B Cell Receptor Signaling · 2017. 10. 10. · #36438: IHC analysis of paraffin- embedded human tonsil using #36438. For Research Use only. Not For Use In Diagnostic Procedures. Subject

For pathway key and background please visit:

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B Cell Receptor Signaling

IP3R

IntracellularCa2+ Store

Ca2+

CREBMEF2C

p38

p38 JNK1/2

MKK3/4/6 MKK4/7

Lyn

Akt

PTEN

SHIP

Akt

FoxO

mTORGSK-3

IκBNFAT

p70S6K

LynSyk

Btk

SHP-2

PLCγ2

GabShc

GRB2

GR

B2

BLN

K

PKC

JNK Erk1/2

Erk1/2

MEK1/2

c-Raf

Ras

NF-κB

NF-κBIκB

IKK

PKC

CaMK

ATF-2 Jun Egr-1 Elk-1 Bcl-xL Bfl-1 NFAT

CD19

CD19

CD19

CD45

CaM

Bam32

CIN85

MEKKs

SOS

RasGAP

RasGRP

Bcl-6 Oct-2 Ets-1

CD40

LAB

LAB

CblCsk

Cbp/PAG

SHP-1BCAP

CARMA1

Ag

CD22 FcγRIIB1

FcγRIIB1

Bcl10

RhoA

Rap

Rac/cdc42

Rac

RapLRiam

Cofilin

Pyk2

HS1

Dok-1

Dok-3

CD19

PIR-B

Ezrin

Bam32

clathrin

TAK1

Calcineurin

CR

AC

Cha

nnel

Ora

i1

Ca2+

STIM1

SHP-2SHP-1

MALT1

GRB2 Vav

BCR

p85PI3Kp110

ProteasomalDegradation

ProteinSynthesis

Transcription

Growth Arrest,Apoptosis

DAGCa2+

Ca2+

PIP3

Transcription

mIgα/β α/β mIg

PI(4,5)P2

CytoskeletalRearrangements and

Integrin Activation

DAG

DAG

PIP3

IP3

LipidRaft

AggregationBCR

Internalization

Transmembrane Protein

Glucose Uptake

Glycolysis

ATPGeneration

Nucleus

Cytoplasm

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APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology

17IM

M14

7PW

H1E1

www.cellsignal.com/bcell

ANTIBODIES APPLICATIONS REACTIVITY

#15103 Aiolos (D1C1E) Rabbit mAb WB, IP, IHC-P, F, ChIP

H, M

#14895 BCL6 (D4I2V) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP

H

#4237 Bcl10 (C78F1) Rabbit mAb WB, IP H, M, R, (Mk)

#2764 Bcl-xL (54H6) Rabbit mAb WB, IP, IHC-P, IHC-F, IF-IC, F

H, M, R, Mk

#9115 Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb WB, IP, IF-IC H, M, (Mk)

#36438 BLNK (D3P2H) XP® Rabbit mAb WB, IP, IHC-P, F H, M

#3601 Phospho-BLNK (Tyr96) Antibody WB, IP H

#56044 Btk (D6T2C) Mouse mAb WB, IP, F H, M

#5082 Phospho-Btk (Tyr223) Antibody WB H, (M), (R)

#3574 CD19 Antibody WB, IP, IF-IC, F H, M

#3571 Phospho-CD19 (Tyr531) Antibody WB, IP H

#13917 CD45 (D9M8I) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F

H

#13333 CD79A (D1X5C) XP® Rabbit mAb WB, IHC-P, F H

#96024 CD79B (D7V2F) Rabbit mAb WB, IP, IHC-P H,M

#14732 Phospho-CD79A (Tyr182) (D1B9) Rabbit mAb IF-IC, F H, (M)

#5175 Cofilin (D3F9) XP® Rabbit mAb WB, IF-IC H, M, R, Mk, Dg

#3313 Phospho-Cofilin (Ser3) (77G2) Rabbit mAb WB, IF-IC H, M, R, Mk, B

#3145 Ezrin Antibody WB, IP, IHC-P, IF-IC, F

H, M, R, Mk, B

#3726 Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (48G2) Rabbit mAb

WB, IHC-P, IF-IC, E-P

H, M, R, Mk, (B)

#3890 HS1 (D83A8) XP® Rabbit mAb (Human Specific) WB, IP, IHC-P, IF-IC, F

H

#8714 Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb WB, IF-IC, F H, (M), (R)

#14859 Ikaros (D6N9Y) Rabbit mAb WB, IHC-P, IF-IC, F, ChIP, ChIP-seq

H, M

#9034 Ikaros (D10E5) Rabbit mAb WB, IF-IC, F, ChIP H

#15106 IRF-4 (D9P5H) Rabbit mAb WB, IP, ChIP H, M, R

#4299 IRF-4 (D43H10) Rabbit mAb WB, IP, ChIP H, R

#2796 Lyn (C13F9) Rabbit mAb WB, IP, IHC-P H, M, R, Mk

#2731 Phospho-Lyn (Tyr507) Antibody WB H, M, (R)

ANTIBODIES APPLICATIONS REACTIVITY

#8690 p38 MAPK (D13E1) XP® Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm, Mk, B, Pg, (C)

#9212 p38 MAPK Antibody WB, IHC-P, IF-IC, F H, M, R, Mk, GP, (C)

#4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb

WB, IP, IHC-P, IF-IC, F

H, M, R, Mk, Mi, Pg, Sc, (Hm), (C), (Z), (B), (Pg)

#9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb

WB, IF-IC, F H, M, R, Mk, Dm, Pg, Sc, (Hm), (Mi), (Z), (B)

#4631 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb

WB, IHC-P, IF-IC H, M, R, Mk, Dm, (Hm), (Mi), (Z), (Hr)

#9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb

WB, IP, IF-IC, F H, M, R, Mk, Sc, (Z)

#5861 NFAT1 (D43B1) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F

H, M

#3872 PLCγ2 Antibody WB, IP H, M, (R)

#3871 Phospho-PLCγ2 (Tyr1217) Antibody WB H, M, (R)

#9427 Phospho-c-Raf (Ser338) (56A6) Rabbit mAb WB H, M, R, Mk

#8955 Ras (D2C1) Rabbit mAb WB H, M, R, Mk

#3339 Ras (27H5) Rabbit mAb WB H, M, R, Mk, Dm

#2117 RhoA (67B9) Rabbit mAb WB H, M, R, Mk, B

#2727 SHIP1 (C40G9) Rabbit mAb WB, IP, IF-IC, F H

#2839 SHIP2 (C76A7) Rabbit mAb WB, IP, IF-IC, F H

#3759 SHP-1 (C14H6) Rabbit mAb WB H, M, (R)

#8849 Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb WB H, M

#3397 SHP-2 (D50F2) Rabbit mAb WB, IP H, M, R, Mk

#5431 Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb WB, IP, F M, R, (H)

#2109 Src (36D10) Rabbit mAb WB, IP, IHC-P, IF-F, IF-IC, F

H, M, R, Hm, Mk, B, Pg, (C)

#6943 Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb

WB, IP H, M, R, Mk

#2101 Phospho-Src Family (Tyr416) Antibody WB H, M, R, (C), (X)

#5668 STIM1 (D88E10) Rabbit mAb WB, IP, IF-IC H, M, R, Mk, B

#13198 Syk (D3Z1E) XP® Rabbit mAb WB, IP, IHC-P, F H, M, R

#2710 Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb WB, IP, IF-IC, F H, (M), (R)

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Key Products for B Cell Receptor SignalingVisit www.cellsignal.com/bcellproducts for full listing of antibodies, related kits, and reagents.

Serum-starved IgM-treated

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710: Confocal IF analysis of Ramos cells, serum-starved (overnight; left) or IgM-treated (12 ug/ml, 2 min; right), using #2710 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Syk (D3Z1E) XP® Rabbit mAb #13198: IHC analysis of paraffin-embedded human lymph node using #13198.

BLNK (D3P2H) XP® Rabbit mAb #36438: IHC analysis of paraffin- embedded human tonsil using #36438.

For Research Use only. Not For Use In Diagnostic Procedures.