Configuring ACQUITY UPLC I-Class System Components715003144 REV.C 3 OF 11 CONFIGURING ACQUITY UPLC I-CLASS SYSTEM COMPONENTS REQUIREMENTS: • The mass spectrometer is a destructive
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715003144 REV. C 1 OF 11
Configuring ACQUITY UPLC I-Class System Components
This document outlines recommended configurations for ACQUITY UltraPerformance LC® (UPLC) I-Class System components. It includes information on:
• Core system components• LC detectors used with I-Class, page 2• Mass spectrometers used with I-Class, page 2• Recommended configurations, page 3• Configuration diagrams, page 5
Core system componentsThe ACQUITY UPLC I-Class core system consists of the following components (see Figure 1):
• Binary solvent manager• Sample manager • Column heater-active (CH-A) or optional column manager-active (CM-A)• UPLC detector (TUV, PDA, ELS, FLR, SQ, or TQ)• Solvent tray
The above components assemble in a single or double stack, with the optional Common Platform Sample Organizer (CPSO) positioned to the left of the ACQUITY UPLC sample manager (Figure 1).
NOTE: Use the flex cart only with the core ACQUITY UPLC system (with or without the Common Platform Sample Organizer, or CPSO).
Figure 1 - ACQUITY UPLC I-Class System components
Booster = Spacer for SQ/TQBSM = Binary Solvent ManagerCH-A = Column Heater-ActiveCH-A Extended = CH-A Extention Arm KitCH-30A = 30cm Column Heater/Cooler
CM-A = Column Manager-ActiveCM-Left = CM-A installed as left inletCM-A Right = CM-A installed as right inletCPSO = Common Platform Sample Organizer
ELS = Evaporative Light-Scattering DetectorFLR = Fluorescence DetectorPDA = Photodiode Array DetectorSM-FL = Sample Manager Fixed Loop
SM-FTN = Sample Manager Flow-through NeedleSQ/TQ = Single Quadrupole or Tandem QuadrupoleTray = Solvent TrayTUV = Tunable Ultraviolet Detector
TUV/PDA
ELS/FLR
CM-A
BoosterMicroTee
CPSO
Core System Additional Components
SYNAPT G2
Tray
UPLC Detector
CH-A
BSM
Xevo TQ-S
XEVO TQ/TQ-S
Glossary
CH-A Extended
Mass detectorsLC detectors
CH-30A
SMFLEX CART
SQ/TQ
Multi-detector drip tray
XEVO TQD/SQD 2
© 2012 WATERS CORPORATION. ALL RIGHTS RESERVED.
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LC detectors used with I-ClassThe term LC detector refers to the following Waters instruments:
• Tunable Ultraviolet (TUV) Detector • Photodiode Array (PDA) or PDA Extended Wavelength (eλ) Detector • Evaporative Light-Scattering (ELS) Detector• Fluorescence (FLR) Detector
You can include a single LC detector (TUV, PDA, PDAeλ, ELS, or FLR) in the core system stack or with additional UPLC detectors in a second stack of components. You can also place UPLC detectors on top of an SQD or TQD, with or without the optional booster.
NOTE: Use of the ELS and FLR detectors is less than optimal.
CAUTION: USE OF ANY STACKING CONFIGURATION OTHER THAN THE CORE SYSTEM WILL INCREASE DISPERSION.
REQUIREMENTS:
• The ELS Detector is a destructive detector and must be the last component in a flow path or detector series.
WARNING:TO AVOID EXPOSURE TO SOLVENT VAPORS, PLACE THE ELS DETECTOR IN AN APPROPRIATE POSITION TO ACCOMMODATE ITS EXHAUST REQUIREMENTS. DO NOT ALLOW DETECTOR EXHAUST TO ENTER THE LABORATORY ATMOSPHERE.
• Because the flow cell of the FLR detector is pressure-rated to a maximum of 3447 kPa (34 bar, 500 psi), the FLR Detector must be the last component in a flow path or detector series.
• The backpressure on the flow cells of the TUV and PDA detectors must not exceed 6895 kPa (69 bar, 1000 psi).
• To minimize dispersion and backpressure between instrument components, use the shortest length of tubing possible to connect detector flow cells.
Mass spectrometers used with I-ClassThe ACQUITY UPLC I-Class System can be configured with all currently supported mass spectrometry (MS) systems. This guide includes recommendations for only SQ/TQ, Xevo tandem quadrupole, and SYNAPT G2 mass spectrometers:
Table 1: Configuration recommendations
MS Model Position Stack allowances
Single Quadrupole (SQ) Detec-tor
Right of core system 2 instrumentsa on top
a. Instrument = LC detector (TUV, PDA, ELS, FLR) or column compartment (CM-A).
Tandem Quadrupole (TQ) Detector
Right of core system 2 instruments on top
Xevo TQ-S or TQ MS Right of core system 0 instruments on top
Xevo TQD or SQD 2 Left of core systemException: When sample organizer is used, right of core system
1 instrument on top
SYNAPT G2 Right of core system 0 instruments on top
CONFIGURING ACQUITY UPLC I-CLASS SYSTEM COMPONENTS
715003144 REV. C 3 OF 11
REQUIREMENTS:• The mass spectrometer is a destructive detector and must be the last component in a
flow path or detector series.• Minimize the length of tubing used to connect detector flow cells to the inlets of the SQ
and TQ detectors. The relatively high backpressure of eluent flow through long lengths of small-ID tubing can damage the flow cells.
• In the left-stack configuration, leave a minimum of 20 mm between the Xevo TQD/SQD 2 and the ACQUITY UPLC System.
Recommended configurationsThe configuration diagrams in Figures 2 through 5 show the placement Waters recommends for each component of the ACQUITY UPLC I-Class system:
• Recommended configurations for I-Class systems with single LC detector, page 5• Recommended configurations for I-Class systems with single MS detector, page 6• Recommended configurations for I-Class systems with dual LC detectors, page 7• Recommended configurations for I-Class systems with dual LC/MS detectors, page 8• Recommended configurations for I-Class systems with OA triple detector (1), page 9• Recommended configurations for I-Class systems with OA triple detector (2), page 10• Recommended configurations for I-Class systems with SYNAPT G2, page 11
On the multiple-detector configuration diagrams, the recommended post-column flow path is also shown. These systems may require a flow splitter (MicroTee), MS booster, or multi-detector drip tray. When coupling a core single-stack system with a mass spectrometer such as a SYNAPT, use a flex cart.
Additional parts neededCertain stacking configurations may require the use of additional parts that are not included with the system.
When to use a MicroTeeSplit the eluent flow using a MicroTee when:
• The system configuration includes two destructive detectors, such as the ELS, TQ detector, or SQ detector.
• The system configuration includes an FLR detector with a destructive detector.• You want to increase MS sensitivity in a system by restricting the flow rate range.
NOTE: Consult the appropriate user documentation for detector flow rate requirements.
CAUTION: USE OF THE MICROTEE REQUIRES LARGER-ID TUBING, WHICH WILL INCREASE DIS-PERSION.
Figures 3 through 5 show configurations requiring a MicroTee.
NOTE: For more information on splitting the eluent flow, see Designing a Split Flow Sys-tem with a MicroTee (PN 715001704).
When to use an MS boosterPlace an MS booster (or spacer) on top of the SQ or TQ detector when the column heater (CH-A) extension arm is positioned over the SQ/TQ. Place the UPLC detector on top of the MS booster in the specified configurations (Figures 2, 3, and 5).
NOTE: Only the SQ and TQ detectors need a booster.
CONFIGURING ACQUITY UPLC I-CLASS SYSTEM COMPONENTS
715003144 REV. C 4 OF 11
When to use a Flex CartUse a flex cart to adjust the height of the core UPLC system (with or without CPSO) when it is configured in a single stack (Figure 3). This mobile system will be easier to locate as close as possible to the inlet of a mass spectrometer such as a SYNAPT system.
When to use a multi-detector drip trayIf the system has more than one detector - for example, a UV detector on top of an ELS detector - you must install the multi-detector drip tray (205000355). (See Figures 3, 4, and 5.)
When to use a swivel drain kitUse a swivel drain kit (205000499) when placing a CM-A on top of a bench or detector.
Configuring the column compartments
CM-A as a right or left inletThe CM-A can accommodate both left and right inlet configurations in order to minimize dispersion when interfacing with MS.
• Configure the CM-A as a right inlet when the CM-A is positioned above the core stack.• Configure CM-A as left inlet when the CM-A is positioned:
- in a second stack to the right of the core stack, or - above the core stack in configurations that use an MS and sample organizer
NOTE: When configuring the CM-A as a left inlet in the core stack, use a 22.5” tube (700005480) between the injection valve and left switching valve. Replace the low pressure fittings with high pressure gold compression screws and two-piece fer-rules.
CH-A ExtendedThe CH-A in the extended position brings the column outlet closer to the inlet of the source probe, thus reducing dispersion. The vertical shift adapter is primarily used in third-party MS to raise or lower the arm (Figure 2). When configuring with a CH-A vertical shift adapter, use the 18.5” APH (205000774) to ensure proper routing to the injection valve.
Figure 2 - CH-A in extended position
Configuring with a CPSOYou can use a CPSO anytime there is nothing to the left of the core system. Always position it to the left of the sample manager.
Verticalshiftadapter
Arm
CONFIGURING ACQUITY UPLC I-CLASS SYSTEM COMPONENTS
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CO
NFIG
UR
ING A
CQ
UITY U
PLC I-C
LASS S
YSTEM
CO
MPO
NEN
TS
LC detector
Double stack
Tray
BSM
TUV/PDA*ELS/FLR
SM CM-ALeft
Configuration diagrams
Figure 3 - Recommended configurations for I-Class systems with single
Single LC DetectorSingle stack
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Use the Flex Cart only with the core I-Class system in a single stack.- Use of the ELS or FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- If placing a CM-A on top of a detector, use a swivel drain kit.
Tray
TUV/PDA*ELS/FLR
CH-A
BSM
CPSO
SM
FLEX CART
CPSOTray
CM-ARight
BSM
TUV/PDA*ELS/FLR
SM
FLEX CART
CPSO
Tray
BSM
CM-ARight
TUV/PDA/FLR/ELS*
SM
Tray
BSM
TUV/PDAFLR/ELS*
SM
CH-A
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PLC I-C
LASS S
YSTEM
CO
MPO
NEN
TS
S detector
k Configuration (no CPSO)
Tray
CM-ARight
BSM
SMXEVO TQD/SQD 2
20+ mm
Tray
CM-ARight
BSM
SMSQ/TQ
Figure 4 - Recommended configurations for I-Class systems with single M
Single MS Detector
Right-stack Configuration Left-stac
With Xevo
With SQ/TQ
Tray
BSM
CH-A Extended
CPSO
Tray
BSM
CPSO
CM-ALeft
SM
SM
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Use the Flex Cart only with the core I-Class system in a single stack.- Use of the ELS or FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- If placing a CM-A on a detector, use a swivel drain kit.- In the left stack configuration, leave a minimum of 20 mm between the XEVO TQD/SQD 2 and an ACQUITY UPLC system.
Tray
CH-A
BSM
SM
XEVO TQD/SQD 2
20+ mm
Tray
BSM
CPSO
CM-ALeft
SMSQ/TQ
Tray
CH-A
BSM
SM
SQ/TQSQ/TQ
Xevo TQ-S
XEVO TQ-S/TQ MS
Tray
BSM
CH-A Extended
CPSOSM
Xevo TQ-S
XEVO TQ-S/TQ MS
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CO
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CQ
UITY U
PLC I-C
LASS S
YSTEM
CO
MPO
NEN
TS
LC detectors
n)
A
R*
Multi-detectordrip tray
Figure 5 - Recommended configurations for I-Class systems with dual
Dual LC Detector (Right-Stack Configuratio
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Do not use the flex cart with these configurations.- *Use of the FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- If placing a CM-A on a detector, use a swivel drain kit.
BSM
CH-AExtended
TUV/PDA
ELS/FLR*
Multi-detectordrip tray
SM
BSM
TUV/PD
ELS/FL
CM-ALeft
SM
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CO
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CQ
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PLC I-C
LASS S
YSTEM
CO
MPO
NEN
TS
/MS detectors
guration)
ath (Requires MicroTee)
Booster (withSQ/TQ only) BSM
Tray
TUV/PDAELS/FLR*
CM-ALeft
lti-detector tray
SM
BSM
Tray
TUV/PDAELS/FLR*
SM
SQ/TQ
XEVO TQD/SQD 2
CM-ALeftQD 2
Figure 6 - Recommended configurations for I-Class systems with dual LC
Dual LC/MS Detector (Right-Stack Confi
With SQ/TQ
Series Flow Path Parallel Flow P
TUV/PDAFLR*
BSM
Booster (withSQ/TQ only)
Tray
BSM
Tray
SQ/TQ
Tray
BSM
CM-ALeft
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Do not use the flex cart with these configurations.- *Use of the FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- If placing a CM-A on a detector, use a swivel drain kit.- Use of a MicroTee accurately splits flow while minimizing dispersion as far as possible.
SQ/TQ SQ/TQ
TUV/PDA/FLR*
TUV/PDAELS/FLR*
CH-AExtended
CH-AExtended
Multi-detectordrip tray Multi-detector
drip tray
Mudrip
SM SM SM
TUV/PDAFLR*
BSM
Tray
Tray
BSM
CM-ARight
TUV/PDAFLR*
SM SM
BSM
Tray
TUV/PDAELS/FLR*
CH-AExtended
SM
CH-A
XEVO TQD/S
WithXevo TQ-SXevo TQ MS
Xevo TQ-S
XEVO TQ-S/TQ MS
Xevo TQ-S
XEVO TQ-S/TQ MS
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CQ
UITY U
PLC I-C
LASS S
YSTEM
CO
MPO
NEN
TS
le detector (1)
D/SQD2
CM-ARight
ELS/FLR*
TUV/PDA
Multi-detectordrip tray
SM
Tray
BSM
XEVO TQD/SQD 2
20+ mm
icroTee; do not use SO)
Figure 7 - Recommended configurations for I-Class systems with OA trip
Open Access Triple Detector with SQ/TQ or Xevo TQRight-hand configuration (requires MicroTee; can use SO)
Tray
BSM
CM-ALeft
Tray
CH-A
CH-A Extended
Tray
BSM
TUV/PDA
ELS/FLR*
Booster(withSQ/TQonly)
SQ/TQ SQ/TQ
TUV/PDA
Multi-detectordrip tray
Multi-detectordrip tray
ELS/FLR*
ELS/FLR*
Multi-detectordrip tray
TUV/PDA
SM SM
SM
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Do not use the flex cart with these configurations.- *Use of the ELS or FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- Use of a MicroTee accurately splits flow while minimizing dispersion as far as possible.- In the left stack configuration, leave a minimum of 20 mm between the XEVO TQD/SQD 2 and an ACQUITY UPLC system.- MassLynx support only.
Tray
CM-ALeft
ELS/FLR*
SM
BSMBSM
XEVO TQD/SQD 2
20+ mm
TUV/PDAELS/FLR*
XEVO TQD/SQD 2
BSM
Tray
TUV/PDA
SM
XEVO TQD/SQD 2
ELS/FLR*
CH-A Extended
Left-hand configuration (requires M
SQ/TQ
XEVOTQDSQD2
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LASS S
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CO
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le detector (2)
S
Xevo TQ-S/TQ MS
ctor
Figure 8 - Recommended configurations for I-Class systems with OA trip
Open Access Triple Detector with Xevo TQ-S/TQ M
Right-hand configuration (requires MicroTee; can use SO)
NOTES: - Arrows indicate the general flow path through the ACQUITY UPLC I-Class System after the sample manager. - Do not use the flex cart with these configurations.- *Use of the ELS or FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.- If placing a CM-A on a detector, use a swivel drain kit.- Use of a MicroTee accurately splits flow while minimizing dispersion as far as possible.- MassLynx support only.
BSM
TUV/PDA
ELS/FLR*
CM-ALeft
SM
BSM
CH-AExtended
TUV/PDA
ELS/FLR*
SM
Xevo TQ-S/TQ MS
Multi-detectordrip trays
Multi-detedrip trays
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NAPT G2
PT G2
PT G2
Figure 9 - Recommended configurations for I-Class systems with SY
SYNAPT G2
NOTES:- Use the Flex Cart only with the core I-Class system in a single stack.- *Use of the ELS or FLR detector is less than optimal.- If using a CPSO, place it to the left of the sample manager.
Singledetector
Dual detector
Tray
TUV/PDA*ELS/FLR
CH-A
BSM
CPSO
SM
SYNAPT G2
FLEX CART
BSM
CPSO
Tray
CM-ARight
TUV/PDA*ELS/FLR
SM
SYNA
FLEX CART
Tray
BSM
CPSOSM
CM-ALeft
SYNA
FLEX CART
Tray
BSM
CPSOSM
SYNAPT G2
FLEX CART
CH-A
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