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WP5 - MICROBIAL METABOLIC ACTIVITIESCNRS DR 12 - LMM
BACTERIAL ACTIVITIES
* STRICT ANAEROBIC ACTIVITIES* Methane Production (MP)* Sulfate Reduction (SR)
* BACTERIAL PRODUCTION & ACTIVITY* Bacterial Production (BP)* Glutamate & Glucose Uptake* Enzymatic Activities
Contract EVK3-2000-00042
D. MARTY, J. GARCIN, C. TAMBURINI, A. BIANCHI
BIODEEP Meeting VIII VENEZIA 13-15 October 2003
MICROBIAL ACTIVITIESBIODEEP CRUISES 2001 & 2002
L’ATALANTE, BANNOCK, DISCOVERY, URANIA
* BRINES* INTERFACE (Seawater-Brine)
Seawater, Upper & Lower Interface (2001)
Along Interface (2002)* BRINES
Brines, Upper Brine, Heavy Brine (Urania)
* MUD PIT (Urania)
* SEDIMENT * OVERLAYING BRINE (or BOTTOM BRINE) * SEDIMENT (Levels A, B, C)
STRICT ANAEROBIC ACTIVITIES
* METHANE PRODUCTION (MP) CH4 produced (µmol/L)
* NATURAL MP & POTENTIAL MP * No Amendment & Pool of Substrates
MP = METHANE PRODUCTION µmol CH4 produced per liter
MP Natural : [CH4] µM
0 200 400 600 800 1000
MPR Natural : [CH4] µM/d
MPR = METHANE PRODUCTION RATE µmol CH4 produced per liter per day
0 50 100 150 200
BRINE
BOTTOMBRINE
SED-A
SED-B
SED-C
BRINE
BOTTOMBRINE
SED-A
SED-B
SED-C
INTER
FA
CE
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
METHANE PRODUCTION
ATALANTE
BANNOCK
DISCOVERY
0 10 20 30 40 50 60
MP Natural : [CH4] µM MPR Natural : [CH4] µM/d
0 5 10 15 20 25 30 35
MP = METHANE PRODUCTION µmol CH4 produced per liter
MPR = METHANE PRODUCTION RATE µmol CH4 produced per liter per day
BRINE
BOTTOMBRINE
SED-A
SED-B
SED-C
BRINE
BOTTOMBRINE
SED-A
SED-B
SED-C
INTER
FA
CE
URANIA BASINMETHANE PRODUCTION ± INHIBITOR OF SR
MPR in the presence or absence of Molybdate
(inhibitor of SR)
MPR = METHANE PRODUCTION RATE µmol CH4 produced per liter per day
Brine Brine MudPit Mudpit Sed-B Sed-B +MOB +MOB +MOB x 17 x 1.5 x 5.7
MPn
at:
CH
4 µ
M/l
0
50
100
150
200
250
300 without MOB
with MOB
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
SULFATE REDUCTION
0 25 50 75 100 125 150
SRR : H2S mM/d
ATALANTE
BANNOCK
DISCOVERY
URANIA
SRR : H2S mM/d
0 50 100 150 200 250 300 350
SRR = SULFATE REDUCTION RATE µmol H2S produced per liter per day
BRINE
BOTTOMBRINE
SED-A
SED-B
SED-C
INTER
FA
CE
INTERFACE
BRINE
BOTTOM BRINE
SEDIMENT-A
SEDIMENT-B
AMINO-PEPTIDASE ACTIVITY nmol produced per liter per hour
pmol/l/h
0.1 1 10 100 1000 10000 100000
SW 3 000 mATALANTE
BANNOCK
DISCOVERY
URANIA
OXIC
PHOSPHATASE ACTIVITY nmol produced per liter per hour
INTERFACE
BRINE
BOTTOM BRINE
SEDIMENT-A
SEDIMENT-B
pmol/l/h
0.1 1 10 100 1000 10000 100000
SW 3 000 m
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
ENZYMATIC ACTIVITIES
GLUCOSE UPTAKEpmol per liter per hour
SW
BRINE
BOTTOM BRINE
SEDIMENT-A
SEDIMENT-B
pmol/l/h1 10 100 1000 10000 100000
Ligurian Sea 2000 m
ATALANTE
BANNOCK
DISCOVERY
URANIA
SW
GLUTAMATE UPTAKEpmol per liter per hour
SW
INTERFACE
BRINE
BOTTOM BRINE
SEDIMENT-A
SEDIMENT-B
pmol/l/h0.1 1 10 100 100010000 100000 1000000
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
GLUTAMATE & GLUCOSE UPTAKE
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
BACTERIAL PRODUCTION
BACTERIAL PRODUCTIONng C per liter per day
SW ATALANTE BANNOCK DISCOVERY URANIA0
0.2
0.4
0.6
0.8
Interface
Brine
SW 3000 m BACTERIAL PRODUCTION 2001
ng C per liter per day
DC = Samples DecompressedHP = Samples Ambient Pressure
SW ATALANTE BANNOCK DISCOVERY URANIA0
0.5
1
1.5
2Brine-DC
Brine-HP
L'ATALANTE, BANNOCK, DISCOVERY & URANIA
EFFECT OF PRESSURE CONDITIONS ON BACTERIAL ACTIVITIES
AA = Aminopeptidase Activity
PA = Phophatase Activity
GLUT = Glutamate Uptake
BACTERIAL ACTIVITIESDC = Samples Decompressed
HP = Samples Ambient Pressure
DC
HP
0
10
20
3056.30
SEAWATER ATALANTE BANNOCK
AA GLUT AA AA PA GLUT
SEAWATER ATALANTE BANNOCK
CONCLUSIONS
* PRESENCE OF ACTIVE ARCHAEBACTERIA & BACTERIA IN THE DHABs
* INTERFACE* BRINES* SEDIMENT
* BACTERIA ADAPTED TO IN SITU CONDITIONS (HIGH PRESSURE, HIGH SALINITY, ANOXIA...) AS ACTIVITY IS HIGHER UNDER AMBIENT CONDITIONS THAN IN DECOMPRESSED SAMPLES