cancerres.aacrjournals.org · Web view(C) Confocal analysis of A-FABP expression and macrophage infiltration in benign and malignant breast tissues. Scale bars represent 50µm. Scale

Supplementary Figure 3 Analysis of A-FABP expression in adipocytes cocultured with

different tumor cells and in TAM of breast tissues. (A-B) Subcutaneous adipocytes were co-

cultured with indicated tumor cells in a transwell insert (A) for 24h. A-FABP expression in

adipocytes co-cultured with or without indicated tumor cells was analyzed by real-time PCR (B)

(*, p<0.05). (C) Confocal analysis of A-FABP expression and macrophage infiltration in benign

and malignant breast tissues. Scale bars represent 50µm.

Supplementary Figure 4 Analyses of macrophage depletion in the spleen and immune cell

infiltration in WT and A-FABP-/- treated with clodronates or control liposomes. (A) WT

and A-FABP-/- mice were i.p. injected with 100µl clodronates or control liposomes. Depletion

of macrophages in the spleen was analyzed 7 days after the injection by flow cytometric staining

with anti-CD11b and anti-F4/80 antibodies (n=5/group) (*p<0.05). (B-E) Flow cytometric

analysis of CD4+ T cells (B), CD8+ T cells (C), CD19+ B cells (D) and NK1.1+ cells (E) in the

tumor stroma of mice treated with clodronate or control liposomes.