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RPCV (2013) 108 (585-586) 69-91
XVIII Meeting of the Portuguese Society of Animal Pathology -
International Meeting of Leporidae Pathology
Editorial
The Portuguese Society of Animal Pathology (SPPA) is a small
non-profit organization and a daughter So-ciety of the Portuguese
Society of Veterinary Science (SPCV), which normally holds an
annual meeting in the institutions from which its members belong
(e.g. universities or government laboratories with activities in
the field of Pathology).
In an effort to decentralize their meetings, a tradition already
started before, and both to aim at involving the community of
teachers and students of the research in-stitutions that host the
annual meeting and also to di-versify the main subject of its
meetings, SPPA targeted new, younger and broader audiences, as well
as new sectors and knowledge fields, trying to cover the pa-thology
of a wide animal species range. Hence, to the present meeting we
have chosen leporideae pathology as the main subject and a necropsy
workshop, open to students and technicians - XVIII Meeting of the
Por-tuguese Society of Animal Pathology. International Meeting of
Leporidae Pathology, 9 and 10th of May 2013.
Also, continuing the journey, already begun, of aiming evermore
towards internationalization, this meeting will have reputable
national and internation-al speakers, to whom above all I
acknowledge their presence and immediate acceptance. Moreover, the
scientific committee with national and international members, the
three official languages, and the wid-est dissemination among
technicians and students, will certainly make this meeting more
open, more in-volved and more attractive. So we hope, so we wish,
so we worked for!
Our special guests will include an international ex-pert, who
will share with us his scientific knowledge, Prof. Dr. Daniel
Fernandez de Luco Martinez, profes-sor at the University of
Zaragoza in the Department of Animal Pathology, Faculty of
Veterinary Science. Graduated in Veterinary Medicine by the
University of Zaragoza in 1985 and obtained a PhD by the same
institution in 1991. He has developed his research in pathology of
wildlife and wildlife hunting health con-trol in the region of
Aragon in Spain. He has dozens of publications in international
peer-review journals and was a guest speaker at dozens of
conferences. He is responsible for several research projects,
having juried several doctoral theses.
Also, not forgetting nationals with high value and enriching
practical experience in the field, an Europe-an expert, Dr. Jos
Manuel Monteiro Azevedo. He has earned his degree in Veterinary
Medicine in the Uni-versity of Tras-os-Montes and Alto Douro in
1999, and is currently a Veterinarian for Coren, Spain, being the
Head of Industrial Rabbit Farming and the responsible for the
Industrial Cuniculture in the Iberian Peninsula. His professional
activity, since graduation, has been dedicated to rabbits having
taken several positions at national and international companies. He
collaborated in teaching several courses at the University of
Trs-os-Montes and Alto Douro.
We will have the opportunity to share and exchange experiences
on animal pathology, results of research projects, discuss
pathological diagnosis of difficult or rare cases, as well as the
recent scientific advances in this field in Europe, which is
undoubtedly the largest professional enrichment we could obtain.
For the 18th time this small scale but dynamic meeting, constitutes
an enrichment both scientifically, technically and pro-fessionally,
and will allow us to grow a bit more pro-fessionally. Moreover,
this will be also an excellent op-portunity to meet again our
colleagues and friends and to discover Evora, one of the most
interesting, beauti-ful and pleasant cities to visit in
Portugal.
The success of this meeting is already notorious by the number
of members who decided to join us. Thank you for believing in this
project, thank you for being here!
In the name of SPPA and the Organizing Commit-tee, I want to
acknowledge all those who contributed to make this event possible,
namely the University of vora, in the person of its Rector Ex Sr.
Prof. Dr. Carlos Alberto dos Santos Braumann and of its
Vice-Rector, Prof. Dr. Marta Silvrio, for immediately wel-coming
this endeavor, namely for all the logistical sup-port provided and
specially the generous availability in providing their facilities.
I would like to acknowledge the Portuguese Society of Veterinary
Sciences who provided an important assistance in the financial
man-agement, and all those who helped us, teachers, stu-dents,
staff and sponsors.
The President of SPPA
Helena Maria Vala Correia
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Common gross lesions in farmed rabbits
Fernndez de Luco D. *
Departamento de Patologa Animal. Facultad de Vete-rinaria.
Universidad de Zaragoza. C/ Miguel Servet 177. 50013 Zaragoza.
Spain.
* [email protected]
Introduction: The identification of rabbit gross le-sions by
systems and organs in association with a dis-ease, syndrome,
aetiology or unknown cause is very important for veterinary
practitioners.
Material and methods: Necropsy has been made in several farmed
rabbits with mainly respiratory or di-gestive problems, as well as
delayed growth.
Results: Dermatitis associated to myxomatosis, ringworm, mange
or staphylococci are common.
The respiratory system problems are localized in the nasal
cavity and lungs mainly associated to Pasteurella multocida and
Bordetella bronchiseptica. Young rab-bits are especially sensitive
to acute and chronic pneu-monia. Haemorrhages can be seen in cases
of rabbit calicivirus disease.
Probably, digestive processes are the most important problems in
growing rabbits. Epizootic rabbit enterop-athy, with still unknown
aetiology, is a relatively recent process that has become the main
cause of mortality in farmed rabbit. Other enteritis processes are
princi-pally associated to coccidia, E. coli, Clostridium spp., C.
spiroforme, C. piliforme (Tyzzers disease), Salmo-nella spp., as
well as nematodes (Passalurus spp.). In-flammation and necrosis of
the liver can be observed as small white spots associated to
Eimeria stiedae (hepatic coccidiosis), Salmonella spp., C.
piliforme, Cysticercus pisiformis, etc. Necrosis with karyorrhexis
of the hepatocytes is a common finding in rabbit cali-civirus
disease.
Testis, uterus and mammary gland are the main or-gans affected
in the reproductive system, as well as dystocia in pregnant
rabbits. Processes and agents mainly involved are mixomatosis,
syphilis (Treponema cuniculi), P. multocida, Staphylococcus spp.,
etc
Finally, encephalitozoonosis by Encephalitozoon cuniculi is
present in many rabbitries. Nervous signs, granulomatous
encephalitis and interstitial nephritis are present in the affected
animals.
Situaes de urgncia em cunicultura industrial
Monteiro J.M.1*, Alegria N.2, Coelho A.C.2
1 Sumicor Portugal Lda; Coren S. C. G.2 Dept. de Cincias
Veterinrias, CECAV, Universidade
de Trs-os-Montes e Alto Douro (UTAD)
* [email protected]
Introduo: O mdico veterinrio durante a sua atividade contribui
para a reduo das perdas associa-das morbilidade e mortalidade em
cunicultura, para a melhoria dos resultados produtivos, atuando
como consultor e orientando o produtor nos investimentos.
O presente trabalho teve como objetivo reportar a atuao do mdico
veterinrio na sua vertente clnica, dando nfase s principais situaes
de emergncia em cunicultura.
Material e mtodos: Procedeu-se realizao de um estudo
observacional retrospetivo da actividade clnica na Pennsula Ibrica,
durante os anos de 2009 a 2012.
Foi efectuado o exame clnico dos animais prove-nientes de 76
exploraes, tendo-se procedido rea-lizao de necrpsias, com recurso a
exames laborato-riais e elaborao dos respetivos registos que
serviram de base ao presente estudo.
Resultados: Determinou-se que a maioria das cha-madas de
urgncia, se deveram s condies clnicas de enteropatia mucide,
colibacilose, pasteurelose, po-dodermatite, mixomatose e
enterotoxemia cujas leses anatomopatolgicas foram documentadas.
As exploraes com maternidade e engorda no mes-mo pavilho, mau
maneio, sem controlo ambiental e sujeitas a variaes climticas,
situaes potencialmen-te geradoras de maior stresse e consequente
reduo da imunidade, apresentaram aumento da morbilidade e menor
produtividade, enquanto as exploraes com ambiente controlado e bom
maneio, revelaram menos situaes de emergncia clnica e boa
produtividade.
Concluso: Foi possvel verificar que as exploraes onde as medidas
de bem-estar animal e de controlo am-biental rigoroso j se
encontravam implementadas, apre-sentaram melhores resultados
produtivos e menor mor-bilidade em todas as fases de produo. Os
resultados sugerem que a actuao do mdico veterinrio dever in-cidir
tambm nas vertentes atrs referidas, pois s assim se conseguir
aumentar a rentabilidade das exploraes.
Emergency Situations in Industrial Rabbit Farming
Introduction: The veterinary doctor in the course of his
activities contributes to the reduction of losses as-
Plenary sessions
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SUPLEMENTO RPCV (2013) 108 (585-586) 69-91
71
sociated with morbidity and mortality, by improving the results
of production, acting as a consultant, and orienting the producer
in his investments.
The present paper has as its objective to report on the
performance of the veterinary doctor in the clini-cal aspect of his
work, giving emphasis to the princi-pal emergency situations in
rabbit farming.
Material and methods: A retrospective observa-tional study was
carried out of the clinical activity in the Iberian Peninsula
during the years 2009 to 2012.
The clinical examination of animals from 76 inves-tigations was
carried out, subsequent to the realization of autopsies, with the
use of laboratory tests whenever necessary and the elaboration of
the respective regis-ters which served as a base for the present
study.
Results: It was found that the majority of emergency calls,
resulted from the clinical conditions of mucoid enteropathy,
colibacillosis, pasteurellosis, foot pad der-matitis, myxomatosis
and enterotoxaemia, of which the
anatomopathological injuries were documented.Farms with
maternity and fattening stages in the same
building, poor handling, without environmental control and
subjected to climatic variations, all of which were potential
generators of major stress and consequently reduction in immunity,
showed an increase in morbidity and lower productivity, while the
farms with environ-mental control and good handling revealed less
clinical emergency situations and good productivity.
Conclusion: It was possible to verify that the farms where the
animal welfare measures being and rigor-ous environmental control
had already been imple-mented showed better results in production
and less morbidity in all the phases of production. The re-sults
suggest that the activity of the veterinary doctor should also
occur in the aspects previously referred; as it is only in this way
that it will be possible to in-crease the profitability of the
farms.
Controlo de pragas em parques Zoolgicos: repercusses
parasitrias, patolgicas e de Sade Pblica
Crespo, A.P.1,2*, Lapo, N.1, Correia, J. 2, Gomes, L. 2, Vaz,
Y.2 Madeira de Carvalho, L.M.2
1 Jardim Zoolgico de Lisboa, Estrada de Benfica, 158-160,
1549-004 Lisboa;
2 CIISA, Faculdade de Medicina Veterinria, Universi-dade Tcnica
de Lisboa, Av. da Universidade Tcnica de Lisboa, 1300-477 Lisboa,
Portugal ([email protected]);
* [email protected]
Introduo: A ligao prxima entre animais, Homem e pragas em zoos
ou parques zoolgicos, constitui um risco na transmisso de muitos
agentes de doena, alguns dos quais ocasionando doenas zoonticas,
podendo afectar no s os animais da coleco zoolgica, como tambm
trabalhadores do zoo e visitantes.
Material e mtodos: Integrado num estudo so-bre controlo de
pragas no Jardim Zoolgico de Lis-boa, efectuou-se um rastreio
parasitolgico em 100 roedores (50 Mus musculus e 50 Rattus
norvegicus). Os endoparasitas adultos foram coligidos aps
de-cantaes dos contedos gastrintestinais e esfregaos por aposio do
fgado (colorao pelo Giemsa) e de fezes (colorao pelo
Ziehl-Neelsen), efectuando-se anlises coprolgicas por flutuao
(mtodo de Wil-lis) e de sedimentao espontnea. Os fgados foram
submetidos a anlise histolgica, fragmentos do f-
gado foram fixados durante 24 horas, procedeu-se ao corte dos
blocos de parafina e as amostras foram fixadas e coradas pela
colorao da Hematoxilina e Eritrosina.
Resultados: Dos roedores observados, 82 apresen-taram formas de
endoparasitas, com prevalncias de 84,0% para R. norvegicus e de
80,0% para M. muscu-lus. Das espcies de roedores, Rattus norvegicus
rep-resentou maior importncia na transmisso dos para-sitas
assinalados. Identificaram-se um total de cinco espcies de
endoparasitas com potencial zoontico: Hymenolepis diminuta (16,0%),
Cysticercus fasciola-ris (12,0%), Syphacia obvelata (37,0%),
Calodium hepaticum (32,0%) e Cryptosporidium sp. (5,0%).
Concluso Deste conjunto de parasitas, C. hepati-cum, que j foi
detectado aps a morte de primatas no humanos com consequncias
patolgicas no f-gado daqueles hospedeiros. Alm da perda de
ani-mais, o risco zoontico de transmisso de parasitas, bactrias e
vrus aos trabalhadores e visitantes do Jardim Zoolgico de Lisboa,
deve ser sempre con-siderado como um problema de grande importncia
e impacto em Sade Pblica.
Pest control in Zoos: parasitic, pathologi-cal and Public Health
repercussions
Introduction: The close connection between ani-mals, humans and
pests, poses a risk for the transmis-sion of many agents, some of
them causing zoonotic diseases, affecting not only the animals in
the zoo-logical collection, as well as, zoo workers and visitors
themselves.
Oral communications
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Material and methods: Following a pest control study at the
Lisbon Zoo, a survey on endo and ectopar-asites was performed on
100 commensal rodents (50 Mus musculus and 50 Rattus norvegicus).
Endopara-sites were collected from the gastrointestinal contents by
a decanting process (adult specimens). Smears by liver apposition
(Giemsa staining) and by extension feces (Ziehl-Neelsen staining),
together with copro-logical exams by Willis flotation and
spontaneous sedi-mentation techniques, were performed. The livers
sub-jected to histological analysis of liver fragments were fixed
for 24 hours, proceeded to cut the paraffin blocks and the samples
were fixed and stained by Hematoxy-lin staining and
Erythrosine.
Results: From the total number of studied rodents, 82 were
positive for endoparasites, with prevalence rates of 84.0% for R.
norvegicus and 80.0% for M.
musculus. Concerning the role of rodent species in parasite
transmission, Rattus norvegicus represented the greater importance.
Five species of endoparasites with zoonotic potential were
identified: Hymenolepis diminuta (16.0%), Cysticercus fasciolaris
(12.0%), Syphacia obvelata (37.0%), Calodium hepaticum (32.0%) and
Cryptosporidium sp. (5.0%).
Conclusion: Of this set of parasites, C. hepaticum constitutes
an extreme example, since its presence was already detected after
death in non-human primates, with severe consequences for these
hosts liver. Beside the loss of animals, the risk of transmission
of zoonotic parasites, bacteria and viruses to workers and visitors
of the Lisbon Zoo should always be considered as a problem of great
importance and impact on Public Health.
Um caso de intoxico por cebola (Allium cepa) em bovinos
Monteiro M. 1*, Mendona P. 1, Carvalho P.1, Maria-no I.2
1 Laboratrio de Patologia/INIAV;2 Laboratrio Veterinrio de
Montemor-o-Novo
* [email protected]
Introduo: A anemia hemoltica resultante do consumo prolongado de
cebolas, Allium cepa, tem sido descrita em vrias espcies animais
nomeada-mente em ces, bovinos e caprinos; o princpio txico presente
na cebola o n-propil-dissulfito responsvel por alteraes da
hemoglobina.
Os Autores descrevem vrios casos de mortes em bovinos adultos
que eram alimentados com pastagem natural e recebiam um suplemento
de silagem de des-perdcio de tomate misturada com cebolas e
alhos.
Caso clnico: Os animais apresentavam anemia, hemoglobinria e em
alguns casos, ictercia.
Foram realizadas as necrpsias de dois animais; a abertura do
cadver revelou um intenso cheiro a ce-bolas; observaram-se alteraes
hepticas e renais.
Discusso: Microscopicamente havia extensas zo-nas de necrose
heptica centrolobular e necrose dos tubos renais simultaneamente
com acumulao dum material compatvel com hemoglobina.
Os exames efectuados em animais coabitantes, para diagnstico
diferencial com doenas que podem apre-sentar sinais clnicos
idnticos, resultaram negativos para leptospirose sendo detectada,
em apenas um ani-mal, uma pequena infeco de Theileria annulata.
Concluso: As leses microscpicas so sugestivas de uma anemia
hemoltica tendo a sua associao ao suplemento alimentar em causa,
permitido concluir
que se trata dum caso de intoxicao por cebolas; acresce-se ainda
que, aps ter sido retirado o referido suplemento e administrada uma
teraputica de supor-te, os animais que estavam doentes recuperaram
e no houve mais mortalidade.
A case of onion (Allium cepa) intoxication in cattle
Introduction: In domestic animals the prolonged ingestion of
onion (Allium cepa) has long been asso-ciated with the emergence of
hemolytic anemia. This toxicity is due to the presence of n-propil-
disulphide.
In this presentation the authors describe the occur-rence of
several cases of toxicity in a grazing bovine herd due to ingestion
of silage containing tomato, on-ion and garlic by-products.
Clinical case: The affected animals showed ane-mia,
haemoglobinuria and, occasionally, jaundice leading to death in
some severe cases.
Three animals were submitted to necropsy and the most prominent
feature was a marked onion odor. The kidneys were dark brown and
the liver slightly orange. Microscopically extensive areas of
centrolobular liver necrosis as well as renal tubular necrosis with
accu-mulation of hemoglobin-like material were seen.
Differential diagnostic testing excluded leptopiro-sis and
revealed one Theileria annulata infected ani-mal.
Conclusion: The symptoms and the lesions ob-served were strongly
suggestive of a haemolytic ane-mia. Taken together with the feed
supplementation these facts enabled us to conclude for onion
toxicity. Furthermore, after the discontinuation of the referred
silage supplement and upon implementation of sup-portive care, the
affected animals began to recover and no further deaths
occurred.
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Um caso raro de neoplasia retrobulbar
Vala H1,2*, Mega A1,2, Santos C1,2, Cruz R1,2, Nbrega C1,2,
Mesquita JR1,2, Esteves F1,2, Vieira F3, Ferraz A4
1Escola Superior Agrria de Viseu, Instituto Politcni-co de
Viseu, Portugal;2Centro de Estudos em Educao, Tecnologias e Sade,
Instituto Politcnico de Viseu, Portugal;3Histocit, Portugal;4Clnica
Veterinria Planeta Animal, Aveiro, Portugal.
* [email protected]
Introduo: Neoplasias envolvendo o nervo tico so raras. O
meningioma intraorbitrio, a neoplasia mais comum sediada no nervo
tico, constitui apenas 3% de todos os meningiomas em ces, razo pela
qual existem poucos estudos para permitir prever os seus sintomas e
comportamento biolgico.
Objetivos: Os autores descrevem e submetem dis-cusso, um caso
raro de meningioma retrobulbar, em particular para apreciao da
graduao histolgica e o prognstico.
Histria Clnica: Um Labrador Retriever de 10 anos de idade, foi
apresentado com queixas de edema e descarga purulenta do olho
direito. Apresentava exof-talmia, protuso da terceira plpebra,
quemose, lcera profunda da crnea e ausncia de reflexo pupilar luz,
no mesmo olho. O exame fsico, oftlmico e ecogr-fico permitiu
diagnosticar exoftalmia devido a massa retrobulbar.
Material e Mtodos: A amostra foi fixada em for-mol tamponado a
10 %, processada de acordo com o protocolo de rotina, tendo
igualmente sido realizados estudos imunohistoqumicos, usando o
mtodo do Complexo Estreptavidina-biotina-peroxidase.
Resultados: O exame macroscopico revelou uma massa cnica,
localizada na poro retrobulbar do ner-vo tico, adjacente poro
posterior do globo ocular, apresentando colorao esbranquiada,
aspeto homo-gneo, lobulado e consistncia firme.
O exame microscopico revelou clulas fusiformes, com ncleo
redondo a oval, cromatina em padro pon-tilhado, citoplasma
eosinoflico, moderado, organizadas em ninhos, em torno de
capilares, separados por feixes interlaados, com estroma muito
vascularizado e escasso.
Concluso: Os autores sugerem o diagnstico de meningiomado tipo
transicional, tendo em conta as ca-ratersticas histopatolgicas e os
resultados do estudo de imunohistoqumica.
Agradecimentos: FCT e CI&DETS (PEst-OE/CED/UI4016/2011)
A rare case of retrobulbar neoplasia
Introduction: Neoplasms involving the optic nerve are rare.
Intraorbital meningiomas, the most common tumour of the optic
nerve, represent only 3% of all meningiomas in dogs, so few data
exists to predict its symptoms and biologic behaviour.
Objectives: The authors describe and submit for discussion, a
rare case of retrobulbar meningioma, in particular for assessment
of histological grade and prognosis.
Medical History: A 10 year old Labrador Retriever male dog was
presented for complaints of swelling and purulent discharge of the
right eye. The animal had ex-ophthalmos, protrusion of the third
eyelid, chemosis, deep corneal ulceration and no pupillary light
reflex. Exophthalmos due to a retrobulbar mass was diagnosed after
physical, ophthalmic and ultrasound examination.
Material and Methods: The sample was fixed in 10 % buffered
formalin solution, processed for routine histopathological
diagnosis and a standard Streptavi-din-Biotin Complex technique for
immunohistochemi-cal evaluation was also performed.
Results: Macroscopic examination revealed a conic mass, located
in the retrobulbar portion of the optic nerve, adjacent to the
posterior aspect of the globe with homogeneous, lobulated and
whitish appearance and firm consistency.
Microscopic examination revealed spindled cells, with
round-to-oval, finely stippled nuclei, small or indistinct
nucleoli, moderate amount of eosinophilic cytoplasm, arranged in
concentric whorls around cap-illaries, separated by interlacing
fascicles, with small amount of well vascularized, collagenous
stroma.
Conclusion: The authors suggested the diagnosis of transitional
meningioma, based on the histopathologi-cal diagnosis and
immunohistochemistry results.
Acknowledgments: FCT and CI&DETS
(PEst-OE/CED/UI4016/2011)
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Ocorrncia de parasitismo externo em coelhos de companhia
Almeida, C.1*, Henriques C. 1, Bernardino, M. 1,2, Ma-deira de
Carvalho, L.M. 2
1 Exoclinic, R. Dom Antnio Ribeiro n1 loja B, 1495-145 Algs,
Portugal
2 CIISA, Fac Med Vet, Univ Tecn Lisboa, Av. da Univer-sidade
Tcnica de Lisboa, 1300-477 Lisboa, Portugal
* [email protected]
Introduo: Em Portugal, tal como sucedeu ante-riormente noutros
pases como Inglaterra, Estados Unidos e Alemanha, o coelho domstico
tem revelado crescente popularidade como animal de companhia,
devido sua grande inteligncia e interessante intera-o com a
famlia.
Durante o perodo de doze meses (Jan-Dez 2012) foram consultados
98 coelhos na Exoclinic sobre-tudo por problemas de
sobrecrescimento dentrio, problemas gastrointestinais e manifestaes
derma-tolgicas.
Material e mtodos: Dos 25 casos de manifestaes cutneas, para alm
do exame fsico foram submeti-dos a raspagem cutnea e citologia
direta das leses. Foram identificados 16 coelhos positivos a
parasitismo externo: Psoroptes cuniculi (69%) e Scarcoptes sca-biei
(31%). Dos animais afetados, 81% apresentavam menos de seis meses
de idade, verificando-se casos po-sitivos em machos e fmeas e nas
raas Fuzzy-Lop, Lion Head e Mini Holands.
O tratamento sugerido foi de injees subcutneas de ivermetina
(200g/kg) repetindo ao fim de 14 dias, 28 dias, sugerindo uma
quarta injeo e quinta injeo at desaparecimento total de sinais
clnicos. Em alter-nativa, utilizou-se em metade dos casos,
selametina so-luo tpica cutnea 15mg, repetindo-se ao fim de 28
dias, sugerindo uma terceira aplicao se necessrio.
Resultados: Em termos de desaparecimento das le-ses cutneas e
crescimento do plo nas zonas de alo-pcia os resultados obtidos
foram bem sucedidos ao fim de 28 dias de tratamento e com eficcia
equivalente com ambos os protocolos.
Em simultneo foram aplicadas medidas de melhor maneio e
higienizao das instalaes do animal, bem como o alerta do potencial
zoontico de alguns tipos de sarna. Ainda assim, verificou-se a
presena de dois episdios de manifestaes cutneas de sarna no
pro-prietrio, j instaladas aquando do incio tratamento do
animal.
Concluso: De acordo com a nossa experincia, o clnico deve estar
apto para a correta identificao dos agentes causais de problemas
dermatolgicos em coelhos. O aumento da casustica destes pacientes
re-presenta tambm novas perspetivas de diagnstico e
novos desafios teraputicos. na nossa opinio uma oportunidade
para instituir boas prticas veterinrias de preveno e profilaxia
destas doenas, pois o bom estado geral de sade est condicionado
pela correta informao e educao do proprietrio.
Occurrence of external parasitism in pet rabbits
Introduction: In Portugal, as happened previously in other
countries like England, Germany and the United States, the domestic
rabbit has shown increas-ing popularity as a pet, due to its great
intelligence and interesting interaction with the family.
During a twelve month period (Jan-Dec 2012) 98 rabbits were
consulted in Exoclinic, mainly due to dental problems,
gastrointestinal diseases and derma-tological manifestations.
Material and methods: Of 25 cases of cutaneous manifestations,
in addition to the physical examina-tion, they were subjected to
scrape and direct cytol-ogy of cutaneous lesions. We identified 16
rabbits positive for external parasitism: Psoroptes cuniculi (69%)
and Scarcoptes scabiei (31%). Of the affected animals, 81% were
less than six months old, with pos-itive cases in both males and
females and in Fuzzy-Lop, Lion Head and Mini Dutch breeds.
The suggested treatment was subcutaneous injec-tions of
ivermectin (200g/kg) repeating after 14 days and 28 days,
suggesting a fourth and a fifth injection until total disappearance
of clinical signs. Alterna-tively, in half the cases was used a
topicalolution of selamectin 15mg, repeating the treatment 28 days
af-ter the first one, suggesting a third application if
nec-essary.
Results: In terms of disappearance of skin lesions and hair
growth in areas of alopecia, results were suc-cessful after 28 days
of treatment, with equivalent ef-ficacy for both protocols.
Simultaneously were applied measures for a better handling and
hygiene of the animal facilities, as well as the alert for the
zoonotic potential of some types of mange. Still, there was the
presence of two episodes of cutaneous manifestations of scabies in
the owners, already onset before initiating the animal
treatment.
Conclusion: According to our experience the clini-cian must be
able to correctly identify the causative agents of skin problems in
rabbits. The increase of these patients casuistic as pets also
opens new pros-pects for diagnosis and new therapeutic challenges.
It is in our opinion, an opportunity to establish good veterinary
practice prevention and prophylaxis of these diseases, since the
good general health is con-ditioned by proper education and
information of the owner.
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Identificao de Parasitas de Peixes em Cortes Histolgicos
Ramos, P.1*, Carvalho, R.1, Rosa, F.2
1 Laboratrio de Patologia dos Animais Aquticos. Ins-tituto
Portugus do Mar e da Atmosfera, IPMA, I.P. Avenida de Braslia,
1449-006 Lisboa, Portugal
2 Instituto de Investigao Cientfica Tropical, IICT. Rua da
Junqueira n 14, 1300-343 Lisboa, Portugal
1 J no se encontra a trabalhar na Instituio* Autor
correspondente. Tel.: + 00351 213027000 Fax: + 00351 962467682
* [email protected]
Introduo: A identificao de parasitas de peixes em cortes
histolgicos poder apresentar alguma difi-culdade, sobretudo quando
as caractersticas morfol-
gicas usadas na sua identificao no so observveis. Material e
Mtodos: Apresentam-se trs casos de
infeco parasitria: peritoneu de safio, Conger con-ger, com
aspecto nodular; msculo de faneca, Trisop-terus luscus, com cor
negra e corao de perca sol, Le-pomis gibbosus, envolvido por
quistos de cor branca. Os tecidos foram fixados em formol salgado a
10% e processados histologicamente.
Resultados: A estrutura nodular de peritoneu cor-respondia a um
aglomerado de larvas enquistadas. A ausncia de progltides indicou
tratar-se de espcimes imaturos. Nuns cortes, o parnquima larvar
apresen-tava aspecto de tecido laxo, sem estruturas morfol-gicas e
noutros, observou-se o esclex com botrdeas e quatro tentculos
retrcteis armados com ganchos, bem como corpsculos calcreos
correspondendo a larvas plerocercides de Trypanorhyncha. No mesmo
tecido foram observadas larvas filiformes, com corpo
Um caso de localizao exclusivamente gstrica de Spirocerca lupi
numa raposa (Vulpes vulpes) em Portugal
Silva J.F.*, Madeira de Carvalho L., Moreira A.S.
CIISA - Faculdade de Medicina Veterinria (Universi-dade Tcnica
de Lisboa), Av. da Universidade Tcnica (Polo Universitrio Alto da
Ajuda), 1300-477 Lisboa
* [email protected]
Introduo: O Spirocerca lupi localiza-se caracte-risticamente na
parede esofgica de Candeos. Ferran-telli et al.(2010) e Diakou et
al.(2012), assinalaram a presena deste Nemtode na parede gstrica de
raposas.
Material e mtodos: O cadver de uma raposa adul-ta, sexo
masculino, pesando 5,85 kg foi necropsiado e colhido material, que
foi processado segundo a tcnica histolgica de rotina. A identificao
dos Nemtodes em cortes histolgicos foi feita de acordo com o artigo
de Chitwood e Lichtenfels (1972) e tendo em conta as-pectos
bioecolgicos e epidemiolgicos.
Resultados: Na parede do estmago observaram-se dois ndulos
coalescentes, com dimetros respectiva-mente de 1,2 cm e 1,8 cm.
Estes apresentavam super-fcie de corte branca, uma cavidade
contendo material necrtico e um Nemtode enrolado sobre si prprio,
de cor avermelhada, com cerca de 1 mm de espessura. O exame
microscpico dos ndulos revelou uma cpsu-la de tecido conjuntivo
fibroso envolvendo Nemtodes adultos. Estes apresentavam musculatura
celomiria e polimiria, cordes laterais proeminentes, tero
encer-rando ovos no operculados de casca fina, que conti-nham uma
larva. O intestino apresentava um epitlio simples cbico com
microvilosidades alongadas.
Concluso: As caractersticas histolgicas, bioe-colgicas e
epidemiolgicas do Nemtode permitem
classific-lo como Spirocerca lupi, pelo que este um caso de
localizao gstrica deste Nemtode em raposa em Portugal.
A case of location exclusively in the gastric wall of Spirocerca
lupi in a fox (Vulpes vulpes) in Portugal
Introduction: One of the Spirocerca lupi current lo-cations is
the oesophagic wall in Canidae. Ferrantelli et al. (201) and Diakou
et al. (2012) reported the gastric wall location of this Nematode
in foxes.
Material and methods: An adult male fox weight-ing 5.85 kg was
necropsied and samples were col-lected, which were processed
according to the routine histological technique. The identification
of Nematode specimens in histological sections was based in the
pa-per by Chitwood and Lichtenfels (1972) and consider-ing
bioecological and epidemiological aspects.
Results: In the gastric wall were observed two coa-lescent
nodules, which diameters were, respectively; 1.2 cm and 1.8 cm.
Their cut surface was white, with a cavity containing necrotic
material and a red Nema-tode coiled over itself, with 1 mm thick.
The micro-scopic exam of the nodules revealed a capsule of dense
connective tissue surrounding adult Nematoda. These worms possessed
muscle cells of the coelomyarian and polymyarian types, prominent
lateral chords, and an uterus containing thin-shelled,
non-operculated ova, with a larva inside. The intestinal wall
consisted of a simple cuboidal epithelium with long microvilli.
Conclusion: The histological, bioecological, and epidemiological
characteristics of this Nematode al-lowed us to classify it as
Spirocerca lupi. The present case relates to an exclusively gastric
location of this Nematode in a Portuguese fox.
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circular em corte transversal, esfago de aspecto sim-trico
radial e lmen triradial, correspondendo a larvas de nematodos.
No msculo de faneca identificaram-se ovos bioper-culados,
morulados ou castanhos embrionados, bem como pores de larvas ou
adultos com corpo ciln-drico localizados entre as fibras musculares
e dentro delas, resultando numa infeco parasitria por nema-todos
Trichosomoididae, Huffmanella spp.
No corao da perca sol, os quistos continham uma larva no
segmentada, com dois cecos, uma ventosa oral e outra ventral, do
trematode digentico, Postho-diplostomum minimum centrarchi.
Concluso: Na identificao dos parasitas foi im-portante observar
os caracteres morfolgicos, mas o conhecimento do peixe parasitado,
o seu habitat e os tecidos afectados, auxiliaram o diagnstico.
Identification of Fish Parasites in Stained Tissue Samples
Introduction: In stained tissue sections prepared for light
microscopy, taxonomic features may not be present, thus making
parasite identification difficult. Three cases of parasite
infection were described: Euro-pean conger, Conger conger
peritoneum with nodular appearance; pouting, Trisopterus luscus
with abnormal dark colour muscle and pumpkinseed sunfish, Lepomis
gibbosus heart, surrounded by white colour cysts.
Material and Methods: Tissues were fixed in 10% buffered neutral
formalin and processed using standard methods.
Results: The nodular peritoneum consists of multi-tude of
encysted and clustered larvae. The absence of complete segmentation
into proglottis identifies this specimen as immature. In sections,
the body larvae vary from loosely parenchyma to prominent scolex
features with bothridia and four tentacles armed with hooks. The
larvae were identified as Trypanorhynchean plerocercoid. In this
nodular peritoneum, filiform lar-vae were also present showing a
round body surface in cross-section and the histological features
of the oesophagus with its radial symmetry and triradiate lu-men
which allowed classifying them as nematodes.
The histopathological findings of the pouting mus-cle, revealed
bioperculated eggs, immature and embry-onated brown eggs. The eggs
and portions of larvae or adult nematodes Trichosomoididae of the
genus Huff-manella were observed in either intracellular or
extra-cellular locations.
The heart of pumpkinseed sunfish was found infected by
Posthodiplostomum minimum centrarchi larvae. The larvae were
differentiated by the absence of the body segmentation and by an
addition sucker, the ventral one.
Conclusion: The morphological characters are im-portant in
parasites identification but the knowledge on infected fish
species, their habitat and infected tissues also provide additional
information to the parasites identification.
Caracterizao de um surto da nova variante da Doena Hemorrgica
Viral (DHV) em coelhos bravos (Oryctolagus cuniculus algi-rus),
Barrancos, Sudeste de Portugal
Melo P.1*, Abrantes J.2,3, Ramada M. 1, Lopes A.M. 2,3,4, Alves
P.C. 2,4, Esteves P.J. 2,5, Correia J.J. 6
1 VETNATURA, Lisboa, Portugal *[email protected] CIBIO/UP,
Centro de Investigao em Biodiversidade e Recursos
Genticos/Universidade do Porto, InBio, Laboratrio Associado, Campus
Agrrio de Vairo, Vairo, Portugal
3 INSERM, U892, Universit de Nantes, Nantes, Fran-ce
4 Departamento de Zoologia e Antropologia, Faculdade de Cincias
da Universidade do Porto, Porto, Portugal
5 CITS, Centro de Investigao em Tecnologias da Sa-de, IPSN,
CESPU, Gandra, Portugal
6 CIISA, Faculdade de Medicina Veterinria - Universi-dade Tcnica
de Lisboa, Portugal
Introduo: A doena hemorrgica viral (DHV) do coelho tem como
agente etiolgico um calicivrus e causa elevada mortalidade em
coelhos domsticos e
bravos. Na Pennsula Ibrica, a DHV foi descrita em 1989 e todas
as estirpes virais isoladas pertenciam ao genogrupo 1. Em 2011
detectou-se em Espanha uma nova variante deste vrus, descrita
anteriormente em Frana, diferenciando-se por originar mortalidade
em juvenis (
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Principais dificuldades encontradas na aplicao das tcnicas de
deteo de apoptose em tecidos animais
Garcia C1*, Cruz R1,2, Mega A1,2, Oliveira AL3, Teixeira de
Lemos E1,2,5, Ferreira D4, Reis F5, Vala H1,2
1 Escola Superior Agrria de Viseu, Instituto Politcni-co de
Viseu, Portugal;
2 Centro de Estudos em Educao, Tecnologias e Sa-de, Instituto
Politcnico de Viseu, Portugal
3 Universidade de Lon, Lon, Espanha4 Laboratrio de Farmacologia
e Teraputica Experi-mental, IBILI, Faculdade de Medicina da
Universida-de de Coimbra, Coimbra, Portugal
5 CICV- Centro de Investigao em Cincias Veterin-rias,
Universidade Lusfona de Humanidades e Tec-nologia, Lisboa,
Portugal
* [email protected]
Introduo: A aplicao de metodologias capazes de identificar
clulas apoptticas constitui uma valiosa ferramenta em vrios estudos
biomdicos. Muito do conhecimento adquirido acerca dos mecanismos
regu-ladores da apoptose deriva de observaes em clulas isoladas. No
entanto, as propriedades demonstradas por estes ensaios nem sempre
se aplicam ao estudo de amostras tecidulares. Para deteo da
apoptose em tecidos, muitos investigadores recomendam o mtodo
TUNEL. Contudo, outros mtodos esto disponveis para avaliao da
apoptose, devendo a sua escolha ser criteriosamente avaliada, tendo
em conta as alteraes morfolgicas que se pretendem detetar. O
presente tra-balho pretende, como principal objetivo, partilhar as
dificuldades encontradas na implementao de dois diferentes mtodos
para deteo de clulas apoptticas em tecidos animais.
Material e mtodos: Foram utilizados tecidos de dife-rentes
animais (rato, porco e co), fixados em formalina a 10% e includos
em parafina, os quais foram submetidos a tcnicas imunohistoqumicas
com recurso ao mtodo TUNEL (Kit In situ cell death, Roche) e aos
anticorpos policlonais Bax e Bcl-2 (Santa Cruz Biotechnology).
Resultados: Aps um longo perodo de testes e de custos, em que se
obtiveram reaes inespecficas, bem como vrios artefactos - obstculos
que, para serem superados, requerem persistncia, todos os mtodos
provaram ser eficazes na deteo de apoptose, tendo sido as diferenas
de marcao observadas relaciona-das com o tipo de tecidos
testados.
Concluso: Os mtodos imunohistoqumicos cons-tituem uma abordagem
a considerar no que respeita deteo da apoptose em tecidos animais,
sendo que as dificuldades encontradas representam um desafio
su-pervel. Partilhar esta esperincia poder, certamente, evitar
testes e custos desnecessrios sua implementa-o noutras unidades
laboratoriais.
Agradecimentos: FCT e CI&DETS (PEst-OE/CED/UI4016)
A anlise filogentica demonstrou que as estirpes isoladas agrupam
com a nova variante com elevado suporte estatstico.
Concluses: Este estudo confirma a presena da nova variante em
Portugal e levanta questes quanto conservao e sustentabilidade das
populaes natu-rais de coelho bravo. Recomendam-se medidas
adicio-nais de gesto nas unidades de criao.
Characterization of an outbreak of the newly identified variant
of rabbit haemor-rhagic disease virus (RHDV) in wild rabbits
(Oryctolagus cuniculus algirus), Barrancos, Southeast of
Portugal
Introduction: Rabbit haemorrhagic disease virus (RHDV) is a
calicivirus that causes a highly fatal dis-ease in wild and
domestic adult European rabbits. In Iberian Peninsula, the first
description of RHDV dates from 1989 and all isolated strains
clustered within ge-nogroup 1. In 2011 a new variant of RHDV was
detect-ed in Spain, already described in France, that differs by
causing mortalities in young rabbits (
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Otimizao da imunodeteo do recetor HER2 em tumores mamrios de
cadela
Beselga, A. 1, Soares, M. 1, Carvalho, S. 1, Matos, J. 2,
Cabeadas, J. 2, Correia, J. 1, Peleteiro, M. 1, Ferreira, F. 1*
1 CIISA, Faculdade de Medicina Veterinria, Univer-sidade Tcnica
de Lisboa. Alto da Ajuda, 1300-477 Lisboa, Portugal
2 IPOFG-EPE, Servio de Anatomia Patolgica, 1099-023 Lisboa,
Portugal
* [email protected]
Introduo: O recetor para o fator de crescimento epidrmico de
tipo 2 (HER2) apresenta-se sobrex-presso em 15-30% dos tumores
mamrios na mulher, mostrando um elevado valor prognstico e
teraputi-co. Na cadela, o papel oncognico do HER2 perma-nece
duvidoso devido, em parte, falta de padroniza-o da tcnica de
imunohistoqumica.
Material e mtodos: Vinte e uma amostras de tumores mamrios
caninos (TMC) foram colhidas e fixadas de acordo com as indicaes da
American Society of Clinical Oncology (ASCO). Aps classi-ficao
histopatolgica, as amostras foram sujeitas a diferentes protocolos
imunohistoqumicos, tendo-se feito variar o mtodo de recuperao
antignica e o anticorpo primrio usado: TAB250, diluio 1:250
(Zymed); SP3, 1:100 (Zytomed); CB11, 1:60 (Invi-trogen); 4B5,
pronto a usar (Ventana). A interpreta-o dos resultados foi
realizada de acordo com os critrios da ASCO.
Resultados: Os melhores resultados foram obtidos
com o anticorpo CB11 quando associado ao mto-do de recuperao
antignica por panela de presso. Com este protocolo, 42,9% dos
tumores analisados mostraram sobrexpresso do recetor HER2. Todos os
anticorpos testados mostraram marcao membranar especfica no tecido
mamrio de cadela, exceo do clone TAB250.
Concluso: A variabilidade dos resultados obtidos demonstra a
necessidade de padronizao da tcni-ca de imunohistoqumica para deteo
do HER2 em Medicina Veterinria. Neste estudo, 42,9% dos TMC
analisados mostraram sobreexpresso do HER2, um percentagem superior
descrita na Mulher, reforan-do a possvel importncia deste
biomarcador em On-cologia Veterinria.
Optimization of HER2 receptor immuno-detection in canine mammary
tumours
Introduction: The epidermal growth factor type-2 receptor (HER2)
is overexpressed in 15-30% of breast tumors in women, presenting a
prognostic and high therapeutic value. In the bitch, the role of
the HER2 oncogene remains doubtful due to the lack of
immunohistochemistry technique standardization.
Material and methods: Twenty-one canine mam-mary tumors (CMT)
were collected and fixed in agreement to the American Society of
Clinical On-cology (ASCO) guidelines. After histopathological
classification, the samples were subjected to different
immunohistochemical protocols, varying the recov-ery antigen method
and the primary antibody used: TAB250, dilution 1:250 (Zymed); SP3,
1:100 (Zy-tomed); CB11, 1:60 (Invitrogen); 4B5, ready-to-use
Main difficulties encountered in implemen-tation of techniques
for apoptosis detection in animal tissues
Introduction: The implementation of methodolo-gies capable of
identifying apoptotic cells is a valu-able tool in many biomedical
studies. Much of the knowledge acquired about the mechanisms
regulat-ing apoptosis comes from observations in isolated cells;
however, the properties shown by these assays do not always apply
to the study of tissue samples. For detection of apoptosis in
tissues, many investi-gators recommend the TUNEL method,
nonetheless, other methodologies are available, and their choice
must be carefully evaluated, taking into account the morphologic
alterations which they intend to detect. The main goal of this work
is to share difficulties encountered in the implementation of two
different methods for the detection of apoptotic cells in animal
tissue.
Material and methods: Tissues from different ani-mals (rat, pig
and dog) tissues, fixed in 10% neutral-buffered formalin and
paraffin embedded were sub-mitted to immunohistochemical techniques
using the TUNEL method (In situ Cell Death Kit, Roche) and
polyclonal Bax and Bcl-2 antibodies (Santa Cruz
Bio-technology).
Results: After a long period of performed tests and expenses
made, in which unspecific reactions, as well as several artifacts
were obtained - obstacles that re-quired persistence to be
overcome, all methods have proven to be effective in the detection
of apoptosis, with differences probably related to the tissues
tested.
Conclusion: Immunohistochemical methods are a good approach to
consider in the detection of apoptosis in animal tissues. Sharing
the challenging difficulties found in our work, might obviate many
tests and expens-es in its implementation in other laboratory
facilities.
Acknowledgments: FCT and CI&DETS (PEst-OE/CED/UI4016)
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(Ventana). Results interpretation was performed ac-cording to
the ASCO criteria.
Results: The best results were obtained when the antibody CB11
was coupled with the pressure cooker as antigen retrieval method.
With this protocol, 42.9% of the analyzed tumors showed HER2
overexpression. All antibodies tested show specific immunostaing of
the cytoplasmic membrane, except the clone TAB250.
Conclusion: The variability of the results obtained demonstrates
the need of immunohistochemistry standardization for HER2
evaluation in Veterinary Medicine. In this study, 42.9% of TMC
analyzed showed HER2 overexpression, a higher percentage than the
one described in women, reinforcing the possible importance of this
biomarker in Veterinary Oncology.
Linfoma intestinal do tipo B com diferen-ciao em clulas de Mott
num gato.
Pedro Fasca1,2*; Maria Joo Costa3, Rita Pontes3, Dul-ce Carujo3,
Matilde Gonalves4, Marli Angua1, Ins Carvalho2
1 Faculdade de Medicina Veterinria da ULHT2 DNATech3 Clnica
veterinria de Telheiras4 Servio de Anatomia Patolgica Centro
Hospitalar de Setbal
* [email protected]
Introduo: Gato, macho, 11 anos de idade, que se apresentou
consulta para vacinao anual. No exa-me fsico detetou-se massa
abdominal, no dolorosa e firme.
Materiais e mtodos: Realizou-se ecografia abdo-minal que
confirmou massa intestinal.
Posteriormente foi realizada a sua remoo por en-terectomia.
Resultados: Macroscopicamente, ao nvel do jeju-no,
identificou-se neoformao ulcerada, no esteno-sante, com 9x5x4,5 cm;
em seco era branca amarela-da, brilhante, homognea, envolvendo toda
a espessura da parede intestinal.
Histologicamente observou-se proliferao difusa de clulas
linfoides desde a mucosa at serosa. As-sociado aos linfcitos
identificam-se inmeras clulas grandes e redondas, entre 20 a 30 m,
com ncleo em-purrado para a periferia e citoplasma preenchido por
glbulos eosinoflicos e hialinos, positivos com cido peridico de
Schiff, compatveis com clulas de Mott. Os linfcitos apresentaram
imunomarcao positiva para Pax5 (Leica NCL-L-PAX-5 1/80),
CD79alphacy (Dako M7051, 1/50) e imunomarcao negativa para CD3
(Dako A0452, 1/50); as clulas de Mott demons-traram positividade
para CD79alphacy, cadeias leves lambda (Ventana 760-2515) e
negatividade para Pax5, CD3 e cadeias leves kappa (Ventana
760-2514). De-monstrou-se ndice mittico baixo (0-2 mitoses por
campo com objetiva de 40x) e ndice de proliferao
celular superior a 30% com o anticorpo Ki-67 (Dako M7240
1/50).
Concluso: Com base nos achados morfolgicos, histoqumicos e
imunohistoqumicos, o diagnstico histolgico compatvel com linfoma
intestinal de tipo B, com diferenciao em clulas de Mott.
Feline intestinal B-cell lymphoma with Mott cell
differentiation.
Introduction: An 11year-old, male cat, was present for the
annual vaccination booster without any abnor-mal clinical signs. On
clinical examination, a firm, non-painful abdominal mass was
palpated.
Materials and methods: An abdominal ecography confirmed the
presence of an intestinal mass, which was posteriorly extirpated by
enterectomy.
Results: The jejunum presented a 9x5x4,5 cm, ho-mogenous, bright
whitish-yellow mass surrounding the intestinal lumen and invading
all intestinal layers.
Histologically, there was a diffuse non-capsulated proliferation
of round cells, disposed in dense sheets through all intestinal
layers. The neoplastic cells were of lymphoid type associated with
large (20-30um) round cells with cytoplasmatic eosinophilic,
periodic acidSchiff positive, globules compatible with Mott
cells.
The lymphoid cells presented positive immunostain-ing for
CD79alphacy (Dako M7051, 1/50), and Pax5 (Leica NCL-L-PAX-5 1/80),
and negative immunos-taining for CD3 (Dako A0452, 1/50); the Mott
cells presented positive immunostaining for CD79alphacy and lambda
light-chain (Ventana 760-2515 ready to use), and were negative for
Pax5, CD3 and kappa light-chain (Ventana 760-2514 ready to use).
The mitotic figures were rare (02 per 40x field of view), and the
Ki 67 proliferation index was higher than 30% (Dako M7240
1/50).
Conclusion: On the basis of histopathology, histo-chemistry and
immunohistochemistry, a B-cell lym-phoma with Mott cell
differentiation was diagnosed.
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Adenocarcinoma do endomtrio em gatas com menos de um ano de
idade: descrio de quatro casos
Pires M.A.1*, Saraiva A.L. 1,2, Santos T.3, Vilhena H.4, Sousa
A.5, Santos C.1, Payan-Carreira R.1
1 CECAV, University of Trs-os-Montes and Alto Dou-ro, P.O. Box
1013, 5001-801 Vila Real, Portugal.
2 EUVG Escola Universitria Vasco da Gama, 3040-714 Castelo
Viegas, Coimbra, Portugal.
3 Veterinary Teaching Hospital (HVUTAD), University of
Trs-os-Montes and Alto Douro, P.O. Box 1013, 5001-801 Vila Real,
Portugal.
4 Hospital Veterinrio do Baixo Vouga, 3750-742 gueda,
Portugal
5 Policlnica Veterinria de Aveiro - Vetsanus, 3800-118 Aveiro,
Portugal.
* [email protected]
Introduo: As neoplasias uterinas de origem epi-telial so raras
em todas as espcies animais, exceto em vacas e coelhas. Nas gatas,
o adenocarcinoma do endomtrio (AEG) , geralmente descrito em fmeas
com idade superior a 8 anos, ainda que, esporadica-mente, possa ser
observado em idades compreendidas entre os 2 e os 4 anos.
Material e mtodos: foram enviados ao Laboratrio de Histologia e
Anatomia Patolgica da UTAD 4 peas de ovariohisterectomia (OVH) de
gata, provenientes de diferentes Hospitais Veterinrios. Os rgos,
fixa-dos em formol tamponado a 10% foram processados por rotina
para parafina e corados com hematoxilina e eosina.
Resultados: A sintomatologia observada foi vari-vel, desde a
ausncia at presena de corrimento he-morrgico acastanhado,,
facilmente confundidos com pimetra ou aborto.
Macroscopicamente os teros apresentavam-se dis-tendidos, firmes,
com parede espessada, dilataes e irregularidade dos cornos uterinos
em diferentes graus. Microscopicamente observou-se proliferao
papilar do endomtrio, com invaso do miomtrio em dois dos casos. O
diagnstico foi de AEG de tipo papilar seroso.
Concluso: Embora as neoplasias de origem epi-telial do tero
sejam raras nos animais domsticos, o nosso grupo nos ltimos anos
tem registado mais AEG
do que o documentado. Embora esta neoplasia seja mais frequente
em animais velhos, nos ltimos 2 anos tivemos 4 casos de AEG em
gatas de idade inferior a um ano no momento da cirurgia.
Esta situao clnica pode ser confundida com abor-to ou pimetra,
pelo que se recomenda a anlise siste-mtica de todas as peas de OVH,
seja qual for a idade ou diagnstico clnico.
Feline Endometrial Adenocarcinoma in fe-males under one
year-old: A description of four cases
Introduction: Uterine neoplasms of epithelial origin are rare in
all domestic animals, except for cows and rabbits. In female cats,
endometrial adenocarcinoma (FEA) is usually described in females
over 8 years-old. Yet, sporadically it may be found in younger
females of age of 2-4 years.
Material and methods: 4 ovariohysterectomy surgi-cal specimens
from queens aged under one year old, at the time of surgery, were
submitted from different practices to the Laboratory of Histology
and Anatomi-cal Pathology, at UTAD. The organs were fixed in 10%
buffered formalin, processed to paraffin embed tissue, and stained
with haematoxylin and eosin.
Results: Different clinical symptoms were observed in these
animals, ranged from absent to a haemorrhagic or brownish
discharge, easily confused with pyometra or abortion.
Macroscopic outcomes revealed a distended and firm uterus, with
wall thickness, dilatations and irregularity along both uterine
horns, at different grades. Micro-scopic findings revealed a
papillary proliferation of the endometrium with myometrium invasion
in two cases. The final diagnosis was FEA of the papillary
type.
Conclusion: Although uterine neoplasms of epithe-lial origin are
rare in domestic animals, in the last years our group has recorded
more FEA than documented in the literature. Despite this neoplasia
to be related with older animals, in less than two years, we
untypically diagnosed 4 FEA in queens aged under one year-old at
time of surgery that could be clinically mistaken for pyometra or
abortion. For this reason, we strongly rec-ommend histopathological
analysis of all ovariohys-terectomy surgical specimens, despite
animals age or clinical diagnose.
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Abate de Lagomorfos nos Matadouros Portugueses: Estudo
Descritivo (2011-2012)
Jesus J.1, Santos S.1*, Lamela M.2
1 Centro de Investigao em Cincias Veterinrias
(CICV) - FMV- ULHT, Lisboa, Portugal2 Chefe de Diviso na Direco
de Servios de Segu-rana Alimentar da Direco Geral de Alimentao e
Veterinria (DGAV)
* [email protected]
Introduo: Este trabalho d a conhecer os volumes de abate actuais
de lagomorfos e as principais causas de reprovao sanitria nos
matadouros portugueses.
Material e mtodos: Analisou-se a totalidade de abates de
lagomorfos entre 1 de Janeiro de 2011 e 31 de Dezembro de 2012,
registados no Sistema de Infor-mao do Plano de Aprovao e Controlo
dos Estabe-lecimentos (SIPACE), plataforma informtica da Dire-o
Geral de Alimentao e Veterinria, utilizada por todos os inspetores
sanitrios desde Outubro de 2010.
Resultados: Foram apresentados para abate regular 10 901 526
lagomorfos. Desses, 321 (0,03) foram rejeitados ante-mortem e 93
665 (8,6) post-mortem. De entre os principais tipos de processos
patolgicos observaram-se leses fibrino-purulentas, abcessos e
caquexia. Registaram-se 20 497 mortes no transporte.
Concluso: No perodo em anlise, o volume de abate manteve-se
estvel e no apresentou sazonali-dade, sugerindo assim um consumo
regular desta car-ne ao longo do ano. As leses fibrino-purulentas e
os abcessos foram as principais causas de reprovao. A identificao
das causas de reprovao fundamental
para possibilitar a implementao de medidas preven-tivas.
Slaughtering of Lagomorphs in the Portu-guese Slaughterhouses:
Descriptive Study (2011-2012)
Introduction: The present study was conducted to evaluate
lagomorphss slaughtering data such as the current number of
slaughtered animals and the major causes to condemnation in
Portugal.
Material and methods: This study includes the total number of
lagomorphs slaughtered between 1st January 2011 and 31st December
2012. The data were obtained from Informatiom System of
Establishments Approval and Control Plan (SIPACE) software,
be-longing to Directorate General of Food and Veterinary (DGAV),
where all veterinary inspectors insert the records since October
2010.
Results: Out of 10 901 526 slaughtered lagomorphs, 321 (0,03)
were rejected ante-mortem and 93 665 (8,6) were rejected
post-mortem. The majority of injuries were fibrinopurulent lesions,
abscesses and cachexia. Deaths in the transport accounted 20 497
animals.
Conclusion: In the past two years the number of slaughtered
lagomorphs remained stable and no sea-sonality in the number of
slaughtered animals was noted, meaning that this type of meat is
consumed regularly over the year. Fibrinopurulent lesions and
abscesses were the most frequent causes of condemna-tion.
Preventive actions can only be taken after identi-fication of the
prevalent injuries, proving that studies like this are useful.
Caracterizao da populao linfocitria em leses granulomatosas de
javali (Sus scrofa) com diagnstico presuntivo de tu-berculose
Matos A.C1., Andrade S.2, Pires M.A.2*, Coelho A.C.2, Pinto
M.L.2
1 Instituto Politcnico de Castelo Branco, Castelo Branco,
Portugal
2 CECAV - Centro de Cincia Animal e Veterinria; ECAV- Escola de
Cincias Agrrias e Veterinrias, Vila Real, Portugal
* [email protected]
Introduo: A tuberculose doena infeciosa que ameaa a vida de
milhes de pessoas, quer em pases
em desenvolvimento, quer em pases desenvolvidos, sendo tambm
responsvel por elevadas perdas econ-micas em animais de espcies
pecurias. A patognese da tuberculose bovina, causada por
Mycobacterium bo-vis e da tuberculose humana, cujo agente principal
o microrganismo da espcie Mycobacterium tuberculo-sis, so as que se
encontram melhor caracterizadas. No entanto, a tuberculose tambm
afeta outras espcies de animais, sendo estes muitas vezes os
responsveis pelo contgio s espcies pecurias e ao Homem. Em
par-ticular, o estudo da tuberculose nas espcies selvagens pode
elucidar a forma como esta doena se mantm e se transmite.
Material e mtodos: Este trabalho foi realizado com o objetivo de
avaliar a resposta inflamatria crnica em animais selvagens com
diagnstico presuntivo de tuberculose, em que se procurou investigar
a expres-so dos anticorpos anti-CD3 e anti-CD79 em leses
Poster presentations
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Estudo Preliminar sobre a Descalcificao de Tecido sseo em
Micro-ondas
Angua M.*, Fasca P.
Centro de Investigao em Cincias Veterinrias- Fa-culdade de
Medicina Veterinria - Universidade Lus-fona de Humanidades e
Tecnologias
* [email protected]
Introduo: A anlise histopatolgica de tecido s-seo exige uma
etapa de descalcificao, que possibilite a microtomia. O mtodo mais
comum consiste na imerso das amostras em solues cidas, mas para alm
de pro-vocarem danos tecidulares, o processo muito prolon-gado, o
que atrasa significativamente o diagnstico. A utilizao de
micro-ondas acelera a descalcificao, mas no deve comprometer a
imagem microscpica.
O objetivo deste trabalho consistiu na diminuio da durao da
descalcificao, mantendo a qualidade da imagem microscpica.
Material e mtodos: Foram testadas amostras de osso compacto e
esponjoso, provenientes de caprinos, com dimenses que pretenderam
simular casos de bi-psias e casos de peas cirrgicas ou necrpsias.
Re-alizou-se a descalcificao pelo mtodo convencional
e pelo mtodo em micro-ondas, atravs da adaptao de um protocolo
conhecido. Utilizou-se cido ntrico a 5% e 10%.
Resultados: Nos fragmentos de maiores dimenses, o protocolo foi
interrompido aps 4 horas com cido ntrico a 10% em micro-ondas, por
no se conseguir uma descalcificao completa e estar a ocorrer
destrui-o do osso compacto. Nos fragmentos de osso espon-joso,
verificou-se uma reduo de cerca de 25 horas relativamente ao mtodo
convencional. Nas bipsias, houve uma reduo de aproximadamente 10
horas, uti-lizando cido ntrico a 5%. Com cido ntrico a 10% houve
destruio tecidular. Nos casos em que se obteve uma descalcificao
completa, a imagem microscpica apresenta fraca qualidade.
Concluso: A utilizao de micro-ondas na descal-cificao com cido
ntrico a 5% ou 10%, aplicando o protocolo deste estudo em amostras
de caprinos, reduz a durao do processo, mas compromete
significativa-mente a imagem microscpica, o que se torna invivel
para diagnstico.
Preliminary Study about Microwave De-calcification of Bone
Tissue
Introduction: Histological analysis of bone tissue
granulomatosas dos gnglios mesentricos de javalis (n=30), pela
tcnica de imuno-histoqumica.
Resultados: Pela anlise imuno-histoqumica ob-servou-se marcao
citoplasmtica das clulas lin-focitrias em 11 gnglios mesentricos
(36,6%) para o anticorpo anti-CD79, e em 15 gnglios mesent-ricos
(50%) para o anticorpo anti-CD3. A quantifica-o dessas mesmas
clulas permitiu agrupar as leses consoante a percentagem de clulas
linfocitrias que exibiam.
Concluso: As leses em estudo apresentam percen-tagens
relativamente semelhantes de linfcitos T e B, sugerindo assim uma
resposta celular e humoral com igual participao na resposta
imunitria, desencadea-da pelo organismo face entrada do agente
patognico.
Lymphocyte population in the granuloma-tous lesions of
wild-boars (Sus scrofa) sus-pected of tuberculosis.
Introduction: Tuberculosis is an infectious disease that
threatens the lives of millions of people both in developing and
developed countries and is responsible for important economical
losses in farm animals. The pathogenesis of bovine tuberculosis due
to Mycobac-terium bovis and human tuberculosis mainly caused by the
microorganism of the species Mycobacterium
tuberculosis are more extensively characterized. How-ever,
tuberculosis also affects other animal species and these are often
responsible for the contamination of cattle and humans. In
particular, an overview of the wildlife hosts will provide insight
into how these reser-voirs maintain and spread the disease.
Material and methods: This study was conducted to evaluate the
chronic inflammatory response in wild animals with a presumptive
diagnosis of tuberculosis. In order to better understand the
pathogenesis of the disease and further characterize its lesions,
it was our aim to investigate the expression of antibodies anti-CD3
and anti-CD79 in the granulomatous lesions of wild boars mesenteric
lymph nodes (n=30) through the immunohistochemistry technique.
Results: Immunohistochemical analysis allowed us to observe the
lymphocyte cells population in 11 me-senteric nodes (36.6%) for the
anti-CD79 antibody, and in 15 mesenteric nodes (50%) for the
anti-CD3 an-tibody. The quantification of these same cells allowed
grouping of lesions depending on the percentage of lymphocyte cells
that they exhibit.
Conclusion: The lesions of this study showed rela-tively similar
percentages of B and T lymphocytes, thus suggesting a cellular and
humoral response and a similar immune response triggered by the
organism against the entry of the pathogen.
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Necrpsia em equinos. Descrio de um caso de doena granulomatosa
disseminada
Miranda I.*, Tilley P., Pomba C., Pissarra H., Correia J.J.,
Peleteiro M.C.
Centro de Investigao Interdisciplinar em Sanidade Animal,
Faculdade de Medicina Veterinria, Univer-sidade Tcnica de Lisboa.
Alto da Ajuda, 1300-477 Lisboa, Portugal
* [email protected]
Introduo: Tendo em conta a importncia de ava-liar a prevalncia
das situaes ocorridas e a capacida-de de emisso de diagnsticos
definitivos, procedeu-se identificao das causas de morte dos casos
de ne-crpsia de cavalos registados no Laboratrio de Anato-mia
Patolgica da Faculdade de Medicina Veterinria, da Universidade
Tcnica de Lisboa. De entre os casos identificados foi dado
particular relevo a um de doena granulomatosa disseminada (DGD),
atendendo rari-dade do processo, complexidade da evoluo clnica e
aos diagnsticos diferenciais envolvidos.
Material e mtodos: Foram analisados os registos compreendidos
entre Janeiro de 2009 e Janeiro de 2013, num total de 38 necrpsias.
No caso da DGD, o diagnstico baseou-se na anlise histopatolgica de
r-gos fixados em formol e submetidos a processamento histolgico de
rotina. As coloraes efetuadas foram H&E e Ziehl Neelsen. Foi
ainda realizada anlise bac-teriolgica.
Resultados: Dos 39 casos de necrpsia analisados, apenas um foi
inconclusivo. Os restantes foram divi-didos em seis grupos: Doena
Infeciosa Sistmica (5), Doena Cardiorrespiratria (5), Doena Renal
(1), Do-ena Gastrointestinal (21), Doena Neurolgica (3), Doena
Ortopdica/Trauma (3). Os exames comple-mentares realizados foram
anlise histopatolgica (24) e anlise bacteriolgica (10).
O diagnstico de doena granulomatosa dissemina-da compatvel com
sarcoidose equina baseou-se nas le-ses identificadas na necrpsia e
na anlise histopato-lgica de leses granulomatosas presentes em
diversos rgos, ricas em clulas gigantes de Langhans e negati-vas
para a presena de bacilos lcool-cido resistentes.
Concluso: Como concluso, pode referir-se que re-alizao de
necrpsia permite, na grande maioria dos casos, identificar a causa
da morte em equinos, pre-ferencialmente com recurso a outros exames
comple-mentares de diagnstico.
Necropsy in horses. A case of disseminated granulomatous
disease
Introduction: With the objective of estimating the prevalence of
the occurrences and the capability to is-sue definitive diagnosis,
an evaluation of the various causes of death in horses was
performed based on the necropsy cases registered in the Laboratory
of Pathol-ogy, Faculty of Veterinary Medicine, Technical
Univer-sity of Lisbon. Among the cases identified, particular
emphasis was given to a case of disseminated granu-lomatous disease
(DGD), due to the rarity of the pa-thology, complexity of the
clinical evolution and the differential diagnosis involved.
Material and methods: The reports from January 2009 to January
2013 were analyzed, and a total of 39 necropsies recovered. In the
case of DGD the conclu-sion was based on the histopathology of the
organs fixed in 10% formalin and subject to regular histologi-cal
processing. H&E and Ziehl Neelsen stains were performed.
Bacteriology was also performed.
Results: Out of 39 cases of necropsy analyzed, only one was
inconclusive. The remainders were divided into six groups: Systemic
Infectious Disease (5), Car-diopulmonary Disease (5), Kidney
Disease (1), Gas-trointestinal Disease (21), Neurological Disease
(3) and Trauma/Orthopedic Disease (3). Complementary
requires a decalcification process. The most common method
consists on samples immersion in acidic so-lutions. However, it
causes tissue damage and is time consuming, delaying the diagnosis.
The use of mi-crowaves accelerates the decalcification process, but
shouldnt compromise the histological image.
The aim of this work was to reduce the decalcification time,
maintaining the quality of the histological image.
Material and methods: Goat compact and spongy bone, with
dimensions that intended to simulate bi-opsies and surgical or
necropsies specimens, were used. Decalcification was performed by
conventional method and microwave method with an adaptation on a
known protocol, using 5% and 10% nitric acid.
Results: For larger fragments, the protocol was dis-
continued after 4 hours of microwave cycles** with 10% nitric
acid, because the decalcification was in-complete, in spite of
evident compact bone destruc-tion. In the fragments of spongy bone,
there was a reduction of approximately 25 hours, comparing with
conventional method. In biopsies, there was a reduc-tion of
approximately 10 hours using 5% nitric acid. With 10% nitric acid,
there was tissue destruction. The cases with complete
decalcification had a poor quality of the histological image.
Conclusion: The use of microwave decalcification with 5% or 10%
nitric acid, applying the protocol of this study on goats samples,
reduces the process time but compromises the histological image,
which is im-practical for diagnosis purpose.
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Processos patolgicos prevalentes em carnvoros silvticos mortos
em acidentes rodovirios
Matos A.C1., Figueira L1., Martins M.1, Matos M.2, l-vares S.1,
Mendes A.1, Pires M.A.3, Coelho A.C.3, Pinto M.L.3*
1 Instituto Politcnico de Castelo Branco, Castelo Branco,
Portugal
2 Centro de Gentica e Biotecnologia, Instituto de Bio-tecnologia
e Bioengenharia, Universidade de Trs-os-Montes e Alto Douro, Vila
Real, Portugal
3 CECAV - Centro de Cincia Animal e Veterinria; ECAV- Escola de
Cincias Agrrias e Veterinrias, Universidade de Trs-os-Montes e Alto
Douro, Vila Real, Portugal
* [email protected]
Introduo: As doenas que afetam os animais selvagens tm sido alvo
de um interesse crescente,
uma vez que se demonstrou que, para alm das impli-caes
relacionadas com a conservao das espcies, podem ter um impacto
significativo na sade animal e humana. Devido ao seu comportamento
furtivo, os carnvoros selvagens constituem uma populao difcil de
monitorizar e a investigao de doena nestas es-pcies resulta
geralmente do exame de animais que so encontrados mortos.
Material e mtodos: Este estudo foi realizado em carnvoros
selvagens vtimas de acidentes de viao, em Portugal, entre 2009 e
2012, e inclui 3 famlias e 6 espcies diferentes (n=72). Os animais
foram submeti-dos a exame post-mortem e foram efetuadas colheitas,
sendo os tecidos fixados em formol tamponado a 10%, processados
para incluso em parafina e corados com hematoxilina-eosina.
Resultados: As leses pulmonares foram as mais prevalentes,
(15/72; 20,8%), especialmente em ra-posas (Vulpes vulpes, 8/49;
16,3%) e saca-rabos (Herpestes ichneumon, 7/15; 46,6%). As
pneumonias parasitrias, sugestivas de infestao por Crenosoma vulpis
ou Angiostrongylus vasorum foram observadas
Fibromatosis in Iberian hare Lepus granat-ensis. Preliminary
data.
Arnal MC, Martnez-Durn D, Revilla M, Fernndez de Luco D *
Departamento de Patologa Animal. Facultad de Vet-erinaria.
Universidad de Zaragoza. C/ Miguel Servet 177. 50013 Zaragoza.
Spain.
* [email protected]
Introduction: Hare fibromatosis is a rare and trans-missible
viral disease, caused by a Leporipoxvirus, antigenically more
related to the Shope fibroma virus than myxoma virus. The disease
is characterized by single or multiple skin tumours on ears, head
and legs. The affected animals appear in late summer and au-tumn
with high morbidity and low mortality. Fibroma-tosis has been
described in wild and farmed brown hare Lepus europaeus in Europe
(France, Germany, Italy, U.K.). African hare fibromatosis is a very
similar disease caused by an unclassified Poxvirus affecting Cape
hare Lepus capensis in Kenya. Recently, another
similar disease has been reported in mountain hare Le-pus
timidus in Finland, where gross and microscopic lesions resembled
to be associated to a Poxvirus infec-tion.
Material and methods: We describe here two natu-ral cases in
wild Iberian hares Lepus granatensis in Aragon region (Spain). Both
hares were adults female and male hunted in the autumn period in
1998 and 2006, respectively.
Results: Noticeable swelling eyelids were observed in both
animals and the male also showed small nodu-lar lesions in nose and
lips, swelling prepuce and an irregular swelling of scrotum skin.
The dermis histopa-thology of skin gross findings consisted in
fibroblast cells proliferation and oedema, whereas the epidermis
showed swelling and vacuolated cells with intracyto-plasmic
eosinophilic inclusion bodies. Infiltration of plasma cells and
lymphocytes were observed in the dermis. Neutrophils were present
in the dermis and epidermis, probably by secondary bacterial
infections.
Conclusion: The observed lesions could be associat-ed to hare
fibromatosis in Iberian hare Lepus granat-ensis, in Spain.
exams were histopathological examination (24) and
bacteriological analysis (10).
The diagnosis of disseminated granulomatous dis-ease consistent
with equine sarcoidosis was based on the gross morphology at
necropsy and histopathology of granulomatous lesions with Langhans
giant cells
present in different organs that were negative for the presence
of acid-fast bacilli.
Conclusion: It can be concluded that, in most cases, necropsy
allows for the identification of the cause of death in horses,
preferentially using other complemen-tary exams to achieve a
correct and complete diagnosis.
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Doena vrica hemorrgica (DVH) uma nova variante?
Vala H1,2*, Coelho C1,2, Nbrega C1,2, Mesquita JR1,2, Oliveira
J1,2
1 Escola Superior Agrria de Viseu, Instituto Politcni-co de
Viseu, Portugal;2 Centro de Estudos em Educao, Tecnologias e Sa-de,
Instituto Politcnico de Viseu, Portugal
* [email protected]
Introduo: A doena vrica hemorrgica (DVH), causada por um RNA
vrus (gnero Lagovrus, famlia Caliciviridae), uma doena fatal que
afeta coelhos do-msticos e selvagens. Estudos anteriores a 1984,
demons-traram a existncia de anticorpos especficos para DVH em
coelhos saudveis de vrias regies europeias e ainda a possibilidade
de estes animais apresentarem alguma re-sistncia contra a DVH
conferida por uma imunidade cru-zada com calicivrus benignos,
fazendo com que a doena exibisse um quadro clnico menos severo. A
doena foi controlada em cuniculturas graas vacinao, mas ain-da uma
ameaa nas populaes selvagens no vacinadas.
Uma nova variante desta doena, descrita em 2010 na Pennsula
Ibrica, afeta principalmente animais jo-vens, que exibem
hemorragias no corao, traqueia, timo, pulmes, fgado, rins,
intestino, assim como
CID, ictercia, esplenomeglia e enterite catarral.Objetivos:
Descrever um surto de elevada morta-
lidade numa cunicultura, principalmente em animais jovens, com
leses tpicas de DVH.
Material e mtodos: Foram colhidas amostras de co-elhos vacinados
e no vacinados, alvo de morte sbita. As amostras foram fixadas em
formalina a 10% (48 ho-ras), embebidas em parafina e processadas
seguindo os procedimentos de rotina para avaliao histopatolgica
Resultados: Principais leses observadas: opist-tonus; congesto e
edema pulmonar; ictercia severa; esplenomeglia; lceras gstricas
punctiformes; con-gesto renal bilateral, petquias (timo).
Concluso: Embora esta infeo seja mais comum em animais adultos,
as leses observadas so consis-tentes com DVH. A presena
concomitante de leses associadas nova variante de DVH (CID,
ictercia, esplenomeglia, enterite catarral) leva-nos a suspeitar da
sua presena em Portugal, ressalvando o facto da necessidade de
estudos mais aprofundados.
Agradecimentos: FCT e CI&DETS (PEst-OE/CED/UI4016/2011)
Rabbit haemorrhagic disease (RHD) a new variant?
Introduction: Rabbit haemorrhagic disease (RHD) is an acute
fatal disease of domestic and wild Euro-
em 6 raposas. A adiaspiromicose, provocada por Em-monsia
crescens, foi a leso pulmonar mais comum em saca-rabos (4/15,
26,6%). A nefrite intersticial crnica e a linfadenite macrofgica
afetaram, respetivamente, 10/72 (13,8%) e 6/72 (8,3%) animais das
famlias Ca-nidae e Mustelidae.
Concluso: De acordo com este estudo, as pneu-monias parasitrias
e micticas encontram-se entre as leses mais prevalentes em
carnvoros selvagens e podem ter um impacto significativo na sade
destas es-pcies, quer devido magnitude das leses observadas, quer
devido ao aumento da susceptibilidade a outros agentes
infeciosos.
Disease processes identified in road killed wild carnivores
Introduction: Wildlife diseases have been considered
increasingly important due to their potential effect upon human
health, veterinary medicine, wildlife, and con-servation biology.
Since wild carnivores are elusive ani-mals, the occurrence of
disease in these species has been typically investigated by
identification of pathological processes in carcasses that are
found incidentally.
Material and methods: This study was conducted in wild
carnivores found death on the roads in Portu-
gal, from 2009 to 2012 (n=72), representing 3 families and 6
different species. Post mortem examination was performed and
multiple tissues were fixed in 10% neu-tral buffered formalin,
processed for routine paraffin embedding and stained with
hematoxylin and eosin for histopathological examination.
Results: Lung lesions were the most prevalent find-ing (15/72,
20.8%), particularly in fox (Vulpes vulpes, 8/49, 16.3%) and
Egyptian mongooses (Herpestes ichneumon, 7/15, 46.6%). Six foxes
showed lesions of parasitic pneumonia suggestive of Crenosoma
vul-pis or Angiostrongylus vasorum infestation. In Egyp-tian
mongooses adiaspiromycosis due to Emmonsia crescens were the most
common lung lesions (4/15, 26.6%). Chronic interstitial nephritis
was registered in 10/72 (13.8%) animals, while in 6/72 (8.3%)
macro-phage lymphadenitis was observed, both conditions af-fecting
members of the Canidae and Mustelidae.
Conclusion: According to our study, parasitic and mycotic lung
pneumonias are amongst the most preva-lent lesions in wild
carnivores. Although parasites and fungus may often be regarded as
a burden that has little or no impact on the animals, our findings
suggest that they can severely affect animal health by the
magni-tude of their lesions and by making turning the host more
susceptible to other infectious agents.
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Timo de Coelho um potencial marcador para tcnicas de deteo de
apoptose
Vala H1,2*, Santos C1,2, Cruz R1,2, Nbrega C1,2, Ortiz AL3,
Mesquita JR1,2, Esteves F1,2, Mega A1,2,
1 Escola Superior Agrria de Viseu, Instituto Politc-nico de
Viseu, Portugal;
2 Centro de Estudos em Educao, Tecnologias e Sade, Instituto
Politcnico de Viseu, Portugal
3 Len University, Len, Spain
* [email protected]
Introduo: As tcnicas de determinao de apop-tose tm sido
preconizadas para fins de diagnstico, designadamente para
determinao das caratersticas biolgicas das neoplasias e,
indiretamente, determi-nao do prognstico.
A implementao de uma nova tcnica num labo-ratrio pressupe o uso
de um controlo positivo efi-caz para aferio da mesma. Muitas das
publicaes da especialidade sobre o tema no indicam, de modo claro,
os controlos positivos usados e no divulgam os resultados dos
mesmos.
O objetivo deste trabalho consistiu na implemen-tao de trs
mtodos de deteo de apoptose, utilizan-do trs amostras de tecidos de
co: neoplasia mamria - carcinoma in situ, timo e plasmocitoma; e
uma amos-tra de tecidos de coelho timo.
Material e mtodos: Os mtodos de imunohis-toqumica incluram a
deteo de protenas pro- e anti-apoptticas, respetivamente, Bax e
Bcl-2 (1:100; Santa Cruz, Biotechnology) e o mtodo de TUNEL, com o
kit de deteo de apoptose in situ (1:2,5; Roche).
Resultados: O plasmacitoma revelou reao posi-tiva moderada com
todos os mtodos testados. O car-
cinoma in situ revelou reao positiva moderada com os anticorpos
pro-apoptticos e mais intensa com o anticorpo anti-apopttico. O
timo de co revelou reao positiva moderada nos timcitos do crtex com
os anticorpos pr-apoptticos e dbil nos da medular. Com o anticorpo
anti-apopttico revelou reao dbil na medular e negativa no crtex.
Idnticos resultados foram obtidos no timo de coelho, tendo sido,
contudo, mais forte a intensidade das respetivas reaes.
Concluso: O timo de coelho revelou a reao mais especfica dos
tecidos estudados, sendo assim reco-mendado como um bom controlo
positivo para estudos de apoptose.
Agradecimentos: FCT e CI&DETS (PEst-OE/CED/UI4016/2011)
Rabbit thymic tissue a potential marker for apoptosis
techniques
Introduction: Apoptosis methods have been pro-posed for
diagnostic purposes, in particular for deter-mining the biological
characteristics of tumours and, indirectly, their prognosis.
Implementation of a new technique requires the use of an
effective positive for correct assessment.
Most specialty publications on the subject, do not indicate the
positive controls used and do not disclose the obtained results
therein.
Objective: The aim of this work was to implement three different
methods for the detection of apoptosis, using three canine tissue
samples, consisting of breast cancer - carcinoma in situ, thymus
and plasmacytoma; and a sample of rabbit tissues - thymus.
Material and methods: Immunohistochemistry was performed, using
pro- and antiapoptotic proteins, respectively, Bax and Bcl2 (1:
100; Santa Cruz, Bio-
pean rabbits caused by a RNA virus (genus Lagovirus, family
Caliciviridae). Prior to 1984, healthy rabbits in European regions
had RHDV-specific antibodies and their infection with benign rabbit
caliciviruses con-ferred them a low degree of cross-protective
immunity against RHD, determining less severe clinical courses.
Vaccination allowed to control the disease in the rab-bit industry,
but RHD still threatens non-vaccinated wild populations. A new
variant of this disease is be-ing described since 2010 in Iberian
Peninsula. It af-fects mainly young rabbits that exhibit
haemorrhages in heart, trachea, thymus, lungs, liver, kidneys and
gut, as well as DIC, jaundice, splenomegaly and catarrhal
enteritis.
Objectives: To describe a case of high mortality rate on a
rabbitry, mostly in young rabbits, with typical le-sions of
RHD.
Material and methods: Samples from domestic
vaccinated and non vaccinated rabbits which died sud-denly were
taken and fixed in 10% neutral-buffered formalin for 48 hours and
embedded in paraffin. 3m sections were stained for routine
histopathological di-agnosis (H&E) and examined by light
microscopy.
Results: Main macroscopic lesions observed: opisthotonos, oedema
and pulmonary congestion, se-vere jaundice, splenomegaly, gastric
punctiform ul-cers, bilateral renal congestion and thymic
petechiae,
Conclusion: Macroscopic lesions in infected youngs, consistent
with RHD infection usually ob-served only in adult rabbits, and the
presence of le-sions mostly associated to the new variant of RHD
(DIC, jaundice, splenomegaly, catarrhal enteritis), led us to
consider the presence of the new variant of RHD in Portugal,
although further studies are needed.
Acknowledgments: FCT and CI&DETS
(PEst-OE/CED/UI4016/2011)
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SUPLEMENTO RPCV (2013) 108 (585-586) 69-91
87
Um caso de hemangiosarcoma cardaco em co
Pires M.A.1,2*, Pinheiro D. 1, Silva N.3, Montenegro L.3
1 CECAV, University of Trs-os-Montes and Alto Dou-ro, P.O. Box
1013, 5001-801 Vila Real, Portugal.
2 ECAV, University of Trs-os-Montes and Alto Douro, P.O. Box
1013, 5001-801 Vila Real, Portugal.
3 Veterinary Montenegro Hospital (HVM), 4200 - 447 Porto,
Portugal.
* [email protected]
Introduo: Segundo a literatura, o hemangiossar-coma cardaco uma
situao comum nos ces Pastor Alemo, comumente associado a leso
esplnica.
Os autores descrevem um caso de hemangiossarco-ma cardaco sem
envolvimento esplnico, com par-metros clnicos e imagens
macroscpicas, associado a metstases pulmonares.
Material e mtodos: Foram remetidos ao Laborat-rio de Histologia
e Anatomia Patolgica da UTAD, o corao e pulmo de um Pastor Alemo,
macho, de 11 anos de idade. Os rgos fixados em formol tampona-do a
10%, foram processados por rotina e corados com hematoxilina e
eosina.
Resultados: O historial do animal indicava ausncia de vacinao e
desparasitao, fraqueza extrema, disp-neia e choque. Ao RX a
silhueta cardaca apresentava-se arredondada e com imagens de efuso
torcica e pericrdica, confirmada por ecografia.
Foi solicitada a eutansia, tendo a necrpsia revelado hemotrax e
hemopericrdio, leso papilar exofitica na aurcula direita, vilosa,
de 3 cm de dimetro que inva-dia o miocrdio, at ao pericrdio com
6x3x4,5cm. O pulmo apresentava mltiplas pequenas leses redondas e
negras, a maior com 0,9 cm de dimetro. No se ob-servaram outras
leses macroscpicas dignas de registo.
O corao apresentava proliferao de clulas fuso-celulares, com
elevado pleomorfismo e clulas atpicas, com 4 a 5 mitoses/40x, com
disposio estoriforme e fendas com eritrcitos. O pulmo apresentava
mlti-plas metstases com clulas e disposio semelhantes ao observado
no corao, algumas intimamente ade-
rentes aos vasos sanguneos. Todo o tecido adiposo ad-jacente ao
corao apresentava proliferao de clulas neoplsicas.
Concluso: O diagnstico foi de hamangiossarcoma da aurcula
direita com metstases pulmonares, sem envolvimento esplnico.
A case of heart hemangiosarcoma in a dog
Introduction: The consulted literature, referred that hear
hemangiosarcoma, a common condition in Ger-man Shepherd dog,
generally associated with spleen hemangiosarcoma.
The authors report a case of heart hemangiosarcoma without
spleen implication, with clinical parameters and macroscopic
images, associated with lung metastasis.
Material and methods: an heart and lung from an eleven years old
male German Shepherd dog was to the Laboratory of Histology and
Anatomical Pathology, at UTAD. The organs were fixed in 10%
buffered formalin, and processed and stained with haematoxylin and
eosin.
Results: The clinical history indicated absence of vaccination
or dewormed medication, generalized weakness, dyspnoea and shock.
At x-ray the cardiac silhouette is rounded with pleural and
pericardial effu-sion confirmed by ultrasonography.
Euthanasia was requested and at necropsy an abun-dant
hemothorax, and hemopericardium was recorded. The right auricle
presents a papillary villous exofitic proliferation with 3cm of
diameter, that invaded the myocardium until the pericardial wall
with overall dimensions 6x3x4.5cm. The lung presented multiple
black round nodules, the larger with 0.9 cm diameter. There were no
other gross lesions noteworthy.
The histology of the heart lesion revealed fusocel-lular
proliferation with high pleomorphic and atipic cells, with 4-5
mitosis for HPF. The neoplasia had a storiform pattern with clefts
with erythrocytes. The lung had multiple metastases with similar
cells, some of them within vascular structures. All adipose tissue
around the heart had neoplasic cells.
Conclusion: The final diagnosis was hemangiosar-coma of the
right auricle with lung metastasis, without spleen implication.
technology) and the TUNEL technique (1: 2,5; Roche), using the
in situ cell death detection kit.
Results: Canine plasmacytoma revealed moderate positive reaction
with all methods. Canine carcinoma in situ showed moderate positive
reaction with pro-ap-optotic antibodies and more intense with
anti-apoptotic antibody. Dogs thymus showed moderate positive
re-action in cortical thymocytes with pro-apoptotic anti-bodies and
weak in the medullary thymocytes. With the anti-apoptotic antibody,
medullary thymocytes
showed weak reaction and negativity in the cortex. Identical
results were obtained in rabbits thymus, but with stronger
intensity.
Conclusion: The rabbit thymus showed the most specific reaction
of the tissues studied, by which the authors recommended it as a
viable positive control for apoptosis studies.
Acknowledgments: FCT and CI&DETS (PEst-OE/CED/UI4016)
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SUPLEMENTO RPCV (2013) 108 (585-586) 69-91
88
Hrnia de Bexiga associada a Angioleio-miossarcoma Cutneo em
Coelho
Santos S.1*, Patrcio. R.1,2
1 CICV - FMV- ULHT, Lisboa, Portugal2 AlcabidecheVet Clnica
Veterinria, Cascais, Por-tugal
* [email protected]
Introduo: Em Agosto de 2012 um coelho de es-timao com 6 anos de
idade, macho esterilizado foi submetido a cirurgia para extirpao de
uma massa subcutnea ulcerada, localizada na regio inguinal e
associada a uma hrnia de bexiga.
Material e mtodos: Aps a cirurgia a massa foi enviada para
anlise histopatolgica, fixando-se o te-cido em formol tamponado a
10% para colorao com hematoxilina & eosina (H&E).
Realizou-se imunohis-toqumica com -actina (clone, 1A4 DAKO;
1\180)
e desmina (clone DE-R-11, DAKO; 1\50) utilizando intestino e
estmago de co como controlo positivo.
Resultados: A H&E revelou uma neoplasia subcut-nea, no
capsulada e infiltrativa constituda por clulas fusiformes
anaplsicas organizadas em feixes compac-tos e ninhos. As clulas
neoplsicas apresentavam-se intimamente relacionadas com as
estruturas vasculares