ZytoDot Probes for Chromosome Enumeration · chromosomes 13 and 21 share the same repetitive sequences, they cannot be differ-entiated by probes detecting centromere specific repeats.

ZytoVision GmbH · Fischkai 1 · 27572 Bremerhaven · Germany · www.zytovision.com242 242DE012-1-20

BackgroundThe ZytoDot ® Chromosome Enumeration Probes are designed for identification and enumeration of human chromosomes in in-terphase cells and as an adjunct to stand-ard karyotyping in metaphases. These probes will produce sharp, bright signals specific for each individual chromosome.

CEN Probe DescriptionFor most chromosomes, direct labeled ZytoDot ® CEN™ Probes hybridizing to highly repetitive human satellite DNA se-quences mainly located at the centromeric regions of chromosomes are applicable.

SPEC Probe DescriptionAs several chromosomes share the same repetitive sequences resulting in cross-hy-bridization signals, they cannot be differ-entiated by centromere specific probes. Instead these chromosomes can be iden-tified by direct labeled ZytoDot ® SPEC™ Probes hybridizing in close proximity to the respective satellite DNA sequences or to other chromosome specific loci.

ResultsIn a normal interphase nucleus, two sig-nals are expected using Chromosome Enu-meration Probes specific for autosomes. Using chromosome Y specific probes will result in normal male cells in one signal and in normal female cells in no signal. Using chromosome X specific probes will result in normal male cells in one signal and in normal female cells in two signals per nucleus. Other signal patterns indicate numerical aberrations of the respective chromosome.

ZytoDot ® SPEC Probe Maps

The ZytoDot ® SPEC 1p12 Probe is designed to hybridize in close proxim-ity of centromere 1 at 1p12 harboring the WARS2 gene. Since chromosomes 1, 5, and 19 share the same repetitive sequences, they cannot be differentiated by probes detecting centromere specific repeats.

The ZytoDot ® SPEC 2q11 Probe is specific for the AFF3 (AF4/FMR2 family, member 3) gene region in 2q11.2. Due to cross-hybridizations of chromosome 2 al-pha satellites to other centromeric regions, probes specific for 2q11 are frequently used for chromosome 2 copy number detection.

The ZytoDot ® SPEC 13q12 Probe is designed to hybridize in close proximity of centromere 13 at 13q12.11. Since chromosomes 13 and 21 share the same repetitive sequences, they cannot be differ-entiated by probes detecting centromere specific repeats.

The ZytoDot ® SPEC 21q22 Probe hybrid-izes to the so-called Down Syndrome Criti-cal Region on 21q22.13-q22.2 commonly duplicated in cases with partial trisomy 21. Since chromosomes 13 and 21 share the same repetitive sequences, they cannot be differentiated by probes detecting centromere specific repeats.

ZytoDot ® Probes for Chromosome Enumeration

SPEC 13q12 Probe map (not to scale).

Cen Tel13q12.11

RH98442 D13S1546

~165 kb

SPEC 1p12 Probe map (not to scale).

Tel Cen1p12

SHGC-141884 RH105871

~285 kb

WARS2

5’3’

HAO2

3’5’

SPEC 2q11 Probe map (not to scale).

Cen Tel2q11.2

RH69575 RH121214

~275 kb

AFF3

5’3’

SPEC 21q22 Probe map (not to scale).

Cen Tel21q22.13-q22.2

SHGC-170072 D21S338

~200 kb

ZytoVision GmbH · Fischkai 1 · 27572 Bremerhaven · Germany · www.zytovision.com

* Using 10 µl probe solution per test. only available in certain countries. All other countries research use only! Please contact your local dealer for more information.

243 243DE012-1-20

Prod. No. Product Alpha/Class. Sat. Chr. Band Label Tests* (Volume)C-3035-400 ZytoDot SPEC 1p12 Probe - 1p12 DIG 40 (400 μl)

C-3051-400 ZytoDot SPEC 2q11 Probe - 2q11.2 DIG 40 (400 μl)

C-3045-400 ZytoDot CEN 3 Probe D3Z1 3p11.1-q11.1 DIG 40 (400 μl)

C-3002-400 ZytoDot CEN 6 Probe D6Z1 6p11.1-q11 DIG 40 (400 μl)

C-3008-400 ZytoDot CEN 7 Probe D7Z1 7p11.1-q11.1 DIG 40 (400 μl)

C-3016-400 ZytoDot CEN 8 Probe D8Z2 8p11.1-q11.1 DIG 40 (400 μl)

C-3014-400 ZytoDot CEN 12 Probe D12Z3 12p11.1-q11 DIG 40 (400 μl)

C-3052-400 ZytoDot SPEC 13q12 Probe - 13q12.11 DIG 40 (400 μl)

C-3006-400 ZytoDot CEN 17 Probe D17Z1 17p11.1-q11.1 DIG 40 (400 μl)

C-3026-400 ZytoDot SPEC 21q22 Probe - 21q22.13-q22.2 DIG 40 (400 μl)

C-3025-400 ZytoDot CEN X Probe DXZ1 Xp11.1-q11.1 DIG 40 (400 μl)

C-3020-400 ZytoDot CEN Yq12 Probe III DYZ1 Yq12 DIG 40 (400 μl)

C-3048-400 ZytoDot 2C CEN X/Y Probe DXZ1/DYZ3 Xp11.1-q11.1/Yp11.1-q11.1 DNP/DIG 40 (400 μl)

Related ProductsC-3018-40 ZytoDot CISH Implementation Kit 40 Incl. Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml; Wash Buffer SSC, 560 ml; PBS/Tween, good for 2000 ml; Blocking Solution, 4 ml; Mouse-anti-DIG, 4 ml;

Anti-Mouse-HRP-Polymer, 4 ml; DAB Solution A, 0.3 ml; DAB Solution B, 10 ml; Mayer’s Hematoxylin Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

C-3044-40 ZytoDot 2C CISH Implementation Kit 40 Incl. Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml; Wash Buffer SSC, 560 ml; 20x Wash Buffer TBS, 2x 50 ml; Anti-DIG/DNP-Mix, 4 ml; HRP/AP-Polymer-Mix, 4 ml;

AP-Red Solution A, 0.4 ml; AP-Red Solution B, 15 ml; HRP-Green Solution A, 0.8 ml; HRP-Green Solution B, 15 ml; Nuclear Blue Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

Normal nuclei each with two CEN 12 signals. CEN X/Y Probe hybridized on normal female interphase cells as indicated by two red

(chromosome X) signals per nucleus.

CEN X/Y Probe hybridized on normal male interphase cells as indicated by one red (chromosome X) and one green (chromosome Y) signal per nucleus.