Slide 1Rob Brown Slide 2 To think about how we can save money in the lab. To discuss how Haemochromatosis testing has changed and developed over time in Newcastle and how…

Slide 1Oligo 7 Primer Analysis Software Rules for PCR Primer Selection and Presentation of the Software 2010 Molecular Biology Insights, Inc. Slide 2 Contents 1. PCR Primer…

Slide 1PCR for Aquatic Animal Health Web Training Module V1; August 2011 Created by Maureen Purcell, Ph.D. Slide 2 Goal To provide an overview of PCR-based diagnostic assays…

Slide 1Real-Time PCR combines DNA amplification with real time amplified product detection in a single tube. More specific then gel or hybridization assays. Less time consuming…

Slide 1PCR Techniques Slide 2 Basics of PCR Primers –15-60bp (60bp is limit synthesized by IDT) –Annealing temp ideally >55C (portion that anneals to your template)…

Slide 1Recombinant DNA Technology Slide 2 Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.…

Slide 1DNA Manipulation in Synthetic Biology Slide 2 DNA structure Covalent bondsHydrogen bonds Slide 3 DNA  RNA  protein Slide 4 Levels of Abstraction Slide 5 Manipulation…

1.Patrick MerelBiomedical Innovation Platform (PTIB), Pessac, France [email protected] Procedures Automation in Molecular Diagnostic part 312. 2 Options for Post-PCR…

1.LECTURE 5 Polymerase Chain Reaction2. Amplifying DNA in Vitro: The Polymerase Chain Reaction (PCR)  The polymerase chain reaction, PCR, canproduce many copies of a specific…

1.Recombinant DNA Technology2. Needles in Haystacks How to find one gene in large genome?A gene might be 1/1,000,000 of the genome.Three basic approaches: 1.Polymerase chain…