Muscle Injury Associated Elevated Oxidative Stress And Abnormal Myogenesis In Patients With Idiopathic Scoliosis Jiong Li, Mingxing Tang, Guanteng Yang, Longjie Wang, Qile Gao, Hongqi Zhang*. * Corresponding author: Hongqi Zhang, [email protected]. Department of Spine Surgery, Xiangya Hospital, Central South University, No. 87, Xiangya Road, Changsha, Hunan, China, 410008. 1 / 42
42
Embed
· Web viewAfter preincubation of 15 minutes (min) in buffer with 200 mM Tris base, 18 mM CaCl 2, pH 4.3, the sections were incubated for 1 hour (h) in 200 mM Tris base, 18 mM CaCl
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Muscle Injury Associated Elevated Oxidative Stress And Abnormal Myogenesis In Patients With
This study has been conducted adhering to the principles of the Declaration of Helsinki II and approved
by the medical ethics committee of Xiangya Hospital, Central South University (ethical code:
201703358). Written informed consent was obtained from all the study participants or their parent or
guardian respectively.
Acknowledgements
The authors thank all the staf of the Department of Spine Surgery, Xiangya Hospital, Central South
University for their dedicated assistance in patient sample collection. This work was supported by the
National Natural Science Foundation of China (No.81772298) and the National Natural Science
Foundation of Hunan Province, China (No.2018JJ3848 and No.2017JJ3338).
Competing interests
The authors declare that they have no competing interests.
References
1. Buckingham M. Skeletal muscle formation in vertebrates. Current opinion in genetics & development. 2001; 11: 440-8.2. Perry RL, Rudnick MA. Molecular mechanisms regulating myogenic determination and differentiation. Frontiers in bioscience : a journal and virtual library. 2000; 5: D750-67.3. Schiaffino S, Reggiani C. Fiber types in mammalian skeletal muscles. Physiological reviews. 2011; 91: 1447-531.4. Duan Y, Li F, Tan B, Yao K, Yin Y. Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes. Obesity reviews : an official journal of the International Association for the Study of Obesity. 2017; 18: 647-59.5. Wilke HJ, Wolf S, Claes LE, Arand M, Wiesend A. Influence of varying muscle forces on lumbar intradiscal pressure: an in vitro study. Journal of biomechanics. 1996; 29: 549-55.6. Yagi M, Hosogane N, Watanabe K, Asazuma T, Matsumoto M, Keio Spine Research G. The
20 / 33
paravertebral muscle and psoas for the maintenance of global spinal alignment in patient with degenerative lumbar scoliosis. The spine journal : official journal of the North American Spine Society. 2016; 16: 451-8.7. Wajchenberg M, Martins DE, Luciano Rde P, Puertas EB, Del Curto D, Schmidt B, et al. Histochemical analysis of paraspinal rotator muscles from patients with adolescent idiopathic scoliosis: a cross-sectional study. Medicine. 2015; 94: e598.8. Linek P, Saulicz E, Kuszewski M, Wolny T. Ultrasound Assessment of the Abdominal Muscles at Rest and During the ASLR Test Among Adolescents With Scoliosis. Clinical spine surgery. 2017; 30: 181-6.9. Yang HS, Yoo JW, Lee BA, Choi CK, You JH. Inter-tester and intra-tester reliability of ultrasound imaging measurements of abdominal muscles in adolescents with and without idiopathic scoliosis: a case-controlled study. Bio-medical materials and engineering. 2014; 24: 453-8.10. Watanabe K, Ohashi M, Hirano T, Katsumi K, Shoji H, Mizouchi T, et al. The Influence of Lumbar Muscle Volume on Curve Progression After Skeletal Maturity in Patients With Adolescent Idiopathic Scoliosis: A Long-Term Follow-up Study. Spine deformity. 2018; 6: 691-8 e1.11. Li J, Huang J, Li JS, Chen H, Huang K, Zheng L. Accumulation of endoplasmic reticulum stress and lipogenesis in the liver through generational effects of high fat diets. Journal of hepatology. 2012; 56: 900-7.12. Hintz CS, Coyle EF, Kaiser KK, Chi MM, Lowry OH. Comparison of muscle fiber typing by quantitative enzyme assays and by myosin ATPase staining. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 1984; 32: 655-60.13. Li Q, Li QQ, Jia JN, Cao S, Wang ZB, Wang X, et al. Sodium Valproate Ameliorates Neuronal Apoptosis in a Kainic Acid Model of Epilepsy via Enhancing PKC-Dependent GABAAR gamma2 Serine 327 Phosphorylation. Neurochemical research. 2018; 43: 2343-52.14. Tang Y, Li S, Zhang P, Zhu J, Meng G, Xie L, et al. Soy Isoflavone Protects Myocardial Ischemia/Reperfusion Injury through Increasing Endothelial Nitric Oxide Synthase and Decreasing Oxidative Stress in Ovariectomized Rats. Oxidative medicine and cellular longevity. 2016; 2016: 5057405.15. Shang Q, Bao L, Guo H, Hao F, Luo Q, Chen J, et al. Contribution of glutaredoxin-1 to S-glutathionylation of endothelial nitric oxide synthase for mesenteric nitric oxide generation in experimental necrotizing enterocolitis. Translational research : the journal of laboratory and clinical medicine. 2017; 188: 92-105.16. Dmitrieva RI, Lelyavina TA, Komarova MY, Galenko VL, Ivanova OA, Tikanova PA, et al. Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations. Stem cells international. 2019; 2019: 5690345.17. Liu S, Gao F, Wen L, Ouyang M, Wang Y, Wang Q, et al. Osteocalcin Induces Proliferation via Positive Activation of the PI3K/Akt, P38 MAPK Pathways and Promotes Differentiation Through Activation of the GPRC6A-ERK1/2 Pathway in C2C12 Myoblast Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 2017; 43: 1100-12.18. Jiang B, Wang K, Liang P, Xiao W, Wang H, Xiao X. ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells. The FEBS journal. 2009; 276: 2615-24.
21 / 33
19. Wang YJ, Yu HG, Zhou ZH, Guo Q, Wang LJ, Zhang HQ. Leptin Receptor Metabolism Disorder in Primary Chondrocytes from Adolescent Idiopathic Scoliosis Girls. International journal of molecular sciences. 2016; 17.20. Singh S, Canseco DC, Manda SM, Shelton JM, Chirumamilla RR, Goetsch SC, et al. Cytoglobin modulates myogenic progenitor cell viability and muscle regeneration. Proceedings of the National Academy of Sciences of the United States of America. 2014; 111: E129-38.21. Di Meo S, Reed TT, Venditti P, Victor VM. Role of ROS and RNS Sources in Physiological and Pathological Conditions. Oxidative medicine and cellular longevity. 2016; 2016: 1245049.22. Le Moal E, Pialoux V, Juban G, Groussard C, Zouhal H, Chazaud B, et al. Redox Control of Skeletal Muscle Regeneration. Antioxidants & redox signaling. 2017; 27: 276-310.23. Abidi SL. High-performance liquid chromatographic resolution and quantification of a dilactonic antibiotic mixture (antimycin A). Journal of chromatography. 1982; 234: 187-200.24. Konieczny MR, Senyurt H, Krauspe R. Epidemiology of adolescent idiopathic scoliosis. Journal of children's orthopaedics. 2013; 7: 3-9.25. de Souza FI, Di Ferreira RB, Labres D, Elias R, de Sousa AP, Pereira RE. Epidemiology of adolescent idiopathic scoliosis in students of the public schools in Goiania-GO. Acta ortopedica brasileira. 2013; 21: 223-5.26. Hengwei F, Zifang H, Qifei W, Weiqing T, Nali D, Ping Y, et al. Prevalence of Idiopathic Scoliosis in Chinese Schoolchildren: A Large, Population-Based Study. Spine. 2016; 41: 259-64.27. Fadzan M, Bettany-Saltikov J. Etiological Theories of Adolescent Idiopathic Scoliosis: Past and Present. The open orthopaedics journal. 2017; 11: 1466-89.28. Zoabli G, Mathieu PA, Aubin CE. Magnetic resonance imaging of the erector spinae muscles in Duchenne muscular dystrophy: implication for scoliotic deformities. Scoliosis. 2008; 3: 21.29. Maffulli N. Histochemical and physiological studies in idiopathic scoliosis. Italian journal of orthopaedics and traumatology. 1990; 16: 61-71.30. Khosla S, Tredwell SJ, Day B, Shinn SL, Ovalle WK, Jr. An ultrastructural study of multifidus muscle in progressive idiopathic scoliosis. Changes resulting from a sarcolemmal defect at the myotendinous junction. Journal of the neurological sciences. 1980; 46: 13-31.31. Fry CS, Porter C, Sidossis LS, Nieten C, Reidy PT, Hundeshagen G, et al. Satellite cell activation and apoptosis in skeletal muscle from severely burned children. The Journal of physiology. 2016; 594: 5223-36.32. Tamilarasan KP, Temmel H, Das SK, Al Zoughbi W, Schauer S, Vesely PW, et al. Skeletal muscle damage and impaired regeneration due to LPL-mediated lipotoxicity. Cell death & disease. 2012; 3: e354.33. Min K, Lawan A, Bennett AM. Loss of MKP-5 promotes myofiber survival by activating STAT3/Bcl-2 signaling during regenerative myogenesis. Skeletal muscle. 2017; 7: 21.34. Battistelli M, Salucci S, Guescini M, Curzi D, Stocchi V, Falcieri E. Skeletal Muscle Cell Behavior After Physical Agent Treatments. Current pharmaceutical design. 2015; 21: 3665-72.35. Kitajima Y, Suzuki N, Nunomiya A, Osana S, Yoshioka K, Tashiro Y, et al. The Ubiquitin-Proteasome System Is Indispensable for the Maintenance of Muscle Stem Cells. Stem cell reports. 2018.36. Jiang J, Li P, Ling H, Xu Z, Yi B, Zhu S. MiR-499/PRDM16 axis modulates the adipogenic differentiation of mouse skeletal muscle satellite cells. Human cell. 2018; 31: 282-91.37. Anderson EJ, Neufer PD. Type II skeletal myofibers possess unique properties that potentiate
22 / 33
mitochondrial H(2)O(2) generation. American journal of physiology Cell physiology. 2006; 290: C844-51.38. Sandri M. Signaling in muscle atrophy and hypertrophy. Physiology. 2008; 23: 160-70.39. Lin J, Wu H, Tarr PT, Zhang CY, Wu Z, Boss O, et al. Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature. 2002; 418: 797-801.40. Arany Z, Lebrasseur N, Morris C, Smith E, Yang W, Ma Y, et al. The transcriptional coactivator PGC-1beta drives the formation of oxidative type IIX fibers in skeletal muscle. Cell metabolism. 2007; 5: 35-46.41. Scarpulla RC, Vega RB, Kelly DP. Transcriptional integration of mitochondrial biogenesis. Trends in endocrinology and metabolism: TEM. 2012; 23: 459-66.42. Baldelli S, Aquilano K, Ciriolo MR. PGC-1alpha buffers ROS-mediated removal of mitochondria during myogenesis. Cell death & disease. 2014; 5: e1515.43. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiological reviews. 2007; 87: 315-424.44. Alfadda AA, Sallam RM. Reactive oxygen species in health and disease. Journal of biomedicine & biotechnology. 2012; 2012: 936486.45. Fleissner F, Thum T. Critical role of the nitric oxide/reactive oxygen species balance in endothelial progenitor dysfunction. Antioxidants & redox signaling. 2011; 15: 933-48.46. Mannion AF, Dumas GA, Cooper RG, Espinosa FJ, Faris MW, Stevenson JM. Muscle fibre size and type distribution in thoracic and lumbar regions of erector spinae in healthy subjects without low back pain: normal values and sex differences. Journal of anatomy. 1997; 190 ( Pt 4): 505-13.47. Rantanen J, Rissanen A, Kalimo H. Lumbar muscle fiber size and type distribution in normal subjects. European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society. 1994; 3: 331-5.
23 / 33
Figures legends
Fig. 1. Histopathological analysis of paraspinal muscles in IS patients and control group. H&E stained
paraspinal muscle sections of IS patients and control group (A); black arrow, necrosis; blue arrow,
infiltration of immune cells; green arrow, central nuclei. B-D, Quantity evaluation of muscle injury,
percentage ratio of infiltration of immune cells to total cells, and percentage of central nuclei and.
n=15-20, *p<0.05.
Fig. 2. Increased apoptosis in paraspinal muscles of IS patients. A, TUNEL stained paraspinal muscle
sections of IS patients and control group, 200×, n=15-20. B, Quantity evaluation of percentage of
TUNEL positive muscle cells, n=15-20. C, WB analysis of apoptosis related proteins and quantification