Application Note ZEISS LSM 800 and LSM 880 with Airyscan Imaging Biological Samples – a Reference List
Application Note
ZEISS LSM 800 and LSM 880 with AiryscanImaging Biological Samples – a Reference List
Application Note
2
ZEISS LSM 800 and LSM 880 with AiryscanImaging Biological Samples – a Reference List
Author: Dr. Annette Bergter Carl Zeiss Microscopy GmbH, Germany
Date: December 2016
Introduction
The Confocal Laser Scanning Microscope (LSM) has become
one of the most popular instruments for fluorescence imaging
in biomedical research, because it affords researchers images
with high contrast and a versatile optical sectioning capability
to investigate three dimensional biological structures [1].
The optical sectioning ability of an LSM is a product of
scanning a focused laser spot, across a sample to create an
image one point at a time. The generated fluorescence from
each point is collected by the imaging objective and results
from fluorophores in the sample that reside both in the
desired plane of focus and in out of focus planes. In order
to segregate the fluorescence emitted from the desired focal
plane, an aperture (pinhole) is positioned in the light path
to block all out of focus light from reaching the detector
(traditionally a PMT) [2].
The traditional principle of the LSM beampath forces the
user to compromise either on resolution or sensitivity.
Resolution is increased by closing the pinhole, at the same
time limiting the amount of light that is allowed to pass
through to the detector. The Airyscan detector of LSM 880
and LSM 800 overcomes this challenge. The detector
consists of 32 GaAsP PMT detector elements, which are
arranged in a hexagonal array (Figure 1), positioned at a
conjugated focal plane in the beam path the detector is
functioning as the traditional LSM pinhole. This design
makes it possible to collect more light (equivalent to a
pinhole opened to 1.25 AU), whilst at the same time
dramatically enhancing the resolution, with every detector
element acting as an efficient pinhole with a dia meter of
only 0.2 Airy Unit (AU). Instead of facing an either / or
decision, a simultaneous enhancement of resolution by the
factor of 1.7× and signal-to-noise by 4 – 8× was introduced
to LSM imaging. Detailed descriptions of the theory and
techno logy of Airyscanning can be found in separate tech-
nology notes [3, 4, 5].
As an area detector, Airyscan can capture spatial information
that is utilized to parallelize the scanning process, collecting
4 image lines simultaneously in the Fast mode (Figure 2).
This means enhancing acquisition speed by a factor of 4
while keeping high pixel dwell times to efficiently collect
emitted photons. In standard mode, the focused laser beam
is moved along the x-axis to acquire one image line, before
it is moved in the y-axis to acquire the consecutive image
line. In Fast mode imaging, four image lines are acquired at
the same time when moving the laser in the x-direction.
This publication list 1 assembles some of the scientific work
that has been done with LSM 880 and 800 systems. The great
variety of applications collectively profits from the light
efficient beam path of the LSM systems, and the unique
combination of superresolution, high sensitivity and high
speed imaging provided by Airyscan.
Learn more about LSM 800, click here
Learn more about LSM 880, click here
Further Reading:
[1] Conchello, J.-A. and Lichtman, J.W., Optical sectioning microscopy. Nature methods, 2005. 2(12): p. 920-931.
[2] Minsky, M., Memoir on inventing the confocal scanning microscope. Scanning, 1988. 10(4): p. 128-138.
[3] Huff, J., The Airyscan detector from ZEISS: confocal imaging with improved signal-to-noise ratio and super-resolution. Nature methods, 2015. 12.
[4] Weisshart, K., The basic principle of Airyscanning. 2014. ZEISS Technology Note
[5] Huff, J.; Bathe, W.; Netz, R.; Anhut, T.; Weisshart, K., The Airyscan detector from ZEISS. Confocal imaging with improved signal-to-noise
ratio and superresolution. 2015. ZEISS Technology Note
Application Note
3
4. Airy disk 5. Airyscan detector
1. Mirror2. Emission filters3. Zoom optics
1
2
3
4 5
4
4. Airy disk 5. Airyscan detector
1. Mirror2. Emission filters3. Zoom optics
1
2
3
4 5
4
Figure 1 Beampath Airyscan Figure 2 Airyscan Fast mode
Cell culture / In vitro / Fixed cells
Publication Journal Sample Application System
2016. C.A. Casey et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. doi:10.1111/acer.13247
AlcoholismClinical and Experimental Research
HepG2 cells transfected with mouse ADH1 (VA-13 cells)Rat hepatocytesRat liver tissue sections
Fluorescent Immuno-histochemical staining
LSM 800Airyscan
2016. S.G.B. Furness et al. A novel ligand of calcitonin receptor reveals a potential new sensor that modulates programmed cell death. doi:10.1038/cddiscovery.2016.62
Nature - Cell Death Discovery
MG 63 (Human osteosarcoma cell line)A172 (Human glioblastoma cell line) GBM-L2 ( high-grade glioma cell line)
Fluorescent Immuno-histochemical staining
LSM 800Airyscan
2016. P. Robinson et al. Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes. doi:10.1126/science.aaf0659
Science Cardiomyocyte cells Live cell imaging LSM 880Airyscan
2016. A. M. Ward et al. The Golgi associated ERI3 is a Flavivirus host factor. doi:10.1038/srep34379
NatureScientific Reports
HuH-7 cells Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. M.J. Patton et al. Chlamydial Protease-Like Activity Factor and Type III Secreted Effectors Cooperate in Inhibition of p65 Nuclear Translocation. doi:10.1128/mBio.01427-16
mBio HeLa cells Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. L. Ma et al. Exploring functional roles of TRPV1 intracellular domains with unstructured peptide-insertion screening. doi:10.1038/srep33827
NatureScientific Reports
Mouse TRPV1 cell line Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. E. Sosa et al. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. doi:10.1016/j.scr.2016.09.015
Stem Cell Research riPSC89 cells Fluorescent Immuno-histochemical staining
LSM 880
2016. O. Pylypenko et al. Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. doi:10.7554/eLife.17523
eLIFE HEK293 cellsWild-type C57BL/6
FLIM LSM 880PicoQuant
2016. K. Rao et al. Spastin, atlastin and ER relocalization are involved in axon, but not dendrite, regeneration. doi:10.1091/mbc.E16-05-0287
MBoC Molecular biology of the Cell
Drosophila LSM 880Airyscan
Application Note
4
Publication Journal Sample Application System
2016. F. Li et al. Reciprocal regulation of actin cytoskele-ton remodeling and cell migration by calcium and zinc: role of TRPM2 channels. doi:10.1242/jcs.179796
Journal of Cell Science HeLa cells PC-3 cells
Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. A. Petrosyan et al. The role of Rab6a and phosphorylation of non-muscle myosin IIA tailpiece in alcohol-induced Golgi disorganization. doi:10.1038/srep31962
NatureScientific Reports
HepG2 cells mouse ADH1 (VA-13 cells)
Fluorescent Immuno-histochemical staining
LMS 800 Airyscan
2016. P. Sheehan et al. Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway. doi:10.1523/JNEUROSCI.0725-16.2016
The Journal of Neuroscience
LSM 800Airyscan
2016. R. L. Weaver et al. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. doi:10.7554/eLife.16620
eLIFE Mouse embryonic fibroblasts (MEF)
Fluorescent Immuno-histochemical staining
LSM 880
2016. B. Yao et al. Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells. doi:10.1016/j.cellsig.2016.08.003
Cellular Signalling Liver cancer cells LSM 800
2016. M. Zhou et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. doi:10.1083/jcb.201604061
JBCThe Journal of Cell Biology
U2OS cells Live cell imaging LSM 800Airyscan
2016. E. Adu-Gyamfi et al. Flexibility of the head-stalk linker domain of paramyxovirus HN glycoprotein is essen-tial for triggering virus fusion. doi:10.1128/JVI.01187-16
Journal of Virology Paramyxoviridae LMS 800Airyscan
2016. L.Li et al. Real-time imaging of Huntingtin aggre-gates diverting target search and gene transcription.doi:10.7554/eLife.17056
eLIFE Mouse D3 (ATCC, Manassas, VA) ES cells
Fluorescent proteinsAiryscanSIMSingle molecule localizationFRAP
LSM 880Airyscan
2016. J. Bruno et al. SEC16A is a RAB10 effector required for insulin-stimulated GLUT4 trafficking in adipocytes. doi:10.1083/jcb.201509052
JBCThe Journal of Cell Biology
Adipocytes Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. X. Xie et al. Rational Design of an α-Ketoamide-Based Near-Infrared Fluorescent Probe Specific for Hydrogen Peroxide in Living Systems. doi:10.1021/acs.analchem.6b01256
Analytical Chemistry Cell culture LSM 880
2016. D.C. Gershlick et al. TSSC1 is Novel Component of the Endosomal Retrieval Machinery. doi:10.1091/mbc.E16-04-0209
MBoCMolecular Biology of the Cell
Mouse tissue extractsWT, TSSC1-KO and VPS54-KO HAP1 cell lines
Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016, T. Yang et al. A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echino-coccus multilocularis. doi:10.3347/kjp.2016.54.2.225
The Korean Journal of Parasitology
Echinococcus multilocularis Live cell imaging LSM 800
2016, J. J. Kelly et al. Specific functional pathologies of Cx43 mutations associated with oculodentodigital dysplasia. doi:10.1091/mbc.E16-01-0062
MBoCMolecular Biology of the Cell
Primary human fibroblasts culturesHuman ODDD patient skin biopsies
Fluorescent Immuno-histochemical staining
LSM 800 Airyscan
2016, B. M. Laitman et al. The transcriptional activator Krüppel-like factor -6 is required for CNS myelination. doi:10.1371/journal.pbio.1002567
PLOS, Biology MouseOligodendrocyte progenitors cell culture
Fluorescent Immuno-histochemical staining
LSM 880
2016, F. M. Kuhlmann et al. Blood Group O–Dependent Cellular Responses to Cholera Toxin: Parallel Clinical and Epidemiological Links to Severe Cholera. doi:10.4269/ajtmh.16-0161
ASTHM, The American Journal of Tropical Medicine and Hygiene
Human enteroid cultureCholera toxin
Live cell imagingCRISPR
LSM 880
2016, M. Kargar et al. Colloidal Crystals Delay Formation of Early Stage Bacterial Biofilms. doi:10.1021/acsbiomaterials.6b00163
ACSBiomaterialsScience and Engineering
Pseudomonas aeruginosaBiofilm
LSM 880
Cell culture / In vitro / Fixed cells
Application Note
5
Publication Journal Sample Application System
2016, W. Quing Liu et al. A potential peptide vector that allows targeted deliveryof a desired fusion protein into the human breast cancer cell line MDA-MB-231. doi:10.3892/ol.2016.4538
Oncology LettersSpandidos Publications
MDA-MB-231 cells Live cell imagingTime series
LSM 800
2016, Y. Xing-Mei et al. Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6. doi:10.3892/mmr.2016.5167
Molecular Medicine ReportsSpandidos Publications
Cultured podocytes Ca-Imaging (Fluo-3) Live cell imagingTime series
LSM 880
2016, D. Kalafatovic et al. MMP-9 triggered self-assem-bly of doxorubicin nanofiber depots halts tumor growth. doi:10.1016/j.biomaterials.2016.04.039
Biomaterials MDA-MB-231-luc-D3H2LN cell culture
CRISPRFluorescent Immuno-histochemical staining
LSM 880 Airyscan
2016, H. Zuh et al. Parkinson’s disease-like forelimb akinesia induced by BmK I, a sodium channel modulator. doi:10.1016/j.bbr.2016.04.036
Behavioural Brain Research
Human neuroblastoma SHSY5Y Fluorescent Immuno-histochemical staining
LSM 880
2016, M. O’Brien et al. CD4 Receptor is a key determinant of divergent HIV-1 sensing by plasmacytoid dendritic cells. doi:10.1371/journal.ppat.1005553
PLOS, Pathogenes HEK cellsPurified human pDC Incubated with GFP PR8 influenza 10 HAU, GFP Ha-HIV or GFP JRFL
Fluorescent Immuno-histochemical staining
LSM 880
2016, Y. Qiao et al. Melanoma cell adhesion molecule stimulates yes-associated protein transcription by enhancing CREB activity via c-Jun/c-Fos in hepatocellular carcinoma cells. doi:10.3892/ol.2016.4442
Oncology LettersSpandidos Publications
Human HCC Bel-7402, SMMC-7721 and Huh7 cells lines
Fluorescent Immuno-histochemical staining
LSM 800
2016, M. A. Rider et al. ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles. doi:10.1038/srep23978
NatureScientifc Reports
HEK293 cells Fluorescent Immuno-histochemical staining
LSM 880
2016, K. Jin et al. Multiphoton luminescent graphene quantum dots for in vivo tracking of human adipose-derived stem cells. doi:10.1039/C6NR02143C
Nanoscale MouseHuman stem cells
Fluorescent Immuno-histochemical staining
LSM 880
2016, L. Xiaojin et al. Long-term performance and microbial community characterization of an osmotic anammox system for removing reverse-fluxed ammoni-um. doi:10.1016/j.biortech.2016.03.137
Bioresource Technology Biofilm FISH LSM 880
2016, J. HoJun et al. Nanostructured surface of electro-spun PCL/dECM fibres treated with oxygen plasma for tissue engineering. doi:10.1039/C6RA03840A
RSC Advances Cell culture in PCL/d-ECM scaffold
Fluorescent Immuno-histochemical stainingTissue formation and regeneration
LSM 800
2016, L. Hyeongjin. A scaffold with a bio-mimetically de-signed micro/nano-fibrous structure using decellularized extracellular matrix. doi:10.1039/C5RA27845G
RSC Advances Cell culture in PCL/d-ECM scaffold
Fluorescent Immuno-histochemical stainingTissue formation and regeneration
LSM 800
2016, R. Guo et al. Porcine reproductive and respiratory syndrome virus utilizes nanotubes for intercellular spread. doi:10.1128/JVI.00036-16
Journal of Virology porcine reproductive and respi-ratory syndrome virus (PRRSV) in HEK-293T cells
Live cell imaging LSM 880
2016, T. Bacon et al. Histone deacetylase 3 indirectly modulates tubulin acetylation. doi:10.1042/BJ20150660
Biochemical Journal Immortalized human prostate cancer cells (PC3)Human HDAC3 clones
Fluorescent Immuno-histochemical staining
LSM 880 Airyscan
2016, S. Skariah et al. The FIKK kinase of Toxoplasma gondii is not essential for the parasite’s lytic cycle. doi:10.1016/j.ijpara.2016.01.001
International Journal for Parasitology
Toxoplasma gondii in HFF cells LSM 880 or 800Airyscan
2016, X. Li et al. Nitrogen removal by granular nitritation-anammox in an upflow membrane-aerated biofilm reactor. doi:10.1016/j.watres.2016.02.031
Water Research microorganisms (e.g. AOB, NOB and anammox bacteria) in the granule and biofilm
Microbial community analysis
LSM 880
2016, G.W. Dougherty et al. DNAH11 Localization in the Proximal Region of Respiratory Cilia Defines Distinct Outer Dynein Arm Complexes. doi:10.1165/rcmb.2015-0353OC
American Journal of Respiratory Cell and Molecular Biology
Trachearespiratory epithelium
LSM 880Apotome
Cell culture / In vitro / Fixed cells
Application Note
6
Publication Journal Sample Application System
2015, Kitamura et al. Interaction of RNA with a C-terminal fragment of the amyotrophic lateral sclerosis-associated TDP43 reduces cytotoxicity. doi:10.1038/srep19230
NatureScientific Report
Mouse neuroblastoma Neuro2A cells transiently expressing GFP, TDP43-GFP, GFP-TDP35, GFP-TDP25, or GFP-NLS-TDP25
Fluorescent Immuno-histochemical staining
LSM 800Airyscan
2016, A. Jassim et al. Engineered Cx40 variants increased docking and function of heterotypic Cx40/Cx43 gap junction channels.doi:10.1016/j.yjmcc.2015.11.026
Journal of Molecular and Cellular Cardiology
Mouse neuroblastoma (N2A)human cervical carcinoma (HeLa) cells
Localizations of Cx40-YFP, mutant-YFP, Cx40-RFP and Cx43-RFP were studied by expressing these con-structs individually in gap junction deficient HeLa and N2A cells.
LSM 800Airyscan
2015, Y. Suzuki-Karasaki et al. Distinct effects of TRAIL on the mitochondrial network in human cancer cells and normal cells: role of plasma membrane depolarization. doi:10.18632/oncotarget.4268
Oncotarget A549 cells (human lung ade-nocarcinoma epithelial cell line)Osteosarcoma cell linesHuman dermal fibroblasts from facial dermis
Mitochondrial network imaging and length mea-surements
LSM 880
2015, W. Ji et al. Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites. doi:10.7554/eLife.11553
eLIFE Human osteosarcoma U2OS Live cell imagingDrp 1 maturation
LSM 880Airyscan
2015, K. A. Makowska et al. Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells. doi:10.1016/j.celrep.2015.11.012
Cell Reports LNCaP, DU145, and PC-3 cells1535NP and CT cells
Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016, S. Kang et al. Development of anti-biofouling in-terface on hydroxyapatite surface by coating zwitterionic MPC polymer containing calcium-binding moieties to prevent oral bacterial adhesion. doi:10.1016/j.actbio.2016.03.006
Acta Biomaterialia Bacterial suspension Streptococcus mutans
Fluorescent Immuno-histochemical stainingAnalysis of anti-biofouling surfaces
LSM 880
Publication Journal Sample Application System
2016. A. Koch et al. An RNAi-Based Control of Fusariumgraminearum Infections Through Spraying ofLong dsRNAs Involves a Plant Passage and isControlled by the Fungal Silencing Machinery. doi:10.1371/journal.ppat.1005901
PLOS, Pathogens Hordeum vulgare (Barley)Fusarium graminearum strain Fg-IFA65
In vivo imagingSpectral / Lambda imaging (Linear unmixing, Online fingerprinting)
LSM 880
2016. E. Breeze et al. A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon. doi:10.1073/pnas.1605434113
PNAS Arabidopsis thaliana In vivo: ER network analysisFRAP
LSM 880Airyscan
2016. O. Sztatelman et al. Fine tuning chloroplast movements through physical interactions between phototropins. doi:10.1093/jxb/erw265
Journal of Experimental Botany
Arabidopsis thaliana Live imagingChloroplast movement
LSM 880
2016. M. R. Greaney et al. Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio. doi:10.1002/cne.24042
Journal of Comparative Neurology
Zebrafish Live cell imagingIn vivo imaging, mCherry
LSM 800
2016, H. L. Johnsen and H. R. Horvitz. Both the apop-totic suicide pathway and phagocytosis are required for a programmed cell death in Caenorhabditis elegans. doi:10.1186/s12915-016-0262-5
BMC Biology Caenorhabditis elegans Live cell imaging LSM 800
2016, J. Firmino et al. Cell Division Drives Epithelial Cell Rearrangements during Gastrulation in Chick. doi:10.1016/j.devcel.2016.01.007
Developmental Cell Chicken embryo Live imaging of gastrulation
LSM 700, 880LSM 7 MP
Cell culture / In vitro / Fixed cells
Whole organisms / In vivo imaging
Application Note
7
Publication Journal Sample Application System
2016. Y. Yoshimura et al. Roles of 5-HT1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons. doi:10.1016/j.neures.2016.09.008
Neuroscience Research Mouse Fluorescent Immuno-histochemical staining
LSM 800Airyscan
2016. F. Mora-Bermúdez et al. Differences and similari-ties between human and chimpanzee neural progenitors during cerebral cortex development. doi:10.7554/eLife.18683
eLIFE Cerebral organoids Fluorescent Immuno-histochemical staining
LSM 880 Airyscan
2016. Y. Shinohara et al. Viral Vector-Based Dissection of Marmoset GFAP Promoter in Mouse and Marmoset Brains. doi:10.1371/journal.pone.0162023
PLOS, ONE MiceMarmosetBrain tissue sections
Fluorescent Immuno-histochemical staining
LSM 880
2016. T.-M. Fu et al. Stable long-term chronic brain map-ping at the single-neuron level. doi:10.1038/nmeth.3969
Nature methods Mouse Fluorescent Immuno-histochemical staining
LSM 880
2016. M. J. Costello et al. Identification and Ultrastruc-tural Characterization of a Novel Nuclear Degradation Complex in Differentiating Lens Fiber Cells. doi:10.1371/journal.pone.0160785
PLOS, ONE Chicken embryosEye lenses
Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016. S.J. Miller and J.D. Rothstein. Astroglia in thick tissue with super resolution and cellular reconstruction. doi:10.1371/journal.pone.0160391
PLOS, ONE Mouse, BAC-GLT1-eGFP and 8.3kb-tdTomato
Clearing, passive ClarityFluorescent Immuno-histochemical staining
LSM 800 Airyscan
2016. T.G. Mc Williams et al. mito-QC illuminates mito-phagy and mitochondrial architecture in vivo. doi:10.1083/jcb.201603039
JBCThe Journal of Cell Biology
Mouse (Brain, Kidney)mito-QC MEFsTissue sections
Fluorescent Immuno-histochemical stainingFluorescent proteinsLive cell imaging
LSM 880Airyscan
2016. C. Dhang et al. Bio-Inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering. doi:10.1016/j.biomaterials.2016.07.007
Biomaterials Collagen scaffold for bone tissue
LSM 800Airyscan
2016. H. Ren et al. SPIKE1 activates ROP GTPase to modulate petal anisotropic growth in Arabidopsis. doi:10. 1104/ pp. 16. 00788
Plant Physiology Arabidopsis thaliana LSM 880
2016, N. Lehotai et al. Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis. doi:10.1007/s00299-016-2028-5
Plant Cell Reports Arabidopsis thaliana LSM 880
2016, A. Sherlekar and R. Rikhy. Syndapin promotes pseudocleavage furrow formation by actin organization in the syncytial Drosophila embryo. doi:10.1091/mbc.E15-09-0656
MBoCMolecular Biology of the Cell
Drosophila melanogster Fluorescent Immuno-histochemical staining
LSM 800 Airyscan
2016, K. N. Richter et al. Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane. doi:10.1007/s00441-016-2423-9
Cell and tissue Research Apis mellifera (honeybee)Hypopharyngeal gland
Fluorescent Immuno-histochemical staining
LSM 880 Airyscan
2016, S. J. Terrill et al. Role of Lateral Septum Glucagon-Like Peptide 1 Receptors in Food Intake. doi:10.1152/ajpregu.00460.2015
American Physiological Society
LSM 880 NLO
2016, S.-N. Wang et al. Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae). doi:10.1016/j.jinsphys.2016.05.002
Journal of Insect Physiology
Microplitis mediator (Hymenoptera, Braconidae)Antennae
Fluorescent Immuno-histochemical staining
LSM 880
2016, H. Li et al. ROCK inhibitor abolishes the antibody response in experimental autoimmune myasthenia gravis. doi:10.1016/j.mcn.2016.05.001
Molecular and Cellular Neuroscience
RatGastrocnemius muscle tissue
Fluorescent Immuno-histochemical staining
LSM 800
2016, P. Sandeep et al. Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration. doi:10.1242/dev.131292
Development Zebrafish Fluorescent Immuno-histo-chemical stainingFluorescent in situ Hybridisation
LSM 800
2016, S. Lei et al. Increased and prolonged human noro-virus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency. doi:10.1038/srep25222
NatureScientific Reports
Intestinal tissue sections from RAG2/IL2RG deficient pigs (CRISPR/Cas9)
Fluorescent Immuno-histochemical staining
LSM 880
Organ, tissue, whole organisms
Application Note
8
Publication Journal Sample Application System
2016, K. Kolahi et al. Real-time tracking of Bodipy-C12 long-chain fatty acid in human term placenta reveals unique lipid dynamics in cytotrophoblast cells. doi:10.1371/journal.pone.0153522
PLOS, ONE Human placenta explants Fluorescent Immuno-histochemical staining
LSM 880Airyscan
2016, S. Lei et al. Enterobacter cloacae inhibits human norovirus infectivity in gnotobiotic pigs. doi:10.1038/srep25017
Nature Scientific Reports
Pig, small intestinal tissue Fluorescent Immuno-histochemical staining
LSM 880
2016, Y. Leonhardt et al. Functional characterization of the Woronin body protein WscA of the pathogenic mold Aspergillus fumigatus. doi:10.1016/j.ijmm.2016.03.008
Internal Journal of Medical microbiology
Aspergillus fumigatus Fluorescent Immuno-histochemical staining
LSM 880 Airyscan
2016, K. A. Halberg et al. The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. doi:10.1038/ncomms11266
Nature Communications
Drosophila melanogaster Fluorescent Immuno-histochemical staining
LSM 880 Airyscan
K. Meng-Tsen et al. Super-Resolution Mapping of Neu-ronal Circuitry With an Index-Optimized Clearing Agent. doi:10.1016/j.celrep.2016.02.057
Cell Reports Mouse (brain, oocyte)Drosophila melanogasterHEK293T cells
Water based clearing, SeeDBFluorescent proteinsFluorescent Immuno-histo-chemical staining
LSM 880 Airyscan
2016, I. M. Berke et al. Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing. doi:10.1371/journal.pone.0150268
PLOS, ONE Mouse Skeletal muscle tissue
Clearing (water-based) Comparison of clearing methods Fluorescent Immuno-histochemical staining
LSM 880LSM 780 NLO
2015, P.P. de Abreu Manso et al. Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. doi:10.1371/journal.pntd.0004064
PLOS, Neglected Tropical Diseases
Immunology Chicken embryos
Histological sections Fluorescent Immuno- histochemical staining Assay
LSM 710LSM 880AiryscanELYRA SR-SIM
Publication Journal Sample Application System
2016. J. Kang et al. A solvent depend on ratiometric flu-orescent probe for hypochlorous acid and its application in living cells. doi:10.1016/j.dyepig.2016.09.048
Dyes and Pigments A549 cell culture Fluorescent sensor Live cell imaging
LSM 880 Airyscan
2016. J. Miao et al. A new class of fast-response and high-ly selective fluorescent probes for visualizing peroxynitrite in live cells, subcellular organelles, and kidney tissue of diabetic rats. doi:10.1016/j.biomaterials.2016.08.032
Biomaterials Rat Live cells Tissue
LSM 880 Airyscan
2016. G. Follain et al. Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology. doi:10.1242/jcs.189001
Journal of Cell Science C. elegans Zebrafish Mouse
Comparisons of systems for in vivo imaging
Airyscan
2016. M. Sivaguru et al. Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen. doi:10.1002/jemt.22732
Microscopy Research and Technique
Pollen Unlabeled and labelled pollen Comparison of Confocal, Airyscan, and SR-SIM
LSM 880 Airyscan
2016, Y. Zhao et al. A new far-red naphthorhodamine dye: Synthesis, fluorescent probe and bioimaging appli-cations. doi:10.1016/j.dyepig.2016.05.012
Dyes and Pigments HeLa cell culture Far-red naphthorhodamine dye Labelling mitochondriaLive cell imaging
LSM 880
2016, M. A. Urban eta al. Cuticle and subsurface orna-mentation of intact plant leaf epidermis under confocal and superresolution microscopy. doi:10.1002/jemt.22667
Microscopy Research and Technique
Ginko biloba, intact plant leafs Clearing, embedding media analyzed for con-focal and superresolution microscopy
LSM 880 Airyscan
2016, J. Chao et al. A single fluorescent probe for multiple analyte sensing: efficient and selective detection of CN−, HSO3− and extremely alkaline pH. doi:10.1039/C6TB00119J
Materials for biology and medicine
E. coli cells IECBT Detection of analytes including CN-, HSO3-, alkaline pH
LSM 880
Organ, tissue, whole organisms
Imaging Methods
Carl Zeiss Microscopy GmbH 07745 Jena, Germany [email protected] www.zeiss.com/microscopy
EN_4
1_01
3_13
3 | C
Z 12
-201
6 | D
esig
n, s
cope
of
deliv
ery
and
tech
nica
l pro
gres
s su
bjec
t to
cha
nge
with
out
notic
e. |
© C
arl Z
eiss
Mic
rosc
opy
Gm
bH
Not
for
the
rape
utic
, tre
atm
ent
or m
edic
al d
iagn
ostic
evi
denc
e. N
ot a
ll pr
oduc
ts a
re a
vaila
ble
in e
very
cou
ntry
. Con
tact
you
r lo
cal Z
EISS
rep
rese
ntat
ive
for
mor
e in
form
atio
n.
Copyright Notice (Cover image):
upper left: Sample courtesy of Michael W. Davidson, The Florida State University
upper right: Sample: courtesy of T. Pasternak, Institute of Biology, Albert Ludwigs University Freiburg, Germany
bottom left: Dr. Jan Michels, GEOMAR Helmholtz Centre for Ocean Research Kiel and Zoological Institute, Kiel University
bottom right: Courtesy of Dr. Julia Sellin, AG Hoch, LIMES Institut, Bonn