WP6 presentation Quality of oats : protein and oil contents Micronutriments and avenins AVEQ meeting BUCHAREST 19-21/10/2010.

WP6 presentation

Quality of oats : protein and oil contentsMicronutriments and avenins

AVEQ meeting BUCHAREST 19-21/10/2010

Partners for this workpackage :

AVEQ meeting BUCHAREST 19-21/10/2010

France : Jean Koenig INRA Clermont-FerrandGérard Branlard INRA Clermont-FerrandAudrey Didier INRA Clermont-FerrandExternal service : Vetagro sup Agnès Piquet

Estonia : Külli Annamaa Jogeva Plant Breeding Institute

Romania : Danela Murariu Genebank Suceava Manuela Ibanescu Genebank Suceava

Preliminary work to analyses :

Dehulling of seedsDone by Alf Seplitis (Partner 2) and Mathias Hermann (Partner 3)

Hand dehulling has and will be done by IHAR Radzikov and PBI (Estonia) by hand which is a quite long workThanks to them

Milling has been done by laboratories carrying out NIRS analyses (problems of sticky flour..... )

AVEQ meeting BUCHAREST 19-21/10/2010

AVEQ meeting BUCHAREST 19-21/10/2010

Near Infrared Spectra analysis (NIRS)

On samples 2008 , have been realized for 6 locations : BUL, EST, FRA, ITA, ROM, POL, and on samples 2009 are on the way.

Calibration by Kjeldahl (proteins) and (Fat) and Calibration verification have been done on 2008 samples. Calibration values will be entered in the NIRS software to produce protein and fat values.

Protein content

First results from calibration by Kjeldahl method show a wide range of variation : within locations and looking at all locations

AVEQ meeting BUCHAREST 19-21/10/2010

Micronutriments• Have been realized on 122 (100 selected by

PCA + 2 X 11 Standards) samples from 2008 (mineral micronutriments)on samples from french harvest. But results were not produced yet by partner (external)

• Samples have been chosen on spectra from NIRS analysis (PCA) choosing more diverse accessions

• Will be done on samples from France 2009 in the next months

AVEQ meeting BUCHAREST 19-21/10/2010

AVEQ meeting BUCHAREST 19-21/10/2010

Results for avenins patterns

Avenin identification

•Avenin extraction

•Allele identification for the 3 loci: Ave1, Ave2 et Ave3

•1D electrophoresis Acide-Page

Method

Identification of alleles of Ave1 locus

•5 alleles

Identification of alleles of Ave2 locus

•6 alleles

Identification of alleles of Ave3 locus

•10 alleles

•22.4 % of accessions were heterogenous

•Genetic heterogeneity within accession

•Grain mixture within accession

Homogeneity (purety) of the Avena sativa patterns as revealed through avenin analysis

•80% old accessions

•20% modern accessions

Locus identifié EffectifPourcentage

(N=543)

Ave1 30 5.5

Ave2 22 4.0

Ave3 53 9.8

Ave1 + Ave2 29 5.4

Ave1 + Ave2 + Ave3 152 28.0

Ave1 + Ave3 76 14.0

Ave2 + Ave3 99 18.2

Aucun 82 15.1

Identification of avenin alleles in A. sativa

Alleles repartition at locus Ave1 ( N=300, 2008)

Alleles repartition at locus Ave2 ( N=300, 2008)

Alleles repartition at locus Ave3 ( N=300, 2008)

Thanks for your attention

AVEQ meeting BUCHAREST 19-21/10/2010

Deliverable number

Planned completion Description

Status

Achieved Revised completion

Who

D6.1 Month 36 Protein and fat data from 1st field experiment year reported Month 46 P5

D6.2 Month 40 Database with 600 Avenin patternsX P5

D6.3 Month 44 Database with 6600 NIRS spectraMonth 48 P1

D6.4 Month 48 Protein and fat data from 2nd field experiment year reportedMonth 48 P11

D6.5 Month 48 All analysis results available in EADBMonth 48 P1

Milestone number

Planned completion Description

Status

Achieved Revised completion

Who

X P5

X P5

X P5

X P11, P9

X P5

Month 48 P11

Month 48 P9,P11

Month 47 P5

Overview of deliverables WP6