Dr. Myrto - Panagiota Zacharof Supervisor: Dr.R.W.Lovitt

Dr. Myrto-Panagiota Zacharof

Supervisor: Dr.R.W.Lovitt

The Production Of Bacteriocins From

Lactic Acid Bacteria

3L.plantarum

L.casei

L.lactis

• Media Optimisation

• Extraction strategy

• Chemical nature

Protein assay

trypsination

Sizing

• Quantification Assay

y = -0.0019x + 0.3453R2 = 0.9758

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0 50 100 150 200Nisin IU/ml

μ m

ax (h

-1)

μ max (h-1) of L.delbruckii under a

range of Nisin Concentrations

between 0-200 IU/mLinear fitting is used

• Concentration &Purification Method

• Self Life

ResultsThe 3 strains are indeed producing bacteriocins

• Chemical Nature

Results

0

10

20

30

40

50

1 10 100 1000 10000

Volume (%

)

Size (d.nm)

Statistics Graph (8 measurements)

Mean w ith Max-Min error bar

• Bacteriocins Activity

Results

Nisin &Bacteriocins

Target strains

• Bacteriocins Potency

Results

Bacteriocins

Target Strain

• Self LifeResults

The Finishing Line

• Further Developments

The Finishing Line

Acknowledgments

Dr.R.W.Lovitt Dr.M.Jones Dr.P.Williams Dr.I.Jung

• Any questions?

• Email:M.Zacharof@swansea.ac.uk

Thankyou!!