For pathway key and background please visit: www.cellsignal.com/bcell B Cell Receptor Signaling IP 3 R Intracellular Ca 2+ Store Ca 2+ CREB MEF2C p38 p38 JNK1/2 MKK3/4/6 MKK4/7 Lyn Akt PTEN SHIP Akt FoxO mTOR GSK-3 IκB NFAT p70 S6K Lyn Syk Btk SHP-2 PLCγ2 Gab Shc GRB2 GRB2 BLNK PKC JNK Erk1/2 Erk1/2 MEK1/2 c-Raf Ras NF-κB NF-κB IκB IKK PKC CaMK ATF-2 Jun Egr-1 Elk-1 Bcl-xL Bfl-1 NFAT CD19 CD19 CD19 CD45 CaM Bam32 CIN85 MEKKs SOS Ras GAP Ras GRP Bcl-6 Oct-2 Ets-1 CD40 LAB LAB Cbl Csk Cbp/PAG SHP-1 BCAP CARMA1 Ag CD22 FcγRIIB1 FcγRIIB1 Bcl10 RhoA Rap Rac/ cdc42 Rac RapL Riam Cofilin Pyk2 HS1 Dok-1 Dok-3 CD19 PIR-B Ezrin Bam32 clathrin TAK1 Calcineurin CRAC Channel Orai1 Ca 2+ STIM1 SHP-2 SHP-1 MALT1 GRB2 Vav BCR p85 PI3K p110 Proteasomal Degradation Protein Synthesis Transcription Growth Arrest, Apoptosis DAG Ca 2+ Ca 2+ PIP 3 Transcription mIg α/βα/β mIg PI(4,5)P 2 Cytoskeletal Rearrangements and Integrin Activation DAG DAG PIP 3 IP 3 Lipid Raft Aggregation BCR Internalization Transmembrane Protein Glucose Uptake Glycolysis ATP Generation Nucleus Cytoplasm
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B Cell Receptor Signaling · 2017. 10. 10. · #36438: IHC analysis of paraffin- embedded human tonsil using #36438. For Research Use only. Not For Use In Diagnostic Procedures. Subject
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For pathway key and background please visit:
www.cellsignal.com/bcell
B Cell Receptor Signaling
IP3R
IntracellularCa2+ Store
Ca2+
CREBMEF2C
p38
p38 JNK1/2
MKK3/4/6 MKK4/7
Lyn
Akt
PTEN
SHIP
Akt
FoxO
mTORGSK-3
IκBNFAT
p70S6K
LynSyk
Btk
SHP-2
PLCγ2
GabShc
GRB2
GR
B2
BLN
K
PKC
JNK Erk1/2
Erk1/2
MEK1/2
c-Raf
Ras
NF-κB
NF-κBIκB
IKK
PKC
CaMK
ATF-2 Jun Egr-1 Elk-1 Bcl-xL Bfl-1 NFAT
CD19
CD19
CD19
CD45
CaM
Bam32
CIN85
MEKKs
SOS
RasGAP
RasGRP
Bcl-6 Oct-2 Ets-1
CD40
LAB
LAB
CblCsk
Cbp/PAG
SHP-1BCAP
CARMA1
Ag
CD22 FcγRIIB1
FcγRIIB1
Bcl10
RhoA
Rap
Rac/cdc42
Rac
RapLRiam
Cofilin
Pyk2
HS1
Dok-1
Dok-3
CD19
PIR-B
Ezrin
Bam32
clathrin
TAK1
Calcineurin
CR
AC
Cha
nnel
Ora
i1
Ca2+
STIM1
SHP-2SHP-1
MALT1
GRB2 Vav
BCR
p85PI3Kp110
ProteasomalDegradation
ProteinSynthesis
Transcription
Growth Arrest,Apoptosis
DAGCa2+
Ca2+
PIP3
Transcription
mIgα/β α/β mIg
PI(4,5)P2
CytoskeletalRearrangements and
Integrin Activation
DAG
DAG
PIP3
IP3
LipidRaft
AggregationBCR
Internalization
Transmembrane Protein
Glucose Uptake
Glycolysis
ATPGeneration
Nucleus
Cytoplasm
www.cellsignal.com/cellbiologyFor Research Use Only. Not For Use In Diagnostic Procedures.
www.cellsignal.com/cellbiologyFor pathway key and background please visit:
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology
17IM
M14
7PW
H1E1
www.cellsignal.com/bcell
ANTIBODIES APPLICATIONS REACTIVITY
#15103 Aiolos (D1C1E) Rabbit mAb WB, IP, IHC-P, F, ChIP
Key Products for B Cell Receptor SignalingVisit www.cellsignal.com/bcellproducts for full listing of antibodies, related kits, and reagents.
Serum-starved IgM-treated
Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710: Confocal IF analysis of Ramos cells, serum-starved (overnight; left) or IgM-treated (12 ug/ml, 2 min; right), using #2710 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Syk (D3Z1E) XP® Rabbit mAb #13198: IHC analysis of paraffin-embedded human lymph node using #13198.
BLNK (D3P2H) XP® Rabbit mAb #36438: IHC analysis of paraffin- embedded human tonsil using #36438.
For Research Use only. Not For Use In Diagnostic Procedures.