xperiment Station - Agricultural Marketing Service

No. 8500103

1lQ)A'lffiI~~M!'IlHlES~ ~RES.E~ SIIJ\'fIIl, ~lUlE:;;

JloribaAgricultural ~xperiment StationtiU1t.er.eas, THERE HAS BEEN PRESENTED TO THE

AN APPLICATION REQUESTING A CERTIFICATE OF PROTECTION FOR AN ALLEGED NOVEL VARIETY

OF SEXUALLY REPRODUCED PLANT, THE NAME AND DESCRIPTION OF WHICH ARE CONTAINED INTHE APPLICATION AND EXHIBITS, A COpy OF WHICH IS HEREUNTO ANNEXED AND MADE A PARTHEREOF, AND THE VARIOUS REQUIREMENTS OF LAW IN SUCH CASES MADE AND PROVIDED HAVEBEEN COMPLIED WITH, AND THE TITLE THERETO IS, FROM THE RECORDS OF THE PLANTVARIETY PROTECTION OFFICE, IN THE APPLICANT(S) INDICATED IN THE SAID COPY, ANDWH EREAS, UPON DUE EXAMINATION MADE, THE SAID APPLICANT(S) IS (ARE) ADJUDGED

TO BE ENTITLED TO A CERTIFICATE OF PLANT VARIETY PROTECTION UNDER THE LAW.NOW, THEREFORE, THIS CERTIFICATE OF PLANT VARIETY PROTECTION IS TO GRANT

UNTO THE SAID APPLICANT(S) AND THE SUCCESSORS, HEIRS OR ASSIGNS OF THE SAID APPLI.CANT(S) FOR THE TERM OF ~hteel1 YEARS FROM THE DATE OF THIS GRANT, SUBJECTTO THE PAYMENT OF THE REQUIRED FEES AND PERIODIC REPLENISHMENT OF VIABLE BASICSEED OF TilE VARIETY IN A PUBLIC REPOSITORY AS PROVIDED BY LAW, THE RIGHT TO EX-LUDE OTHERS FROM SELLING THE VARIETY. OR OFFERING IT FOR SALE, OR REPRODUCING IT,

IMPORTING IT, OR EXPORTING IT, OR USING IT IN PRODUCING A HYBRID OR DIFFERENTTY THEREFROM, TO THE EXTENT PROVIDED BY THE PLANT VARIETY PROTECTION ACTT. 1142, AS AMENDED, 7 U.S.C. 2321 ET SEQ.)

WATERMELON

'Cfu:vctee'

~n ~5tttllOnll Wthcrcof, Y/tame luweuntojetntif k'nd and caU4edthe jeal 0/ tlw ~aut"t!lariety ~r.otediou OOffic.eto be ~-ta:ed

althe Cifay 0/ WMfUl1g:to11, V. C.

tlti4 31.6t ~ 0/ OcA:obvr. inthe JIea?<0/ OfM' ~ one tlwU4a'ndninehunMed and dghty-.6.u.

~"I "/{ ••• ,? g;.,",d",,, tJjiu.#r.,lIu~aIvila"',",,}' ~~ •.••.••

APPROVAL EXPIRES 4.3().86

U.S. DEPARTMENT OF AGRICULTURE FORM APPROVED: OMB NO. 0581-0056AGRICULTURAL MARKETING SERVICE Application Is raqulrad in order to determine,..

" if a plant variety protection certificate Is to

APPLICATION FOR PLANT VARIETY PROTECTION CERTIFICATE be issued (7 l.Ls.c. 2421). Information i.held confidential until certificate i. ISlued

(Instructions on reverse) (7 U.S.C. 2426).-1. NAME OF APPLICANT(S) 2. TEMPORARY DESIGNATION 3. VARIETY NAME

Florida Agricultural Experiment StationUniversity of Florida, IFAS FLA. F83CE Charlee

4. ADDRESS (Street and No. or R.F.D. No., City. State. and Zip Code) 5. PHONE (Include area code) FOR OFFICIAL USE ONLY

Office of the Dean for Research PVPO NUMBER

lO22~McCarty, University of Florida (904) 392-1784 8500103Gainesville FL 326116. GENUS A~.'? SPECIES NAME 7. FAMILY NAME (Botanica/) DATE

0 4/8/85Citrullus lanatus z ------ - - - - -(Thunb.) Matsum. & Nakai Cucurbitaceae ...I TIME

II. 2:30 DA.M. IKJ P.M.B: KIND NAME 9. DATE OF DETERMINATION AMOUNT FOR FILING

Watermelon (1) 20 June 83 0 ~ _1-,- 8..90__w - - - - -(2) 18 July 83 ~ DATE

w 4/8/Ri:;tJ

10. IF THE A.PPLlCA:N~ NAMED IS NOT A "PERSON," GIVE FORM OF ORGANIZATION (Corporationw AMOUNT FOR CERTIFICATEII:

partnershIp, aSSOCIatIon, erc.) , en '. $ ;Loo q:Qww ~------- - - -University of Florida Agricultural Experiment Station II. DjE

L. 101 10 ..z.:l l'j?b11. IF INCORPORATED, GIVE STATE OF INCORPORATION 12. DATEl6F INCORPORATION

13. NAME AND ADDRESS OF APPLICANT REPRESENTATlVE(S), IF ANY, TO SERVE IN THIS APPLICATION AND RECEIVE ALL PAPERS

Dr. J. M. Crall Dr. F. A. Wood, Dean for ResearchUniversity of Florida, IFAS 1022 McCarty Hall, University of FloridaAREC, Leesburg, P.O. Box 388 Gainesville, FL 32611Leesburg, FL 32749 (904) 392-7272 PHONE (Include area code): (904) 392-1784

14. CHECK APPROPRIATE BOX FOR EACH ATTACHMENT SUBMITTEll

a. 0 Exhibit A, Origin and Breeding History of the Variety (See Section 52 of the Plant Variety Protection Act.)

b. ~ Exhibit B, Novelty Statement.c. ~ Exhibit C, Objective Description of Variety (Request form from Plant Variety Protection Office.)

d. ~ Exhibit D, Additional Description of Variety.e. ~ Exhibit E, Statement of the Basis of Applicant's Ownership.15. DOES THE APPLICANT(S) SPECIFY THAT SEED OF THIS VARIETY BE SOLD 8Y VARIETY NAME ONLY AS A CLASS OF CERTIFIED

SEED? (Spe SectiotJ 83(a) of the Plant Variety Protection Act.) [;] Yes (If "Yes." answer items 16 and 17 below) ~ No

16. DOES THE APPLICANT(S) SPECIFY THAT THIS VARIETY BE 17. IF "YES" TO ITEM 16, WHICH CLASSES OF PRODUCTIONLIMITED AS TO NUMBER Of GENERATIONS? BEYOND BREEDER SEFnl'-

len n-L....:...J Yes No U Foundation t:.:..J Registered

lB. DID THE APPLICANT(S) PREVIOUSLY FILE FOR PROTECTION OF THE VARIETY IN THE U.S.? o~..,.....•L-~Certified

Yes (If "Yes," give date)

l

llil No

19. HAS THE VARIETY BEEN RELEASED, OFFERED FOR SALE, OR MARKETED IN THE U.S. OR OTHER COUNTRIES?

O Yas (If "Yes," give namesof countries and dates)

[g] No

20. The appllcant(s) declare(s) that a viable sample of basic seeds of this variety will be furnished with the application and will be re-plenished upon request in accordance with such regulations as may be applicable. .

The undersigned applicant(s) is (are) the owner(s) of this sexually reproduced novel plant variety, and believe(s) that the variety isdistinct, uniform, and stable as required in Section 41, and is entitled to protection under the provisions of Section 42 of the PlantVariety ~rotection Act.

Applicant(s) is (arc) informed that false representation herein can jeopardize protection and result in penalties.

SIGN URE OF APPLIC~NT I DATE

27YJ1 Feb. 85

1

INSTRUCTIONS-:"-:r:"'CjX3 _'A.VOC::o",-

General: Send lin original copy of the application and exhibits, at least 2,500 viable seeds, and $1,800 fee ($200 filing fee and $1,600ex'!mlnarlQn fee) to U.S. Department of Agriculture, Agricultural Marketing Service, Plant Variety Protection Office, NationalAgric~lltural Library au IIding, Beltsville, Maryland 20705. (See section 180,115 of the Regulations and Rules of Practice.) Retainone coPY for your files. All items on the face of the form are self-explanatory unless noted below.

9 Give the date the applicant determined that he had a new variety based on (1) the definition in section 41 (a)bf the Act and (2) the date a decision was made to increase the seed.

14a _Give: (1) the genealogy, including public and commercial varieties, lines, or clones used, and the breeding::method; (2) the details of subsequent stages of selection and multiplication; (3) the type and frequency ofvarjants during reproduction and multiplication and state how these variants may be identified and (4)evidence of uniformity,and stability.

14b Give a summary statement of the variety's novelty. Clearly state how this novel variety may be distinguishedfrom all other varieties in the same crop. If the new variety most closely resembles one or a group of relatedvarieties: (1) identify these varieties and state all differences objectively; (2) attach statistical data forcharacters ex'pressed numerically and demonstrate that these are clear differences; and (3) submit, ifhelpful, seed and plant specimens or photographs of seed and plant comparisons clearly indicating novelty.

14c Fill in the Exhibit C, Objective Description form, for all characteristics for which you have adequate data.

14d Describe any additional characteristics that are not describe:d, or whose description cannot be accuratelyconveyed in Exhibit C. Use comparative varieties as is necessar'l to reveal more accurately the descriptionof characteristics that are difficult to describe, such as plant habit, plant color, disease resistance, etc.

14e Section 52(4) of the Plant Variety Protection Act requires applicants to furnish a statement of the basis of theapplicant's ownership. The applicant may be the actual breeder, the employer of the breeder, the owner throughpurchase or inheritance, etc.

\

15 If "Yes" is specified (seed of this variety be sold by variety name only as a class of certified seed) theapplicant may l'iQI reverse his affirmative decision after the variety has either been sold and so labeled,his decision published, or the certificate has been issued. However, if the applicant specified "No," he maychange his choice. (See section 180.16 of the Regulations and Rules of Practice.)

19 See section 41 (i, j) and 42 of the Plant Variety Protection Act and section 180.7 of the Regulations andRules of Practice for eligibility requirements.

FORM WA.470 (7-84) (Reverse)

14a. Exhibit A, Origin and Breeding History 850010.3

'Charlee' originated from a cross made in 1962

between the highly Fusarium-wilt-resistant Texas W5 and

'Charleston Gray' (Figure 1). After several years of

selfing, a cross was made in 1971 with 'Calhoun Gray',

which is also highly wilt resistant. Following this cross

there were four backcrosses with USDA 51-27 ('Charleston

Gray') and seven selfings, the last four of which followed

the final cross with USDA 51-27 in 1978. No variants from

the Charleston Gray type were noted after this final back

cross. An isolated increase (F83 CE) of the final self

pollination (82 M21-5) was made in the fall of 1983. Seed

from this planting was furnished to the Florida Foundation

Seed Producers, Inc. for distribution to seedsmen as'Charlee'.

2

,

1 Year2

3 19624 19635 19646 19657 19668 19679 1968

10 196911 197012 197113 197414 197515 1976 spring16 1976 fall17 1977 spring18 1977 fall19 197820 197921 198022 198123 198224 19832526

850010.]Progeny designation

Texas W5 x Charleston Gray63-7-2 self64 NB41-7-01 self (Long Watson)65 K124-6 self66 1"1-1 self67 H7-3 self68 B25-3 self (Long Watson)69 MIO-3 self70 F19-6 open71 c6-5 (Long Watson) x Calhoun Gray

72 MD6-1 self (Long Watson)75 020-6 x USDA 51-27 (Charleston Gray)76 MJ~-3 selfF76C53-4 x USDA 51-2777 K33-4 selfF77125-1 x USDA 51-2778 T27-4 x USDA 51-2779 G36-1 self80 NB5-2 self81 J7-6 self82 M21-5 selfF83CE (isolated increase of 82 M21-5 self)

27 Figure 1. Pedigree of CharleeJ.

".

8500103l4b. Exhibit 0, Novelty Statement

This variety most nearly resembles Charleston Gray

and Charleston Gray #5. It is similar to but

distinguishable from Calhoun Gray by the generally lower

fruit quality factors of the latter (Table 1). Charlee

has the high-level resistance to Fusarium wilt

characteristic of Calhoun Gray as compared with the

moderate resistance of Charleston Gray (Table 2). It is

resistant to anthracnose (race 1), as is Charleston Gray.

In contrast Calhoun Gray is susceptible to anthracnose and

Charleston Gray #5 segregates for anthracnose resistance.

~~ ~~.Cv~.,a--~,-~116

TL-v~~~~'~~ G-~. (

850010.3

Table 1. Marketable yields and fruit character ratings for cultivars in replicated trials,Leesburg, 1983.

Cultivar

Yieldper acre

No. fruits Cwt

Meanfruit

wt (lb)

Fruit charactersFlesh

ColorY TextureY FlavorYQuality

xRating

Trial A

Calhoun Gray 2291 z 478 a 21 b 3.8 a 3.8 a 2.7 a 3.3 a 3.4 aa

Charlee 1803 b 429 ab 24 b 3.8 a 4.5 a 4.7 a 4.2 a 4.3 a

Jubilee 1481 b 399 ab 27 a 3.5 a 4.2 a 3.8 a 3.3 a 3.7 a

Char. Gray 1681 b 382 b 23 b 3.5 a 4.5 a 3.5 a 3.5 a 3.8 a

Trial B

Charlee 1760 a 449 a 26 a 4.5 a 5.0 a 5.3 a 5.0 a 5.0 a

Jubilee 1612 a 432 a 27 a 3.3 a 4.5 a 3.3 b 4.8 b 3.7 b

Char. Gray 1742 a 426 a 24 a 4.0 a 4.8 a 3.5 b 4.3 b 4.2 ab

zMean separation in columns by Duncan's multiple range test, 5% level.

YScale for grading shape conformation, flesh color, flesh texture, and flesh flavor:o = poor to 6 = excellent. Shape ratings are based on conformation to a desirable type.

xQuality rating is mean of all other fruit character ratings.

s

. '

Table 2.

850010.3Resistance to Fusarium wilt in Charlee and othercultivars.

Percent wiltCultivar Greenhouse Z FieldY

Charlee a 24

Calhoun Gray a 12

Charleston Gray 33 54

New Hampshire 100 100

zSeedling wilt after approximately three weeks infumigated soil infested at 600 propagules per gram withsoil inoculum of Fusarium oxysporum f. sp. niveum.

YTotal wilt, including both seedling and mature plants,determined by visual inspection with removal ofcompletely wilted plants, on a weekly (after 12 weeks) ormore frequent (first 12 weeks) schedule. Soilinfestation with causal organism of watermelon Fusariumwilt was at a much higher level than ordinarily would beencountered in commercial production fields.

,1

-2-

Table A. Comparative seedling wilt in 1972 greenhouse test (GH 72-4) for wiltresistance in several watennelon cultivars.

Final percent wiltIsolate number

Cultivar 60-3A 62-21 63-38A Mean

New Hampshire Midget 100 100 100 100Texas W5 0 0 0 0Srnokylee 0 0 0 0Charleston Gray #133 57 62 53 57Charleston Gray 69 89 56 71Crimson Sweet 51 77 50 59

Table B. Comparative seedling wilt in several greenhouse tests for wiltresistance in various watennelon cultivars.

Final percent wiltGH test number

83- 83-Cultivar 83-2 2RR(A) 2RR(B) 84-3A 84-3B 85-2A 85-28 86-13 Mean

New Hampshire 100 100 100 100 100 100 100 96 99Midget

calhoun Gray 0 0 0 0 0 0 0 0 0Charlee 0 0 0 0 0 0 0 0 0Charleston Elite 0 0Charleston Gray 86 67 80 83 87 90 93 94 85Charleston 18 29 30 40 36 17 20 27

Gray #5

850010.3

14d. Exhibit 0, Additional Description of Charlee

Plants of 'Charlee' are vigorous and prolific,

comparing favorably with 'Charleston Gray' in these

characteristics. Fruits of both cultivars ripen in early

mid-season.

Fruits of 'Charlee' are similar to those of

'Charleston Gray #5' in size, shape, and weight (Table 3).

They are remarkably uniform in both external and internal

appearance (Fig. 2). Flesh color is an attractive red,

but not as intense as that of 'Oixielee'. Sugar content

of the juice (percent soluble solids) is slightly higher

than that of 'Charleston Gray #5' (Table 3). Rind is a ~

solid gray-green with a faint net and rind thickness

measured 0.75 inch ,compared with 0.50 - 0.75 inch for

'Charleston Gray #5'. The rind is hard and tough and well

suited for shipping. Seeds are black, stippled, and

medium large 'Charlee' is resistant to anthracnose (race

1) and Fusarium wilt. Its wilt resistance is the same

"high-type" characteristic of 'Calhoun Gray' and severalh I. 1ot er cu tlvars. In greenhouse tests for seedling wilt

lCral1 J.M. 1981. Identification of the sources of highlevel resistance to Fusarium wilt in watermelon.Phytopathology 71:869 (Abstr.).

10

. I

850010.3

Charlee Charleston Grav #519-30 20-3023 24

17.6 18.18.20 8.302.15 2.18.75 .50-.75

10.8 10.5red red

1 Table 3. Comparison of ripe Charlee and Charleston Gray #5

2 fruits, 1984.3

4 Characteristic

5 Range of weights (pounds)

(pounds)

(inches)

(inches)

(L: D)

(inches)

(percent)

6 Mean weight

7 Length

8 Diameter

9 Shape ratio

10 Rind thickness

11 Soluble solids

12 Flesh color

1314151617181920212223

24

2526

27

11

INSTRUCTIONS: See Reversrt.

• I . FORM APPR()VED - OMB NO. 0581-0055

UNITED STATES DEPARTMENT OF AGRicuLtURE EXHIBIT CAGRICUL. TURAL. MARKETING SERVICE {WaterrIWlotII}

LIVESTOCK, POULTRY, GRAIN & SEED DIVISIONBELTSVILLE, MARYLAND 20706

OBJECTIVE DESCRIPTION OF VARIETYWATERMELON (CITRULLUS LANA TUS) .

FOR OFFICIAL USE ONLYPVPO NUMBER

A ADESIGNATION

R EMPORARY

8500103rt~ffd~~gt~~ ural Experiment StationUniversit of Florida IFASADDRESS (Street and No. or R.F.D. No., City, .StBte, and ZIP Code)Office of Dean for Research1022 McCarty HallUniversity of Florida

?

Place the appropriate number that describes the varietal character of this variety in the boxes below.

Place. a zero in first box (e.g. I 0 I 81 91 or EQI!I ) when number is either 99 or less or 9 or less.

1. TYPE:

1 - OBLONG 2 = ROUND LARGE 3 = ROUND SMALL (icebox)

2. AREA OF BEST ADAPTATION:

1 = SOUTH 2 NORTHEAST/NORTHCENTRAL 3 = SOUTHWEST 4 = MOST AREAS

3. EMERGENCE TO ANTHESIS:

CD NO. OF DAYS EARLIER THAN •••••.B} = CHARLESTON GREY

'm NO. OF DAYS LATER THAN •.•.•••••• 2 = OTHER (Specify)

4. POLLINATION TO MATURITY:

NO. OF DAYS EARLIER THAN •.•••• [2].} 21= CHARLESTON GREY

NO. OF DAYS LA TER THAN •••.•••.•. CD - OTHER (Specify) -------------------

5. PLOIDY:

DIPLOID 2 TETRAPLOID 3 = TRIPLOID

2 - ANDROMONOECIOUS

6. PLANTo Cotyledon: 1 = FLAT 2 = FOLDED

Number of flowers per plant at first fruit set:CD STAMINATE CD PISTILLATE

[2] 1 = MONOECIOUS

~ PERFECT D NO. OF MAIN STEMSAT CROWN

CM. VINE LENGTH +- NO. OF INTERNODES (At last harvest)

IT] MM. DIAMETER AT SECOND NODE

7. STEM:

1 = ROUND

1 = GLABROUS

2 = ANGULAR

2 = SCABROUS 3 = PUBESCENT 4 = BRISTLED

8. LEAF:

1 - LONGER THAN WIDE 2 = LENGTH-WIDTH EQUAL3 = WIDER THAN LONG1 - OVATE 2 = OBOVATE

Dorsal Surface: .} 1

= SMOOTH

Ventral Surface:"

3 = ROUND

2 = PUBESCENT

Color:1 = LI\:;HT GREEN

3 - MEDIUM GREEN

2 = GRAY GREEN

4 = DARK GREEN

9. FLOWER (At first fruit set):

OJ Staminate: CM. ACROSS IT] Pmect: .CM. ACROSS 0 Cole.:1 = LEMON YELLOW2 = YELLOW 3 = .ORANGE

FORM LPGS-47Q-19 (1-81) (Formerly GR-470.19 (1-16.73), which may be used.)

E;'"5UO10_3--- _.10. MATURE FRUIT:

2 - OVAL

2 - SLIGHTLY GROOVED

CM. DIAMETE"AT MIDSECTION

3 - MEDIUM GREEN(SugarBaby)

CM. LONG

INDEX - LENGTH + DIAMETER X 10

3 - DEEPLY GROOVED

2 - LIGHT GREEN (Charleston Grey)

5 - OTHER(Specify) _

3 - MOTTLE/NET2 - STRIPE

3 - CYLINDRICAL

YELLOW GREEN f"-t K",.JDARK GREEN (FlorltM (JIMtJ

1 - SMOOTH

Primary Color: } 1 -

Secondary Color: 4-

~ 1 - ROUND

~ KG. AVERAGE WEIGHT

o~ Color: 1 - SOLID (One color)

oD11. RIND:o 1 = TENDER 2 = BRITTLE 3. = TOUGH rn THICKNESS MM. BLOSSOM END

~ THICKNESS MM. SIDEa

% CHECK VARIETY (Specify) Charleston Gray 115

12. FLESH:o 1 - CRISP 2 - SOFT

~

COlor: 1 - YELLOW 2 = ORANGE 3 = PINK

11 REFRACTOMETER % SOLUBLE SOLIDS OF JUICE(Center of fruit)

% HOLLOW HEART

4 =

1 - COARSE.FIBROUS

RED 5 = DARK RED

dltJIIIOl % PLACENTAL~ SEPARATION

2 = FINE.LITTLE FIBER

% TRANSVERSECRACK

2 = WHITE.TAN TIPPED 3 = WHITE.PINK TIPPED- .7 = DARK BROWN 8 = DARK BROWN MOTTLED 9

14. DISEASE RESISTANCE: (0 = Untested, 1. = Susceptible, 2 = Resistant)

D ANTHRACNOSE (Race 1 )[TI DOWNY MILDEW [2]~ SQUASH MOSAIC [U WATERMELON IT]MOSAIC CUCUMBER MOSAIC

GUMMY STEM BLIGHT

1.95rn MM.THICK

ITJJ NO. SEEDPER FRUIT

4 - TAN 6 • GREEN

BLACK 10 - Mm['XXElJ:BLACKstippled

POWDERY MILDEW

FUSARIUM WILT

~.7~ MM. WIDE

rn GM. PER 1000 SEED

1 - WHITE. • - "ED

12.1MM. LONG

INDEX + LENGTH + WIDTH X 10

Color:

13. SEED:

D OTHER (Specify) ~------

15. OTHER RESISTANCE: (0 = Untested, 1 = Susceptible, 2 = Resistant)

o SUNBURN o ROOT KNOT o OTHER (Specify) _

Charleston GraCharleston GraCharleston Gra

16. NAME A VARIETY THAT MOST CLOSELY RESEMBLES THAT SUBMITTED:

Charleston Gra Fruitshae

Charleston Gra Rind color

Charleston Gra Flesh uali

REFERENCH:1. Prey~L J. 1966. plant Breedins - Symposium. 1 ed. Iowa State University PrelL2. W••.• , G. W. and McCollum, J. P. 1968 .. Producins Vesetab1e Crops .. Int~state Printers & PublUbers, Inc. Da;nYil1e,Illinois.-3. Whitak., T. W. aftcI Da-ris, G. N. 1962. Cucurbits. hiterscience Publishers, Inc. New York.4. Nickerson's or any recognized color fan should be used to_determine the p~t colors of the described variety.

9

-FORM LPG,3.470.19 (1-81) Reverse

1

2

3

4

5

6

7

8

9

1011

1213141516171819202122

23242526 Figure 2 •

27Fruit of Charlee from 20 x 25 ft. plot in

replicated trial, Leesburg, 1983.

850010.3

i2

" .

8500103resistance, 'Charlee' was superior to all other cultivars

tested. In a heavily infested field area, percentage

wilt of 'Charlee' was less than half that of 'Charleston

Gray' (Table 2).

'Charlee' was grown in replicated trials at AREC Leesburg

in 1983 and 1984, and in grower trials at three locations

in 1984.

Yield differences between 'Charlee' and other entries

were not statistically significant in two separate

replicated trials at Leesburg in 1983 (Table 1), but

'Charlee' yield was higher than those of 'Jubilee' and

'Charleston Gray' in both trials. In one 1984 replicated

trial at Leesburg (Table 4), 'Charlee', 'Charleston Gray

#5', and 'Crimson Sweet' yields were not significantly

different, but all three yielded significantly more than

'Jubilee'. In another 1984 replicated trial, differences

between entri~s were not significant, but yields of

'Jubilee', 'Charlee', and 'Charleston Gray #5' were

somewhat higher t~an those" of 'Charleston Gray;.

In both years' tests (Tables 1 and 4), fruit character

ratings indicated that fruits of 'Charlee' were moreuniform in shape and of higher quality in flesh color,

texture, and flavor than those of 'Calhoun Gray',

'Charleston Gray', 'Charleston Gray #5', 'Crimson Sweet',

10\

Table 4. Marketable yields and fruit character ratings for cultivars in replicatedtrials, Leesburg, 1984.

Fruit charactersYield Mean Flesh

per acre fruit SolubleCultivar No. fruits Cwt (J.b) SllapeY TextureY FlavorY Holidswt

(%)

Trial A

Chat: Gray tiS 2723 Z 537 a 20 a 3.1 a 3.3 b 2.7 c 10.6 aaCharlee 2396 a 503 a 21 a 3.9 a 4.6 a 4.3 a 10.8 aCrim. Sweet 2396 a 494 a 21 a 2.5 a 3.8 ab 3.3 bc 10.3 aJubilee 1307 a 285 b 22 a 3.0 a 4.4 a 3.7 ab 10.8 aSugar Baby 3409 a 281 b 8 b 2.9 a 3.0 b 2.8 bc 10.9 a

Trial B

Jubilee 1725 a 405 a 24 a 3.0 a 3.4 ab 3.3 a 10.3 aCharlee 1856 a 396 a 22 ab 4.3 a 4.4 a 3.5 a 10.5 aChar. Gray tiS 1986 a 381 a 19 b 3.4 a 3.0 ab 2.8 a 10.1 aChar. Gray 1525 a 335 a 22 ah 4.0 a 2.3 b 3.1 a 10.5 a

ZMean separation in columns by Duncan's multiple range test, 5% level.

YScale for grading shape conformation, flesh texture, and flesh flavor: 0 = poor to6 = excellent. Shape ratings are based on conformation to a desirable type.

.If

8500103'Jubilee', and 'Sugar Baby'. Soluble solids content of

'Charlee' was comparable or slightly higher than those of

all other entries.

Tests of this line with growers have been limited, but

tests comparing 'Charlee' with 'Charleston Gray' were

made in 1984 in Collier, Lake, and Levy Counties.

'Charlee' was the best of six lines in the Collier County

test. It was early and prolific; the fruit had excellent•shape and internal quality equal to or better than that

of all other lines in the test. In both Lake and Levy

County tests, 'Charlee' performed well. In the Levy

County test wilt resistance was superior to that of

commercial 'Charleston Gray'.

is

850010314e. Exhibit E, Statement on the Basis of Applicants

Ownership

Charlee watermelon was developed by the Institute of

Food and Agricultural Sciences at the Agricultural

Research and Education Center., Leesburg, Florida. It was

tested as Florida line F83 CE and released as such to the

Florida Foundation Seed Producers, Inc., P.O. Box 309,

Greenwood, FL 32443.

Seed stocks of Char lee may be sold only by company

members of Florida Foundation Seed Producers, Inc. who

have signed a Production and Distribution Agreement with

the authorized agent of the corporation.

i'. . ~