Comparing Light and Electron Microscopes Advantages of Electron Microscopes • Greater magnification up to 1,000,000 x • Greater depth of field (With SEMs, 3-D ) • No complex lighting • Greater resolution -1 nm Disadvantages of Light Microscope • Magnification only up to 1000-1,500 x • Less depth of field • May involve complex lighting requirements • Less resolution - 1 micron
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Comparing Light and Electron Microscopes
Advantages of Electron Microscopes
• Greater magnification up to 1,000,000 x
• Greater depth of field
(With SEMs, 3-D )
• No complex lighting
• Greater resolution -1 nm
Disadvantages of Light Microscope
• Magnification only up to 1000-1,500 x
• Less depth of field
• May involve complex lighting requirements
• Less resolution - 1 micron
Light MicroscopesLight Microscopes
Light microscope images
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Microscopes ContinuedDisadvantages of
Electron Microscope• Expensive (1 million)• Expensive to Run• Image Black and
White• Dead Specimen• Requires Vacuum• Expert Preparation• Special Large Room• Affected by Magnetic
Fields
Advantages of Light Microscope
• Cheaper (100)• Cheaper to Run• Color image• Live or Dead
Specimen• No Vacuum Required• Simple Preparation• Small and Portable• Unaffected by
Magnetic Fields
Electron Microscope images
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