comparison of light and electron microscopes

Comparing Light and Electron Microscopes

Advantages of Electron Microscopes

• Greater magnification up to 1,000,000 x

• Greater depth of field

(With SEMs, 3-D )

• No complex lighting

• Greater resolution -1 nm

Disadvantages of Light Microscope

• Magnification only up to 1000-1,500 x

• Less depth of field

• May involve complex lighting requirements

• Less resolution - 1 micron

Light MicroscopesLight Microscopes

Light microscope images

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Microscopes ContinuedDisadvantages of

Electron Microscope• Expensive (1 million)• Expensive to Run• Image Black and

White• Dead Specimen• Requires Vacuum• Expert Preparation• Special Large Room• Affected by Magnetic

Fields

Advantages of Light Microscope

• Cheaper (100)• Cheaper to Run• Color image• Live or Dead

Specimen• No Vacuum Required• Simple Preparation• Small and Portable• Unaffected by

Magnetic Fields

Electron Microscope images

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SEMs TEMs

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435x (SEM)

40 x (SEM)

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1540 x

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3080 x

Eye of a Mosquito

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1760 x