Ampliqon PCR Enzymes for DNA Amplification
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Ampliqon PCR Enzymes for DNA Amplification
Ampliqon page 2
CUSTOM-MADEAmpliqon was founded as a small-scale production company in 2002.
Our basis is custom-made products for lifescience research and industries in Denmarkand abroad. As a consequence our presentproduct line comprises product combina-tions originally introduced by Ampliqon aswell as products not offered anywhere else.
EXAMPLES ARE:
GLYCEROL-FREE TAQdeveloped for the North American market
AMPLIQ GENOMIC AMPLIFIERdeveloped for Statens Serum Institut (The Danish Serum Institute)
HOT START MASTER MIX BLUEdeveloped for the European market
Today, we are a well consolidated companywith a loyal and growing customer networkand a highly skilled laboratory staff.
We focus on two consistent ideals: flexibility and service.
We welcome individual customer requests,offer prompt product and technical supportand meet any enquiry with swift attention.
SMALL-SCALE AND SMALL BATCHWe base our procedures on small batchproduction. This way we stay in control ofthe enzyme purification process and ensurethe same quality of each product.
Ampliqon PCR enzymes are used in a multitude of settings to make DNA copiesthousands of times. Therefore, our respon-sibility is to offer PCR enzymes that youcan trust for reliable and successful DNAamplification.
THIS CATALOQUEThe purpose of this catalogue is to offeryou a handy overview of our PCR productline.
The catalogue is supplemented by a separate price list.
We hope that our catalogue conveys thatwe are proud to be your supplier of highquality PCR enzymes.
Kind regards
AMPLIQON A/S
A Danish manufacturer of bioreagents and PCR enzymes
DEAR CUSTOMER
Why buy Ampliqon PCR enzymes?
Ampliqon page 2
TEXT Birgitte Vedelstorp Andersen LAYOUT [ di’zain ] Anne Mette Jensen IMPRINT Ampliqon A/S, Skovlunde PRINTER’S FROM grafisk as, Kolding Denmark 20102nd impression
page 3 Ampliqon
WHOLE GENOME AMPLIFICATION
4 AmpliQ Genomic Amplifier
PCR AMPLIFICATION
HOT START
6 TEMPase Hot Start DNA Polymerase
8 TEMPase Hot Start Master Mix
9 TEMPase Hot Start Master Mix Blue
REAL TIME
10 RealQ PCR Master Mix
HIGH FIDELITY
11 AccuPOL DNA Polymerase
TAQ
12 Taq DNA Polymerase
15 Taq RedPOL DNA Polymerase
16 Taq DNA Polymerase Master Mix
18 Taq DNA Polymerase Master Mix Red
NUCLEOTIDES
19 dNTP Mix
19 dNTP Set
19 dATP, dCTP, dGTP and dTTP
ISOTHERMAL AMPLIFICATION
AMPLIFICATION AT ONE TEMPERATURE
21 OneTEMP custom-made
22 PRACTICAL INFORMATION
23 AMPLIQON BIOREAGENTS
Product catalogue
Ampliqon page 4
WHEN DO YOU USE AMPLIQ GENOMIC AMPLIFIER?If you have too little DNA to run a PCR. The AmpliQ Genomic Amplifier is a whole genomeamplification kit which generates an unlimited source of representative DNA from limitedsource material. All your DNA input is amplified. The AmpliQ Genomic Amplifier is suited fora variety of genetic studies such as genetic analysis, DNA storing, library construction andforensic work.
FEATURES
Ideal for single cell PCR
No additional primers needed
From pg template DNA to µg DNA in a 3-6 hour incubation at 30°C
DESCRIPTIONThe AmpliQ Genomic Amplifier exponential-ly amplifies single or double stranded linearDNA templates during an isothermal strand
displacement reaction, and the DNA gene-rated is of high molecular weight. A minorpart of the DNA will be single stranded – the major part double stranded.
Most DNA purification methods can beused to generate a starting template. Inmany cases unpurified cell lysates can bedirectly used as starting material.
The performance of AmpliQ Genomic Amplifier at isothermal temperature (30°C): illustrated by nine concentrations ofhuman genomic DNA ranging from 12.5 ng to 49 pg.
AMPLIQ GENOMIC AMPLIFIER
WHOLE GENOME AMPLIFICATION
AmpliQ Genomic Amplifier
AMPLIQ GENOMIC AMPLIFIER
PRODUCT NUMBER
280801 25 reactions
280803 100 reactions
280805 500 reactions
Ampliqon page 4
Fluorescence (dR)60000
50000
40000
30000
20000
10000
0 0 60 60 120 180180 240 300300 360 420 420 480 540540 600 660660 720 780780 840 900900 960 10201020 1080 1140 1140 1200 1261260 Minuts
NTC (no template control) 12.5 ng gDNA 6.25 ng gDNA
3.13 ng gDNA 1.56 ng gDNA 781 pg gDNA 391 pg gDNA
195 pg gDNA 98 pg gDNA 49 pg gDNA
page 5 Ampliqon
WHEN DO YOU USE TEMPASE HOT START DNA POLYMERASE?TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplificationand to diminish the formation of non-specific priming events during set-up. TEMPase HotStart DNA Polymerase allows for detection of low target copy numbers and for multiplexPCR in a single reaction tube.
FEATURES
Reaction set-up at room temperature
INCREASED
Sensitivity
Specificity
Product yield
DESCRIPTIONTEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNAPolymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which rendersthe enzyme inactive at room temperature.Thus, during set-up and the first ramp ofthermal cycling, the enzyme is not activeand misprimed primers are not extended.
Once the reaction reaches optimal activa-ting temperature, the chemical moiety iscleaved during a heat activation step, releasing the active TEMPase Hot StartDNA Polymerase into the reaction.
HOT START
TEMPase Hot Start DNA Polymerase
TEMPASE HOT START DNA POLYMERASE
PRODUCT NUMBER
Buffer I & II
220302 250 units
220303 500 units
220304 1000 units
220305 2000 units
220306 2500 units
220307 5000 units
220308 10000 units
220315 50000 units
Buffer II
232702 250 units
232703 500 units
232704 1000 units
232706 2500 units
Ampliqon page 6
page 7 Ampliqon
Ampliqon Competitor Q Competitor P Competitor I Competitor F Competitor FS
Chemical Antibody Antibody Chemical Genetic modified based hot modified hot modified hot modified hot
Taq start Taq start Taq start Taq start Taq
Comparison with and without hot start activation: Ampliqon TEMPase Hot Start DNA Polymerase and competitors.Amplification of a 210 bp fragment of the phenylalanine hydroxylase (PAH) gene conducted according to supplier’sinstructions.
Comparison with and without hot start activation: TEMPase Hot Start DNA Polymerase and competitor Q.Amplification has been conducted according to supplier’s instructions.
AMPLIFICATION WITH AND WITHOUT HOT START
Fluorescence (dRn)4.7
3.7
2.7
1.7
0.7
-0.31 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 Cycles
10 units TEMPase, 100 ng DNA, hot start 10 units TEMPase, 100 ng DNA, no hot start
5 units TEMPase, 100 ng DNA, hot start 5 units TEMPase, 100 ng DNA, no hot start
10 units competitor Q, 100 ng DNA, hot start 10 units competitor Q, 100 ng DNA, no hot start
5 units competitor Q, 100 ng DNA, hot start 5 units competitor Q, 100 ng DNA, no hot start
WHEN DO YOU USE TEMPASE HOT START MASTER MIX?TEMPase Hot Start 2 x Master Mix is a ready to use mix that offers easy reaction assemblyat room temperature prior to hot start PCR. Just add template and primers. TEMPase Hot Start 2 x Master Mix is an alternative to TEMPase Hot Start DNA Polymerase and isapplicable for primer extensions and other amplification purposes.
FEATURES
Minimal optimisation
Convenient reaction set-up
Fewer pipetting steps
Consistent test results
DESCRIPTIONAmpliqon TEMPase Hot Start DNAPolymerase, the NH4+ buffer system,dNTPs and magnesium chloride are present in TEMPase Hot Start 2 x MasterMix with TEMPase Buffer I or TEMPaseBuffer II.
The TEMPase buffer system covers all PCRapplications and allows your choice of buf-fer to be determined by your amplificationneeds.
HOT START
TEMPase Hot Start 2 x Master Mix
TEMPASE HOT START 2 X MASTER MIX
PRODUCT NUMBER
Buffer I
230301 100 reactions
230303 500 reactions
230304 1000 reactions
230306 2500 reactions
Buffer II
230701 100 reactions
230703 500 reactions
230704 1000 reactions
230706 2500 reactions
Ampliqon page 8
Ampliqon page 9
HOT START
TEMPase Hot Start 2 x Master Mix Blue
WHEN DO YOU USE TEMPASE HOT START MASTER MIX BLUE?The blue loading dye facilitates direct gel loading and eliminates the need for separate
loading dye for electrophoresis and visualisation after PCR. TEMPase Hot Start 2 x MasterMix Blue is an alternative to TEMPase Hot Start 2 x Master Mix with extra
time saving advantages.
FEATURES
Inactive at room temperature
Premixed 2 x solution
DESCRIPTIONAmpliqon TEMPase Hot Start DNAPolymerase, dNTPs, a blue loading dyeand magnesium chloride are present inTEMPase Hot Start 2 x Master Mix Blue with TEMPase Buffer I (balancedKCl/(NH4)2SO4 buffer system) or TEMPaseBuffer II ((NH4)2SO4 buffer system).
The two TEMPase buffers cover all PCRapplications and allow your choice of bufferto be determined by your amplificationneeds.
Comparison with and without 15-minute hot start activation. TEMPase Hot Start DNA Polymerase (lanes 1 and 2).TEMPase Hot Start 2 x Master Mix Blue (lanes 3 and 4). A DNA marker (lane 5).
TEMPASE COMPARISON
Lane 1 2 3 4 5
TEMPASE HOT START 2 X MASTER MIX BLUE
PRODUCT NUMBER
Buffer I
290401 100 reactions
290403 500 reactions
290404 1000 reactions
290406 2500 reactions
Buffer II
290801 100 reactions
290803 500 reactions
290804 1000 reactions
290806 2500 reactions
page 9 Ampliqon
Ampliqon page 10
WHEN DO YOU USE REALQ PCR MASTER MIX?Quantitative polymerase chain reaction (qPCR) is the preferred method for gene analysis. RealQ PCR 2 x Master Mix is well suited for gene expression, genotyping, SNP analysis,pathogen detection, drug target validation and for measuring RNA interference.
FEATURES
High sensitivity
High reproducibility
Wide dynamic range
Premixed 2 x solution
OPTIMUM PERFORMANCE ON STANDARD REAL TIME INSTRUMENTS:
ABI PRISM™
Roche LightCycler™
Mx3000™
Comparison between RealQ PCR 2 x Master Mix and competitor A. Amplification of a 130 bp GAPDH gene fragmentfrom 5 ng of human genomics DNA conducted according to supplier’s instructions.
A variety of additional real time kits are available.Please contact Ampliqon for more information.
DESCRIPTIONRealQ PCR 2 x Master Mix has undergoneextensive validation to ensure high perfor-mance.
Our RealQ kits contain TEMPase Hot StartDNA Polymerase, green dye and ROX refe-rence dye.
RealQ PCR Master Mix for probe is alsoavailable.
REAL TIME
RealQ PCR 2 x Master Mix
Fluorescence (dRn)11.4
10.4
9.4
8.4
7.4
6.4
5.4
4.4
3.4
2.4
1.4
0.4
-0.61 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Cycles
RealQ PCR Master Mix RealQ PCR Master Mix
Competitor A Competitor A
AMPLIFICATION PLOT
REALQ PCR 2 X MASTER MIX
PRODUCT NUMBER
For probe
250407 200 reactions
250427 2000 reactions
With green dye
250507 200 reactions
250527 2000 reactions
page 11 Ampliqon
HIGH FIDELITY
AccuPOL DNA Polymerase
WHEN DO YOU USE ACCUPOL DNA POLYMERASE?AccuPOL DNA Polymerase has proofreading abilities and is targeted at PCR applications,
which require high fidelity or blunt endings.
FEATURES
AccuPOL DNA Polymerase is a thermo-stable enzyme
Processes <3 kb
DESCRIPTIONAccuPOL DNA Polymerase exhibits both 5’ 3’ DNA polymerase activity and 3’ 5’proofreading exonuclease activity.AccuPOL kits include an extra 25 mMMgCl2 concentration if higher MgCl2 is required for specific reactions.
AccuPol DNA Polymerase comes in analternative Tween free version.
* The error rate equals mutation frequency per base per duplication.
FIDELITY COMPARISONS OF THERMOSTABLE DNA POLYMERASES USING THE LACIOZ ASSAY
ENZYME ACCUPOL PFU TAQ
Error rate* (fidelity) 1.1 x 10-6 1.3 x 10-6 18.0 x 10-6
Elongation rate (bases/sec) 30 25 61
ACCUPOL DNA POLYMERASE
PRODUCT NUMBER
Standard high fidelity
210302 250 units
210303 500 units
210304 1000 units
210306 2500 units
Tween free
210402 250 units
210403 500 units
210404 1000 units
210406 2500 units
WHEN DO YOU USE TAQ DNA POLYMERASE?For routine PCR applications, which require high yield and reliable DNA amplification.
FEATURES
A thermostable recombinant DNApolymerase
Enzyme isolated from Thermus Aquaticus
Molecular weight of approximately 95 kDa
Leaves an A’ overhang
DESCRIPTIONAmpliqon Taq DNA Polymerase exhibitsboth a 5’ 3’ DNA polymerase and a 5’ 3’ exonuclease activity. Taq DNAPolymerase has no proofreading ability.
TAQ
Taq DNA Polymerase
AMPLIQON TAQ DNA POLYMERASE
PRODUCT NUMBER
Glycerol free
100103 500 units
100104 1000 units
Standard Taq
110102 250 units
110103 500 units
110104 1000 units
110106 2500 units
110107 5000 units
110108 10000 units
PRODUCT NUMBER
Mg free and Standard Buffer
110203 500 units
110204 1000 units
110208 10000 units
Tween and Triton free
110303 500 units
110304 1000 units
110308 10000 units
Combination Buffer
110403 500 units
110404 1000 units
110406 2500 units
110408 10000 units
PRODUCT NUMBER
Mg free and Ammonium Buffer
110503 500 units
110504 1000 units
110506 2500 units
110508 10000 units
20 mM MgCl2 and Ammonium Buffer
111304 1000 units
+ dNTP
115102 250 units
115103 500 units
115104 1000 units
115108 10000 units
Ampliqon page 12
page 13 Ampliqon
ENZYME DILUTION Comparison between Ampliqon Taq DNA Polymerase and four competitors.Amplification of a 160 bp fragment of the human phenylalanine hydroxylase (PAH) gene. Each amplification has been conducted according to supplier’s instructions.
Enzyme dilution
COMPARISON BETWEEN AMPLIQON TAQ DNA POLYMERASE AND COMPETITORS
Enzyme dilution
Ampliqon
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
Competitor I
Ampliqon Competitor F
Ampliqon Competitor B
Ampliqon Competitor A
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
Ampliqon page 14Ampliqon page 14
page 15 Ampliqon
WHEN DO YOU USE TAQ REDPOL DNA POLYMERASE?Taq RedPOL DNA Polymerase is an alternative to standard Taq DNA Polymerase.
The red dye provides convenient identification of enzyme tube and confirms complete mixing.
FEATURES
With Ammonium Buffer
DESCRIPTIONIn Ampliqon Taq RedPOL DNA Polymerasethe inert dye has no effect on downstreamprocesses. Taq RedPOL DNA Polymeraseis added directly to the reaction mix.
TAQ
Taq RedPOL DNA Polymerase
TAQ REDPOL DNA POLYMERASE
PRODUCT NUMBER
200303 500 units
200304 1000 units
200306 2500 units
200307 5000 units
200308 10000 units
200309 20000 units
Ampliqon page 16Ampliqon page 16Ampliqon page 16
WHEN DO YOU USE TAQ DNA POLYMERASE MASTER MIX?Taq DNA Polymerase Master Mix is an alternative to Taq DNA Polymerase. Taq DNAPolymerase Master Mix offers reduced set-up time and eliminates the risk of contaminationof reaction components. Fewer handling steps lead to better reproducibility, which makes Taq DNA Polymerase Master Mix suitable for standard tests.
FEATURES
Premixed solution
Minimal optimisation
Fewer pipetting steps
DESCRIPTIONTaq DNA Polymerase Master Mix is a readyto use 1.1 x or 2.0 x reaction mix. TaqDNA Polymerase Master Mix is composedof Ampliqon Taq DNA Polymerase, theNH4+ buffer system, dNTPs and magne-sium chloride.
TAQ
Taq DNA Polymerase Master Mix
TAQ DNA POLYMERASE MASTER MIX
PRODUCT NUMBER
1.1 x and 1.5 MgCl2
120301 100 reactions
120303 500 reactions
120306 2500 reactions
1.1 x and 2.0 mM MgCl2
130301 100 reactions
130303 500 reactions
130306 2500 reactions
2.0 x and 1.5 mM MgCl2
140301 100 reactions
140303 500 reactions
140306 2500 reactions
2.0 x and 2.0 mM MgCl2
150301 100 reactions
150303 500 reactions
150306 2500 reactions
page 17 Ampliqonpage 17 Ampliqon
Ampliqon page 18
WHEN DO YOU USE TAQ DNA POLYMERASE MASTER MIX RED?Taq DNA Polymerase Master Mix Red is an alternative to Taq DNA Polymerase Master Mixwith the same advantages. The inert red dye and stabiliser are extra features, which allowfor direct loading onto your agarose gel for analysis.
FEATURES
No need for separate loading dye
Taq DNA Polymerase Master Mix Red is ready-made for electrophoresis and visualisation
Fewer handling steps
DESCRIPTIONTaq DNA Polymerase Master Mix Red is aready to use 1.1x or 2.0x reaction mix. Taq DNA Polymerase Master Mix Red isavailable in two final MgCl2 concentrations:1.5 mM and 2.0 mM.
Taq Polymerase Master Mix Red is com-posed of Ampliqon Taq DNA Polymerase,the NH4+ buffer system, dNTPs and magnesium chloride.
TAQ
Taq DNA Polymerase Master Mix Red
TAQ DNA POLYMERASE MASTER MIX RED
PRODUCT NUMBER
1.1 x and 1.5 mM MgCl2
160301 100 reactions
160303 500 reactions
160306 2500 reactions
1.1 x and 2.0 mM MgCl2
170301 100 reactions
170303 500 reactions
170306 2500 reactions
2.0 x and 1.5 mM MgCl2
180301 100 reactions
180303 500 reactions
180306 2500 reactions
2.0 x and 2.0 mM MgCl2
190301 100 reactions
190303 500 reactions
190306 2500 reactions
Ampliqon page 18Ampliqon page 18
Taq DNA Polymerase Master Mix Red is an Ampliqonbest seller.
page 19 Ampliqon
NUCLEOTIDES
DNTP MIX
PRODUCT NUMBER
500005 25 mM / 2 x 1 ml
501004 12.5 mM / 2 x 500 µl
501007 12.5 mM / 8 x 500 µl
502003 10 mM / 1 x 500 µl
502004 10 mM / 2 x 500 µl
502005 10 mM / 5 x 500 µl
502007 10 mM / 8 x 500 µl
503009 2.5 mM / 10 x 1 ml
DNTP SET
PRODUCT NUMBER
511104 100 mM / 4 x 250 µl
511107 100 mM / 16 x 250 µl
511109 100 mM / 80 x 250 µl
511120 100 mM / 4 x 2 ml
DATP, DCTP, DGTP AND DTTP
PRODUCT NUMBER
521102 25 µmol / 1 x 250 µl
521202 25 µmol / 1 x 250 µl
521302 25 µmol / 1 x 250 µl
521402 25 µmol / 1 x 250 µl
DNTP
FEATURES
PCR amplification components
For DNA polymerisation, DNA labelling and sequencing processes
High purity: >98% by HPLC
pH 7.5
dNTP Mix
Ready to use solution
Mix of dATP, dCTP, dGTP and dTTP
dNTP Set
Set of dATP, dCTP, dGTP and dTTP
dATP, dCTP, dGTP and dTTP
Either dATP, dCTP, dGTP or dTTP
Ampliqon page 20Ampliqon page 20Ampliqon page 20
WHAT IS ONETEMP?OneTEMP is a generic term for a custom-made series of DNA/RNA amplification kits fordetection of bacteria, parasites and viruses. A common feature of the OneTEMP series
is that thermocycling is made superfluous. OneTEMP covers ten different isothermal technologies, which are as sensitive and specific as standard PCR reactions and in addition often more tolerant to biological components. Isothermal amplification
is a time and cost saving alternative to most PCR set-ups.
WHY AMPLIFICATION AT ONE TEMPERATURE?The DNA amplification kinetics in isothermalOneTEMP is a function of the reagent com-ponents themselves. This feature makesyour laboratory work independent of equip-ment limitations.
REAL TIME DETECTIONThe OneTEMP technologies can be transformed into traditional real time kits applicable to standard laboratory fluorescence detection and point of caredevices.
ABOUT ONETEMPYou have a PCR reaction that you want to transform into an isothermal version.Provide us with primers and template andwe will find the right isothermal amplifica-tion technology for your need.
ONETEMP IS SUITABLE FOR
Genomics amplification
cDNA/RNA amplification
REQUIRES
From one to four primers
Between 37˚C - 65˚C
From one to several enzymes (enzyme mixes)
Some kits require denaturation steps
HOW?The Ampliqon science team offers expertknowledge in transforming your PCR reaction into an isothermal version suitablefor your specific need and set-up.
FOR MORE INFORMATION: OneTEMP@ampliqon.com
NOTE: your present PCR reaction analoguemust already be working in your laboratory.
ISOTHERMAL AMPLIFICATION
OneTEMP custom-made
Pro
duct
exa
mpl
e
page 21 Ampliqon
Ampliqon page 22
PRICESPlease see our separate price list issuedwith this catalogue.
SAMPLES
In connection with orders
Limited quantity
No dNTP samples
No Genomic Amplifier samples
SAMPLE SIZE
20 reactions
25 units
50 units
HOW TO ORDEREmail: enzyme@ampliqon.comFax: +45 70201179
Order confirmation and shipping notification within 24 hours.
SHIPPING ON DRY ICE
International: air freight
Denmark: road or rail freight
Shipping fee depends on shipping agent, destination and weight
PACKAGING
Minor fee for packaging and dry ice depending on quantity and box size (small, medium and large box sizes)
CONTACTAmpliqon A/SLiterbuen 11DK-2740 SkovlundeDenmarkwww.ampliqon.cominfo@ampliqon.com
Phone: +45 70201169Fax: +45 70201179
VAT No.: 26388899
AMPLIQON BANK INFORMATIONNykredit Bank A/SErhvervs- og investeringscenter Glostrup
Hovedvejen 108DK-2600 GlostrupDenmark
Account: 5470-7025886Swift: NYKBDKKKIban: DK8854700007025886
Practical information
page 23 Ampliqon
In addition to our PCR enzyme production,Ampliqon is the supplier of a comprehen-sive bioreagent product line.
We manufacture about 10000 different bioreagents for Danish hospitals, life science institutions and industries. Allcustom-made and on demand.
Ampliqon bioreagents
SOME EXAMPLES:Aga ELISA wash buffer, ammonium acetate in MilliQ water, barbiturate buffer, blood beads, bromine water,DEPC water, buffered formalin, glucose,chloramphenicol, potassium chloride,artificial sweat, artificial urine, sodiumacetate, paracetamol, redistilled water,salicylic acid, sterile buffer glass, swabfluid, sulfuric acid, silver nitrate, urologi-cal fixative, vaseline, cotton swab, yeast extract agar …
AMPLIQON A/S
Literbuen 11
DK-2740 Skovlunde
Denmark
www.ampliqon.com
info@ampliqon.com
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