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Investigation about Log Reduction ofBlood Contamination byWasher-Disinfectors
• We tried to figure out the log reduction of blood contamination by washer-disinfectors with practical cleaning cycles, using the test pieces contaminated quantitatively by sheep blood.
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Hierarchic Structure of Working Group
• JAPANESE SOCIETY OF MEDICAL INSTRUMENTATION ( JSMI )
–Accrediting Committee of Reprocessing Technicians
–Working Group of Cleaning Evaluation
The WG was set up in April 2006 for cleanliness evaluation with 11 group members assigned.
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Members of Working GroupRyo Fushimi Osaka Univ. Hosp.
Measurement Result in LaboratorySetting Parameters of Main Wash Residual
ProteinAverage
(μg)℃ min. Detergent (type, v%, pH)
90 5 Alkaline 0.3 11.3 30 30.845
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23
20
60 10 Alkaline 0.3 11.3 37 31.621
34
29
37
50 10 Enzymatic 0.5 8.2 32 42.458
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Measurement Result in HospitalsSetting Parameters of Main Wash Residual
ProteinAverage
(μg)℃ min. Detergent (type, v%, pH)
93 10 Alkaline 0.3 11.3 97 68.75851
93 10 Alkaline 0.3 11.3 20 29.73732
60 8 Alkaline 0.5 12.0 21 16.01413
40 5 Alkaline 0.5 11.5 28 28.32631
pretreatment: immersing into enzymatic detergent solution (0.8 v%) for 5 min. prior to processing by WD
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Conclusion
• The measurements of the residual protein in the laboratory varied between 20 and 58 μg and the rate of protein decrease showed 1/100,000 down to 1/34,000.
• The measurements of the residual protein in the hospitals varied between 13 and 97 μg and the rate of protein decrease showed 1/153,000 down to 1/20,000.
• Washer-disinfectors have power 5 of the log reduction factor of sheep blood.