What a Great Time to Teach & Do Research with Undergrads!! So much data! *** So much for me & my students to do!!!*** So many questions! So many great tools! Too few researchers! As of 1/3/08: Domain Published Ongoing Archaea 50 68 Bacteria 577 1628 Eucarya 77 891 (+ 114 metagenomes + GEBA plan)
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What a Great Time to Teach & Do Research with Undergrads!!
As of 1/3/08: Domain Published Ongoing Archaea5068 Bacteria5771628 Eucarya77891 (+ 114 metagenomes + GEBA plan). What a Great Time to Teach & Do Research with Undergrads!!. So many questions! So many great tools! Too few researchers!. So much data!. - PowerPoint PPT Presentation
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What a Great Time to Teach & Do Research with Undergrads!!
So much data!
*** So much for me & my students to
do!!!***
So many questions!So many great tools!Too few researchers!
Functional GenomicsExample = 2 Aconitases in Agrobacterium C58
wt acnA-
wildtype A. tumefaciensfrom LB plate (pH7)
A. tumefaciens acnA- mutantfrom LB plate (pH7)
• One group wanted to look at motility!?
• Motility is one process regulated post-transcriptionally by apo-AcnB in E. coli
Functional GenomicsForward Genetics Screens
Transposonmutagenesis
Mutant screening & characterization
Sequence off of Tn end to identify mutated gene
Recovery of Tn insertion site
• Auxotrophs are easy to screen & connect to larger issues of metabolism & nutrition - learn bacterial genetics, mutagenesis, connect genes to enzymes to pathways
• If needed, college students physically map insertions - restriction mapping of DNA
• obtain sequences at insertion sites - learn DNA sequence analysis, connect genotype to phenotype
Forward Genetic ScreensHigh School Students Can Do It
• Real world = multiple classes since 2002 from 5 area high schools
• Each session lasted 3-5 days
• Students generated mutants, screened for phenotypes, recovered Tn insertion sites for sequencing, & learned some bioinformatics
• high school students + 11 Hiram students generated & screened 10,500 mutants for 10 different phenotypes, & identified 110 mutants worthy of further study
Forward Genetic ScreensHiram Genomics Academy
• 79 students from high schools in OH, PA, MI, & IN spread over 5 summer sessions in 2006 & 2007