This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
科学研究費助成事業 研究成果報告書
様 式 C-19、F-19、Z-19 (共通)
機関番号:
研究種目:
課題番号:
研究課題名(和文)
研究代表者
研究課題名(英文)
交付決定額(研究期間全体):(直接経費)
13901
挑戦的萌芽研究
2014~2013
抗体結合型バイオナノカプセルを応用した炎症白血球特異的新規腎障害治療法の開発
Development of novel therapy for kidney injury by leukocyte targeting antibody-fused Bionanocapsules
研究成果の概要(英文):In this study, we aimed the development of evolutional therapy to reconcile both anti-inflammatory/immune suppressive effects and extenuate adverse effects. We applied bionanocapsule-based drug delivery system (DDS) to inhibit immune activation specific for macrophages and selectively at inflammatory sites. We generated fluorescent-conjugated BNC fused with antibody targeting macrophages, and confirmed the BNC distribution on mouse macrophage cell line. Intravenous administration of the BNC into mouse demonstrated fluorescent cells only on injured kidney indicating specific BNC delivery for inflammatory sites. Significant expression of gene enclosed in BNC was not demonstrated, but this study provided fundamental data for the development for leukocyte specific DDS using BNC.
1. Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S. Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy. Clin Exp Nephrol. 2014 Nov 21.Epub 2. Sato Y, Sato W, Maruyama S, Wilcox CS, Falck JR, Masuda T, Kosugi T, Kojima H, Maeda K, Furuhashi K, Ando M, Imai E, Matsuo S, Kadomatsu K. Midkine Regulates BP through Cytochrome P450-Derived Eicosanoids. J Am Soc Nephrol. 2014 Nov 5. Epub 3. Abe T, Kato S, Tsuruta Y, Sugiura S, Katsuno T, Kosugi T, Tsuboi N, MatsuoS,MaruyamaS.Neutrophil/lymphocyte ratio as a predictor of cardiovascular events in incident dialysis patients: a Japanese prospective cohort study. Clin Exp Nephrol. 2014 Oct 28. Epub 4. Yamaguchi M, Ando M, Yamamoto R, Akiyama S, Kato S, Katsuno T, Kosugi T, Sato W, Tsuboi N, Yasuda Y, Mizuno M, Ito Y, Matsuo S, Maruyama S. Patient age and the prognosis of idiopathic membranous nephropathy. PLoS One. 2014 Oct 20;9(10):e110376. 5. Shi Y, Tsuboi N, Furuhashi K, Du Q, Horinouchi A, Maeda K, Kosugi T, Matsuo S, Maruyama S. Pristane-induced granulocyte recruitment promotes phenotypic conversion of macrophages and protects against diffuse pulmonary hemorrhage in Mac-1 deficiency. J Immunol. 2014 Nov 15;193(10):5129-39. 6. Yamaguchi M, Ando M, Yamamoto R, Akiyama S, Kato S, Katsuno T, Kosugi
T, Sato W, Tsuboi N, Yasuda Y, Mizuno M, Ito Y, Matsuo S, Maruyama S. Smoking is a risk factor for the progression of idiopathic membranous nephropathy. PLoS One. 2014 Jun 25;9(6):e100835. 7. Yamaguchi M, Yoshioka T, Yamakawa T, Maeda M, Shimizu H, Fujita Y, Maruyama S, Ito Y, Matsuo S. Anti-neutrophil cytoplasmic antibody-associated vasculitis associated with infectious mononucleosis due to primary Epstein-Barr virus infection: report of three cases. Clin Kidney J. 2014 Feb;7(1):45-8. 8. Kim H, Mizuno M, Furuhashi K, Katsuno T, Ozaki T, Yasuda K, Tsuboi N, Sato W, Suzuki Y, Matsuo S, Ito Y, Maruyama S. Rat adipose tissue-derived stem cells attenuate peritoneal injuries in rat zymosan-induced peritonitis accompanied by complement activation. Cytotherapy. 2014 Mar;16(3):357-68. 9. Ito Y, Mizuno M, Suzuki Y, Tamai H, Hiramatsu T, Ohashi H, Ito I, Kasuga H, Horie M, Maruyama S, Yuzawa Y, Matsubara T, Matsuo S; Nagoya Spiro Study Group. Long-term effects of spironolactone in peritoneal dialysis patients. J Am Soc Nephrol. 2014 May;25(5):1094-102. 10. Nagaya H, Kosugi T, Maeda-Hori M, Maeda K, Sato Y, Kojima H, Hayashi H, Kato N, Ishimoto T, Sato W, Yuzawa Y, Matsuo S, Kadomatsu K, Maruyama S. CD147/basigin reflects renal dysfunction in patients with acute kidney injury. Clin Exp Nephrol. 2014 Oct;18(5):746-54. 11. Shinjo H, Sato W, Imai E, Kosugi T, Hayashi H, Nishimura K, Nishiwaki K, Yuzawa Y, Matsuo S, Maruyama S. Comparison of kidney disease: improving global outcomes and acute kidney injury network criteria for assessing patients in intensive care units. Clin Exp Nephrol. 2014 Oct;18(5):737-45. 12. Tsuboi N, Maruyama S, Matsuo S, Imai E. A ray of light in the dark: alternative approaches to the assessment and treatment of ischemic nephropathy. Nephrol Dial Transplant. 2014 Feb;29(2):228-31. 13. Kinashi H, Ito Y, Mizuno M, Suzuki Y, Terabayashi T, Nagura F, Hattori R, Matsukawa Y, Mizuno T, Noda Y, Nishimura H, Nishio R, Maruyama S, Imai E, Matsuo S, Takei Y. TGF-β1
promotes lymphangiogenesis during peritoneal fibrosis. J Am Soc Nephrol. 2013 Oct;24(10):1627-42. 14. Mizuno M, Suzuki Y, Nonaka K, Sei Y, Maruyama S, Matsuo S, Ito Y. Perforative peritonitis caused by appendicitis in a patient on peritoneal dialysis. Intern Med. 2013;52(11):1177-81. 15. Furuhashi K, Tsuboi N, Shimizu A, Katsuno T, Kim H, Saka Y, Ozaki T, Sado Y, Imai E, Matsuo S, Maruyama S. Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages. J Am Soc Nephrol. 2013 Mar;24(4):587-603. 16. Kojima H, Kosugi T, Sato W, Sato Y, Maeda K, Kato N, Kato K, Inaba S, Ishimoto T, Tsuboi N, Matsuo S, Maruyama S, Yuzawa Y, Kadomatsu K. Deficiency of growth factor midkine exacerbates necrotizing glomerular injuries in progressive glomerulonephritis. Am J Pathol. 2013 Feb;182(2):410-9. 17. Yamamoto J, Sato W, Kosugi T, Yamamoto T, Kimura T, Taniguchi S, Kojima H, Maruyama S, Imai E, Matsuo S, Yuzawa Y, Niki I. Distribution of hydrogen sulfide (H ₂S)-producing enzymes and the roles of the H₂S donor sodium hydrosulfide in diabetic nephropathy. Clin Exp Nephrol. 2013 Feb;17(1):32-40.
〔学会発表〕(計 13 件) 1. DuQiuna、坪井直毅、石一沁、松尾清一、
丸山彰一. 第 57 回日本腎臓学会学術総会.パシフィコ横浜.神奈川県横浜市.2014/7/4
2. N. Endo, N. Tsuboi, K. Furuhashi, S. Matsuo, S. Maruyama. ASSOCIATION OF GLOMERULAR MACROPHAGE PHENOTYPES AND URINE SOLUBLE CD163 WITH DISEASE ACTIVITY IN HUMAN LUPUS NEPHRITIS. 9th International Congress on Autoimmunity. Nice.France.2014/3/29
3. Nobuhide Endo, DrMed, Naotake Tsuboi, MD, PhD, Kazuhiro Furuhashi, MD, PhD, Shoichi Maruyama, MD, Seiichi Matsuo. Association of Glomerular Macrophage Phenotypes and Urinary Soluble CD163 with Disease Activity in Human Lupus Nephritis. Kidney Week 2014. フィラデルフィア.アメリカ.2014/11/15
7. 石一沁,坪井直毅,古橋 和拡,Mayadas Tanya,丸山 彰一,松尾清一. Mac-1 deficiency protects mouse from pulmonary hemorrhage, whereas exacerbates glomerulonephritis in experimental model of systemic lupus erythematosus. 第34回日本炎症再生医学会. 京都市左京区.国立京都国際会館. 2013/7/3
8. Tomoko Abe, Asuka Horinouchi, Hangsoo Kim, Kazuhiro Huruhashi, Shinichi Aiyama, Takayuki Katsuno, Kaoru Yasuda, Takinori Ozaki, Naotake Tsuboi, Seiichi Matsuo, Shouichi Maruyama. LOW SERUM CULTURED ADIPOSE-DERIVED MESENCHIMAL STROMAL CELLS AMERIORATE RAT MODEL WITH UNILATERAL URETERAL OBSTRUCTION. The World Congress of Nephrology 2013. Hong Kong.2013/6/1
9. KazuhiroFuruhashi,NaotakeTsuboi,AsukaShimizu,HangsooKim,TakayukiKatsuno,Seiichi Matsuo, Shoichi Maruyama. SERUM-STARVED ADIPOSE-DERIVED STROMAL CELLS AMELIORATE RENAL INJURY ASSOCIATED WITH RAT CRESCENTIC GLOMERULONEPHRITIS BY PROMOTING THE GENERATION OF IMMUNOREGULATORY MACROPHAGES. The World Congress of Nephrology 2013. Hong Kong.2013/6/1
10. Hangsoo Kim,Masashi Mizuno, Kazuhiro Furuhashi,TakayukiKatsuno,Takenori Ozaki,KaoruYasuda,NaotakeTsuboi,YasuhikoIto,ShoichiMaruyama,MatsuoSeiichi. RAT ADIPOSE TISSUE-DERIVED STROMAL CELLS ATTENUATE PERITONEAL INJURIES IN RAT ZYMOSAN-INDUCED PERITONITIS ACCOMPANIED BY COMPLEMENT ACTIVATION. The World Congress of Nephrology 2013. Hong Kong.2013/6/1