Transcript
BLOOD SMEAR EVAL
Quick scan on low power through entire slide for adequate distribution of cells
Check for plt clumping & atypical cells at feathered edge. (if there is plt clumping there is no need to try and count plt in the monolayer)
FEATHERED EDGE
Where the action is at: diff 40X morph 100X WBC morph, RBC morph, plt count (if no plt clumping at feathered edge)
MONOLAYER
Canine-Biconcave with area of central pallor 7umFeline-no consistent central pallor, vary more in shape 6um
NORMAL RBCS
Evidence of regeneration Polychromatophils-anisocytosis
nRBC (<4/100WBCs)
RBC MORPHOLOGY
AUTOAGGLUTINATION
Spherical, Loss normal bioconcave shape, no central pallor >5/HPF is abnormal
SPHEROCYTES
Often shrinking artifact-abn lytes, uremia, rattlesnake envenoation.
CRENATION/ECCHINOCYTES
Irregularly spaced membrane projections-abnormal accumulation of lipids in RBC membrane-metabolic, HAS, dz of liver, spleen and kidney
ACANTHOCYTES
Fragmented by mechanical injury. DIC, microvascular hemolysis, HAS, heartworm dz. 1/ 3HPF is abnormal
SCHISTOCYTE
Accumulations of damaged Hg-acetaminophen toxicosis, acute onion and zinc toxicity in dogs, DM, lymphoma in cats
HEINZ BODIES
Punctate aggregates of ribosomes-from highly regenerative anemia or lead toxicosis
BASOPHILIC STIPPLING
Nuclear remnants-regenerative anemia or splenic disease
HOWELL-JOLLY BODIES
# per 10HPF X 15,000
PLATELETS
Neutrophils-lobulated nucleus with pale to light pink/ purple cytoplasm
WBC MORPHOLOGY
U or J shaped nucleus, constriction of band <1/2 width of remainder, cytoplasm similar to mature neutrophils. <5% normal.
BAND NEUTROPHIL
Presence of dohle bodies, cytoplasmic basophilia (cytoplasm is more blud-purple), foamy cytoplasmic vacuolization (soap bubbles).
TOXIC CHANGES
Densely staining round to oval nuclei with small amounts of cytoplasm. Reactive (antigenic stimulation) darker cytoplasm, increased size.
LYMPHOCYTES
Lots of light to deep blue cytoplasm (darker then neut) with nuclei that is not round. Quite variable in appearance. Often larger then neuts and lymphs.
MONOCYTES
In low numbers, slightly smaller then neuts with less lobulated nuclei, contains prominent pink granules which are rod shaped in cats.
EOSINOPHILS
Largest, very uncommon, nucleus is ribbon like (not segmented) often with pink/ purple granules present.
BASOPHILS
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