www.optocell.de The CuBiAn XC – A universal tool for your bioprocess analysis Sandra Koptik Alexander Hähnel CellD 20bis, rue du Chapitre F-30150 ROQUEMAURE Tel : +33 (0)4 66 82 82 60 Fax : +33 (0)4 66 90 21 10 Email : contact- [email protected]www.celld.com
23
Embed
Www.optocell.de The CuBiAn XC – A universal tool for your bioprocess analysis Sandra Koptik Alexander Hähnel CellD 20bis, rue du Chapitre F-30150 ROQUEMAURE.
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
www.optocell.de
The CuBiAn XC – A universal tool for your bioprocess analysis
The Reagent and Sample Container Unit has space for 20 assay bottles and 10 samples
Simple, automated handling or reagents and samplesSa
mpl
e &
Rea
gent
Han
dlin
g
4
www.optocell.de
Samples and reagents automatically pipetted into disposable cuvettes in the Incubation Reaction Unit (IRU)
Reactions constantly incubated at 37 °C
Rapid delivery of results
Each reaction is mixed thoroughly
The cuvettes will be uploaded, checked before use and discarded by the automated robot arm
Each reaction takes exactly 10 minutes
Assays measured every 40 seconds
Up to 90 results/hour possible (360 with ISE unit)
Samples can be run in replicates (2-40)
The measurement workflowSa
mpl
e &
Rea
gent
Han
dlin
g
5
www.optocell.de
The automated pipetting system for reagents and samples guarantees for a high reproducibility and accuracy
The reagent and sample syringes work with a cv of less than 1%, and 2% respectively
The accuracy of pipetting can be checked at any time by the user via (SPT+ RPT (Sample and Reagent Precision Tests; part of the CuBiAn®
SST concept)
Liquid handlingAc
cura
cy a
nd p
reci
sion
6
www.optocell.de
Two multi- (universal) calibrators create calibration curves for the majority of the assays (Lactate, Glucose, Ammonia, Glutamate, Inorganic Phosphate, Iron, Mg, Cholesterol,...)
Separate Calibrators for Glutamine and IgGs
Two or three levels of controls (low, medium, high) available to check the system’s precision of the specific assay
Calibrator and Control liquidsAc
cura
cy a
nd p
reci
sion
7
www.optocell.de
Calibration graphsAc
cura
cy a
nd p
reci
sion
8
www.optocell.de
Control status documented in quality graphs
(Levey-Jennings plots)
Accu
racy
and
pre
cisi
on
9
www.optocell.de
Customizable warning scenariosAc
cura
cy a
nd p
reci
sion
10
www.optocell.de
(hs) IgG 10.0 – 500 mg/L
(hc) IgG 2.8 – 27.55 g/ L
Total Protein 0.04 – 2.25 g / L
Glucose 0.09 - 6.3 g/L
Lactate 0.05 – 8.0 g/L
Ammonium 0.05 – 9.0 mM
Glutamine 0.06 – 10 mM
Glutamate 0.12 – 10 mM
Inorganic Phosphor 0.13 – 10.32 mM
LDH (L->P; P->L) 13 – 2800 U/L
Na+ 80-180 mM
K+ 1.5 - 10 mM
CL- 60-140 mM
Reruns and pre-dilutions allow to enlargethe measurement ranges displayed above
ISE unit
Reagents
Extract of current assays and rangesTh
e as
say
portf
olio
11
www.optocell.de
Acetic Acid (launched; 0.05 – 1.25 g/L)
Magnesium (launched; 0.2 -3.27 mMol/L)
Cholesterol (launched; 0.87 – 16.6 mMol/L)
Iron (launched; 2 – 198 µMol/L)
Glycerol (final tests; 0.016 -2.5 mMol/L)
Galactose (under development: 0.05 – 7 g/L)
Recently launched, in final tests, under developmentTh
Validation documents are available (Verification documents including IQ, OQ); PQ on demandExperienced validation consulting and support service by OPTOCELL technologyDocuments developed in close cooperation with customers
ValidationTh
e Va
lidati
on P
acka
ge
17
www.optocell.de
Membrane based (electrochemical) systems
CuBiAn® photometric & immuno-turbidimetric systems
Expandability Limited Nearly unlimited and customizable
Necessary Frequency of Calibration
Very high Once per lot of reagents
Reproducibility of Results Low High
Immuno-turbidimetry Not possible Implemented
On-line capability Limited due to chemical sterilization
BayChromat® CellCount + CuBiAn®XC with steam sterilized sample valve
Rate analysis Not possible Implemented
Phot
omet
ric v
s. M
embr
ane
base
d an
alys
ers
Comparison to membrane based analysers
18
www.optocell.de
Membrane based electrochemical (biosensors) systems
CuBiAn® photometric and immunoturbidimetric Systems
Comparison to membrane based analysersPh
otom
etric
vs.
Mem
bran
e ba
sed
anal
yser
s
19
www.optocell.de
Comparison to membrane based analysersPh
otom
etric
vs.
Mem
bran
e ba
sed
anal
yser
s
20
www.optocell.de
The CuBiAn® XC can be completely integrated into a fully automated bioreactor environment without any technical hardware changes
The remote access software (developed together with Bayer TechnologyServices, Germany) can be easily upgraded to an CuBiAn® XC
The steam-sterilizable automatic sample drawing system of the BayChromat ® Cell Count has shown its high stability in several contamination-free cultivations, lasting over more than 180 days
The BayChromat ® control software allows for the integration of additionalanalysing devices
Auto
mati
on c
apab
ility The automation capability of the CuBiAn XC
21
www.optocell.de
The STAT port
The BayChromat® Cell Count by:Bayer Technology Services,
Leverkusen, Germany
Auto
mati
on c
apab
ility Comparison to membrane based analysers
22
www.optocell.de
Out
look
– C
uBiA
n Fa
mily
Throughput: 270 tests/hour (max. 404 tests/hour with ISE High precision pipettor und reagent pipettor Environment-friendly semi-disposable resin cuvettes Refrigerated Reagent Container Unit Optional built-in ISE module available Optional built-in degaser module available