Wolbachia – The biology of cytoplasmic incompatibility By, B.C. Muthubharathi, III B.Sc.,Biotechnology.
Wolbachia – The biology of cytoplasmic incompatibility
By,B.C. Muthubharathi,III B.Sc.,Biotechnology.
What is Wolbachia?Systematic classification Kingdom : Bacteria Phylum : Proteobacteria Class : Alphaproteobacteria Order : Rickettsiales Family : Rickettsiaceae Genus : Wolbachia Species : melophagi persica pipientis
Infectsarthropod(insects), NematodesParasitic microbe1924 , Marshall hertig
Biocontrol agentBiopesticide
Is it an Insecticide?IntracellularInfect reproductive tissueParthenogenesisFeminizationMale KillingCytoplasmic IncompatibilityAlter the Phenotype
It affects• Cherry Fruit Fly, • Mosquitoes, • Mites, Isopods etc.,
Cytoplasmic Incompatibility
• Results in sperm and egg unable to form viable offspring
• Changes in gamete cells• Leads to delayed entry of male pronucleus• Embryonic mortality
Cellular Mechanism28 Strains are identified WMelPop-CLA : Reduce life span of adult WMel : Induce reproductive phenotypeModification - SpermatogenisisRescue - After fertilzationSpematocytes and spermatidesSomatic cyst cell surrounding germcell
Prevent transmission of arbovirus , Human parasitesProtect infected insects from RNA virus
Role of Wolbachia in Reproduction of insects
Areas affected with Dengu
Cont..
Unidirectional CI: Mating of infected males and uninfected females results in
CI. All other crosses are compatible.
Bidirectional CI: Mating of males infected with Wolbachia strain I and females
infected by Wolbachia strain II (and vice versa) results in CI.