Zebrafish Embryo Dispenser - ZED Gunnlaugur Steinar Guðmundsson Óli Sævar Ólafsson Rúnar Viggósson Örnólfur Þórir Örnólfsson
Jan 19, 2018
Zebrafish Embryo Dispenser - ZED
Gunnlaugur Steinar GuðmundssonÓli Sævar ÓlafssonRúnar Viggósson
Örnólfur Þórir Örnólfsson
What is an Zebra fish embrio and what do you do with them
The new lab rats (the test rats)• Used in different stages,
3Z begin the testing when 4-7 days old
• 3Z uses the embryos to research sleeping behavior.
• Reaction of a whole organism
• The biggest advantage is they breed very quickly
http://commons.wikimedia.org/wiki/File%3AZebrafish_embryos.png
http://www.freakingnews.com/Zebrafish-Pics-43457.asp
Functional requirements• Get the embryos from the
container into something more suiteble
• Line the embryos up for splitting• Split the embryos up• Detect an embryo or no embryo• Dispense a single embryo to the
wellplate• 350 µl/well• No is better than two
•http://www.nanopartz.com/Inspiration.htm
The problem has been solved before by Union Biometica
But with some drawbacks for our mission:• Works only good for
the embryos before they can move.
• Comes with a lot of devices we don´t need.
• Costs about $300.000 before shipping and taxes
http://www.unionbio.com/copas/
Design parameters (ideas)
• Use a spiral pump (must be fed)• Use a syringe and a small tube• Put the embryos on a shrimp grader• Use a break beam sensor• Use a delta robot to pick the embryos up and
put in the wellplate
Experiments on concepts
• Picking up embryos
Experiments on concepts
• Embryos in a syringe
Design parameters (final idea)
• Pour manually to the container• Use a syringe and a small tube• Use a syringe (or a pump), connect it to the tube
from the syringe and flush out the embryo if needed
• Use a break beam sensor• Use the 3D printer at the end of the tube to move
the wellplate• Valve somewhere on the way to divert the flow
Experiment on concepts
• Bracket for sensor
Experiments on concepts
• Break beam sensor
Final design
3D printer
To summarize
• Challanging problem– Hard because of the size of the fishes
• $1,174 vs $300,000