1. Skin Preparation PDI ® Chlorascrub™ Swabstick • Afte rloc ation ofthe vein,vigorousl yscr ubthe veni puncture site with PDI ® Chlorascrub™Swabstickfor30seconds. 1 • All ow the si te to air dry be for e v eni punc tur e. • Do not re-palpate the vein. 2.BottlePreparation • Inspec teac hbloodcult urebo ttle befor euse toe nsure inte grit y ofbottleandsensoronbottomofbottleisintact. Thesensor isnormallyauniformgrayish-gree ncolorandayellowcolor wouldindicatecontaminationofthebroth.Discardanybottle foundtobedamagedorwithasensorthatis yellow. • Remove protecti ve ip top ov ercap. • NOTE :The sept umis nots teril eand mustb edis infec ted. • Cle ans e t he septum wi th 70 % a lcoh ol. • The bottle has been pre-ma rked with 5mli ncrements. Markthedesiredll volumelevelonthebottlefor1 0ml. 3.VenipunctureandBottleInoculation Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit • Collect all nec ess ary materials for a direct d raw : BacT/ALERT ® Blood Culture Collection Procedure Availability WorkSafeBloodCultureCollectionKit 279 102-2791 05 * Saf-THolder ® Blood Cul tur e Devic e ( Ada ptor Ca p a nd Ins ert ) 1 00/ cas e 96004 ** * seebioMérieuxcatalogue ** seeSmithsMedicalcatalogue bioMérieux, Inc. 100RodolpheStreet bioMérieux Canada, Inc. 7815Henri-BourassaBoulevardOuest © 2 0 0 8 B I O M É R I E U X , I N C . B A C T / A L E R T ® A N D B A C T / A L E R T ® 3 D A R E R E G I S T E R E D T R A D E M A R K S O F B I O M É R I E U X . S A F T H O L D E R ® I S A R E G I S T E R E D T R A D E M A R K O F T H E S M I T H S M E D I C A L F A M I L y O F C O M p A N I E S . C H L O R A S C R U B I S A T R A D E M A R K O F p D I . p R I N T E D I N U S A . B T A 1 2 5 0 8 . Tips ’n’ Hints •Recommendedbloodtobrothratiois1:5 to1 :1 0.Asthevolumeofblooddrawnis increased,theyieldofpositivecultures increases.Optimally,20mlofbloodshould bedrawnfromadults(10mlperbottle). •Whenlabelingthebottles,donotcoverthe peel-offsectionofthebarcodelabelsorthe lotnumbers. •Orientadditionalpatientlabelverticallyfor scanningefciency. •Forbestvolumecontrol,marklllevelon sideofbottlepriortocollection. •Donotoverllthebottles,asthismaycause falsepositivereadings. •WhenusingtheSaf-THolder ® BloodCulture Device(AdaptorCapandInsert),besure luerisconnectedrmlyandtheneedle isstraightwhenenteringandleavingthe septum.Twistingorturningthebottlemay increasethechancethatthesheathmaynot retractandreseal. •Toavoidcontaminationofthebloodculture sample,inoculatebloodculturebottlesrst. Thenlladditionalbloodcollectiontubes. Septicemiaoccursinapproximately200,000patie ntseachyear,andbetween30and50%ofthosepatients die.Becausethe consequencesofsepticemiaaresoserious,rapidandaccuratediagnosisofthisdisorderiscritical. TheBloodCultureisthebestdiagnosti ctoolfordetectingsepticemia,andit’s absolutelyimperativethatthebloodspecimenbe collectedproperlytoprovideclinicallyvaluableresults. BacT/ALERT ® 3Dinstrumentsareautomatedsystemsthatincubate,shakeandmonitorculturebottlesforsignsofmicrobialgrowth. Samplesfrompatientssuspectedforsepti cemiaareinoculatedintoBacT/ALERTculturebottlesfor monitoring.Thebottlesarecolor codedforeasyidenticationofmediatypeandaremadeofplasticforaddedsafety. Performvenipuncture. Whentheneedleisin thevein,secureitwith tapeorholditinplace. 2. PlaceAdaptercaponthe aerobic(blueorgreenlabel) BacT/ALERTculturebottle septumandpressdown topenetrateandobtain bloodow.Holdthe AdapterCapdownon thebottle. 3. ECI E G I H Usingthellindicator linesonthelabel,obtain 8-10mlofblood.Move theAdapterCapfrom theaerobicbottletothe anaerobic(purpleor orangelabel)bottle(if required)andcontinue thecollection. 4. ECIE G I H Ifadditionalbloodis requiredforothertests, placetheAdapterInsert intotheAdapterCap andsnapintoplace. Thismakesthecap compatiblewithvacuum collectiontubes. 5. Whennaltubeislled, removetourniquet. Terminatethe venipuncture,activate thesafetyshield,grab thewingwithonehand andslidethewingsback untilitlocksinplace. 6. ECIE G I H Disposeofwing andadaptercapin asharpscontainer. 7 . SPECIMENBAG BIOHAZARD Labelspecimensandplace inplasticbiohazardzip lockbag.Bottlescannow besafelytransportedvia pneumatictubesystem. 8. OR GatherTourniquet,BacT/ALERT ® bottles, Saf-THolder ® Adapter,wing,Chlorascrub™ Swabstick,gauze,alcoholpreppads. 1a. OpentheWorkSafeBloodCultureCollection Kitwhichcontainsallthenecessarymaterials inasingle,convenientpackage. 1b.