RESULTS & GRAPHICS/CHARTS Cleaned textile, two styles: We tested textiles that were prepared in two different ways different insulator coverings) in order to see which way produced usable results. The textile with the larger surface area produced a more linear graph with a higher R-squared value than the textile with the smaller surface area. (show graphs and data as needed). Ag-Pt textile: We ran sensing tests on three platinum textiles with different amounts of silver deposited on top of it. Prior research has shown that silver has antimicrobial properties which is a desirable trait. The results were linear in most cases and… (add more after looking at graphs/statistics for silver sensing) DISCUSSION The data we have collected from our Amperometric i-t curves indicate encouraging results for the future development of nanometal-based textile biosensors. We applied two coats of nail polish on the textile to see which one had an impact on the cleaning. We found out that the textile with half nail polish applied on it had better result because the sensing curve(R- square) was .963, while the textile with full nail polished applied only had .764. The closer the sensing curve is to 1, the better your model is. In summary, the textile with less nail polish on it had more surface area exposed, which led to better cleaning of textile electrode. This cleaning process typically led to better H 2 O 2 sensitivity and a more linear correlation between current response and H 2 O 2 concentration. The last testing we did was with the textiles that had silver deposited on it. We did the silver deposition because silver is anti-microbial. We were trying to see whether time was a factor in sensing with silver. We found out that it was because the less silver applied, the better the sensing result where. For the textile that had one minute silver deposited on to it, the sensing curve was .964, for the 3 minute textile, It was .902, and for the 5 minute, it was .873. Addition of silver nanoparticles to the textile increases its antimicrobial properties but it also weaken the electros sensitivity. In future work, the ration of silver to platinum nanoparticle will be optimized in order to provide both a sensitivity sensor and a sensor that does not biofoul. ABSTRACT For our project, we were developing a fabricated nano-platinum sensor that will be embedment in clothing. We did this by depositing platinum onto a textile and using two different methods of nail polish to see which one had the better surface area. We also deposited Silver on to three different textiles and tested it because silver has antimicrobial properties. We were able to conclude that the less silver applied on textile the better sensing. The addition of silver adds antimicrobial but it weakness sensitivity The material presented here is based upon work supported by the National Science Foundation under Award No. EEC-0813570. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Wearable Electrochemical Biosensors Fahmo Mohamed ,Ogue Addeh, Makenzie Petersen, Gabe Wright Mentor: Jonathan Claussen Ph.D Graduate Mentors: Suprem Das, Shaowei Ding METHODS 1) Deposit platinum nanowires on fabric. Add 54 ml of DI water to 6ml of formic acid and 300 milligrams moles of chlorplatinic acid hexa- hydrate. Measured the pH to make sure it was between 1.55 and 1.6. 2) Insert in white fabric in the yellow solution. Wait 24 hours for the solution to turn clear and the fabric to turn black. 3) Clean with nitric acid and DI water than t ake 50 cycles from -2.0 to -1.5v using sulfuric acid (concentrated HNO3) . 4) Apply two coats of acrylic insulator (i.e., nail polish) and tested it to see which one was better. 5) Deposit silver nanoparticles. Use .1M of KNO 3 with 1.0mm of AgNO3 as our solution. 6) Use three textiles, do a 1 minute, 3 minute, and 5 minute sensing on the amperometric i-t curve. 7) Start Hydrogen peroxide (H 2 O 2 ) sensing on the textiles with silver deposition. BACKGROUND • Saliva • Sweat • Subcutaneous Fluid • Blood RESULTS & GRAPHICS/CHARTS ACKNOWLEDGEMENT Thank you to Jonathan Claussen Ph.D, Shaowei Ding, Gabe Wright Ogue Addeh, and Makenzie Petersen Uncleaned sample Half nail polished Full nail polished No strong prevalenc e of “steps 1 minute .7V 3 minute .7V 5 minute .7V No strong prevalence of “steps H 2 O 2 sensing Glucose sensing Monitors heart rate and daily activity Tracks activity exercise, food, weight and sleep Monitor people's vital signs while they are up and about performing their normal daily activities REFERENCES Yixian Wang, ZunZhong Ye, Jianfeng Ping, Shunru Jing, Yibin Ying “ Development of an aptamer-based impedimetirc bioassay using microfluidic system and magnetic separation for protein detection” 1500X 5000X 50000X 50 cycles 250 cycles 500 cycles