Molecular Science in Oral-Systemic Medicine ~Winter Seminar~ · 2013-01-28 · Molecular Science in Oral-Systemic Medicine - Winter Seminar - February 3rd~4th, 2013 2013.2.3(Sun)
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Molecular Science in Oral-Systemic Medicine
~Winter Seminar~
東京医科歯科大学
第8回グローバルCOE国際シンポジウム
東京医科歯科大学
Tokyo Medical and Dental UniversityThe 8th Global COE International Symposium
February 3rd~4th, 2013Tokyo Medical andDental University
http://www.tmd.ac.jp/cmn/gcoe/index.html
http://www.tmd.ac.jp/cmn/gcoe/index.html
グローバルCOEプログラム歯と骨の分子疾患科学の国際教育研究拠点
デント・メドミクスのインテリジェンスハブ
第8回グローバルCOE国際シンポジウム
Molecular Science in Oral-Systemic Medicine ~Winter Seminar~
Sixth Retreat Meeting
Molecular Science in Oral-Systemic Medicine - Winter Seminar -
February 3rd~4th, 2013
2013.2.3(Sun) Periodontal Medicine
9:00 − 9:10 PresidentAddress Takashi Ohyama
Session 1 Chairpersons : Denis F. Kinane, Yuichi Izumi
9:10 − 10:40 Steven Offenbacher AnUpdateinPeriodontalMedicine:ManagingOralInfectionsto ImproveOverallHealth
10:40 − 12:10 Panos N. Papapanou PeriodontalInfectionsandCardiovascularDisease:BiologicalPlausibility andEpidemiologicEvidence
Session 2 Chairpersons : Steven Offenbacher, Yasushi Furuichi
13:00 − 14:30 Denis F. Kinane TheRoleofHostResponseVariationsinOralandSystemicDisease Interactions
14:30 − 15:10 Fusanori Nishimura ThePeriodontalHostResponsewithType2Diabetes
15:10 − 15:50 Shogo Takashiba PeriodontalMedicine −PredictionofSystemicDiseasesonPeriodontalInfection−
15:50 − 16:20 PosterSession3(SixthRetreatMeeting)CoffeeBreak
Session 3 Chairpersons : Mark Bartold, Shinya Murakami
16:20 − 17:00 Yasushi Furuichi AssociationbetweenPeriodontalDiseaseandAdversePregnancy Outcome
17:00 − 17:40 Koji Inagaki InterrelationshipsbetweenSystemicOsteoporosisandPeriodontal Disease:AssociationandMechanisms
18:30 − Reception
2013.2.4(Mon) Advanced Therapy in Periodontic
Session 4 Chairpersons : Panos N. Papapanou, Koji Inagaki
10:00 − 11:30 Mark Bartold IsThereaRoleforStemCellsinPeriodontalRegeneration?
11:30 − 12:10 Shinya Murakami PeriodontalTissueEngineering −TheFuturePerspectiveofCytokineTherapyandCellTherapy−
12:10 − 12:50 Yuichi Izumi ClinicalApplicationofEr:YAGLaserinPeriodontaland Peri-implantTherapy
12:50 − ClosingRemark YuichiIzumi Adjourn
C O N T E N T SSteven Offenbacher ………………………………………………………… 1AnUpdateinPeriodontalMedicine:ManagingOralInfectionstoImproveOverallHealth
Panos N. Papapanou ………………………………………………………… 5PeriodontalInfectionsandCardiovascularDisease:BiologicalPlausibilityandEpidemiologicEvidence
Denis F. Kinane………………………………………………………………… 9TheRoleofHostResponseVariationsinOralandSystemicDiseaseInteractions
Fusanori Nishimura………………………………………………………… 13ThePeriodontalHostResponsewithType2Diabetes
Shogo Takashiba…………………………………………………………… 17PeriodontalMedicine−PredictionofSystemicDiseasesonPeriodontalInfection−
Yasushi Furuichi …………………………………………………………… 21AssociationbetweenPeriodontalDiseaseandAdversePregnancyOutcome
Koji Inagaki………………………………………………………………… 25InterrelationshipsbetweenSystemicOsteoporosisandPeriodontalDisease:AssociationandMechanisms
Mark Bartold ……………………………………………………………… 29IsThereaRoleforStemCellsinPeriodontalRegeneration?
Shinya Murakami…………………………………………………………… 33PeriodontalTissueEngineering−TheFuturePerspectiveofCytokineTherapyandCellTherapy−
Yuichi Izumi………………………………………………………………… 37ClinicalApplicationofEr:YAGLaserinPeriodontalandPeri-implantTherapy
Poster Session……………………………………………………………… 42
1
An Update in Periodontal Medicine: Managing Oral Infections to Improve Overall Health
Steven Off enbacher D.D.S., Ph.D., M. MSc.
ProfessorandChair,DepartmentofPeriodontology,UniversityofNorthCarolinaatChapelHill
PeriodontalMedicinewasdefinedin1996astheeffectofperiodontalinfectionsonsystemichealth.Thisdisciplinewasinitiallyestablishedtostudytheobservationsthatlinkedperiodontaldiseasewithanincreasedriskforheartattack,strokeandpretermdeliveryamongpregnantwomen.Althoughthisisanemergingfield,currentfindingssuggestthatperiodontitisisassociatedwithmoressevereandprevalentcardiovasculardisease,kidneydisease,chronicobstructiveairwaydisease,diabetesandpregnancycomplications.Theseassociationsappeartoconferadditionalriskevenafteradjustmentsforrelevantconfounderslikesmoking,obesity,andothertraditionalriskfactors.Currentevidencesuggestthattheperiodontalorganismsgainaccesstothesystemiccirculationandthatperiodontalinfectionscreateanoralwoundthatservesasaportalforthesystemicdisseminationoforalpathogens.Onceintothebloodstreamtheseorganismselicithepaticinflammation,vasculardamage,andmanyappearcapableofcrossingtheplacentalbarrierinpregnantwomen.Thus,clinicalperiodontaldiseaserepresentsaninfectedwoundthatcanserveasachronicportalforthesystemicdisseminationoforalpathogensthathasthepotentialtomediateinflammatorydamagetomultipleorganssystems.Theevidencefromhumansandanimalstudieswouldsuggestthatonceperiodontalorganismsenterintothecirculatorysystem,theyinduceupstreamanddownstreammediatorsofthehepaticacutephaseresponseresultinginincreasesinserumbiomarkerssuchassICAM(solubleintracellularadhesionmolecule-1),IL-6(interleukin6),fibrinogenandCRP(C-ReactiveProtein).Manyoftheseinflammatorymarkersarepredictiveofcardiovascular,pregnancyanddiabetesrisk.Itappearsthat,periodontaldiseasemay,inpart,explaintheassociationbetweentheobservedincreasesininflammatorybiomarkersoftheacutephaseresponse,coagulationandvascularstressasassociatedwithincreasedriskforcertainsystemicconditions. Duringpregnancy,periodontalinfectionalsoappearstobeaspecialthreattotheplacentaandfetus.Case-controlandprospectivecohortstudieshavesuggestedthatthereisasignificantassociationbetweenmaternalperiodontaldiseaseandpregnancycomplicationsthatresultinpretermdelivery[gestationalage(GA)<37weeks]andlowbirthweight[LBW,birthweight<2500g].Severalstudiessuggestthatperiodontaldiseaseindependentlyenhancestheriskofobstetriccomplicationsincludingpretermbirth,growthrestriction[birthweightlessthan10thpercentileofweightforgestationalage,adjustingforbabygenderandrace],preeclampsiaandverypretermbirth[deliverylessthan32weeksgestation].Periodontaldiseaserepresentsariskfortheseadversepregnancyoutcomesevenafteradjustingforarangeofpotentialconfoundersincludingprevioushistoryofpretermbirth,parity,maternalweightgain,smoking,andotherinfections.Datasuggestthatmaternalperiodontalinfectionsmaypotentiallyrepresentabonafideriskfactorforpretermbirthandgrowthrestrictionbyservingasaninfectiousandinflammatoryexposuretotheplacentaandfetusduringpregnancy.Thisinfectiousandinflammatorystresswhichrepresentsthetoxicityoftheoralbiofilmappearstoincreaseneonatalmorbidityandmayhavelife-longeffectsonthehealthoftheoffspring. Estimatesofthepotentialmagnitudeoftheeffectofperiodontaldiseaseontheseconditionsaresignificant,increasingtheriskasmuchastraditionalriskfactorssuchassmoking.Furthermore,datafromanimalmodelsofdiseaseprovideacompellingdemonstrationofbiologicalfeasibility.However,datasupportingthereversibilityofthisassociationinhumansisequivocal.Althoughsomeearlytreatmentstudiesshowpromise,otherstudiesdonotshowanysystemicbenefitsfromtreatment.Clearlyadditionalstudiesexploringthepotentialbenefitsoftreatingperiodontaldiseasetoreducingtheriskforthesesystemicconditionsareneeded.However,sinceperiodontaldiseaseisbothapreventableandtreatablecondition,itrepresentsapotentiallymodifiableriskfactor.Thus,ifperiodontaldiseaseisproventobecausallyrelatedtotheseconditions,thefindingscouldhaveatremendoushealthcareimpact.
2
Steven Offenbacher
CURRICULUM VITAE
Education
1972 BostonUniversityB.A.Chemistry Boston,Massachusetts1976 VirginiaCommonwealthUniversityD.D.S.Dentistry MedicalCollegeofVirginiaSchoolofDentistry1977 VirginiaCommonwealthUniversityPh.D.Biochemistry MedicalCollegeofVirginia GraduateSchoolofBasicSciences1980 HarvardMedicalSchoolM.MSc.OralBiology1980 HarvardSchoolofDentalMedicineCertificate
Periodontology DepartmentofPeriodontologyandOralMedicine1980 HarvardMedicalSchoolM.MSc.OralBiology
Position
1981-91 AssistantProfessorofBiochemistry GraduateDivisionofBiological&Biomedical Sciences SchoolofMedicine Dr.KeithWilkinson,Chairman1982-91 GuestResearcher,AnaerobicMicrobiology CentersforDiseaseControl1985-91 GuestResearcher,YerkesPrimateCenter Dr.HaroldMcClure,ChiefofPathobiology& Immunobiology1987-91 AssociateProfessorofPeriodontology/Oral Biologywithtenure SchoolofPostgraduateDentistry1988-91 AssociateProfessorintheWinshipCancer Center1991-94 AssociateProfessorofPeriodontology Director,ClinicalResearchUnit1994-03 ProfessorofPeriodontology Director,ClinicalResearchUnit2003-present OraPharmaDistinguishedProfessorof PeriodontalMedicine2010 InterimChair,DepartmentofPeriodontology2010-present Chair,DepartmentofPeriodontology
Award and Honors
2006 NortonM.RossAwardforExcellenceinClinical Research2006 AAP,ClinicalResearchAward2007 DistinguishedScientistHonoree,NewYork University.2008 AAP,ClinicalResearchAward2008 AAPEducatorAwardforOutstandingTeaching andMentoringinPeriodontics2008 AlumniStarAward,VirginiaCommonwealth University2009 AAP,ClinicalResearchAward2010 Classof58Award,ClinicalRelevanceofResearch
Publications
1. BoggessKA,EspinolaJA,MossK,BeckJ,OffenbacherS,CamargoCA.VitaminDstatusandperiodontaldiseaseamongpregnanwomen.JPeriodontol.2010Sep1[Epubaheadofprint].
2. SuwatanapongchedP,SuraritR,SrisatjalukR,OffenbacherS.TranslocationofPorphyromonasgingivalisinfectedmonocytesandassociatedcellularresponses.AsianPacJAllergyImmunol.2010;28:181-188.
3. BobetsisYA,BarrosSP,LinDM,ArceRM,OffenbacherS.Alteredgeneexpressioninmurineplacentasinaninfection-inducedintrauterinegrowthrestrictionmodel;amicroarrayanalysis.JReprodImmunol.2010Jun;85(2):140-148.
4. CardJW,CareyMA,VoltzJW,BradburyJA,FergusonCD,CohenEA,SchwartzS,FlakeGP,MorganDL,ArbesSJJr,BarrowDA,BarrosSP,OffenbacherS,ZeldinDC.ModulationofallergicairwayinflammationbytheoralpathogenPorphyromonasgingivalis.InfectImmun.2010Jun;78(6):2488-2496.
5. BarrosSP,WirojchanasakS,BarrowDA,PanagakosFS,DevizioW,OffenbacherS.Triclosaninhibitionofacuteandchronicinflammatorygenepathways.JClinPeriodontol.2010May:37(5):412-418.
6. OffenbacherS,BarrosS,MendozaL,MaurielloS,PreisserJ,MossK,deJagerM,AspirasM.Changesingingivalcrevicularfluidinflammatorymediatorlevelsduringtheinductionandresolutionofexperimentalgingivitisinhumans.JClinPeriodontol.2010Apr;37(4):324-333.
7. FisherEL,MossK,OffenbacherS,BeckJD,WhiteJrRP.Thirdmolarandnon-thirdmolarcariesexperienceinmiddle-agedAmericans,aprevalencestudy.JOralandMaxillofacialSurg.2010Mar;68(3):634-640.
8. HortonAL,BoggessKA,MossKL,JaredHL,BeckJ,OffenbacherS.Periodontaldisease,oxidativestress,andriskforpreeclampsia.JPeriodontol.2010Feb;81(2):199-204.
9. FriedewaldVE,KornmanKS,OffenbacherS,BeckJ,TaylorGW,BorgnakkeWSLettertotheEditor:Authors'response.JPeriodontol.2010Feb;81(2):199-204.
10.BlakeyGH,GeleskoS,MarcianiRD,HaugRH,OffenbacherS,PhillipsC,WhiteJrRP.ThirdmolarsandperiodontalpathologyinAmericanadolescentsandyoungadults:aprevalencestudy.JOralMaxillofacSurg.2010;68:325-329
11.ArceRM,DiazPI,BarrosSP,GallowayP,BobetsisY,ThreadgillD,OffenbacherS.CharacterizationoftheinvasiveandinflammatorytraitsoforalCampylobacterrectusinamurinemodeloffetoplacentalgrowthrestrictionandintrophoblasticcultures.JReproductiveImmunology.JofReproductiveImmunology.2010;84:145-153.
12. ZhangS,BarrosSP,NiculescuMD,MorettiAJ,OffenbacherS.AlterationofPTGS2promotermethylationinchronicperiodontitis.JDentRes.2010Feb;89(2):133-137.
13.HickmanMA,BoggessKA,MossKL,BeckJD,OffenbacherS.Maternalperiodontaldiseaseisassociatedwithoxidativestressduringpregnancy.AmJPerinatol.
3
2011Mar;28(3):247-252.14. PreisserJS,SenPK,OffenbacherS.Multiplehypothesis
testingforexperimentalgingivitisbasedonWilcoxonSignedRankStatistics.AmericanStatisticalAssociationStatisticsinBiopharmaceuticalResearch.2011:3(2).
15.XiongX,BuekensP,GoldenbergRL,OffenbacherS,QianX.Optimaltimingofperiodontaldiseasetreatmentforpreventionofadversepregnancyoutcomes:beforeorduringpregnancy?AmJObstet&Gynecol.2011Mar16.
16.BellKP,PhillipsC,PaquetteDW,OffenbacherS,WilderRS.Incorporatingoral-systemicevidenceintopatientcare:practicebehaviorsandbarriersofNorthCarolinadentalhygienists.JDentHyg.2011:85(2):88-113.
17.BarrosSP,ArceRM,GallowayP,LawterJR,OffenbacherS.TherapeuticeffectofatopicalCCR2antagonistoninducedalveolarbonelossinmice.JPeriodontalRes.2011;46:246-251.
18.GaraasR,MossKl,FisherEL,WilsonG,OffenacherS,BeckJD,WhiteRPJr.Prevalenceofvisiblethirdmolarswithcariesexperienceorperiodontalpathologyinmiddle-agedandolderAmericans.JOralMaxillofacSurg.2011Feb;69(2):463-470.
19.WhiteRPJr.,FisherEL,PhillipsC,TuckerM,MossKL,OffenbacherS.Visiblethirdmolarsasriskindicatorforincreasedperiodontalprobingdepth.JOralMaxillofacSurg.2011Jan;69(1):92-103
20. PerriR,NaresS,ZhangS,BarrosSP,OffenbacherS.MicroRNAmodulationinobesityandperiodontitis.JDentRes.2012;91(1):33-38.PMID:22043006.
21.FisherEL,GaraasR,BlakeyGH,OffenbacherS,ShugarsDA,PhillipsC,WhiteRPJr.Changesovertimentheprevalenceofcariesexperienceorperiodontalpathologyonthirdmolarsinyoungadults.JOralMaxillofacSurg.2012May;70(5):1016-1022.
22.BroderMB,SchwartzS,CarlsonJ,DibbleCF,KohitfarberH,OffenbacherS,BuseJB,BencharitS.Exploringsalivaryproteomesinedentulouspatientswithtype2diabetes.MolBiosyst.2012Apr;8(4):1304-1310.PMID:22314925.
23.KoromantzosPA,MakrilakisK,DerekaX,OffenbacherS,KatsilambrosN,VrotsosIA,MadianosPN.Effectofnon-surgicalperiodontaltherapyonC-reactiveprotein,oxidativestress,andmatrixmetalloproteinase(MMP)-9andMMP-2levelsinpatientswithtype2diabetes:arandomizedcontrolledstudy.JPeriodontol.2012;83(1):3-10.PMID:21627458.
24. SoutherlandJG,MossK,TaylorGW,BeckJD,PankowJ,GangulaPR,OffenbacherS.PeriodontitisanddiabetesassociationswithmeasuresofatherosclerosisandCHD.Atherosclorosis2012May;222(1):196-201.PMID:22440543.
25.BellKP,PhillipsC,PaquetteDW,OffenbacherS,WilderRS.Dentalhygienists’knowledgeandopinionsoforal-systemicconnections:implicationsforeducation.JDentEduc.2012Jun;76(6):682-694.PMID:22659696.
26.MatulaK,RamamurthyR,BoseC,GoldsteinR,CouperD,Peralta-CarcelenM,StewartD,GustafsonKE,Offenbacher
Steven Offenbacher
S.Effectofantenataltreatmentofmaternalperiodontitisonearlychildhoodneurodevelopment.AmJPerinatol.2012Jul6;29(10):815-822.PMID:22773286.
27.DivarisK,MondaKL,NorthKE,OlshanAF,LangeEM,MossK,BarrosSP,BeckJD,OffenbacherS.Genome-wideassociationstudyofperiodontalpathogencolonization.JDentRes.2012Jul;91(7Suppl):21S-28S.PMID:22699663.
28.OffenbacherS,BeckJD,MossKL,BarrosS,MendozaL,WhiteRPJr.Whatarethelocalandsystemicimplicationsofthirdmolarretention?JOralMaxillofacSurg.2012Sep;70(9Suppl1):S58-65.PMID:22916700.
29.TaxmanDJ,LeiY,ZhangS,Holley-GuthrieE,OffenbacherS,TingJP.Asc-dependentRIP2kinaseregulatesreducedPGE2productioninchronicperiodontitis.JDentRes.2012Sep;91(9):877-82.PMID:22928789.
30.TaskForceforThirdMolarSummary.Summaryofthethirdmolarclinicaltrials;reportoftheAAOMStaskforceforthirdmolarsummary.JOralMaxillofacSurg.2012Sep;70(9):2238-2248.PMID:2290711.
31.ArceRM,CaronKM,BarrosSP,OffenbacherS.Toll-likereceptor4mediatesintrauterinegrowthrestrictionaftersystemicCampylobacterrectusinfectioninmice.MolOralMicrobiol.2012Oct;27(5):373-381.PMID:22958386.
32.BencharitS,AltarawnehSK,BaxterSS,CarlsonJ,RossGF,BorderMB,MackCR,ByrdWC,DibbleCF,BarrosS,LoewyZ,OffenbacherS.Elucidatingroleofsalivaryproteinsindenturestomatitisusingaproteomicapproach.MolBiosyst.2012Oct30;8(12):3216-3223.PMID:23041753.
33.WehmeyerMMH,KshirsagarAV,BarrosSP,BeckJD,MossK,PreisserJ,OffenbacherS.ArandomizedcontrolledtrialofintensiveperiodontaltherapyonmetabolicandinflammatorymarkersinpatientswithESRD:resultsofanexploratorystudy.
34.Al-TarawnehSK,BencharitS,MendozaL,CurranAE,BarrowDA,BarrosSP,PreisserJ,OffenbacherS,LoewyZ,GendreauL.ClinicalandhistologicalfindingsofdenturestomatitisasrelatedtointraoralcolonizationpatternsofC.albicans,salivaryflowanddrymouth.JProsthodont.2012Oct25.[Epubaheadofprint].PMID:23107189.
35. ZhangS,BarrosSP,MorettiAJ,YuN,ZhouJ,PreisserJS,OffenbacherS.EpigeneticregulationofTNFAexpressioninperiodontaldisease.JPeriodontol.2012Nov25.[Epubaheadofprint].
36.BarrosSP,Al-TarawnehSK,BencharitS,LoewyZ,GendreauL,OffenbacherS.Salivarycytokineandlevelsindenturestomatitis:anexploratorycase-controlstudy.OpenJStomatology,2012Dec.
37.OffenbacherS,BeckJD,BarrosSP,SurukiR,LoewyZG.Obstructivelungdiseaseandedentulismintheatherosclerosisriskincommunities(ARIC)study.BMJOpen2012Dec19;2:e001615.doi:10.1136/bmjopen-2012-001615.
4
M E M O
5
Periodontal Infections and Cardiovascular Disease : Biological Plausibility and Epidemiologic Evidence
Panos N. Papapanou, D.D.S., Ph.D.
ProfesserandChair,SectionofOralandDiagnosticSciences,ColumbiaUniversityCollegeofDentalMedicine
Oneofthekeyfeaturesofperiodontaldiseasesistheintimatecontactbetweenthebiofilm
ofthemicrobialplaqueandtheulceratedepitheliumoftheperiodontalpocket.Thisallows
thesubgingivalbacteria,someofwhichhavetissue-invadingproperties,togaindirectaccess
totheunderlyingconnectivetissueandgingivalmicrovasculaturethroughthedisrupted
epithelialbarrier.Asaresult,bacteremiasarefairlycommoninpatientswithperiodontitis
andaretriggeredbymechanicalstimulationofthegingivaltissues.Inaddition,inflammatory
mediatorsthatareabundantlyproducedlocallyintheinflamedgingivathroughcellularinnate
andadaptiveimmunitypathwayscanenterthebloodstream,reachdistantorgansandalso
‘excite’thevascularendothelium.Thesephenomenahavebeenshowntobeimportantinthe
contextofatherogenesis.Thepresentationwillgiveanoverviewofthepotentialbiologically
plausiblemechanismsthroughwhichperiodontalinfection/inflammationmayleadtoextra-oral
pathology,andsummarizetheavailableepidemiologicevidencefromcross-sectional,cohortand
interventionstudiesthathaveinvestigatedtheassociationofperiodontitisandcardiovascular/
cerebrovasculardisease.Thepublichealthimplicationsofthesefindingswillbediscussed.
6
CURRICULUM VITAE
Education
1979-1984 SchoolofDentistry,UniversityofAthens, Greece D.D.S,awardedJuly19841985-1989 Ph.DProgram,FacultyofOdontology,Göteborg University,Sweden Ph.D.inPeriodontics,awardedDecember1989 Thesistitle:Patternsofalveolarbonelossin theassessmentofperiodontaltreatment priorities.Sponsor:Prof.JanL.Wennström SwedishDentalJournal,Supplement66,19892001 ColumbiaUniversitySchoolofDentalandOral Surgery,NewYork,NY D.D.S.,awardedMay2001Position
1992-1995 AssociateProfessor DepartmentofPeriodontology,Göteborg University1995-1998 AssociateProfessor DepartmentofOralMicrobiology,Göteborg University1997-1998 AssistantDean,ResearchGraduateCurriculum FacultyofOdontology,GöteborgUniversity1998-2003 AssociateProfessorofDentistry ColumbiaUniversity,SchoolofDentalandOral Surgery1999-present Director,DivisionofPeriodontics, ColumbiaUniversityCollegeofDentalMedicine2003-present ProfessorofDentalMedicine ColumbiaUniversityCollegeofDentalMedicine2003-present Chairman,SectionofOralandDiagnostic Sciences, ColumbiaUniversityCollegeofDentalMedicine2005 UniversityTenure
Award and Honors
1996 The1stAnthonyA.RizzoYoungInvestigatorAward; PeriodontalResearchGroup;InternationalAssociation forDentalResearch1998 TheWalther-EngelPrize; AcademyofGraduateDentalEducation,Karlsruhe, Germany2007 ClinicalResearchAward; AmericanAcademyofPeriodontology2007 EducatorAwardforOutstandingTeachingand MentoringinPeriodontics; AmericanAcademyofPeriodontology2009 SunstarWorldPeriodontalResearchAward SunstarFoundationforOralHealthPromotion2012 SpecialCitation,AmericanAcademyofPeriodontology
Publications
1. Dye,B.A.,Herrera-Abreu,M.,Lerche-Sehm,J.,Vlachojannis,C.,Pikdoken,L.,Pretzl,B.,Schwartz,A.,Papapanou,P.N.(2009)Serumantibodiestoperiodontalbacteriaasdiagnosticmarkersofperiodontitis.JournalofPeriodontology80:634-647.
2. Behle,J.H.,Sedagathfar,M.H.,Demmer,R.T.,Wolf,D.L.,Celenti,R.,Kebschull,M.,Belusko,P.B.,Herrera-Abreu,M.,Lalla,E.,Papapanou,P.N.(2009)Heterogeneityofsystemicinflammatoryresponsesafterperiodontaltherapy.JournalofClinicalPeriodontology36:287-294.
3. Michalowicz,B.S.,Hodges,J.S.,Novak,M.J.,Buchanan,W.,DiAngelis,A.J.,Papapanou,P.N.,Mitchell,D.A.,Ferguson,J.E.,Lupo,V.R.,Bofill,J.,Matseoane,S.(2009)ChangeinperiodontitisduringpregnancyandriskofpretermbirthandlowbirthweightJournalofClinicalPeriodontology36:308-314.
4. Ebersole,J.L.,Novak,M.J.,Michalowicz,B.S.,Hodges,J.S.,Steffen,M.J.,Ferguson,J.E.,DiAngelis,A.J.,Buchanan,W.,Mitchell,D.A.,Papapanou,P.N.(2009)Systemicimmuneresponsesinpregnancyandperiodontitis:Relationshiptopregnancyoutcomesintheobstetricsandperiodontaltherapy(OPT)studyJournalofPeriodontology80:953-960.
5. Noble,J.M.,Borrell,L.N.,Papapanou,P.N.,Elkind,M.,Scarmeas,N.,Wright,C.(2009)Periodontitisisassociatedwithcognitiveimpairmentamongolderadults:analysisofNHANESIIIJournalofNeurologicalandNeurosurgicalPsychiatry80:1206-1211.
6. Papapanou,P.N.,Behle,J.H.,Kebschull,M.,Celenti,R.,Wolf,D.L.,Handfield,M.,Pavlidis,P.,Demmer,R.,(2009)Subgingivalbacterialcolonizationprofilescorrelatewithgingivaltissuegeneexpression.BMCMicrobiologyOct18,9(1):221.
7. Michalowicz,B.S.,Novak,M.J.,Hodges,J.S.,DiAngelis,A.J.,Buchanan,W.,Papapanou,P.N.,Mitchell,D.A.,Ferguson,J.E.,Lupo,V.R.,Bofill,J.,Matseoane,S.,Steffen,M.,Ebersole,J.F.(2009)Seruminflammatorymediatorsinpregnancy:Changesfollowingperiodontaltreatmentandassociationwithpregnancyoutcomes.JournalofPeriodontology80:1731-1741.
8. Demmer,R.T.,Papapanou,P.N.,Jacobs,D.R.Jr.,Desvarieux,M.(2009)Evaluatingclinicalperiodontalmeasuresassurrogatesforbacterialexposure:TheOralInfectionsandVascularDiseaseEpidemiologyStudy(INVEST).BMCMedicalResearchMethodology,Jan7;10:2.
9. Pollreisz,A.,Huang,Y.,Roth,G.A.,Cheng,B.,Kebschull,M.,Papapanou,P.N.,Schmidt,A.,Lalla,E.(2010)Enhancedmonocytemigrationandpro-inflammatorycytokineproductionbyPorphyromonasgingivalisinfection.JournalofPeriodontalResearchSeptember45:239-245.
10.Demmer,R.T.,Papapanou,P.N.(2010)Epidemiologicpatternsofchronicandaggressiveperiodontitis.Periodontology200053:28-44.
11.Desvarieux,M.,Demmer,R.T.,Jacobs,D.R.,Rundek,T.,
Panos N. Papapanou
7
Boden-Albala,B.,Sacco,R.L.,Papapanou,P.N.(2010)Periodontalmicrobiotaandhypertension.TheOralInfectionsandVascularDiseaseEpidemiologyStudy(INVEST).JournalofHypertension28:1413-1421.
12.Vlachojannis,C.,Dye,B.A.,Herrera-Abreu,M.,Pikdöken,L.,Lerche-Sehm,J.,Pretzl,B.,Celenti,R.,Papapanou,P.N.(2010)DeterminantsofserumIgGresponsestoperiodontalbacteriainanationallyrepresentativesample.JournalofClinicalPeriodontology37:685-696.
13.Kebschull,M.,Demmer,R.T.,Papapanou,P.N.(2010)“Gumbugleavemyheartalone”-Epidemiologicandmechanisticevidencelinkingperiodontalinfectionsandatherosclerosis.JournalofDentalResearch89:879-902.
14.Kebschull,M.,Papapanou,P.N.(2010)Theuseofgenearraysindecipheringthepathobiologyofperiodontaldiseases.MethodsinMolecularBiologyIn:MoleculartechniquesandapplicationsinOralBiology,666:385-393.
15.DemmerR.T.,Pavlidis,P.,Papapanou.P.N.(2010)Bioinformaticstechniquesinmicroarrayresearch:AppliedmicroarraydataanalysisusingRandSASsoftware.MethodsinMolecularBiologyIn:MoleculartechniquesandapplicationsinOralBiology,666:395-417.
16. Pollreisz,A.,Hudson,B.I.,Chang,J.S.,Qu,W.,Cheng,B.,Papapanou,P.N.,Schmidt,A.M.,LallaE.(2010)Receptorforadvancedglycationendproductsmediatespro-atherogenicresponsestoperiodontalinfectioninvascularendothelialcells.Atherosclerosis212:451-456.
17.Albert,D.A.,Begg,M.D.,Andrews,H.F.,Williams,S.Z.,Ward,A.,Conicella,M.L.,Rauh,V.,Thomson,J.L.,Papapanou,P.N.(2011)Anexaminationofperiodontaltreatment,dentalcare,andpregnancyoutcomesinaninsuredpopulationintheUnitedStates.AmericanJournalofPublicHealth101:151-156.
18.Kebschull,M.,Papapanou,P.N.(2011)Periodontalmicrobialcomplexesassociatedwithspecificcellandtissueresponses.JournalofClinicalPeriodontology38:supplement11:17-27.
19.Michalowicz,B.,Hodges,J.,Lussky,R.,Bada,H.,Rawson,T.,Buttross,L.,Chiriboga,C.,DiAngelis,A.,Novak,M.,Buchanan,W.,Mitchell,D.,Papapanou,P.N.(2011)Theinfluenceofmaternalperiodontitisoninfantneurodevelopment.Pediatrics127:e1212-1220.
20. Jönsson,D.,Ramberg,P.,Demmer,R.T.,Kebschull,M.,Dahlén,G.,Papapanou,P.N.(2011)Gingivaltissuetranscriptomesinexperimentalgingivitis.JournalofClinicalPeriodontology38:599-611.
21. Lalla,E.&Papapanou,P.N.(2011)Diabetesmellitusandperiodontitis:ataleoftwocommoninterrelateddiseases.NatureReviewsEndocrinology7:738-748.
22.Messier,M.D.,Emde,K.,Stern,L.,Radhakrishnan,J.,Vernocchi,L.,Cheng,B.,Angelopoulos.C.Papapanou,P.N.(2012)Radiographicperiodontalbonelossinchronickidneydisease.JournalofPeriodontology83:602-611.
23.Albert,D.A.,Ward,A.,Allweiss,P.,Graves,D.T.,Knowler,
W.C.,Kunzel,C.,Leibel,R.L.,Novak,K.F.,Oates,T.W.,Papapanou.P,N.,Schmidt,A,M,,Taylor,G.W.,Lamster,I.B.,Lalla.E.(2012)Diabetesandoraldisease:implicationsforhealthprofessionalsAnnalsoftheNewYorkAcademyofSciences1255:1-15.
24. Lockhart,P.B,Bolger,A.F.,Papapanou,P.N.,Osinbowale,O.,Trevisan,M.,Levison,M.E.,Taubert,K.A.,Newburger,J.W.,Gornik,H.L.,Gewitz,M.H.,Wilson,W.R.,Smith,S.C.Jr.,Baddour,L.M.(2012)PeriodontalDiseaseandAtheroscleroticVascularDisease:DoestheEvidenceSupportanIndependentAssociation?:AScientificStatementFromtheAmericanHeartAssociation.Circulation125:2520-2544.
25. Papapanou,P.N.(2012)TheprevalenceofperiodontitisintheUS:Forgetwhatyouwretold.JournalofDentalResearch91:907-908.
26. Stoecklin-Wasmer,C.,Guarnieri,P.,Celenti,R.,Demmer,R.T.,Kebschull,M.,Papapanou,P.N.(2012)MicroRNAsandtheirtargetgenesingingivaltissues.JournalofDentalResearch91:934-940.
27. Papapanou,P.N.,Trevisan,M.(2012)Periodontitisandatheroscleroticvasculardisease:Whatweknowandwhyitisimportant.JournaloftheAmericanDentalAssociation143:826-828.
28.Demmer,R.T,Squillaro,A.,Papapanou,P.N,Rosenbaum,M.,Friedewald,W.T.,Jacobs,D.R.Jr.,Desvarieux,M.(2012)Periodontalinfection,systemicinflammationandinsulinresistance:ResultsfromtheContinuousNationalHealthandNutritionExaminationSurvey(NHANES)1999-2004.DiabetesCare35:2235-2242.
29. Papapanou,P.N.(2012)TheprevalenceofperiodontitisintheUS:forgetwhatyouweretold.JournalofDentalResearch91:907-908.
30.Acomparisonofperiodontalstatusintworegional,population-basedstudiesofSHIPandINVEST(2012)Holfreter,B.,Demmer,R.T.,Bernhardt,O.,Papapanou,P.N.,Schwahn,C.,Kocher,T.,Desvarieux,M.JournalofClinicalPeriodontology39:1115-1124.
31. IdeM,Papapanou,P.N.(2013)Epidemiologicalevidenceforassociationsbetweenmaternalperiodontaldiseaseandadversepregnancyoutcomes–asystematicreviewJournalofClinicalPeriodontology40:inpress.
32.Krämer,B.,Kebschull,M.,Nowak,M.,Demmer,R.T.,Haupt,M.,Körner,C.,Perner,S.,Jepsen,S.,Nattermann,J.,Papapanou,P.N.(2013)RoleoftheNKcell-activatingreceptorCRACCinperiodontitisInfectionandImmunity81:inpress.
33.Bostanci,N.,Ramberg,P.,Wahlander,Å.,Grossman,J.,Jönsson,D.,Barnes,V.,&Papapanou,P.N.(2013)Label-freequantitativeproteomicsrevealsdifferentiallyregulatedproteinsinexperimentalgingivitis.JournalofProteomeResearch12:inpress.
Panos N. Papapanou
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The Role of Host Response Variations in Oral and Systemic Disease Interactions
Denis F. Kinane, B.D.S., Ph.D.
MortonAmsterdamDeanProfessorofPathologyandPeriodontologyUniversityofPennsylvania,SchoolofDentalMedicine
Variationsinhostresponseamongsthumansarecriticalindeterminingwhogetsdiseaseandwhoremainshealthygiventhesameenvironmentalchallenges.Clearlyinitiatingandenvironmentalchallengesarenecessaryinanydisease,andinperiodontaldiseasethesubgingivalbiofilmandchallengessuchassmokingcanfulfilltheseroles.Thislecturewillexploretherelationshipbetweenoraldisease,specificallyperiodontaldisease,andotherchronicinflammatorydiseasessuchasdiabetes,heartdiseaseandpre-termpregnancies.Thecentralroleofthechronicinflammatoryburdenasalinkingfeaturebetweenthesediseasesandthespecificpotentialeffectoftheperiodontalbiofilmasthemajoretiologicalagentwillbeaddressed.Mechanisticlinks,particularlyforcardiovasculardisease,andtheconceptofsharedriskfactorswillbecoveredalso.Thegatekeeperroleofoursurfaceepitheliallayeranditsvariationswillbeexploredinbothitsmolecularvariationsandfunctions.StimulationofTLRsbyperiodontalbacteriatypicallyproduceseitherahyper-inflammatoryresponseorananti-microbialpeptideresponse.Theseresponsesareunderstoodtoberegulatedbyepigeneticmodifications.LikewisethechangesincellularreceptorexpressionaremediatedbymicroRNAswhichmayalsohavearoleinapoptoticresponses.Featuressuchasthelipidraftcellularreceptors,inparticulartheTLRfamilyaswellasGPCRswillbereviewedaswillthepotentialforsignalingthroughthesereceptorsinproducingcytokine,andothercellularinflammatoryandanti-inflammatoryresponses.Theimportanceofgenetics,specificallybySNPcarriage,andtheregulationofinflammationbybothmicroRNAaffectsandbychromatinremodelingandotherepigeneticchangeswillbementioned.Thecentralroleofchronicinflammationandhowitcontributesandisworsenedinavarietyofstateswillbeconsidered.Theaudienceshouldachieveanunderstandingandarealisticperspectiveoftheinteractionsofthesechronicdiseasesandknowledgeontheirprevention.
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Denis F. Kinane
CURRICULUM VITAE
Education
1980 BDSUniversityofEdinburgh1983 PhDDepartmentofMicrobiology,Universityof
Edinburgh,MedicalSchool1986 FDSRCSRoyalCollegeofSurgeonsEdinburgh1993 FDSRCPSRoyalCollegeofPhysiciansandSurgeons
GlasgowPosition
1980-1983 ResearchFellow,MicrobiologyDepartment, EdinburghUniversityMedicalSchool1983-1983 HouseOfficeinOralMedicine/Periodontology, EdinburghDentalHospitalandSchool1983-1987 LecturerinPeriodontology,DundeeDental HospitalandSchool1988-1994 SeniorLecturer,HonoraryConsultantand CoordinatorofthePeriodontalUnit, UniversityofGlasgowDentalHospitaland School.1994 Additional-ProfessorinPeriodontologyand OralImmunology1995 Additional–Director,OralImmunology ResearchGroup(topranked,2001RAE)2000 Additional-AssociateDeanforResearchand Enterprise2002-2009 AssociateDeanResearch,EndowedProfessor, Dept.Periodontology,Professor,Dept. Microbiology&Immunology.Universityof LouisvilleDentalSchool,Kentucky.Since2009 MortonAmsterdamDean,Professorof PathologyandPeriodontology,University ofPennsylvaniaSchoolofDentalMedicine, Philadelphia,Pennsylvania,USA
Award and Honors
1. EuroPerioIResearchAward:Adonogianaki,Wennstrom,Kinane.1994
2. BritishSocietyofPeriodontology,Researchprize:Apatzidou,Kinane.2001
3.‘B’meritawardholder(NHS).20004. DeltaEndowedChair,UniversityofLouisville.20025. HonoraryMemberoftheSwissSocietyofPeriodontology.
20016. KYHEROSAwardtoenhancescientificappreciationinthe
community.20047. Chair-ElectoftheGordonConferenceonPeriodontology
2003-2008.8. CouncillorforthePeriodontalResearchGroupoftheInt.
Assoc.DentalResearch.9. BasicScienceAwardoftheInternationalAssociationfor
DentalResearch2012.
Publications
IL-6,tPA,PAI-2,albuminlevelsfollowinginitialperiodontal1.treatmentinchronicperiodontitispatientswithorwithouttype2diabetes.InflammRes.2011Feb;60(2):143-51.Epub2010Sep17.PubMedPMID:20845058.KardeşlerL,BuduneliN,ÇetinkalpS,LappinD,KinaneDF.GingivalcrevicularfluidOzçakaO,Ba2. şoğluOK,BuduneliN,TaşbakanMS,BacakoğluF,KinaneDF.Chlorhexidinedecreasestheriskofventilator-associatedpneumoniainintensivecareunitpatients:arandomizedclinicaltrial.JPeriodontalRes.2012Feb29.doi:10.1111/j.1600-0765.2012.01470.xKinaneJA,BenakanakereMR,ZhaoJ,HosurKB,Kinane3.DF.Porphyromonasgingivalisinfluencesactindegradationwithinepithelialcellsduringinvasionandapoptosis.CellMicrobiol.2012
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The Periodontal Host Response with Type 2 Diabetes
Fusanori Nishimura, D.D.S., Ph.D.
ProfesserandChair,DepartmentofDentalScienceforHealthPromotion,HiroshimaUniversityInstituteofBiomedicalandHealthSciences
Inthepastdecade,periodontaldiseasehasbeenrecognizedasnotmerelyalocalinfectiousdisease,butaschronic,subclinical,inflammatorydiseaseforthehost.Thesubjectswithtype2diabetesappeartorespondtobacterialchallengeinanexaggeratedmannerascomparedwiththesubjectswithoutdiabetesthroughseveralpossiblemechanisms,anddevelopsevererformsofinflammatoryperiodontaldisease.Severeperiodontaldiseaseinsuchsubjects,inturn,actstoreduceinsulinsensitivityknownasinsulinresistance,therebycontributingtotheinductionofhyperglycemiaaswellashyperinsulinemia,importantriskfactorsforvascularcomplications.Additionally,recentstudiessuggestedthatsuchsubclinicalinflammatorystatealsopromotesrenaldysfunction,allofwhichareimportantriskfactorsforatherosclerosis.Finally,allsuchconditionsacttoincreasetheriskforcoronaryheartdiseases,oneoftheleadingcausesofmortalityandmorbidityinsubjectswithdiabetes.Thus,itisveryimportanttoelucidatemolecularmechanismsastowhylocalperiodontalinflammationisamplifiedtothelevelofinfluencingouroverallsystemichealth. Recently,macrophageshavebeensuggestedtoinfiltrateintoadiposetissues,andtointeractwithadipocytes,therebyexacerbatingadiposetissueinflammation.Furthermore,bothcelltypesappeartoexpresstoll-likereceptor-4(TLR4),andfreefattyacidshavebeenfoundtoactasendogenousligandforTLR4.Basedonthesefindings,wehypothesizedthat,incasesofinfectiousdiseasessuchassevereperiodontaldiseaseandgutinfection,classicalexogenousligandforTLR4mayfurtherexacerbateinflammatoryresponsesinadiposetissue,therebycontributingtotheinductionofmanyunwantedsideeffectssuchasinsulinresistance.Toprovethis,weestablishedco-culturesystembetweenadipocytesandmacrophagesandstimulatedthesecellswithbacteriallipopolysaccharide(LPS).WefoundthatstimulationofthecellswithLPSmarkedlyup-regulatedinflammatorygeneexpressioninadipocytesaswellasproteinproductionsfromco-cultures.Someoftheseeffectswereconfirmedinvivomodelbyusingbothgeneticallyinducedandenvironmentallyinducedobesemodelmiceaswell.Hence,ourresultssuggestthat,althoughtheprevalenceofextremelyobesesubjectsasseeninwesternsocietiesisverylowineasternAsiaincludingJapan,oncetheydevelopsevereperiodontaldiseases,thediseasemayup-regulateadiposetissueinflammation,especiallyinsubjectswithnewlydiagnosedtype2diabeteswhoareslightlymoreobesethanthesubjectswithoutdiabetes.Currentunderstandingonthesemechanismswillbediscussedinthecontextofdevelopingnew
“order-made”diagnosticstrategiestoavoidsuchunwantedsideeffects.
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Fusanori Nishimura
CURRICULUM VITAE
Education
1985 DDS,KyushuUniversityFacultyofDentistry
Position
1988 Assistant,OkayamaUniversityDentalSchool1990 Post-DoctoralResearchFello,ColumbiaUniversity SchoolofDentalandOralSurgery1993 AssociateResearchScientist,ColumbiaUniversity SchoolofDentalandOralSurgery1995 Assistant,OkayamaUnivesityDentalSchool1997 AssistantProfessorOkayamaUniversityHospital2003 AssocateProfessor,OkayamaUniversityGraduate SchoolofMedicineandDentistry2006 Professor,HiroshimaUniversityGraduateSchoolof BiomedicalSciences
Award and Honors
1999 YoungInvestigatorAward,JapaneseSocietyOf ConservativeDentistry1999 TheFirstAwardInBasicResearchCategoryOf7th MeetingOfTheInternationalAcademyOf Periodontology2005 LionAward,JapaneseSocietyOfPeriodontology
Publications
1. IwataH,SogaY,MeguroM,YoshizawaS,OkadaY,IwamotoY,YamashitaA,TakashibaS,NishimuraF.Highglucoseup-regulateslipopolysaccharide-stimulatedinflammatorycytokineproductionviac-junN-terminalkinaseinmonocyticcelllineTHP-1.JEndotoxinRes,13:227-234,2007.
2. YamashitaA,SogaY,IwamotoY,YoshizawaS,IwataH,KokeguchiS,TakashibaS,NishimuraF.Macrophage-adipocyteinteraction:MarkedIL-6productionbyco-culturesstimulatedwithLPS.Obesity,15:2549-2552,2007.
3. YamashitaA,SogaY,IwamotoY,AsanoT,LiY,AbikoY,NishimuraF.DNAmicroarrayanalysesofgenesexpresseddifferentiallyin3T3-L1adipocytesco-culturedwithmurinemacrophagecelllineRAW264.7inthepresenceofthetoll-likereceptor4ligandbacterialendotoxin.IntJObese,32:1725-1729,2008.
4. NakaraiH,YamashitaA,NagayasuS,IwashitaM,KumamotoS,OhyamaH,HataM,SogaY,KushiyamaA,AsanoT,AbikoY,NishimuraF.Adipocyte-macrophageinteractionmaymediateLPS-inducedlow-gradeinflammation:potentiallinkwithmetaboliccomplications.InnateImmunity,[E-pubaheadofprint:2010]18:164-170,2012.
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Periodontal Medicine− Prediction of Systemic Diseases on Periodontal Infection −
Shogo Takashiba, D.D.S., Ph.D.ProfesserandChair,DepartmentofPathophysiology-PeriodontalMedicine,OkayamaUniversityGraduateSchoolofMedicine,DentistryandPharmaceuticalSciences
Epidemiologicalstudieshavesuggestedtherelationsamongmanysystemicdiseasesandperiodontitis.Inthosestudies,clinicalindiceshavebeenusedasparametertoshowseverityofperiodontitis.Whatisreallyinvolvedinthepathogenicityofthesystemicdiseasesthatperiodontitisrelates?Directpathogenicityofperiodontalbacteriaseemstobeasmallportionofthepathogenicitybetweenthesediseases.Hostresponsestothebacteriaordenaturedhostproteinsmustbealargeportionofpathogenicitysuchasimmuneresponsestomoleculesmimickedtothebacterialantigensorinvolvedininflammation. Forfurtherunderstandingtheseverityofperiodontitis,wehavebeenusedbothbacterialDNAtoidentifyspecificperiodontalbacteriaandimmunoglobulinG(IgG)todeterminethedegreeofsensitizationbybacterialantigens.IncreasedIgGantibodylevelmeansactivatedacquiredimmuneresponseandfurtherhostresponsethatmaycausefailureofhomeostasis,resultingtissueororgandamages.Realpathogenicityhasnotbeenwelldocumented. However,usingthesetwoparameters,wecanunderstandthehostresponsesofperiodontitispatients.Therearethreegroups;1)majorgroupwithregularIgGresponsereflectingtheseverityofperiodontalinfection,2)minorgroupwithhypo-response(lowIgGlevel)tosevereperiodontalinfection,3)minorgroupwithhyper-response(highIgGlevel)tolessperiodontalinfection. Inthefirstgroup,IgGantibodylevelchangesaccordingtotheamountofbacterialDNAdetectedfromperiodontallesion.Thus,itiseasytoinferaboutthesensitizationofhostfromtheselaboratoryexaminations.Furthermore,thisgroupmaybeclosetothosewhoseperiodontalconditionsrelatedtothesomesystemicdiseases.Studiesusingthisassumptionhavebeenconducted,suggestingrelationsbetweenperiodontitisandsystemicdiseasesomehow.Inthesecondgroup,IgGantibodylevelstayslowerleveleventhoughalotofamountofbacterialDNAdetectedfromperiodontallesion.Thehostmaybeinimmuno-compromisedcondition.Bacteremiaoraspirationpneumoniamustbeconsidered.Inthethirdgroup,IgGv\levelstayshigherleveleventhoughverylowamountofbacterialDNAdetectedfromperiodontallesion.Hyper-immunoglobulinemiathatmayrelatetoinjuryofglomerulusmustbeconsidered.Inaddition,excessiveimmunoreactionmayexistandinjuretissuesororgans.Becausefewstudiesusingthisassumptionhavebeenconducted,furtherstudiesmustbeperformedforthesesystemicdiseases. Inthislecture,Iwillshowourrecentstudiesasexamplesfortheideamentionedabove.ThedefinitionofthresholdofIgGtiteragainstPorphyromonasgingivalis ,utilizationoftheIgGtitertesttoexplainchronicobstructivepulmonarydisease,preliminarystudytorevealtherelationoftheIgGtiterandatherosclerosisrelatedtodiabetes.
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Shogo Takashiba
CURRICULUM VITAE
Education
1986 OkayamaUniversityDentalSchool1990 OkayamaUniversityGraduateSchoolofDentistryPosition
1990 AssistantProfessor OkayamaUniversityHospitalofDentistry1992 VisitingScientist EastmanDentalCenter(Rochester,NY,USA)1994 AssistantProfessor OkayamaUniversityDentalSchool1995 AssociateProfessor OkayamaUniversityDentalSchool2001 AssociateProfessor OkayamaUniversityGraduateSchoolofMedicineand
Dentistry2002 Professor OkayamaUniversityGraduateSchoolofMedicineand
Dentistry2005 Professor OkayamaUniversityGraduateSchoolofMedicine,
mDentistryandPharmaceuticalSciences
Award and Honors
1997 IADRTravelAward2001 JapaneseSocietyofPeriodontologyScienceAward
Publications
1. MyokaiF,etal.Anovellipopolysaccharide-inducedtranscriptionfactorregulatingtumornecrosisfactoralphageneexpression:molecularcloning,sequencing,characterization,andchromosomalassignment.ProcNatlAcadSciUSA.1999Apr13;96(8):4518-23.
2. OmoriK,etal.Highglucoseenhancesinterleukin-6-inducedvascularendothelialgrowthfactor165expressionviaactivationofgp130-mediatedp44/42MAPK-CCAAT/enhancerbindingproteinsignalingingingivalfibroblasts.JBiolChem.2004Feb20;279(8):6643-9.
3. YamazakiK,etal.Relationshipofperiodontalinfectiontoserumantibodylevelstoperiodontopathicbacteriaandinflammatorymarkersinperiodontitispatientswithcoronaryheartdisease.ClinExpImmunol.2007Sep;149(3):445-52.
4. MartinezZR,etal.Geneprofilesduringrootcanaltreatmentinexperimentalratperiapicallesions.JEndod.2007Aug;33(8):936-43.
5. YamaguchiT,etal.IL-6/sIL-6RenhancescathepsinBandLproductionviacaveolin-1-mediatedJNK-AP-1pathwayinhumangingivalfibroblasts.JCellPhysiol.2008Nov;217(2):423-32.
6. KitamuraM,etal.Periodontaltissueregenerationusingfibroblastgrowthfactor-2:randomizedcontrolledphaseIIclinicaltrial.PLoSOne.2008Jul2;3(7):e2611.
7. hematopoieticstemcelltransplantationward.SupportCareCancer.2010Feb;19(2):303-7.
8. SogaY,etal.Progressoforalcareandreductionoforalmucositis--apilotstudyina
9. KitamuraM,etal.FGF-2stimulatesperiodontalregeneration:resultsofamulti-centerrandomizedclinicaltrial.JDentRes.2011Jan;90(1):35-40.
10. SugiN,etal.PrognosisofperiodontitisrecurrenceafterintensiveperiodontaltreatmentusingexaminationofserumIgGantibodytiteragainstperiodontalbacteria.JClinLabAnal.2011;25(1):25-32.
11.TsugeS,etal.SpecificinsituvisualizationofplasmacellsproducingantibodiesagainstPorphyromonasgingivalisingingivalradicularcyst:applicationoftheenzyme-labeledantigenmethod.JHistochemCytochem.2011Jul;59(7):673-89.
12.NakaoR,etal.OutermembranevesiclesofPorphyromonasgingivaliselicitamucosalimmuneresponse.PLoSOne.2011;6(10):e26163.
13.TomikawaK,etal.Smad2deceleratesre-epithelializationduringgingivalwoundhealing.JDentRes.2012Aug;91(8):764-70.
14.TakahashiT,etal.Relationshipbetweenperiodontitis-relatedantibodyandfrequentexacerbationsinchronicobstructivepulmonarydisease.PLoSOne.2012;7(7):e40570.
15.KudoC,etal.Assessmentoftheplasma/serumIgGtesttoscreenforperiodontitis.JDentRes.2012Dec;91(12):1190-5.
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Association between Periodontal Disease andAdverse Pregnancy Outcome
Yasushi Furuichi, D.D.S., Odont.Dr.ProfesserandChair,DivisionofPeriodentology&Endodontology,DepartmentofOralRehabilitation,SchoolofDentistry,HealthSciencesUniversityofHokkaido
Periodontaldiseaseisaninfectiousdiseasewithacertainextentofinflammatorylesionintheperiodontiumwherenumbersofmicroorganismsandinflammatorycellsareincontactandvarietiesofinflammatorymediatorsareproducedand/orinfiltratedin.Chronicperiodontitisbecomesprominentatlateragesofadulthood,butgingivitisproceedsattheiryoungerages.Adversepregnancyoutcomeincludespre-termbirth(PB:deliveryearlierthan37weeksofgestation)andlowbirth-weight(LBW:deliveryweightlessthan2,500g).IncidencerateofPLBW(PBand/orLBW)arereportedtobe4~15%andvariesamongdifferentcountriesanddistricts.PLBWisthemaincauseofmortalityandmorbidityofthenew-bornandbeanineligibleburdennotonlyfromfamilialbutalsofromsocioeconomicaspects. Bothperiodontaldiseaseandadversepregnancyoutcomearemulti-factorialinitspathogenesisandpossesscommonriskfactorssuchassmoking.Sincethemiddleof1990`s,anumberofepidemiologicalandexperimentalstudieshavebeenperformedonthetopicoftheassociationbetweenperiodontaldiseaseandPLBWandtheresultswerereportedintheliterature.Suchstudiescanbecategorizedintothreegroupsaccordingtotheaimsofthestudies,i.e.analyzingtheassociationbetweenthebothevents,analyzingthemechanismsbehindtheassociation,andevaluatingtheeffectsofperiodontalinterventionontheassociation.Althoughtherehavebeensomewhatlargediscrepanciesintheresultsoftheassociationstudies,significantpositiveassociationshavebeenreportedinmorethanhalfofthelargescaledepidemiologicalstudies.Mechanismsoftheassociationhavebeenanalyzedwithemployingmicrobiological,immune-pathological,andgenomicmethods.Sofar,therehavebeenseveralstudiesshowinginterestingfindingswhichmayinpartexplainthemechanismsoftheassociation.ItcouldbeconcludedthatthebiggestcontroversieswerefoundintheperiodontalinterventionstudiesandthatperiodontaltreatmentswouldnotberecommendedduringthepregnancyinordertoreducetheincidencesofPLBWaccordingtotheresultsofthelargescaledRCTs.However,ithasalsobeenclaimedthatperiodontaltreatmentduringthepregnancyinitselfisbeneficialinordertotreatperiodontaldiseaseandmaintainhealthierperiodontiumofthepregnantwomen.ItwasalsodemonstratedthatunsuccessfulperiodontaltreatmentsresultedinhigherrisksofPLBWthansuccessfulperiodontaltreatmentsinastudy.Inthispresentation,associationbetweenperiodontaldiseaseandadversepregnancyoutcomewillbediscussedfromthethreeaspectsmentionedabove.
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Yasushi Furuichi
CURRICULUM VITAE
Education
HegraduatedfromtheFacultyofDentistry,KagoshimaUniversityin1985.HereceivedLicentiatedegreeinOdontologyin1992,andOdontDrdegreein1998,attheFacultyofOdontology,GothenburgUniversityinSweden,.Position
HeisaprofessorandchairoftheDivisionofPeriodontologyandEndodontology,DepartmentofOralRehabilitation,SchoolofDentistry,HealthSciencesUniversityofHokkaidosince2004,andisadirectoroftheDentalandMedicalClinicofthesameuniversitysince2009.HewasaprivatepractitioneratSakumaDentalClinicinIbaraki,Japanbetween1985-1988and1993-1993,andaresearchassistantattheDepartmentofPeriodontology,FacultyofOdontology,UniversityofGothenburg,Swedenbetween1988-1991and1993-1998.Hewasanassistantprofessorbetween1998-2000,alecturerbetween2000-2002,andanassociateprofessorbetween2002-2004attheDepartmentofPeriodontology,FacultyofDentistry,KagoshimaUniversity,Japan.
Publications
1. FuruichiY,ShimotsuA,ItoH,NamariyamaY,YotsumotoY,HinoY,MishigeY,InoueM,IzumiY.AssociationsofperiodontalstatuswithgeneralhealthconditionsandserumantibodytitersforPorphyromonasgingivalisandActinobacillusactinomycetemcomitans.JPeriodontol.2003;74(10):1491-7.
2. HasegawaK,FuruichiY,ShimotsuA,NakamuraM,YoshinagaM,KamitomoM,HataeM,MaruyamaI,IzumiY.Associationsbetweensystemicstatus,periodontalstatus,serumcytokinelevels,anddeliveryoutcomesinpregnantwomenwithadiagnosisofthreatenedprematurelabor.JPeriodontol.2003;74(12):1764-70.
3. NakajimaY,FuruichiY,BiswasKK,HashiguchiT,KawaharaK,YamajiK,UchimuraT,IzumiY,MaruyamaI.Endocannabinoid,anandamideingingivaltissueregulatestheperiodontalinflammationthroughNF-kappaBpathwayinhibition.FEBSLett.2006;580(2):613-9.
4. ShirakataY,SetoguchiT,MachigashiraM,MatsuyamaT,FuruichiY,HasegawaK,YoshimotoT,IzumiY.Comparisonofinjectablecalciumphosphatebonecementgraftingandopenflapdebridementinperiodontalintrabonydefects:arandomizedclinicaltrial.JPeriodontol.2008;79(1):25-32.
5. YonamineY,MatsuyamaT,SonomuraT,TakeuchiH,FuruichiY,UemuraM,IzumiY,NoguchiK.Effectableapplicationofvascularendothelialgrowthfactortocriticalsizedratcalvariadefects.OralSurgOralMedOralPatholOralRadiolEndod.2010;109(2):225-31.
6. NagasawaT,NodaM,KatagiriS,TakaichiM,TakahashiY,Wara-AswapatiN,KobayashiH,OharaS,KawaguchiY,TagamiT,FuruichiY,IzumiY.Relationshipbetweenperiodontitisanddiabetes-importanceofaclinicalstudytoprovetheviciouscycle.InternMed.2010;49(10):881-5.
7. HidakaT,NagasawaT,ShiraiK,KadoT,FuruichiY.FGF-2inducesproliferationofhumanperiodontalligamentcellsandmaintainsdifferentiationpotentialsofSTRO-1(+)/CD146(+)periodontalligamentcells.ArchOralBiol.2012;57(6):830-40.
8. KomatsuT,NaganoK,SugiuraS,HagiwaraM,TanigawaN,AbikoY,YoshimuraF,FuruichiY,MatsushitaK.E-selectinmediatesPorphyromonasgingivalisadherencetohumanendothelialcells.InfectImmun.2012;80(7):2570-6.
9. KatoS,NakashimaK,NagasawaT,AbikoY,FuruichiY.InvolvementofToll-likereceptor2inapoptosisofAggregatibacteractinomycetemcomitans-infectedTHP-1cells.JMicrobiolImmunolInfect.2012.
10.YamadaY,NakamuraS,ItoK,UmemuraE,HaraK,NagasakaT,AbeA,BabaS,FuruichiY,IzumiY,KleinOD,WakabayashiT.InjectableBoneTissueEngineeringUsingExpandedMesenchymalStemCells.StemCells.2012.
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Interrelationships between Systemic Osteoporosis and Periodontal Disease : Association and Mechanisms
Koji Inagaki, D.D.S., Ph.D.
Professor,DepartmentofDentalHygiene,AichiGakuinUniversityJuniorCollege
Manystudieshaveattemptedtodefinetherelationshipbetweenpostmenopausalosteoporosis
andperiodontaldisease.Moststudiessupportapositiveassociationbetweenthesecommon
diseases;however,manyarecross-sectionalinnature,includerelativelysmallsamplesizes,and
haveinadequatecontrolofpotentialconfoundingfactors,suchasage,gender,hormoneintake,
race,andsmoking,limitingourunderstandingofthenatureoftherelationshipbetweenthese
diseases.Clinicalconditionscausinglowestrogenenvironmentsinpostmenopausalwomen
allowT-andB-cellabnormalities,increasedlocalproductionofthebone-activecytokines(i.e.,
Interleukin-1,-6and-8,tumornecrosisfactor-(TNF-)andariseinprostaglandinE2,resulting
intheprogressionofperiodontitis.Inpost-menopausalosteoporosis,lackofestrogenwillaffect
theremodelingofthebonetissueinsuchawaythat,inmostpatientswithperiodontitis,the
amountofboneresorbedexceedsthatbeingformed,resultinginnetboneloss.Osteoporosis
canbetreatedbyavarietyofmethods,thehormonereplacementtherapy(HRT),theselective
estrogenreceptormodulators(SERM)andthebisphosphonates.TheHRTorbisphosphonates
treatmentsimprovetheclinicaloutcomeofperiodontaldiseaseandmaybeanadjunctive
treatmenttopreserveperiodontalbonemass.Thispresentationreviewsthecurrentevidence
onthemechanismofperiodontalbreakdownaftermenopausewithlong-termfollow-upcases
andthebenefittooralhealthbytreatmentsforosteoporosis.
26
Koji Inagaki
CURRICULUM VITAE
Education
1976-1982 Aichi-GakuinUniversityNagoya DDS1982-1986 Aichi-GakuinUniversity (graduatecourseinPeriodontology) PhDPosition
Professor,DepartmentofDentalHygiene,AichiGakuin1.UniversityJuniorCollegeAssociateProfessor,DepartmentofPeriodontology,Aichi2.GakuinUniversityDiplomateoftheJapaneseBoardofPeriodontology3.DiplomateoftheJapaneseBoardofTobaccoControl4.DiplomateoftheJapaneseBoardofConservativeDentistry5.DiplomateoftheJapaneseBoardofOcclusion&Health6.CaretakerofAichiAssociationforTobacco-freeKidsand7.ResearchGrouponSmoke-FreePsychologyDirectorofDiplomate,JapaneseSocietyofPeriodontology8.
Award and Honors
2003 TheIncentiveAwardoftheJapanOsteoporosisSociety2009 TheIncentiveAwardoftheTokaiSchoolHealth Association
Publications
MoritaI,NakagakiH,TaguchiA,KatoK,Murakami1.T,TsuboiS,HayashizakiJ,InagakiK,NoguchiT.:RelationshipsbetweenmandibularcorticalbonemeasuresandbiochemicalmarkersofboneturnoverinolderJapanesemenandwomen.OralSurgOralMedOralPatholOralRadiolEndod,108(5):777-783,2009OtaniT,YoshiiC,KanoM,KitadaM,InagakiK,Kurioka2.N,IsomuraT,HaraM,OkuboY,KoyamaH.:ValidityandreliabilityofKanotestforsocialnicotinedependence(KTSND).AnnEpidemiol,19(11):815-822,2009YoshiiS,TsuboiS,MoritaI,TakamiK,InukaiJ,InagakiK,3.NakagakiH.:TemporalassociationofelevatedC-reactiveproteinandperiodontaldiseaseinmen.JPeriodontol,80(5):734-739,2009KatagiriS,NittaH,NagasawaT,UchimuraI,Izumiyama4.H,InagakiK,KikuchiT,NoguchiT,KanazawaM,MatsuoA,ChibaH,NakamuraN,KanamuraN,InoueS,IshikawaI,IzumiY.:Multi-centerinterventionstudyonglycohemoglobin(HbA1c)andserum,high-sensitivityCRP(hs-CRP)afterlocalanti-infectiousperiodontaltreatmentintype2diabeticpatientswithperiodontaldisease.DiabetesResClinPract,83(3):308-315,2009DumitrescuAL,InagakiK.:Interrelationshipsbetween5.periodontaldiseaseandmortality,cardiovasculardisease,metabolicsyndrome,diabetesmellitus,DumitrescuAL.EtiologyandPathogenesisofPeriodontalDisease,1sted,Springer,Germany,2010,125-157,159-190,215-244,265-278,279-293,295-306,307-318.HuangB,InagakiK,YoshiiC,KanoM,AbbottPV,Noguchi6.T,TakahashiK,BesshoK.:SocialnicotinedependenceinAustraliandentalundergraduatestudents.IntDentJ,61(3):152-156,2011NomuraY,ShimadaY,HanadaN,NumabeY,KamoiK,7.SatoT,GomiK,AraiT,InagakiK,FukudaM,NoguchiT,YoshieH.:Salivarybiomarkersforpredictingtheprogressionofchronicperiodontitis.ArchOralBiol,57(4):413-420,2012MoritaI,InagakiK,NakamuraF,NoguchiT,Matsubara8.T,YoshiiS,NakagakiH,MizunoK,SheihamA,SabbahW.:Relationshipbetweenperiodontalstatusandlevelsofglycatedhemoglobin.JDentRes,91(2):161-166,2012NoguchiT,KikuchiT,InagakiK.:Periodontitisand9.diabetes,YoshieH,Attheforefront:illustratedtopicsindentalresearchandclinicalpractice,1sted,QuintessencePublishingCoInc,Germany,2012,43-46.
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Is There a Role for Stem Cells in Periodontal Regeneration?
Mark Bartold, D.D.S., Ph.D.
ProfessorofPeriodontics&DirectorColgateAustralianClinicalDentalResearchCenterUniversityofAdelaide
Althoughstemcellshavereceivedconsiderableattentioninrecentyears,clonogenicbonemarrowstromalstemcells(alsoknowasmesenchymalstemcells)werefirstisolatedandcharacterizedalmost40yearsago.Sincethenconsiderableefforthasbeenmadeindeterminingthetherapeuticapplicationsofmesenchymalstemcells.Mesenchymalstemcellpropertiessuchasmultipotencyandimmunomodulationmakethesecellsidealcandidatesfortissueregenerationandtissueengineering.Mostofusarefamiliarwiththeconceptofembryonicstemcellsandtheirpotentialuseintissueregeneration.Overthepast10years,reportshaveappearedcharacterizingmesenchymalstemcellsisolatedfromtheperiodontaltissues(periodontalligamentandgingiva)andtheirpotentialuseinperiodontalregeneration.Morerecentlyreportsofisolationofinducedpluripotentstem(iPS)cellsfromgingivaandperiodontalligamenthaveappeared.BothmesenchymalstemcellsandiPScellsfromperiodontaltissuesprovideanattractivesourceofcellsforperiodontaltissueengineering.Itisnowclearthatinorderfortissueengineeringtoreachitsfullregenerativepotentialanappropriatescaffoldforcelldeliverytogetherwiththeincorporationofsupplementalcytokinesandgrowthfactorswillbenecessaryfortheregenerativeprocesstoproceedbothspatiallyandtemporally.Thepreciserolesofmesenchymalstemcellsintissueregenerationstillneedtobeelucidated.Forexample,itisstillunclearwhetherthesecellsactviadirectdifferentiationinsituorthroughparacrinemediatedprocessesleadingtorecruitmentoflocalprogenitorcellstoenabletissueregeneration.Inthispresentationthebiologyandpotentialforclinicaluseofperiodontalstemcellsforperiodontalregenerationwillbeexplored.
30
Mark Bartold
CURRICULUM VITAE
Education
BDS,UniversityofAdelaide1.BScDent(Hons)universityofAdelaide2.PhD,UniversityofAdelaide3.FRACDS(Perio),RoyalAustralasianCollegeofDental4.SurgeonsDDSc,UniversityofAdelaide5.
Position
ProfessorofPeriodonticsDirectorColgateAustralianClinicalDentalResearchCenter
Award and Honors
1992 Who'sWhoinAustralia FirstlistedinWho'sWhoinAustraliafrom 1992-present PublishedbyInformationAustralia(ISBN0810-8226)1995 AcademyofDentistryInternational ElectedFellowoftheAcademyofDentistry International1995 AustralianDentalAssociation Appointed"GuestLecturer”totheNorthern Territory"for19951996 AlanDockingIADRScienceAward Awardedforoutstandingcontributionstodental science InternationalAssociationforDentalResearch Australian&NewZealandDivision Sydney,September19961998 InternationalCollegeofDentists ElectedFellowoftheInternationalAcademyof Dentists2002 RoyalAustralasianCollegeofDentalSurgeons MeritoriousServiceAward2003 PierreFauchardAcademy ElectedFellow2004 OrderofAustralia MemberoftheOrderofAustralia2005 IndianSocietyofPeriodontology HonoraryLifeMembership2008 MeritoriousServiceAward,AustralianDental Association Awardedforoutstandingservicetodentistry,dental educationandtheADAinAustraliainan extraordinarymannerfor30years.2012 RayWilliamsPrize,AustralianSocietyof Periodontology Forthemostsignificantseriesofpaperspublishedin periodontalresearchforthetriennium2009-2011.
Publications
1. GossAN,BartoldPM,SambrookP,HawkerP.ThenatureandfrequencyofbisphosphonateassociatedosteonecrosisofthejawsindentalimplantpatientsinSouthAustralia.JournalofOralandMaxillofacialSurgery68:237-243;2010.
2. FitzsimmonsTR,SandersAE,BartoldPM,SladeGD.Localandsystemicbiomarkersingingivalcrevicularfluidincreaseoddsofperiodontitis.JournalofClinicalPeriodontology37:30-36;2010.
3. BartoldPM,CantleyMD,HaynesDR.Mechanismsandcontrolofpathologicbonelossinperiodontitis.Periodontology200053:55-69;2010.
4. MrozikK,ZilmPS,BagleyCJ,HackS,HoffmanP,GronthosS,BartoldPM.Proteomiccharacterizationofmesenchymalstemcell-likepopulationsderivedfromperiodontalligament,dentalpulpandbonemarrow:analysisofdifferentiallyexpressedproteinsStemCellsandDevelopment19:1485-1499;2010
5. KatariaNG,BartoldPM,DharmapatniAASK,AtkinsGJ,HoldingCA,HaynesDR.Expressionoftumornecrosisfactorlikeweakinducerofapoptosis(TWEAK)anditsreceptorFN14inhealthyandperiodontitistissues.JournalofPeriodontalResearch45:564-573;2010
6. BartoldPM,Marino,V,Cantley,M,Haynes,DR.EffectofP.gingivalis-inducedinflammationondevelopmentofrheumatoidarthritis.JournalofClinicalPeriodontology37:405-411;2010
7. MenicaninD,BartoldPM,ZannettinoACW,GronthosS.Identificationofacommongeneexpressionsignatureassociatedwithimmatureclonalmesenchymalcellpopulationsderivedfrombonemarrowanddentaltissues.StemCells&Development19:1501-1510;2010
8. MegsonE,FitzsimmonsT,DharmapatniK,BartoldPM.C-reactiveproteiningingivalcrevicularfluidisindicativeofsystemicinflammation.JournalofClinicalPeriodontology37:797-804;2010.
9. MegsonE,KapellasK,BartoldPM.RelationshipbetweenPeriodontalDiseaseandOsteoporosis.InternationalJournalofEvidence-BasedHealthcare8:129-139;2010.
10.BartoldPM,KuliwabaJS,LeeV,ShahS,MarinoV,FazzalariNL.Influenceofsurfaceroughnessandshapeonmicrodamageoftheosseoussurfaceadjacenttotitaniumdentalimplants.ClinicalOralImplantsResearch22:613-618;2011.
11.GronthosS.,ArthurA.,BartoldPM.,andShiS.,AMethodtoIsolateandCultureExpandHumanDentalPulpStemCells.MesenchymalStemCellAssaysandApplications.In:MethodsinMolecularBiology.Editors:MohanC.Vemuri,MahendraS.Rao&LucasG.Chase.HumanaPressNJ,USA.698:107-121;2011.
12. ZilmPS,BartoldPM.ProteomicidentificationofproteinaseinhibitorsintheporcineEnamelMatrixDerivative,EMD®.JournalofPeriodontalResearch46:111-117,2011
13.WadaN,BartoldPM,GronthosS.HumanForeskin
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FibroblastsExertImmunomodulatoryPropertiesbyaDifferentMechanismtoBoneMarrowMesenchymalStemCells.StemCellsandDevelopment20:647-659;2011.
14.BatesCE,MarinoV,FazzalariNL,BartoldPM.Softtissueattachmenttotitaniumimplantscoatedwithgrowthfactors.ClinicalImplantandRelatedDentistry2011Mar22.doi:10.1111/j.1708-8208.2010.00327.x.[Epubaheadofprint]
15.WadaN,WangB,LinN-H,LaslettAL,GronthosS,BartoldPM.InducedpluripotentstemcelllinesderivedfromhumangingivalandperiodontalligamentfibroblastsJournalofPeriodontalResearch46:438-447;2011
16.CantleyMD,FairlieDP,BartoldPM,RainsfordKD,LeGT,LuckeAJ,HoldingCA,HaynesDR.InhibitorsofhistonedeacetylasesinclassIandclassIIsuppresshumanosteoclastsinvitroJournalofCellularPhysiology226:3233-3241;2011.
17.CantleyMD,HaynesDR,MarinoV,BartoldPM.Pre-existingperiodontitisexacerbatesrheumatoidarthritisinamousemodel.JournalofClinicalPeriodontology38:532-541;2011.
18.BartoldPM,MengH.AnAsianperspectiveofperiodontology.Periodontology200056:11-13;2011.
19.CantleyMD,BartoldPM,MarinoV,FairlieDP,LeGT,LuckeAJ,Haynes.DR.Histonedeacetylaseinhibitorssuppressbonelossinexperimentalperiodontitis.JournalofPeriodontalResearch46:697-703;2011.
20. SkiltonMR,Maple-BrownLJ,KapellasK,CelermajerDS,BartoldPM,BrownA,SladeGD,JamiesonLMTheeffectofaperiodontalinterventiononcardiovascularriskmarkersinIndigenousAustralianswithperiodontaldisease:thePerioCardiostudy.BMCPublicHealth2011,11:729
21. duBoisA,KardachiB,BartoldPM.Istherearolefortheuseofvolumetricconebeamcomputedtomography(CBVT)inperiodontics?AustralianDentalJournal57(suppl1):103-108;2012
22.ChooT,MarinoV,BartoldPMEffectofPDGF-BBandbeta-tricalciumphosphate( β -TCP)onboneformationarounddentalimplants:apilotstudyinsheep.ClinicalOralImplantsResearch(AcceptedforpublicationSeptember5,2011)
23.MrozikKM,GronthosS,MenicaninD,MarinoV,BartoldPM.EffectofcoatingStraumannBoneCeramicwithEmdogainonmesenchymalstromalcellhardtissueformation.ClinicalOralInvestigations16:867-878;2011.
24.ChanRC,MarinoV,BartoldPMEffectofEmdogain®andPlateletDerivedGrowthFactorontheOsteoinductivePotentialofHydroxyapatiteTri-CalciumPhosphate.ClinicalOralInvestigations16:1217-1227;2012.
25.CantleyM,BartoldPMFairlieD,RainsfordK,HaynesD.HistoneDeacetylaseInhibitorsassuppressorsofbonedestructionininflammatorydiseases.JournalofPharmacyandPharmacology64:763-774;2012
Mark Bartold
26.XiongJ,MrozikK,GronthosS,BartoldPM.EpithelialcellrestsofMalassezcontainuniquestemcellpopulationscapableofundergoingepithelial-mesenchymaltransition.StemCells&Development21:2012-2025;2012
27.HynesKE,MenicaninD,GronthosS,BartoldPMClinicalutilityofstemcellsforperiodontalregeneration.Periodontology200059:203-227;2012.
28.DoyleCJ,BartoldPM.Howdoesstressinfluenceperiodontitis.JournaloftheInternationalAcademyofPeriodontology14:25-32;2012.
29. ZahndG,VrayD,SérusclatA,AlibayD,BartoldM,BrownA,DurandM,JamiesonL,KapellasK,Maple-BrownL,O'DeaK,MoulinP,CelermajerD,SkiltonM.Longitudinaldisplacementofthecarotidwallandcardiovascularriskfactors:associationswithaging,adiposity,bloodpressureandperiodontaldiseaseindependentofcross-sectionaldistensibilityandintima-mediathickness.UltrasoundMedBiol.38:1705-1715;2012.
30.BartoldPM,VanDykeTE.Unlearninglearnedconcepts.Periodontitis–ahostmediateddisruptionofmicrobialhomeostasis.Periodontology2000(AcceptedMay30,2012).
31.GeS,MrozikKM,MenicaninD,StanGronthosS,BartoldPM.Isolationandcharacterizationofmesenchymalstemcellsfromhealthyandinflamedgingivaltissue:potentialuseforclinicaltherapy.RegenerativeMedicine(Acceptedforpublication25July2012)
32.HarveyG,FitzsimmonsT,DharmapatniK,MarchantC,HaynesD,BartoldPM.Expressionofpeptidylargininedeiminase–2and-4,citrullinatedproteinsandanti-citrullinatedproteinantibodiesinhumangingiva.JournalofPeriodontalResearchArticlefirstpublishedonline:16SEP2012|DOI:10.1111/jre.12002
33.AbbottJR,MarinoV,BartoldPMHumancadaverichistomorphologicalandmetallurgicalanalysisofdentalimplantsfollowing12.5yearsofservice.ClinicalOralImplantsResearch.AcceptedforpublicationOctober2,2012.
34.GannonSC,CantleyMD,HaynesDR,HirschR,BartoldPM.Azithromycinsuppresseshumanosteoclastformationandactivityinvitro.JournalofCellularPhysiologyAcceptedforPublicationOctober3,2012.
35.MarchantC,SmithMD,ProudmanS,HaynesDR,BartoldPM.EffectofPorphyromonasgingivalisoncitrullinationofproteinsbymacrophagesinvitro.JournalofPeriodontology(AcceptedforpublicationOctober17,2012).
36.XiongJ,GronthosS,BartoldPM.RoleofepithelialcellrestsofMalassezinthedevelopment,maintenanceandregenerationofperiodontalligamenttissues.Periodopntology2000(AcceptedforpublicationOctober31,2012)
37.WadaN,GronthosS,BartoldPM.ImmunomodulatoryEffectsofStemCellsPeriodontology2000(AcceptedforpublicationNovember1,2012)
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Periodontal Tissue Engineering − The Future Perspective of Cytokine Therapy and Cell Therapy −
Shinya Murakami, D.D.S., Ph.D.
ProfessorandChairman,DepartmentofPeriodontology,OsakaUniversityGraduateSchoolofDentistry
Ithasbeendemonstratedthatmesenchymalstemcellsandprogenitorcellsofosteoblastsorcementoblastscanbeidentifiedwithinperiodontalligament(PDL).Thus,improvingthebiologicalpotentialofthesecellsandstimulatingtheperiodontalregenerationarerecognizedasbeingclinicallypossible.Oneofthemostphysiologicallyefficientmethodstostimulatethesecellsistheuseofcytokines. BasisFibroblastGrowthFactor(FGF-2)isknowntobedeeplyinvolvedintheproliferation,migrationanddifferentiationofavarietyofcellsandtostronglyinduceangiogenesis.Utilizingbeagledogsandnon-humanprimates,werevealedthattopicalapplicationofrecombinantFGF-2inducedstatisticallysignificantperiodontaltissueregenerationintheexperimentally-preparedintraosseousbonedefects.Recently,ahumanclinicaltrialwasconductedusingFGF-2inJapan.Thiswasarandomizedcontrolleddouble-blindedclinicaltrialofdoseresponsesincludingplacebocomparison.Asaresult,asignificantdifferencein%increaseinalveolarboneheightat2-or3-walledintrabonydefectsofthepatientswasdemonstratedbystandardisedradiographsbetweenPlaceboGroupand0.3% -FGF-2Groupat9monthsafterthetreatment.ThissuggeststhattopicalapplicationofFGF-2canbeefficaciousinregenerationofperiodontaltissueofperiodontitispatients. Basedontheresultsofaseriesofinvitro analyses,wehavesuggestedthefollowingmodeofactionofFGF-2toinduceperiodontalregeneration.Duringtheearlystagesofperiodontaltissueregeneration,FGF-2stimulatestheproliferationandmigrationofPDLcellswhilemaintainingtheirmultipotentnature,inducingdifferentiationintohardtissue-formingcellssuchasosteoblastsandcementoblasts.Furthermore,FGF-2inducesangiogenesisandincreasesextracellular-matrixproductionsuchasosteopontin,hyaluronanfromPDLcells,thusleadingtoalocalenvironmentsuitablefortheperiodontalregeneration.TheseresultsintheenhancedperiodontaltissueregenerationareshownattheFGF-2-appliedsites. Foridealperiodontalregeneration,itiscrucialtofullyintroducetheconceptof“tissueengineering”.IfweneedtotreatseverebonydefectsorhorizontalbonedestructionwithFGF-2,itisessentialtointroducetheconceptofa“scaffold”intothecarrierofFGF-2drug.AnFGF-2carrierthatcouldprovideaformableandosteoconductivescaffoldforundifferentiatedprogenitorcellswithinPDLwoulddramaticallyincreaseboththedentalandcraniofacialapplicationsofFGF-2drug. Furthermore,researchers,includingourgroup,havefoundthatmesenchymalstemcellscanbeobtainedfromvarioustissuessuchasbonemarrowandadiposetissues.Byusingbeagledogmodels,werecentlyrevealedthattransplantationofadipose-tissuederivedstemcellsenhancesperiodontalregenerationatappliedsites.Thecombinedeffectsof‘celltherapy’and‘cytokinetherapy’needtobeassessedinthefuture,toallowtheestablishmentof‘periodontaltissueengineering’.
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Shinya Murakami
CURRICULUM VITAE
Education
1978-1984 D.D.S. OsakaUniversityFacultyofDentistry,Japan1984-1988 Ph.D. OsakaUniversityGraduateSchoolofDentistry, Japan
Position
1988-1988 ResearchFellow,DepartmentofPeriodontology andEndodontology,OsakaUniversityFaculty ofDentistry,Japan1988-1990 VisitingFellow,NationalCancerInstitute, NationalInstitutesofHealth,USA1990-1992 Instructor,DepartmentofPeriodontologyand Endodontology,OsakaUniversityFacultyof Dentistry1992-2000 AssistantProfessor,Departmentof PeriodontologyandEndodontology,Osaka UniversityDentalHospital2000-2002 AssociateProfessor,Departmentof Periodontology OsakaUniversityGraduateSchoolofDentistry2002-present ProfessorandChairman,Departmentof Periodontology,OsakaUniversityGraduate SchoolofDentistry2008-present ViceDirectorofOsakaUniversityDental Hospital
Award and Honors
1984 YumikuraAward:OsakaUniv.FacultyofDentistry1998 AnthonyRizzoPeriodontalResearchAward(IADR PeriodontalResearchGroup)2009 AAPR.EarlRobinsonPeriodontalRegenerationAward2012 IADR/AADRWilliamJ.GiesAward
Publications
S.Yamada,M.Tomoeda,Y.Ozawa,S.Yoneda,Y.1.Terashima,S.Ikegawa,M.Saito,S.Toyosawa,S.Murakami.PLAP-1/asporin:Anovelnegativeregulatorofperiodontalligamentmineralization.J.Biol.Chem.282:23070-23080,2007Y.Shimabukuro,T.Ichikawa,Y.Terashima,T.Iwayama,2.H.Oohara,T.Kajikawa,R.Kobayashi,H.Terashima,M.Takedachi,M.Terakura,T.Hashikawa,S.Yamada,S.Murakami.Basicfibroblastgrowthfactorregulatesexpressionofheparansulfateinhumanperiodontalligamentcells.MatrixBiology,27:232-241,2008Y.Terashima,YShimabukuro,H.Terashima,M.Ozasa,3.M.Terakura,K.Ikezawa,T.Hashikawa,M.Takedachi,H.Oohara,S.Yamada,S.Murakami.Fibroblastgrowthfactor-2regulatesexpressionofosteopontininperiodontalligamentcells.J.Cell.Physiol.216:640-650,2008M.Takedachi,QuD,EbisunoY,OoharaH,JoachimsML,4.McGeeST,MaedaE,McEverRP,TanakaT,MiyasakaM,MurakamiS,KrahnT,BlackburnMR,ThompsonLF.CD73-generatedadenosinerestrictslymphocytemigrationintodraininglymphnodes.J.Immunol.180:6288-6296,2008M.Kitamura,K.Nakashima,Y.Kowashi,T.Fujii,H.5.Shimauchi,T.Sasano,T.Furuichi,M.Fukuda,T.Noguchi,T.Shibutani,Y.Iwayama,S.Takashiba,H.Kurihara,M.Ninomiya,J.Kido,T.Nagata,T.Hamachi,K.Maeda,Y.Hara,Y.Izumi,T.Hirofuji,E.Imai,M.Omae,M.Watanuki,S.Murakami.Periodontaltissueregenerationusingfibroblastgrowthfactor-2Periodontaltissueregenerationusingfibroblastgrowthfactor-2:RandomizedControlledPhaseIIclinicaltrial.PLoSOne,3:e2611,2008M.Tomoeda,S.Yamada,H.Shirai,Y.Ozawa,M.Yanagita,S.6.Murakami.PLAP-1/asporininhibitsactivationofBMPreceptorviaitsleucinrichrepeatmotif.Biochem.Biophys.Res.Commun.371:191-196,2008.KomodaH,OkuraH,C.M.Lee,SougawaN,IwayamaT,7.HashikawaT,SagaA,Yamamoto-KakutaA,IchinoseA,MurakamiS,SawaY,andMatsuyamaA.ReductionofN-glycolylneuraminicacidxenoantigenonhumanadiposetissue-derivedstromalcells/mesenchymalstemcellsleadstosaferandmoreusefulcellsourcesforvariousstemcelltherapies.TissueEngPartA,16:1143-1155,2010CFujihara,SYamada,NOzaki,Ntakeshita,HKawaki,8.TTakano-Yamamoto,SMurakami.RoleofMechanicalstress-inducedglutamatesinaling-associatedmoleculesincytodifferentiationofperiodontalligamentcells.JBiolChem285:28286-28297,2010M.Kitamura,M.Akamatsu,M.Machigashira,Y.Hara,R.9.Sakagami,T.Hirofuji,T.Hamachi,K.Maeda,M.Yokota,
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J.Kido,T.Nagata,H.Kurihara,S.Takashiba,T.Shibutani,M.Fukuda,T.Noguchi,K.Yamazaki,H.Yoshie,K.Ioroi,T.Arai,T.Nakagawa,K.Ito,S.Oda,Y.Izumi,Y.Ogata,S.Yamada,H.Shimauchi,K.Kunimatsu,M.Kawanami,T.Fujii,Y.Furuichi,T.Furuuchi,T.Sasano,E.Imai,M.Omae,S.Yamada,M.Watanuki,andS.MurakamiFGF-2stimulatesperiodontalregeneration:Resultsofamulti-centerrandomizedclinicaltrial.JDentRes90:35-40,2011MurakamiS10.PeriodontalTissueRegenerationbysignallingmolecule(s):whatroledoesbasicfibroblastgrowthfactor(FGF-2)haveinperiodontaltherapy?Periodontology2000,56:188-208,2011ShimabukuroY,TerashimaH,TakedachiM,MaedaK,11.NakamuraT,SawadaK,KobashiM,AwataT,OoharaH,KawaharaT,IwayamaT,HashikawaT,YanagitaM,YamadaS,MurakamiSFibroblastgrowthfactor-2stimulatesdirectedmigrationofperiodontalligamentcellsviaPI3/AktsignalingandCD44/hyaluronaninteraction.J.Cell.Physiol.226:809-821,2010JAnzai,Mkitamura,TNozaki,TNagayasu,ATerashima,12.TAsano,SMurakamiEffectsofconcomitantuseoffibroblastgrowthfactor(FGF)-2withbeta-tricalciumphosphate( β -TCP)onthebeagledog1-wallperiodontaldefectmodel.Biochem.Biophys.Res.Commun,403:345-350,2010SMurakami,SYamada,TNozaki,M.Kitamura13.FGF-2stimulatesperiodontaltissueregenerationClinicalAdvancesinPeriodontics1:95-99,2011M.TakedachiHOohara,BJSmith,MIyama,MKobashi,14.KMaeda,CLLong,MBHumpherey,BJStoecker,SToyosawa,LFThompson,SMurakami.CD73-generatedadenosinepromotesosteoblastdifferentiationJ.Cell.Physiol.227:2622-2631,2012.
Shinya Murakami
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Clinical Application of Er:YAG Laser in Periodontal and Peri-implant Therapy
Yuichi Izumi D.D.S., Ph.D.ProfessorandChair,DepartmentofPeriodontology,GraduateSchoolofMedicalandDentalSciences,TokyoMedicalandDentalUniversity
Recently,variouslasershavebeenusedforawiderangeoforalconditions.Lasershavenumerousadvantageousphysicalpropertiessuchasablation,hemostasis,bactericidaleffectaswellasphoto-bio-modulation,makingitsuitablefortreatinginflammatoryandinfectiousconditions.Thus,theuseoflasersisconsideredsafeandeffectivefortreatingoraldiseasessuchasperiodontitisandperi-implantitis.
Amongvariouslasersystems,inparticular,therecentdevelopmentoftheEr:YAGlaserhasexpandedtheapplicationoflaserstohardtissuetreatmentinperiodonticssincethe2.94µmwavelengthcanbeappliedonbothdentalsoftandhardtissues.TheEr:YAGlaserhasvariouscharacteristicsadvantageousforsoftandhardtissuemanagement,suchaseasyandpreciseablation,cutting,debridementandrecontouringwithminimalthermaldamagetothesurroundingtissuesunderwaterirrigation.Nowadays,theEr:YAGlaseriseffectivelybeingusedforvarioustissuemanagementssuchasgingivaltissueablation,dentalrootandimplantfixturesurfacedebridement,andosseoussurgery.
Inperiodontalandperi-implantapplications,theEr:YAGlaseriscapableoftreatingalveolarbonetissueeitherdirectlyorindirectlyduringosseousmanagement.AnimalstudiesreportedfasterwoundhealinginbonetissuefollowingEr:YAGlaserablationcomparedtoburcutting,aswellassignificantlyenhancedandfavorablenewboneformationfollowingsurgicaltreatmentofperiodontitisorperi-implantitisusinganEr:YAGlasercomparedtomechanicalinstrumentation.ThesepositiveresultsareconsideredtobeduetothehighdecontaminationanddetoxificationeffectsoftheEr:YAGlaseronthediseasedsite,thepronouncedbleedingfromthelaser-treatedbonesurface,aswellastheproductionofcharacteristicmicrostructuresontheablatedboneandrootsurfaces,whichpossiblyenhancesfibrinandbloodclotretention.Inaddition,potentialphoto-bio-modulationeffectsoflow-levellasereffectwhichinducesavarietyofbiologicalresponsesmaybepartlyinvolvedintheincreasedboneformation.Giventheseobservationsandevidences,theEr:YAGlaserhasbeenrecentlyestablishedasoneofthemostpromisinglasersystemsforperiodontalandperi-implanttherapy.
Inthispresentation,thecurrentclinicalapplicationsofEr:YAGlaserforvarioustreatmentssuchassofttissuemanagement,non-surgicalandsurgicalperiodontaltreatment,osseoussurgeryandperi-implanttherapywillbediscussed,basedonscientificevidencefromcurrentlyavailablebasicandclinicalstudiesaswellasclinicalcases.
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Yuichi Izumi
CURRICULUM VITAE
Education
1979 D.D.S.degree:TokyoMedicalandDentalUniversity,FacultyofDentistry
1983 Ph.D.degree:TokyoMedicalandDentalUniversity,GraduateSchool,PeriodontologyinDentalResearchDivision
1983 ClinicalSpecialtyPrograminPeriodontology,TokyoMedicalandDentalUniversity
Position
1983-1992 Instructor,DepartmentofPeriodontology,Tokyo MedicalandDentalUniversity1987-1989 MaîtreAssistant,DepartmentofOral PhysiopathologyandPeriodontology,University ofGeneva1992-1999 AssociateProfessor,Departmentof Periodontology,KagoshimaUniversityDental School1999-2003 ProfessorandChair,Departmentof Periodontology,KagoshimaUniversityDental School2003-2007 ProfessorandChair,Departmentof Periodontology,FieldofOralandMaxillofacial Rehabilitation,CourseforDevelopmental Therapeutics,KagoshimaUniversityGraduate SchoolofMedicalandDentalSciences VicePresident,KagoshimaUniversityMedical andDentalHospital2007-Present ProfessorandChair,Departmentof Periodontology,GraduateSchoolofMedicaland DentalSciences,TokyoMedicalandDental University2008-Present AssistantDirector,TokyoMedicalandDental UniversityHospitalofDentistry
Award and Honors
REarlRobinsonPeriodontalRegenerationAward,1.AmericanAcademyofPeriodontology.(2009.9.15.Boston)WilliamJ.GiesAwardClinicalResearchCategory,2.InternationalAssociationofDentalResearch/AmericanAssociationofDentalResearch.(2012.6.20.IguaçuFalls,Brazil
Publications
1. TanakaK,IwasakiK,FegaliK,KomakiM,IshikawaI,IzumiY.Comparisonofcharacteristicsofperiodontalligamentcellsobtainedfromoutgrowthandenzyme-digestedculturemethods.ArchOralBiol,56:380-388,2011.
2. EbeN,Hara-YokoyamaM,IwasakiK,IsekiS,OkuharaS,Podyma-InoueK,TerasawaK,WatanabeA,AkizukiT,WatanabeH,YanagishitaM,IzumiY.PocketepitheliuminthepathologicalsettingforHMGB1release.JDentRes,90(2):235-240,2011.
3. AoyamaN,SuzukiJ,WangD,OgawaM,KobayashiN,HanataniT,TakeuchiY,IzumiY,IsobeM.Porphyromonasgingivalispromotesmurineabdominalaorticaneurysmsviamatrix metalloproteinase-2induction.JPeriodontalRes,46(2):176-183,2011.
4. IzumiY,AokiA,YamadaY,KobayashiH,IwataT,AkizukiT,SudaT,NakamuraS,Wara-AswapatiN,UedaM,IshikawaI.Currentandfutureperiodontaltissueengineering.Periodontol2000,56:166-187,2011.
5. CKobayashi,KYaegaki,BCalenic,NIshkitiev,TImai,HIi,IAoyama,HKobayashi,YIzumi,MHaapasalo.HydrogenSulfideCausesApoptosisinHumanPulpStemCells.JEndod,37:479–484,2011.
6. M.Kitamura,M.Akamatsu,M.Machigashira,Y.Hara,R.Sakagami,T.Hirofuji,T.Hamachi,K.Maeda,M.Yokota,J.Kido,T.Nagata,H.Kurihara,S.Takashiba,T.Sibutani,M.Fukuda,T.Noguchi,K.Yamazaki,H.Yoshie,K.Ioroi,T.Arai,T.Nakagawa,K.Ito,S.Oda,Y.Izumi,Y.Ogata,S.Yamada,H.Shimauchi,K.Kunimatsu,M.Kawanami,T.Fujii,Y.Furuichi,T.Furuuchi,T.Sasano,E.Imai,M.Omae,S.Yamada,M.Watanuki,andS.Murakami,FGF-2stimulatesperiodontalregeneration:Resultsofamulti-centerrandomizedclinicaltrial.JDentRes,90:35-40,2011.
6. TakahashiM,ChenZ,WatanabeK,KobayashiH,NakajimaT,KimuraA,IzumiY.Toll-likereceptor2genepolymorphismsassociatedwithaggressiveperiodontitisinJapanese.OpenDentJ,5:190-194,2011.
7. KatagiriS,NittaH,NagasawaT,IzumiY,KanazawaM,MatsuoA,ChibaH,MiyazakiS,MiyauchiT,NakamuraN,OsekoF,KanamuraN,AndoY,HanadaN,InoueS.Reducedmasticatoryfunctioninnon-elderlyobeseJapaneseadults.ObesResClinPract,5(4):e279-286,2011.
8. AkiyamaF,AokiA,Miura-UchiyamaM,SasakiKM,IchinoseS,UmedaM,IshikawaI,IzumiY.Invitrostudiesoftheablationmechanismofperiodontopathicbacteriaanddecontaminationeffectonperiodontallydiseasedrootsurfacesbyerbium:yttrium-aluminum-garnetlaser.LasersMedSci,26(2):193-204,2011.Erratumin:LasersMedSci26(2):277,2011.
9. WangH,WatanabeH,OgitaM,IchinoseS,IzumiY.Effectofhumanbeta-defensin-3ontheproliferationoffibroblastsonperiodontallyinvolvedrootsurfaces.Peptides32:888-894,2011.
10.TsumanumaY,IwataT,WashioK,YoshidaT,Yamada
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A,TakagiR,OhnoT,LinK,YamatoM,IshikawaI,OkanoT,IzumiY.Comparisonofdifferenttissue-derivedstemcellsheetsforperiodontalregenerationinacanine1-walldefectmodel.Biomaterials,32:5819-5825,2011.
11.ToyofukuT,InoueY,KuriharaN,KudoT,JibikiM,SuganoN,UmedaM,IzumiY.Differentialdetectionrateofperiodontopathicbacteriainatherosclerosis.SurgToday41:1395-1400,2011.
12. IshihataK,WakabayashiN,WadachiJ,AkizukiT,IzumiY,TakakudaK,IgarashiY.ReproducibilityofPocketDepthMeasurementbyExperimentalPeriodontalProbeIncorporatingOpticalFiberSensor.JPeriodontol,83:222-227,2012.
13.KomakiM,IwasakiK,ArzateH,NarayananAS,IzumiY,MoritaY.CementumQ1pro t ein1 (CEMP1)inducesacementoblasticphenotypeandreducesosteoblasticdifferentiationinperiodontalligamentcells. JCellPhysiol,227:649-657,2012.
14.KobayashiN,SuzukiJ,OgawaM,AoyamaN,HanataniT,HirataT,NagaiR,IzumiY,IsobeM.Porphyromonasgingivalisacceleratesneointimalformationafterarterialinjury.JVascRes,49:417-424,2012.
15.TakeuchiY,NagasawaT,KatagiriS,KitagawaraS,KobayashiH,KoyanagiT,IzumiY.Salivarylevelsofantibacterialpeptide(LL-37/hCAP-18)andcotinineinperiodontitispatients.JPeriodontol,83:766-772,2012.
16.AoyamaN,SuzukiJ,OgawaM,WatanabeR,KobayashiN,HanataniT,YoshidaA,AshigakiN,IzumiY,IsobeM.Clarithromycinsuppressestheperiodontalbacteria-acceleratedabdominalaorticaneurysmsinmice.JPeriodontalRes,47:463-469,2012..
17.Himeno-AndoA,IzumiY,YamaguchiA,IimuraT.Structuraldifferencesintheosteocytenetworkbetweenthecalvariaandlongbonerevealedbythree-dimensionalfluorescencemorphometry,possiblyreflectingdistinctmechano-adaptationsandsensitivities.BiochemBiophysResCommun,417:765-770,2012.
18.HayakumoS,ArakawaS,ManoY,IzumiY.Clinicalandmicrobiologicaleffectsofozonenano-bubblewaterirrigationsasanadjuncttomechanicalsubgingivaldebridementinperiodontitispatientsinarandomizedcontrolledtrial.ClinOralInvest,inpress
19. SekinishiA,SuzukiJ,AoyamaN,OgawaM,WatanabeR,KobayashiN,HanataniT,AshigakiN,HirataY,NagaiR,IzumiY,IsobeM.AperiodontalpathogenAggregatibacteractinomycetemcomitansdeterioratespressureoverload-inducedmyocardialhypertrophyinmice.IntHeartJ,53:324-330,2012.
20.HanataniT,SuzukiJ,OgawaM,AoyamaN,KobayashiN,HirataY,NagaiR,IzumiY,IsobeM.TheperiodontalpathogenAggregatibacteractinomycetemcomitansdeterioratesventricularremodelingaftermyocardialinfarctioninmice.IntHeartJ,53:253-256,2012.
21.BhartiP,KatagiriS,NittaH,NagasawaT,KobayashiH,
Yuichi Izumi
TakeuchiY,IzumiyamaH,UchiyamaI,InoueS,IzumiY.Periodontaltreatmentwithtopicalantibioticsimprovesglycemiccontrolinassociationwithelevatedserumadiponectininpatientswithtype2diabetesmellitus.ObesResClinPract,inpress.
22.KatagiriS,NagasawaT,KobayashiH,TakamatsuH,BhartiP,IzumiyamaH,UchimuraI,TagamiT,SuzukiT,NanbaraH,TaniguchiY,HayakumoS,KoyanagiT,Himeno-AndoA,GotoMakiKajioH,TakahashiY,IzumiY,NodaM.Improvementofglycemiccontrolafterperiodontaltreatmentbyresolvinggingivalinflammationintype2diabeticpatientswithperiodontaldisease.JDiabetesInvest,3:402-409,2012.
23.HosomiN,AokiS,MatsuoK,DeguchiK,MasugataH,MuraoK,IchiharaN,OhyamaH,DobashiH,NezuT,OhtsukiT,YasudaO,SoejimaH,OgawaH,IzumiY,KohnoM,TanakaJ,MatsumotoM.AssociationofSerumAnti-PeriodontalPathogenAntibodywithIschemicStroke.CerebrovascDis,34:385-392,2012.
24.NanbaraH,Wara-aswapatiN,NagasawaT,YoshidaY,YashiroR,BandoY,KobayashiH,KhongcharoensukJ,HormdeeD,PitiphatW,BochJA,IzumiY.ModulationofWnt5aexpressionbyperiodontopathicbacteria.ProsONEinpress
25.AlmehdiA,AokiA,IchinoseS,TaniguchiY,SasakiKM,EjiriK,SawabeM,ChuiC,KatagiriS,IzumiY.HistologicalandSEManalysisofrootcementumfollowingirradiationwithEr:YAGandCO2lasers.LasersMedSci,inpress
26.TaniguchiY,AokiA,MizutaniK,TakeuchiY,IchinoseS,TakasakiAA,SchwarzF,IzumiY.OptimalEr:YAGlaserirradiationparametersfordebridementofmicrostructuredfixturesurfacesoftitaniumdentalimplants.LasersMedSci,inpress
27.ChuiC,HiratsukaK,AokiA,TakeuchiY,AbikoY,IzumiY.BlueLEDinhibitsthegrowthofPorphyromonasgingivalisbysuppressingtheexpressionofgenesassociatedwithDNAreplicationandcelldivision.LaserSurgMed,inpress
28. IwasakiK,KomakiM,YokoyamaN,TanakaY,TakiA,KimuraY,TakedaM,OdaS,IzumiY,MoritaI.PeriodontalLigamentStemCellsPossesstheCharacteristicsofPericytes.JPeriodontol,inpress
29.YamadaY,NakamuraS,ItoK,UmemuraE,HaraK,NagasakaT,AbeA,BabaS,FuruichiY,IzumiY,KleinOD,WakabayashiT.InjectableBoneTissueEngineeringusingExpandedMesenchymalStemCells.StemCells,inpress
30.AshigakiN,SuzukiJ,OgawaM,WatanabeR,AoyamaN,KobayashiN,HanataniT,SekinishiA,ZempoH,TadaY,TakamuraC,WakayamaK,HirataY,NagaiR,IzumiY,IsobeM.PeriodontalBacteriaAggravateExperimentalAutoimmuneMyocarditisinMice.AmJPhysiolHeartCircPhysiol,inpress
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M E M O
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P o s t e r S e s s i o n
Sixth Retreat 2013
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Poster Session List
Name Grade
SmritiAryalA.C. 4
XuRen 4
ZhouMengyu 4
ChuiChanthoeun 4
SamirKumarPal 4
MDABDULLAALMASUDKHAN 4
ChihoWatanabe 4
YasuyukiShimada 4
YukiMatsushita 4
AzusaYamada 4
KoyoTakimoto 4
TomokiMuramatsu 4
PrasansuttipornTaweesak 4
SahaniMayurbhaiHimatbhai 4
Bakhsh,TurkiAbdulsalamA. 3
MarwaMadi 3
MaChengshan 3
YukiYamaguchi 3
BijayaHaobam 3
ZayarLin 3
RumanaKhanom 3
DuartePuertoCarolinaLizeth 3
YeChangChang 3
DawudAbduweli 3
ChengXu 3
YujiTsuchido 3
NorihikoAshigaki 3
JunpeiShirakawa 3
Md.SofiqulIslam 3
MasakoYoshizaki 3
YukihikoHashida 3
ThitthaweeratSuppason 3
HisanoriHasegawa 3
ShinFukuda 3
TadashiHosoya 3
NaokiKimura 3
44
M E M O
45
Name Grade
MasayoshiUezono 3
Mandurah,MonaMohammadM. 3
MayumiOgita 3
WaruneePluemsakunthai 3
NadilaWali 3
GerardoJoseJovesMendez 3
NurmaaDashzeveg 2
SuphanantachatSupreda 2
RajapaksheMudiyanselageAnupamaRasadariRajapakshe 2
KahaerAbula 2
TakehitoOno 2
GuJie 2
LeePhenix 2
SurapornsawasdThunyaporn 2
ThanitPrasitsak 2
AlaaAbdulahadTurkistani 2
LodhaEna 2
ThanatvarakornOrnnicha 2
WakaYokoyama 2
YusukeMatsuo 2
YokoNakazato 2
MaheswariKuppusamy 2
MohannadIssaMichaelNassar 2
KenjiOgura 2
TakayukiYamada 2
UeharaDanielaTiaki 1
Nuylan,MichelleLoyola 1
MakiriKawasaki 1
HuaQiao 1
Khunkar,SaharJameelM 1
NatsukaUmezawa 1
TetsuyaSaito 1
KenchiTakenaka 1
Alsayed,EhabZakiE 1
KongKalyan 1
BabaBista 1
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M E M O
47
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M E M O
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