Working with Minitab 16

Working with Minitab

statistical tools to improve the yield in

fermentative production process

By

Deepak K. PatilEmail- [email protected]

Microbiologist starts with some medium and set of conditions for the expression of metabolite or activity of interest

• MICROBIOLOGIST

• MICROBE– MEDIUM

– SET OF CONDITIONS

» TEMP

» pH

• METABOLITE/ACTIVITY OF INTEREST

– ANTIBIOTIC

– ANTICANCER AGENT

– ENZYMES

TASK IS TO IMPROVE THE

YIELD OF

‘DESIRED PRODUCT’

Working with Minitab for optimization

• Select the factors to be optimized

• Run PBD to screen the factors

• With screened factors, run CCD

Remember !!!

• PBD is for screening the factors

• CCD is for optimizing the screened factors

For designing PBD

• Select the factors

• Decide their levels i.e. 0, +1 & -1

• Depending upon the number of factors select the

design

For designing CCD

• Decide the levels of screened factors

depending upon the main effects plot

• Select the design

For your implementation

Remember that, there are coded

values in this presentation, which

can be replaced by real ones

Thanks!!!