DATER l V E R METER

SPECIES

I- READER2^ READER

PAGE / OF /

DATE

DATE

RSH

ID

.1urr

SCflLE

RMXOS

ANU. KtÛREMEMTS SCW.e , mm, )

IE mMEANSCftLE

AGE2 |FmAL

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9.1 21

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17

PRELIMINARY SCALE REAWMQ SHEET

COLLECTION DATE

RIVER &;#;• ME^ 5fan£0'3SPECIES

1S-T

2^ READER

PAGE / OP /

DATE

DATE

RSH

UH6TH WT

SCftLC

fUttos

AMU. (e.«a«> , mm,

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AGE1 Z FmAi

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( OC

53

,. IS

{,2-

re?71

U 70

-1

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: [J,iafV^ R/CA*,,^ h-» Job #/Task/Labor Code: 2^/^3,^ /

Location:" 5Û/S ^ - /Q***//* jQ»».p .Sample JD: /g/p * / .- ^Coll. Date: y-gp-^ ; ID Time Budget:

Prelim. Sorter: /flffC Split Sorter: Presort ID Time: /^ Date:Subsample: Q-ffone D Abundant Taxa: Split: Split ID Time: —• Date: - Py:

-DRBPIII: D100 D 200 D 300 (Spec, subsamp.) Chiro. ID Time: Date: — By:" . ' . . .,(__ of grids) : :QG.Info: D Sort QC,and/orDJD.QC

: Q Other: :ID QC Time: _____ Date:' ' By:

QC. Taxonomic , Total Presort SplitCheck Order Taxon "Number = Number 4 Number Notes <m.=.aottQCH«ibir)

16

20

21 .22_

23 .24 .25_

26 .27 _

28 _

29

30

31

32 _

33

34

35

•3

/)/t*r.^~-*r • /Q ' ^ _^ . /^L,,r.

* /ASaS

Comments.

Client:,Location:_

Coll. Date:'

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET

v£LJ,*~P

•77

Prelim. Sorter: Split Sorter:Subsample: GMfone D Abundant Taxa: Split:

D RBP III: D 100 D 200 D 300 (Spec. Subsamp.)( of grids)

: D Other:

Job #/Task/Labor Code:' :SampleID: ^

ID Time Budget: •£.£>Presort ID Time: /,g Date^

Split ID Time: -Chiro. ID Time:

en

Date; -Date:

:QG Info: D Sort QC,and/or D ID QC• ID QC Time: Date:' By:

QC TaxonomicCheck Order Taxon

Total Presort Split'Number = Number + Number Notes Qnc. Sort QCHuntw)

-7— / f) A1 / r/<r/«^r r~/e/9fC/V/ 1 1 J F I'A'ar*i /it •

Z 3<&" <<r uJ^Lrr^

E

6_

7_

B_

S_

10_

11

/ yivi>/ rorr/>? /

' ( hl lSMO

if

14

20 _

21

22

23

24

26 _

27 _

2B_

29 _

30 _

31 .32 .33 .

34

35

Comments:/) I

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient:

Location:Col!. Date:

\l [

Prelim. Sorter: Split Sorter:Split:Subsample: £h None D Abundant Taxa:

Q RBP 111: D 100 D 2QD D 300 (Spec. Subsamp.)

: • • • • : : • • •;. \( :-of grids) ;toother:

Job #/Task/Labor Code:Sample ID: fc£) -^

ID Time Budget:Presort ID Time: / . p Date^

Split ID Time:Chiro. ID Time:

DateDate

:'QG Info: -D Sort QC, and/or DID QCQCTime: Date:'

By:By:'By:,

By:

QC .• TaxonomicCheck Order Taxon

Total Presort Split-Number = Number + Number Notes {Inc. Son QC Numbtri

3

4

5•

6_

7

8

9

10

11

14

15•

16

18

19•

20_

21 _

22.-23_

24 _

25_

26

27 _

28 .29 .30 _

31 _

32

33

34

35

Comments:

Page:. of /

Client:location:

Coll. Date:

Prelim. Sorter:

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEET_ Job #/Task/Labor Code:_ Sample JD:

ID Time Budget: ^2. oPresort ID Time: /,$-""

_ Split ID Time:Chiro. ID Time:

Split Sorter: _Subsample: Q-ffone D Abundant Taxa: Split:

QRBPIII: D100 D200 D 300 (Spec.Subsamp.)- (__ of grids)

D Other:

Date:QC.Info: D SortQC.and/or D ID QC

ID QC Time: Date:' By:

QC.: TaxonomicCheck Order Taxon

Total Presort Split'Number = Number 4 Number Notes <\ne. Sort QCNumbvl

2

3

A

B

B

7

B

B

10

Llr

t .'

13

14

15

16

17

t gu

19

20_

21 _

22_

23_

24 _

25 _

26 _

27 .28 _

29 _

30

31

32 _

33

34

35

Comments:I !

Client:Location:

Coll. Date:WL/T

<"- 27 -o <rCr

/• £**,£ ^L**s>f

Preiim. Sorter:

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETJob #/Task/Labor Code:

Sample ID:

ID Time Budget: £a?Presort ID Time:


Split Sorter: ?.oSplit:Subsample: Erflbne Q Abundant Taxa:

DRBPIII: 0100 D 200 0 300 (Spec. Subsamp.). ' . ' . ' . -.(__ of grids)

O Other

Date^Date:_Date"

-QC .Info: D Sort QC.and/or D .ID QCID QCTime: Date:" 'By:


Total Presort Split•Number = Number + Number Notes

i _

2_

3

4

5

6

7

8

e10

1V_

12_

13

14_

15_

16

oTf 3

AK

/

( Lr)

ko^ so

20 _

21 _

22_

23 _

24 _

25_

26 _

27 _

28 .

29 _

30

31

32 _

33

34

35

Comments: g£*2 ' M;l ^.L &fc^ S2j2*t~~~Page: f of /

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: \j ,',

Location: ^4rColl. Date:

. f n rv\ - Job #/Task/Labor

C \L

Prelim. Sorter: _ Split Sorter:Subsample: KjNone D Abundant Taxa:

•DRBP

Q Other

Split:01 00 D 200 D 300 (Spec. Subsamp.)

- . (__ _ of grids)

ID Time Budget:Presort ID Time: /. p


'QC.Info: D Sort QC.and/or D ID QCl D Q C T i m e : D a t e f B y :

QC :

CheckTaxonomic

Order

TotalTaxon Number =

PresortNumber j

Split

i- Number Notes One. Bait QC Number)

<^fAC 'lYfTfcL/ZJL Û.vôoû^ 4 - ""

rr

E

7

B_

9

10

11

13 _

14

20_

21-22_

23 _

24 .25 _

26 _

27 _

2B

29 _

30

31 _

32 _

33

34

35

Comments: *a<?LS ( 01* rr!>£i.

Page: / of


Client: \/ta(V •' &!en»!*b~ /k?*~r _ Job ft/Task/Labor Code: lotoi,cnj I <*>'Location: V , & ' (' w Sample ID:

Coll. Date: - r^pW ~ " ID Time Budget: <2.0Prelim. Sorter: Jffjfe Split Sorter: — Presort ID Time: Date: By:_

Subsample: Bfxlone D Abundant Taxa: Split: Split ID Time: Date; By:'DRBPIII: Q100 D20D Q30D (Spec.subsamp.) Chiro. ID Time: Date: By:'

'. '.. ' . ' -..( of grids) QG Info: D Sort QC. and/or DID QCD Other:__ ID QC Time: Date:' By:

QC .•• • • TaxonomicCheck Order Taxon

Total Presort SplitNumber = Number + Number Notes flnc.BoitacNumb.r)

1 T^Mfi /s n ,,r (?,fc*A 3 ^ 3— - . . /

n . .

3 f)(^4. df(rr,t^/« • ?!? frZ /)d /t,~.dr/ /&£s

4 " Vc

7

B

B

17

18

19

20 _

21 -22_

23 _

24

25_

26 _

27 _

2B_

2B_

3D_

31 _

32 _

33

34

35

L..LLM

Comments:

INVERTEBRATE SECTION LABORATORY IDENTIFICATION BENCH SHEETClient: ]/i*s<n~ -&/*>»fa fr* Job #/Task/Labor Code: -20f0\, &> / &

location: Z"A«/z <^/, -' /ynut/rr /L^S> Sample JD:Coll. Date: f

Prelim. Sorter: Split Sorter:Split:Subsampie: D None Q Abundant Taxa:

DRBPIII: Q100 D20D D300 (Spec.Subsamp.). - . • • - • •-.( -o f grids)

D Other:

ID Time Budget:Presort ID Time:


Date:_Date:Date7"

By:.By:..By:.

:QG.Info: D Sort QC.and/orO JD QCID QC Time: Date:' ' By:


Total Presort SplitNumber = Number 4 Number Notes (Inc. Sort QCNumbv)

2

3

4

5

6

7

11

16

21

23 _

24 _

25_

26 _

27-2B_

2B_

3D

31 _

32 .

33.

34

35

7 •' 7

Comments:


-Locaflon:

Coll. Date:Oi o t V?

Prelim. Sorter: Split Sorter: _Subsamplef EGNone D Abundant Taxa: Split:

D RBP III: D 100 D 200 D 300 (Spec. Subsamp.)

-.(__ -of grids)X9 Other . blOrAgLcK /"jowtje w i-Q

Job #/Task/LaborSample ID:

ID Time Budget:Presort ID Time: /, Q


*Date:DaterDate:

QC. TaxonomicCheck Order

By:..By:.

QC.Info: D Sort QC,and/or D JD QCID QC Time: _ __ Date:' 'By:

TaxonTotal Presort Split

•Number = Number + Number Notes rtnc.SortDCNnmb€r)

m. s v/r Kft i

)i D tF rrx. III)

I ' Kf J«t

_kti_int_

JHf II

1 1 - • • • • • • . • • • • • • • . '

12 famc~ (LobLoottytx O^^b; ro jp W-A.i3/%)0n^, rtr*~\by\\Qb36~ i"7AMO>r>^Xvîo//«_C_

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/ - 1 -/ ' 1' ' 1

/ - ) '

1 5 . . . . . .

16 /

1B.19_

21 _

22_

23_

24

25_

26

27 .28.29.30

31.

32 _

33

34

35

<,K -mxfrrrv&ri

Ol J

Comments: n>

Page: / of /._

NORMANDEAU ASSOCIATES, INC. EPA Region 5 Records Ctr ...

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