Whole Genome Sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection Comparative Microbial Genomics: A Bioinformatics Approach 5th October 2005 Ana Rita Domingues Boumediene Soufi Francisco Roque Gianni Panagiotou
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Whole Genome Sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection Comparative Microbial Genomics:
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Whole Genome Sequence of
Staphylococcus saprophyticus reveals the pathogenesis of
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Abstract
PNAS vol 102, no37, September 13, 2005
Outline
Brief introduction
Genome features
Comparative genomics
Results and discussion Summary
Staphylococcus saprophyticus characteristics and importance
Schematic circular diagrams of the S. Saprophyticus ATCC 15305 chromosome (Kuroda et al. 2005)
S. saprophyticus ATCC 15305 genome characteristics - results
• 2,446 ORFs
• Two IS431 elements
• 9 putative transposases
• 2 staphylococcal cassette chromosomes (SCC15305RM and SCC15305cap)
• Genomic island (vSs15305)
• 2 plasmids (pSSP1 - 38.4kb and pSSP2 - 22.9kb)
Comparison of general features of the whole genome sequenced for the three strains of Staphylococci (Kuroda et al. 2005)
Table 2. Comparison of general features of the whole genome sequenced staphylococci
Mobile genomic islands of S. saprophyticus ATCC 15305 (Kuroda et al. 2005)
Orthologous vs Paralogous
Homologous sequences are orthologous if they were separated by a speciation event: if a gene exists in a species, and that species diverges into two species, then the copies of this gene in the resulting species are orthologous;
Homologous sequences are paralogous if they were separated by a gene duplication event: if a gene in an organism is duplicated, then the two copies are paralogous;
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Orthologous classification of predicted ORFs compared with those of S. aureus N315 and S. epidermidis ATCC 12228 (Kuroda et al. 2005)
Paralog expansion of transport systems related to urine environment (Kuroda et al. 2005)
Adherence to eukaryotic cell by UafA (SSP0135) (Kuroda et al. 2005)
Hemagglutination:
Positive – well 3, 4 and 5
Negative – well 1, 2 and 6
None of the virulence factors found in S. aureus
Summary of the results
S. saprophyticus ATCC 15305
Contains elaborate paralog expansion of transport systems
Presence of a cell wall-anchored protein for adherence to the urinary tract